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The map label for this gene is thiC

Identifier: 120609523

GI number: 120609523

Start: 898143

End: 899996

Strand: Direct

Name: thiC

Synonym: Aave_0829

Alternate gene names: 120609523

Gene position: 898143-899996 (Clockwise)

Preceding gene: 120609522

Following gene: 120609525

Centisome position: 16.78

GC content: 67.96

Gene sequence:

>1854_bases
ATGAACGCCCCCGACAAGTTCGCCCAGCTGCTCGCGCTGACCCGCGAACCCTTCCCCGCCTCCACCAAGAACTACCTCAT
CGGCAGCCGCCCCGACGTGCGCGTGCCCGTGCGAGATATCGCGCTCACCAACGGCGAGCAGGTCAGCGTGTACGACACCT
CAGGCCCCTACACCGACCCGGCTGCCGAGATCGACGTGCGCCGGGGCCTGCCCAGCGTGCGTGGCGCGTGGATCGAGGGC
CGGGGCGACACCGAGCGCTACGAAGGCCGCCAGCGGGTGGCGCTGGACGACGGCAGCAAGTCGGAAGACGCCGCCCGCCT
GGCCCAGCTGCGCGCCGAGGCCGCCGCGCTGCAGCGCGCGCCCCTGCGCGCGAAGAGCGGCGCCAGCCACACCGGCAATG
TCACGCAGATGCACTACGCGAAGAAGGGCATCGTCACGCCCGAGATGGAGTACGTGGCCCTGCGCGAGAACGGCCGCCGC
GAATGGATGGCGCAGTATCAGCAGGACGCGGCGCGCGAGCAGCGCCTGGCCGGCAACCCGATGGGCGCGAGCATCCCGAA
GATCATCACGCCCGAATTCGTGCGCGACGAAGTGGCGCGGGGCCGGGCCATCATCCCGGCCAACATCAACCACCCCGAGG
TCGAGCCCATGGCCATCGGGCGCACCTTCAAGGTGAAGATCAACGCAAACATCGGCAATTCGGCCGTCACCTCCAGCATC
GAGGAAGAGGTGGAAAAGCTGGTCTGGGCGATCCGCTGGGGCGCGGACAACGTGATGGACCTCTCCACCGGCAAGAACAT
CCACACCACGCGCGACTGGATCGTGCGCAACAGCCCCGTGCCCATCGGCACCGTGCCCATCTACCAGGCGCTGGAGAAGG
TGGGGGGCATCGCCGAGGACCTGACCTGGGAGATCTTCCGCGACACGCTCGTCGAGCAGGCCGAGCAGGGCGTGGACTAC
TTCACCATCCATGCGGGCGTGCGGCTGGCCTTCATCCACCTGACTGCGCAGCGGCGCACGGGCATCGTCTCGCGCGGCGG
CTCCATCATGGCCAAGTGGTGCATGGCCCACCACCGCGAGAGCTTCCTCTACGAGCACTTCGAGGACATCTGCGACATCA
TGAAGCAGTACGACGTCAGTTTCAGCCTGGGCGACGGCCTGCGGCCCGGCTGCGCGTCCGATGCCAACGACGAGGCGCAG
TTCGCCGAACTGCGCACGCTGGGCGAATTGACGCGGACCGCCTGGAAGCACGACGTGCAGACCATGATCGAGGGCCCCGG
CCACGTGCCCATGCACATGATCCAGGCCAACATGACCGAGCAGCTCAGGACCTGCCATGAAGCACCGTTCTACACGCTCG
GGCCGCTGACCATCGACATCGCCCCCGGCTACGACCACATCGCCAGCGCCATCGGCGCGGCCATGATCGGCTGGATGGGC
ACGGCCATGCTCTGCTACGTGACGCCCAAGGAGCACCTGGGCCTGCCCGACCGCGACGACGTGAAGCAGGGGATCATCGC
GTACAAGATCGCGGCCCATGCGGCCGACGTGGCCAAGGGCCATCCGGGGGCCCGCGCGCGCGACGACGCGCTCAGCCAGG
CGCGCTTCGACTTCCGCTGGCAGGACCAGTTCAACCTGGGCCTGGACCCGGAAACCGCGCAGCAGTACCACGACGAGACC
CTGCCCAAGGATTCGGCCAAGGTGGCGCACTTCTGCTCGATGTGCGGCCCGAAGTTCTGCTCGATGAAGATCACGCAGGA
AGTGCGCGAATTCGCGGCGCAGGGCATGCAGGAGAAAGCCCGGGAGTTCCGCGGCACGGGCGGGGAACTGTACGTGCCGA
TCCAGCCGGCATGA

Upstream 100 bases:

>100_bases
TGGGGAAGGTGCGCGCAGCGGCGCCTCCATCCGGGGGCCGGTACGGCGCACCTGGCCCCATGCGGAGCACATTTCCATCC
ACCCGAGGAGATTGCCCGCC

Downstream 100 bases:

>100_bases
CGCCGCCCGCCCGGGCCGGGCGGGAGCGGTTCAGCCCGCGGTGGCCAGCAGGCGCTGCGGTCGCGGAGGCGGCAGCGCGC
TGCGTGCCGGCGGCGCGGCA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MNAPDKFAQLLALTREPFPASTKNYLIGSRPDVRVPVRDIALTNGEQVSVYDTSGPYTDPAAEIDVRRGLPSVRGAWIEG
RGDTERYEGRQRVALDDGSKSEDAARLAQLRAEAAALQRAPLRAKSGASHTGNVTQMHYAKKGIVTPEMEYVALRENGRR
EWMAQYQQDAAREQRLAGNPMGASIPKIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRTFKVKINANIGNSAVTSSI
EEEVEKLVWAIRWGADNVMDLSTGKNIHTTRDWIVRNSPVPIGTVPIYQALEKVGGIAEDLTWEIFRDTLVEQAEQGVDY
FTIHAGVRLAFIHLTAQRRTGIVSRGGSIMAKWCMAHHRESFLYEHFEDICDIMKQYDVSFSLGDGLRPGCASDANDEAQ
FAELRTLGELTRTAWKHDVQTMIEGPGHVPMHMIQANMTEQLRTCHEAPFYTLGPLTIDIAPGYDHIASAIGAAMIGWMG
TAMLCYVTPKEHLGLPDRDDVKQGIIAYKIAAHAADVAKGHPGARARDDALSQARFDFRWQDQFNLGLDPETAQQYHDET
LPKDSAKVAHFCSMCGPKFCSMKITQEVREFAAQGMQEKAREFRGTGGELYVPIQPA

Sequences:

>Translated_617_residues
MNAPDKFAQLLALTREPFPASTKNYLIGSRPDVRVPVRDIALTNGEQVSVYDTSGPYTDPAAEIDVRRGLPSVRGAWIEG
RGDTERYEGRQRVALDDGSKSEDAARLAQLRAEAAALQRAPLRAKSGASHTGNVTQMHYAKKGIVTPEMEYVALRENGRR
EWMAQYQQDAAREQRLAGNPMGASIPKIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRTFKVKINANIGNSAVTSSI
EEEVEKLVWAIRWGADNVMDLSTGKNIHTTRDWIVRNSPVPIGTVPIYQALEKVGGIAEDLTWEIFRDTLVEQAEQGVDY
FTIHAGVRLAFIHLTAQRRTGIVSRGGSIMAKWCMAHHRESFLYEHFEDICDIMKQYDVSFSLGDGLRPGCASDANDEAQ
FAELRTLGELTRTAWKHDVQTMIEGPGHVPMHMIQANMTEQLRTCHEAPFYTLGPLTIDIAPGYDHIASAIGAAMIGWMG
TAMLCYVTPKEHLGLPDRDDVKQGIIAYKIAAHAADVAKGHPGARARDDALSQARFDFRWQDQFNLGLDPETAQQYHDET
LPKDSAKVAHFCSMCGPKFCSMKITQEVREFAAQGMQEKAREFRGTGGELYVPIQPA
>Mature_617_residues
MNAPDKFAQLLALTREPFPASTKNYLIGSRPDVRVPVRDIALTNGEQVSVYDTSGPYTDPAAEIDVRRGLPSVRGAWIEG
RGDTERYEGRQRVALDDGSKSEDAARLAQLRAEAAALQRAPLRAKSGASHTGNVTQMHYAKKGIVTPEMEYVALRENGRR
EWMAQYQQDAAREQRLAGNPMGASIPKIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRTFKVKINANIGNSAVTSSI
EEEVEKLVWAIRWGADNVMDLSTGKNIHTTRDWIVRNSPVPIGTVPIYQALEKVGGIAEDLTWEIFRDTLVEQAEQGVDY
FTIHAGVRLAFIHLTAQRRTGIVSRGGSIMAKWCMAHHRESFLYEHFEDICDIMKQYDVSFSLGDGLRPGCASDANDEAQ
FAELRTLGELTRTAWKHDVQTMIEGPGHVPMHMIQANMTEQLRTCHEAPFYTLGPLTIDIAPGYDHIASAIGAAMIGWMG
TAMLCYVTPKEHLGLPDRDDVKQGIIAYKIAAHAADVAKGHPGARARDDALSQARFDFRWQDQFNLGLDPETAQQYHDET
LPKDSAKVAHFCSMCGPKFCSMKITQEVREFAAQGMQEKAREFRGTGGELYVPIQPA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=620, Percent_Identity=65.4838709677419, Blast_Score=824, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_ACIAC (A1TKD9)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969201.1
- STRING:   A1TKD9
- GeneID:   4668142
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_0829
- NMPDR:   fig|397945.5.peg.720
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   A1TKD9
- ProtClustDB:   PRK09284
- BioCyc:   AAVE397945:AAVE_0829-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 68394; Mature: 68394

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: BINDING 230-230 BINDING 259-259 BINDING 288-288 BINDING 324-324 BINDING 424-424 BINDING 451-451

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPDKFAQLLALTREPFPASTKNYLIGSRPDVRVPVRDIALTNGEQVSVYDTSGPYTDP
CCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHEEECCCCEEEEEECCCCCCCC
AAEIDVRRGLPSVRGAWIEGRGDTERYEGRQRVALDDGSKSEDAARLAQLRAEAAALQRA
HHHHHHHHCCCHHCCCEEECCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHC
PLRAKSGASHTGNVTQMHYAKKGIVTPEMEYVALRENGRREWMAQYQQDAAREQRLAGNP
CCCCCCCCCCCCCCCEEHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
MGASIPKIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRTFKVKINANIGNSAVTSSI
CCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECEEEEEEEECCCCCHHHHHHH
EEEVEKLVWAIRWGADNVMDLSTGKNIHTTRDWIVRNSPVPIGTVPIYQALEKVGGIAED
HHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHCCHHHH
LTWEIFRDTLVEQAEQGVDYFTIHAGVRLAFIHLTAQRRTGIVSRGGSIMAKWCMAHHRE
HHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEHHHHCCCHHCCCHHHHHHHHHHHHH
SFLYEHFEDICDIMKQYDVSFSLGDGLRPGCASDANDEAQFAELRTLGELTRTAWKHDVQ
HHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TMIEGPGHVPMHMIQANMTEQLRTCHEAPFYTLGPLTIDIAPGYDHIASAIGAAMIGWMG
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCHHHHHHHHHHHHHHHHH
TAMLCYVTPKEHLGLPDRDDVKQGIIAYKIAAHAADVAKGHPGARARDDALSQARFDFRW
HEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEE
QDQFNLGLDPETAQQYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREFAAQGMQEKA
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
REFRGTGGELYVPIQPA
HHHCCCCCEEEEEECCC
>Mature Secondary Structure
MNAPDKFAQLLALTREPFPASTKNYLIGSRPDVRVPVRDIALTNGEQVSVYDTSGPYTDP
CCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHEEECCCCEEEEEECCCCCCCC
AAEIDVRRGLPSVRGAWIEGRGDTERYEGRQRVALDDGSKSEDAARLAQLRAEAAALQRA
HHHHHHHHCCCHHCCCEEECCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHC
PLRAKSGASHTGNVTQMHYAKKGIVTPEMEYVALRENGRREWMAQYQQDAAREQRLAGNP
CCCCCCCCCCCCCCCEEHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
MGASIPKIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRTFKVKINANIGNSAVTSSI
CCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECEEEEEEEECCCCCHHHHHHH
EEEVEKLVWAIRWGADNVMDLSTGKNIHTTRDWIVRNSPVPIGTVPIYQALEKVGGIAED
HHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHCCHHHH
LTWEIFRDTLVEQAEQGVDYFTIHAGVRLAFIHLTAQRRTGIVSRGGSIMAKWCMAHHRE
HHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEHHHHCCCHHCCCHHHHHHHHHHHHH
SFLYEHFEDICDIMKQYDVSFSLGDGLRPGCASDANDEAQFAELRTLGELTRTAWKHDVQ
HHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TMIEGPGHVPMHMIQANMTEQLRTCHEAPFYTLGPLTIDIAPGYDHIASAIGAAMIGWMG
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCHHHHHHHHHHHHHHHHH
TAMLCYVTPKEHLGLPDRDDVKQGIIAYKIAAHAADVAKGHPGARARDDALSQARFDFRW
HEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEE
QDQFNLGLDPETAQQYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREFAAQGMQEKA
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
REFRGTGGELYVPIQPA
HHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA