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The map label for this gene is dusB [H]

Identifier: 120609588

GI number: 120609588

Start: 971368

End: 972429

Strand: Direct

Name: dusB [H]

Synonym: Aave_0894

Alternate gene names: 120609588

Gene position: 971368-972429 (Clockwise)

Preceding gene: 120609587

Following gene: 120609589

Centisome position: 18.15

GC content: 70.62

Gene sequence:

>1062_bases
ATGCAGATCGGCCACATTCCCCTGGCGAACCGCCTCTTCGTCGCTCCCATGGCGGGGGTGACGGACCGGCCGTTCCGCCA
GCTCTGCAAGGCCCTGGGCGCGGGCTACGCCGTGAGCGAGATGGTGACCTCGCGCAAGGACCTGTGGAACAGCCTGAAGA
CCTCGCGCCGTGCCAACCACGAGGGCGAGCCCGGCCCGATCGCCGTGCAGATCGCCGGCACCGACGCGGCCATGATGTCC
GAGGCCGCGCTCTACAACATCGACCGCGGCGCGGAGATCATCGACATCAACATGGGCTGCCCCGCCAAGAAGGTCTGCAA
CAAGTGGGCGGGCTCGGCCCTCATGCAGAACGAGGCGCTGGCCGTGGAGATCGCGCAGGCCGTCGTCGATGCCTGCGCAC
CGCGCAACGTGCCCGTCACGCTCAAGATGCGCACCGGCTGGTGCCAGCAGCACCGGAATGCGGTGCAGCTCGCGCGCGCG
TTCGAGGGCGTGGGCATCCAGATGCTGACGGTGCACGGCCGCACGCGCGAGCAGGGATACAAGGGCCACGCCGAGTACGA
CACCATCGCCGCCGTGAAGGCCGCCGTGCGCGTGCCGGTGGTGGCCAACGGCGACATCGATTCGCCCGAGAAGGCGAAGG
CGGTGCTTGCCGCAACGGGAGCGGACGCGATCATGATCGGCCGCGCGGCGCAGGGCCGGCCCTGGATCTTCCGCGAGATC
GGCCACTACCTCGCCACGGGCGAGCACCTGGCGCCGCCGCTGGTGGCCGAGGTGCGGCGCCTGCTGCTGGACCACCTGCA
GGACCACTACGCGCTCTACGGCGAGGCCACGGGCGTGCGCAGCGCACGCAAGCACATCGCCTGGTACGTGCGCGCGCTGC
CGGGCGGCGAGGCGTTCCGGCAGCACATCAACACCATCGACGACTGCGCCGCCCAGTGGCAGGCCGTCGCGCTGTTCTTC
GAGGAGCTGGGCCGGCACATGGACCGCCTCCCCGCAGCGCCGCCCGAGGGGGCCGGCACGGATACCGACGATACGCAGGA
ACCACAAGGACTGCCCGCATGA

Upstream 100 bases:

>100_bases
CCCGGGTCCGCGTCCCCTGCTTTTCATTTGCTGAAAATTTAAGCAGGTACAATCCCGCCTCTTTTTTCTTCCGCCTGTTT
TTTCAGCACCCCCGCGCGCC

Downstream 100 bases:

>100_bases
GCAACAAGAACATCGAAGACTGCGTGCGCGAGAGCCTGCAGGGATATTTTCGCGACCTCGGGGGCGAGACCCCCGACGGC
ATGTACGACATGCTCGTGCG

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MQIGHIPLANRLFVAPMAGVTDRPFRQLCKALGAGYAVSEMVTSRKDLWNSLKTSRRANHEGEPGPIAVQIAGTDAAMMS
EAALYNIDRGAEIIDINMGCPAKKVCNKWAGSALMQNEALAVEIAQAVVDACAPRNVPVTLKMRTGWCQQHRNAVQLARA
FEGVGIQMLTVHGRTREQGYKGHAEYDTIAAVKAAVRVPVVANGDIDSPEKAKAVLAATGADAIMIGRAAQGRPWIFREI
GHYLATGEHLAPPLVAEVRRLLLDHLQDHYALYGEATGVRSARKHIAWYVRALPGGEAFRQHINTIDDCAAQWQAVALFF
EELGRHMDRLPAAPPEGAGTDTDDTQEPQGLPA

Sequences:

>Translated_353_residues
MQIGHIPLANRLFVAPMAGVTDRPFRQLCKALGAGYAVSEMVTSRKDLWNSLKTSRRANHEGEPGPIAVQIAGTDAAMMS
EAALYNIDRGAEIIDINMGCPAKKVCNKWAGSALMQNEALAVEIAQAVVDACAPRNVPVTLKMRTGWCQQHRNAVQLARA
FEGVGIQMLTVHGRTREQGYKGHAEYDTIAAVKAAVRVPVVANGDIDSPEKAKAVLAATGADAIMIGRAAQGRPWIFREI
GHYLATGEHLAPPLVAEVRRLLLDHLQDHYALYGEATGVRSARKHIAWYVRALPGGEAFRQHINTIDDCAAQWQAVALFF
EELGRHMDRLPAAPPEGAGTDTDDTQEPQGLPA
>Mature_353_residues
MQIGHIPLANRLFVAPMAGVTDRPFRQLCKALGAGYAVSEMVTSRKDLWNSLKTSRRANHEGEPGPIAVQIAGTDAAMMS
EAALYNIDRGAEIIDINMGCPAKKVCNKWAGSALMQNEALAVEIAQAVVDACAPRNVPVTLKMRTGWCQQHRNAVQLARA
FEGVGIQMLTVHGRTREQGYKGHAEYDTIAAVKAAVRVPVVANGDIDSPEKAKAVLAATGADAIMIGRAAQGRPWIFREI
GHYLATGEHLAPPLVAEVRRLLLDHLQDHYALYGEATGVRSARKHIAWYVRALPGGEAFRQHINTIDDCAAQWQAVALFF
EELGRHMDRLPAAPPEGAGTDTDDTQEPQGLPA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=318, Percent_Identity=30.188679245283, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI239788462, Length=318, Percent_Identity=29.874213836478, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=30.2222222222222, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI40807366, Length=232, Percent_Identity=30.6034482758621, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI8923374, Length=252, Percent_Identity=27.3809523809524, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1789660, Length=323, Percent_Identity=54.4891640866873, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=30.7053941908714, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI145693211, Length=313, Percent_Identity=26.8370607028754, Blast_Score=81, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=317, Percent_Identity=28.391167192429, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=291, Percent_Identity=29.553264604811, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=246, Percent_Identity=29.2682926829268, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=256, Percent_Identity=28.125, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=262, Percent_Identity=28.2442748091603, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=27.6315789473684, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=238, Percent_Identity=31.9327731092437, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585320, Length=243, Percent_Identity=32.5102880658436, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24580595, Length=239, Percent_Identity=31.7991631799163, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19920448, Length=239, Percent_Identity=31.7991631799163, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45549423, Length=279, Percent_Identity=27.2401433691756, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38202; Mature: 38202

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGHIPLANRLFVAPMAGVTDRPFRQLCKALGAGYAVSEMVTSRKDLWNSLKTSRRANH
CCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EGEPGPIAVQIAGTDAAMMSEAALYNIDRGAEIIDINMGCPAKKVCNKWAGSALMQNEAL
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCHHHHCCCHH
AVEIAQAVVDACAPRNVPVTLKMRTGWCQQHRNAVQLARAFEGVGIQMLTVHGRTREQGY
HHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCC
KGHAEYDTIAAVKAAVRVPVVANGDIDSPEKAKAVLAATGADAIMIGRAAQGRPWIFREI
CCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHEEEEECCCCEEEEECCCCCCCHHHHHH
GHYLATGEHLAPPLVAEVRRLLLDHLQDHYALYGEATGVRSARKHIAWYVRALPGGEAFR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHH
QHINTIDDCAAQWQAVALFFEELGRHMDRLPAAPPEGAGTDTDDTQEPQGLPA
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQIGHIPLANRLFVAPMAGVTDRPFRQLCKALGAGYAVSEMVTSRKDLWNSLKTSRRANH
CCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EGEPGPIAVQIAGTDAAMMSEAALYNIDRGAEIIDINMGCPAKKVCNKWAGSALMQNEAL
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCHHHHCCCHH
AVEIAQAVVDACAPRNVPVTLKMRTGWCQQHRNAVQLARAFEGVGIQMLTVHGRTREQGY
HHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCC
KGHAEYDTIAAVKAAVRVPVVANGDIDSPEKAKAVLAATGADAIMIGRAAQGRPWIFREI
CCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHEEEEECCCCEEEEECCCCCCCHHHHHH
GHYLATGEHLAPPLVAEVRRLLLDHLQDHYALYGEATGVRSARKHIAWYVRALPGGEAFR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHH
QHINTIDDCAAQWQAVALFFEELGRHMDRLPAAPPEGAGTDTDDTQEPQGLPA
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]