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The map label for this gene is ddl

Identifier: 120609517

GI number: 120609517

Start: 891866

End: 892870

Strand: Direct

Name: ddl

Synonym: Aave_0823

Alternate gene names: 120609517

Gene position: 891866-892870 (Clockwise)

Preceding gene: 120609516

Following gene: 120609518

Centisome position: 16.66

GC content: 69.55

Gene sequence:

>1005_bases
ATGGCCGCGGGCTTACCTGAAACCGAAATCGACGTGCGGGCGCTGGGCAAGGTGGCCGTGCTGATGGGCGGCGACTCCGC
CGAGCGGGAGGTTTCGCTCATGTCCGGCGGCGGCGTGCTGCAGGCGCTGCGTGCGCGCGGCGTGGACGCGCATGCCTTCG
ATCCGTCCCAGTCCGACCTGTCGGAGCTCAAGAGCCACGGCTACGCGCGCTGCTTCATCGCACTGCACGGCCGGCATGGC
GAGGACGGCACCGTGCAGGGCGCGCTGGAGTTGCTGGGCATTCCCTATACCGGACCGGGCGTGATGGCGTCGGCCATCGC
CATGGACAAGATCATGACCAAGCGCATCTGGCGCTTCGAGGGCCTGCCCACGCCGGACTGGCGGCTGGTGTCGAGTGCTG
CCGAGACCGCGCAGGCGCTGGAGGACCTGGGCTCGCCCATGATCGTCAAGCCCTCGCGCGAAGGCTCCACCATCGGACTG
ACCAAGGTCACCTCGCCCGGACAGTGCGAGCAGGCCTACCGCCTGGCCTCGCGCTACGACCCCGAGGTGCTGTGCGAGCA
ATTCATCGAGGGCGAGGAAACCACCTGCCCCGTGCTGGGCCAGGGCGCGGGCGCGCATGCGCTGCCGGTGATCCGTATCG
TGGCGCCCGAGGGCAACTACGACTACCAGAACAAGTACTTCACCGACGTCACGCAGTACCACTGCCCCTGCGGCCTGCCC
GAGCAGGAGGAGCGCGAGATCCAGCGCCTGGTGGTGGAGGCGTTCCGCACGCTGCAGTGCCGCGGCTGGGCCCGCGCCGA
CATCATGATCCGCGCGAGCGACCGCAAGCCCTTCCTGCTGGAGATCAACACCTCCCCCGGCATGACCGGCCATTCGCTGG
TACCCATGTCGGCGCGCGCCATGGGCGTGAGCTACGAGGAACTGTGCCTGCGCATCCTGGCGGGGGCGTCGCTCGACGCG
CGCCAGGGCCAGGTGGACGCCGCAGGGCATCCGGGAGCCGCATGA

Upstream 100 bases:

>100_bases
ACGTGGTGCTGTGCATGGGGGCCGGCTCGATCGGCGCCGTGCCCGCGAAGGTGGTGGAGATGCTGCGCACGGACGTGCCG
GCGATGCAGGAGGATCGGTG

Downstream 100 bases:

>100_bases
CCACGAACACGCTGCCCGCACCGCTGGACGTGAAGCTCATGAACTGGACCGCGACGGTCCTGTTCGCGGGCTGCGCCGTG
GCCGTGCTGGTGGCGGCCGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAAGLPETEIDVRALGKVAVLMGGDSAEREVSLMSGGGVLQALRARGVDAHAFDPSQSDLSELKSHGYARCFIALHGRHG
EDGTVQGALELLGIPYTGPGVMASAIAMDKIMTKRIWRFEGLPTPDWRLVSSAAETAQALEDLGSPMIVKPSREGSTIGL
TKVTSPGQCEQAYRLASRYDPEVLCEQFIEGEETTCPVLGQGAGAHALPVIRIVAPEGNYDYQNKYFTDVTQYHCPCGLP
EQEEREIQRLVVEAFRTLQCRGWARADIMIRASDRKPFLLEINTSPGMTGHSLVPMSARAMGVSYEELCLRILAGASLDA
RQGQVDAAGHPGAA

Sequences:

>Translated_334_residues
MAAGLPETEIDVRALGKVAVLMGGDSAEREVSLMSGGGVLQALRARGVDAHAFDPSQSDLSELKSHGYARCFIALHGRHG
EDGTVQGALELLGIPYTGPGVMASAIAMDKIMTKRIWRFEGLPTPDWRLVSSAAETAQALEDLGSPMIVKPSREGSTIGL
TKVTSPGQCEQAYRLASRYDPEVLCEQFIEGEETTCPVLGQGAGAHALPVIRIVAPEGNYDYQNKYFTDVTQYHCPCGLP
EQEEREIQRLVVEAFRTLQCRGWARADIMIRASDRKPFLLEINTSPGMTGHSLVPMSARAMGVSYEELCLRILAGASLDA
RQGQVDAAGHPGAA
>Mature_333_residues
AAGLPETEIDVRALGKVAVLMGGDSAEREVSLMSGGGVLQALRARGVDAHAFDPSQSDLSELKSHGYARCFIALHGRHGE
DGTVQGALELLGIPYTGPGVMASAIAMDKIMTKRIWRFEGLPTPDWRLVSSAAETAQALEDLGSPMIVKPSREGSTIGLT
KVTSPGQCEQAYRLASRYDPEVLCEQFIEGEETTCPVLGQGAGAHALPVIRIVAPEGNYDYQNKYFTDVTQYHCPCGLPE
QEEREIQRLVVEAFRTLQCRGWARADIMIRASDRKPFLLEINTSPGMTGHSLVPMSARAMGVSYEELCLRILAGASLDAR
QGQVDAAGHPGAA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=305, Percent_Identity=51.4754098360656, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI1786579, Length=345, Percent_Identity=31.8840579710145, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_ACIAC (A1TKD3)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969195.1
- ProteinModelPortal:   A1TKD3
- SMR:   A1TKD3
- STRING:   A1TKD3
- GeneID:   4668635
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_0823
- NMPDR:   fig|397945.5.peg.714
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   NLSNALE
- PhylomeDB:   A1TKD3
- ProtClustDB:   PRK01372
- BioCyc:   AAVE397945:AAVE_0823-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 35911; Mature: 35780

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGLPETEIDVRALGKVAVLMGGDSAEREVSLMSGGGVLQALRARGVDAHAFDPSQSDL
CCCCCCCCCHHHHHHCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCHHHH
SELKSHGYARCFIALHGRHGEDGTVQGALELLGIPYTGPGVMASAIAMDKIMTKRIWRFE
HHHHHCCCEEEEEEEECCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GLPTPDWRLVSSAAETAQALEDLGSPMIVKPSREGSTIGLTKVTSPGQCEQAYRLASRYD
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCC
PEVLCEQFIEGEETTCPVLGQGAGAHALPVIRIVAPEGNYDYQNKYFTDVTQYHCPCGLP
HHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCC
EQEEREIQRLVVEAFRTLQCRGWARADIMIRASDRKPFLLEINTSPGMTGHSLVPMSARA
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCEECCCCHHH
MGVSYEELCLRILAGASLDARQGQVDAAGHPGAA
HCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAGLPETEIDVRALGKVAVLMGGDSAEREVSLMSGGGVLQALRARGVDAHAFDPSQSDL
CCCCCCCCHHHHHHCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCHHHH
SELKSHGYARCFIALHGRHGEDGTVQGALELLGIPYTGPGVMASAIAMDKIMTKRIWRFE
HHHHHCCCEEEEEEEECCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GLPTPDWRLVSSAAETAQALEDLGSPMIVKPSREGSTIGLTKVTSPGQCEQAYRLASRYD
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCC
PEVLCEQFIEGEETTCPVLGQGAGAHALPVIRIVAPEGNYDYQNKYFTDVTQYHCPCGLP
HHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCC
EQEEREIQRLVVEAFRTLQCRGWARADIMIRASDRKPFLLEINTSPGMTGHSLVPMSARA
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCEECCCCHHH
MGVSYEELCLRILAGASLDARQGQVDAAGHPGAA
HCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA