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The map label for this gene is murD [H]

Identifier: 120609513

GI number: 120609513

Start: 886012

End: 888015

Strand: Direct

Name: murD [H]

Synonym: Aave_0819

Alternate gene names: 120609513

Gene position: 886012-888015 (Clockwise)

Preceding gene: 120609512

Following gene: 120609514

Centisome position: 16.55

GC content: 74.4

Gene sequence:

>2004_bases
ATGAACCCGACCGATCGCGACCTCCATCCCGCCACCGGCGCCCTCCTGGACGCCGGTGCCGCGCCATGGCCGCTCCCTGC
CGCCGTGGCGCAGGAGGCGCCCGCGCCGGCCGAAAGCCTGGAAGCCGGGAGCGCGGCAGCTGAAAGCCCGGCCGGGGAGG
GCGCGCCAGCGCACGCCACCGAAGCCGCCGGGGCGCCGGCTGCCGTGGAGGCGCAAAGCAGTCCGGAGCCGGTGGCAGAG
CCCACCGAGCAGCCAGCGGCGCTGGCGGACGGTTTCGACATGACCGCGCCGCGCGTTTCCGCCGCGCAGGAGGCCGCGGA
GTTCGTGGCCCGCATCTTCGCGGACGTCCAGGCCGAGGCATCCCCGGCAGCCGGCGCCGCCGATGCGGGCGAGGCCCCCG
TGGGCGAGGCTGTCGGCGCGCAGGCCCCGGCGGATGGAGACGAGCCCGCTGAACCCCTGGGCCGGCCGGCGGACACGCTG
CGCCCCCTGGCGGGCCGCCGCATCCTCATCCTGGGACTGGGCGCCTCGGGCCTGGCCATGGCGCGCTGGTGCGTGCGCTG
CGGCGCCGAGGACGTGACCGTGGCCGATACCCGCGAGGCCCCGCCCCAATTGGCGGTGCTGCAGTCCGAACTGCCCGGCG
TGCGCTTCGTTCCAGGAGCGTTCGACGCGGCGCTGGTGCAGGGCCACGGCCTGCATGCCGTCTATCGCTCGCCGGGCCTG
GCGCCCGAGGCGGTGGCACCCGTGTTCGTCGCGGCCGCCGCCGAAGGCATCGTCACGGGTGGCGAGCTCGGCCTTTTCGC
CATGGCGCTGGATGCGCTCAAGGCCTCCCATGGCTACGCGCCCGCGGTGCTGGCCATCACCGGCACCAACGGCAAGACCA
CGGTGACCTCGCTCACGGGCCTGCTCGTCGAATGCGCGGGCCGCTCCGTGGCCGTGGCCGGCAACATCGGCCCGACCCTG
CTCGACACGCTGGGCCGCCACCTGGACGCCGACACCCTGCCGCAGGCCTGGGTGCTGGAACTCTCCAGTTTCCAGCTCGA
AGGGGCGGCCGGCTTCGAGCCCACCGCTGCGACCGTGCTCAACGTCACGCAGGACCACCTCGACTGGCACGGCGACATGC
AGGCCTATGCGCGGGCCAAGGCCCGGATCTTCGGCGAATCCGGCCTCATGGTGCTCAACCGCGAGGACGCCGCCGTGATG
GGCATGCTGCCGCCGCCCGTGCGCGTGAAGCTGCAGAAGCCGCAATTGCGCGCCCATGTGACGTTCGGGGCGGACATGCC
CCAGCGTCCGGGGGACTTCGGCATCGAGACCGTGAACGGCATGGCCTGGCTGGTGCGCGCGCAGGAGTCCGACGAGACGG
CGAGGCGCACGCGCAGCCACGCCGAGGAAGATCTGCACATCCAGCGCCTGATACCGGCCGACGCGCTGCGCATCCGCGGC
CGCCACAACGCCGTGAACGCGCTGGCCGCGCTGGCGCTCGCGCACGCGGCCGGCTGCGCGTTCGCGCCCATGCTCTACGC
CCTGCGCGAGTACCGCGGCGAGCCGCACCGCGTGGAGCCCGTGGGCCGGGTGAACGAGGTGGAGTATTTCGACGACAGCA
AGGGCACCAACGTGGGCGCCACGGTGGCCGCGCTGGGCGGCCTGGGTGCCGACCGCCGCGTCGTGGTGATCCTGGGCGGC
GACGGCAAGGGCCAGGACTTCTCGCCGCTGGCCATGCCCGTGTCGCGCTATGTGCGCGCCGCCGTGCTCATCGGCCGCGA
CGGGCCGCAGATCCGCGCGGCCCTGCGGGACGCGGGCGTGCCGCTCATCGACGCGCCCACGCTGCCGGAGGCCGTGCGCC
TGGCCGCGCAGCGGGCCCATGCGGGCGACGCGGTGCTGATGTCGCCCGCCTGCGCGAGCTTCGACATGTTCCGCAACTAC
GAACACCGCGCCGAGGTGTTCCGCGAGGCCGTGCAGGCGCTCGCGGACGAGGCCGGACAGGCGCTGGAAGGGGGGCTGGC
ATGA

Upstream 100 bases:

>100_bases
ATTTCGAGAAGAGCGGCTGGAAAGAGACGCAGGTCGTCGTGCGTTTCTGGATCATCACCATGCTGCTGTGCCTGATCGGC
CTCACCACGCTCAAGCTGCG

Downstream 100 bases:

>100_bases
ACGCGGGCGCTGCTGCATCTTCGTCGCGAGGCCTGCGCCACCGGATCGCCGGCTGGTTCGGGCGCGGCGCCTCGCCCGCG
GCCGACGTGCTGCCCGTGCG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MNPTDRDLHPATGALLDAGAAPWPLPAAVAQEAPAPAESLEAGSAAAESPAGEGAPAHATEAAGAPAAVEAQSSPEPVAE
PTEQPAALADGFDMTAPRVSAAQEAAEFVARIFADVQAEASPAAGAADAGEAPVGEAVGAQAPADGDEPAEPLGRPADTL
RPLAGRRILILGLGASGLAMARWCVRCGAEDVTVADTREAPPQLAVLQSELPGVRFVPGAFDAALVQGHGLHAVYRSPGL
APEAVAPVFVAAAAEGIVTGGELGLFAMALDALKASHGYAPAVLAITGTNGKTTVTSLTGLLVECAGRSVAVAGNIGPTL
LDTLGRHLDADTLPQAWVLELSSFQLEGAAGFEPTAATVLNVTQDHLDWHGDMQAYARAKARIFGESGLMVLNREDAAVM
GMLPPPVRVKLQKPQLRAHVTFGADMPQRPGDFGIETVNGMAWLVRAQESDETARRTRSHAEEDLHIQRLIPADALRIRG
RHNAVNALAALALAHAAGCAFAPMLYALREYRGEPHRVEPVGRVNEVEYFDDSKGTNVGATVAALGGLGADRRVVVILGG
DGKGQDFSPLAMPVSRYVRAAVLIGRDGPQIRAALRDAGVPLIDAPTLPEAVRLAAQRAHAGDAVLMSPACASFDMFRNY
EHRAEVFREAVQALADEAGQALEGGLA

Sequences:

>Translated_667_residues
MNPTDRDLHPATGALLDAGAAPWPLPAAVAQEAPAPAESLEAGSAAAESPAGEGAPAHATEAAGAPAAVEAQSSPEPVAE
PTEQPAALADGFDMTAPRVSAAQEAAEFVARIFADVQAEASPAAGAADAGEAPVGEAVGAQAPADGDEPAEPLGRPADTL
RPLAGRRILILGLGASGLAMARWCVRCGAEDVTVADTREAPPQLAVLQSELPGVRFVPGAFDAALVQGHGLHAVYRSPGL
APEAVAPVFVAAAAEGIVTGGELGLFAMALDALKASHGYAPAVLAITGTNGKTTVTSLTGLLVECAGRSVAVAGNIGPTL
LDTLGRHLDADTLPQAWVLELSSFQLEGAAGFEPTAATVLNVTQDHLDWHGDMQAYARAKARIFGESGLMVLNREDAAVM
GMLPPPVRVKLQKPQLRAHVTFGADMPQRPGDFGIETVNGMAWLVRAQESDETARRTRSHAEEDLHIQRLIPADALRIRG
RHNAVNALAALALAHAAGCAFAPMLYALREYRGEPHRVEPVGRVNEVEYFDDSKGTNVGATVAALGGLGADRRVVVILGG
DGKGQDFSPLAMPVSRYVRAAVLIGRDGPQIRAALRDAGVPLIDAPTLPEAVRLAAQRAHAGDAVLMSPACASFDMFRNY
EHRAEVFREAVQALADEAGQALEGGLA
>Mature_667_residues
MNPTDRDLHPATGALLDAGAAPWPLPAAVAQEAPAPAESLEAGSAAAESPAGEGAPAHATEAAGAPAAVEAQSSPEPVAE
PTEQPAALADGFDMTAPRVSAAQEAAEFVARIFADVQAEASPAAGAADAGEAPVGEAVGAQAPADGDEPAEPLGRPADTL
RPLAGRRILILGLGASGLAMARWCVRCGAEDVTVADTREAPPQLAVLQSELPGVRFVPGAFDAALVQGHGLHAVYRSPGL
APEAVAPVFVAAAAEGIVTGGELGLFAMALDALKASHGYAPAVLAITGTNGKTTVTSLTGLLVECAGRSVAVAGNIGPTL
LDTLGRHLDADTLPQAWVLELSSFQLEGAAGFEPTAATVLNVTQDHLDWHGDMQAYARAKARIFGESGLMVLNREDAAVM
GMLPPPVRVKLQKPQLRAHVTFGADMPQRPGDFGIETVNGMAWLVRAQESDETARRTRSHAEEDLHIQRLIPADALRIRG
RHNAVNALAALALAHAAGCAFAPMLYALREYRGEPHRVEPVGRVNEVEYFDDSKGTNVGATVAALGGLGADRRVVVILGG
DGKGQDFSPLAMPVSRYVRAAVLIGRDGPQIRAALRDAGVPLIDAPTLPEAVRLAAQRAHAGDAVLMSPACASFDMFRNY
EHRAEVFREAVQALADEAGQALEGGLA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=489, Percent_Identity=38.8548057259714, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 68749; Mature: 68749

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTDRDLHPATGALLDAGAAPWPLPAAVAQEAPAPAESLEAGSAAAESPAGEGAPAHAT
CCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCH
EAAGAPAAVEAQSSPEPVAEPTEQPAALADGFDMTAPRVSAAQEAAEFVARIFADVQAEA
HCCCCCCEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SPAAGAADAGEAPVGEAVGAQAPADGDEPAEPLGRPADTLRPLAGRRILILGLGASGLAM
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCHHHHHHCCCCEEEEEECCCCHHHH
ARWCVRCGAEDVTVADTREAPPQLAVLQSELPGVRFVPGAFDAALVQGHGLHAVYRSPGL
HHHHHHCCCCCCEEECCCCCCCHHHHHHHCCCCCEECCCCHHHHHHCCCCCEEEECCCCC
APEAVAPVFVAAAAEGIVTGGELGLFAMALDALKASHGYAPAVLAITGTNGKTTVTSLTG
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
LLVECAGRSVAVAGNIGPTLLDTLGRHLDADTLPQAWVLELSSFQLEGAAGFEPTAATVL
HHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCCCCCHHHHH
NVTQDHLDWHGDMQAYARAKARIFGESGLMVLNREDAAVMGMLPPPVRVKLQKPQLRAHV
HHHHHHHCCCCCHHHHHHHHHHEECCCCEEEEECCCCEEEECCCCCCEEEEECCCEEEEE
TFGADMPQRPGDFGIETVNGMAWLVRAQESDETARRTRSHAEEDLHIQRLIPADALRIRG
EECCCCCCCCCCCCCEECCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHCCHHHHHCCC
RHNAVNALAALALAHAAGCAFAPMLYALREYRGEPHRVEPVGRVNEVEYFDDSKGTNVGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCH
TVAALGGLGADRRVVVILGGDGKGQDFSPLAMPVSRYVRAAVLIGRDGPQIRAALRDAGV
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCC
PLIDAPTLPEAVRLAAQRAHAGDAVLMSPACASFDMFRNYEHRAEVFREAVQALADEAGQ
CEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
ALEGGLA
HHCCCCC
>Mature Secondary Structure
MNPTDRDLHPATGALLDAGAAPWPLPAAVAQEAPAPAESLEAGSAAAESPAGEGAPAHAT
CCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCH
EAAGAPAAVEAQSSPEPVAEPTEQPAALADGFDMTAPRVSAAQEAAEFVARIFADVQAEA
HCCCCCCEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SPAAGAADAGEAPVGEAVGAQAPADGDEPAEPLGRPADTLRPLAGRRILILGLGASGLAM
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCHHHHHHCCCCEEEEEECCCCHHHH
ARWCVRCGAEDVTVADTREAPPQLAVLQSELPGVRFVPGAFDAALVQGHGLHAVYRSPGL
HHHHHHCCCCCCEEECCCCCCCHHHHHHHCCCCCEECCCCHHHHHHCCCCCEEEECCCCC
APEAVAPVFVAAAAEGIVTGGELGLFAMALDALKASHGYAPAVLAITGTNGKTTVTSLTG
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
LLVECAGRSVAVAGNIGPTLLDTLGRHLDADTLPQAWVLELSSFQLEGAAGFEPTAATVL
HHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCCCCCHHHHH
NVTQDHLDWHGDMQAYARAKARIFGESGLMVLNREDAAVMGMLPPPVRVKLQKPQLRAHV
HHHHHHHCCCCCHHHHHHHHHHEECCCCEEEEECCCCEEEECCCCCCEEEEECCCEEEEE
TFGADMPQRPGDFGIETVNGMAWLVRAQESDETARRTRSHAEEDLHIQRLIPADALRIRG
EECCCCCCCCCCCCCEECCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHCCHHHHHCCC
RHNAVNALAALALAHAAGCAFAPMLYALREYRGEPHRVEPVGRVNEVEYFDDSKGTNVGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCH
TVAALGGLGADRRVVVILGGDGKGQDFSPLAMPVSRYVRAAVLIGRDGPQIRAALRDAGV
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCC
PLIDAPTLPEAVRLAAQRAHAGDAVLMSPACASFDMFRNYEHRAEVFREAVQALADEAGQ
CEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
ALEGGLA
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA