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The map label for this gene is ubiH [H]

Identifier: 120597559

GI number: 120597559

Start: 806165

End: 807403

Strand: Direct

Name: ubiH [H]

Synonym: Sputw3181_0728

Alternate gene names: 120597559

Gene position: 806165-807403 (Clockwise)

Preceding gene: 120597558

Following gene: 120597560

Centisome position: 17.12

GC content: 51.65

Gene sequence:

>1239_bases
ATGACGGTAACGAATCACATGGATACTTCAGTTCAAAACGTCGACATCGCGATTGTAGGCGGTGCCATGGCGGGGGCAAT
CTTGGCGTTGGCACTCGCGCGCCTTTCCCACACAACAGCCCCACTTTCTGTTGCCTTAGTTGAGGCACACCTTCCCGATA
AGCAACACCCCGGATTCGATGCCCGCTCTATTGCCATTGCCCATGGTTCTGTCTTTGAATTATCCCGCTTAGGTTTATGG
CAAAAGCTCGCCCATTTGGGCACGGCGATTGAAAATATCCATGTGTCGGATCGCGGCCATTTTGGTATGACAGAGTTAAA
TGCGGCACAGTTTCATTTAGATGCCCTAGGCCAAGTAGTCGAGCTTGAACGTGTCGGCCAAGTGCTGTTTAACGAGTTAC
ATAAGAGCGAGGTTAAACTTTACTGCCCCGCTAAGCTTGACCAAGTAGAAGCCGCAGTCGAAGGCCACACCTTAGTGCTT
GATACGGGCGAGCGCATTCACGCCAAACTTGTGGTCGCCGCCGATGGTTTACAATCTAAAGTGCGCAGCAGTTTTAATCT
GCCCATTACCCAAGTGGCGTTCGAGCAAACTGCGATTATTGCCAATATTCAAACCGCTGATTTGCCAACCAATAGGCCTC
AAAAACATTGGGCCTACGAGCGTTTTACCGAGACAGGTCCCTTAGCACTGCTACCTATGGGCGATAGCCAAGGCCAACCG
CGTTTATCCCTCGTATGGGCCCTCGATAATGCGCGGGCTAAGCAGATGCAAACCGTGCCTAAGGCCGAATTTTTAGCGGC
GCTGCAACAGGCCTTTGGTTATCGTGCGGGCACGTTTATCGATGTTAGCGCGCGCCATGCGTATCCGCTTAATTTGTCTT
ATATGCCAAGGCCAATCCACCATAGATGTGTGTTTATCGGCAATGCGGCGCAGACGCTGCATCCGATTGCAGGGCAAGGC
TTTAACTTAGGGCTAAGGGATGTAGTCAGCTTGCTTGAGGTGGTCAAATCGGCGCTAGTACAGGGGGAGGATATCGGCTC
TGCCGCTGTGACCCATGCGTACCTTCATGCCCGCGAGGACGACAGAAACACCACTATCCGTAATATCGAATTTTTAGTTC
GTGGCTTCTCCAACCAATACTGGCCCTTGGCCCTTGGGCGTAATTTAGGATTACGGCTCTTGTCTTGGTTTCCGCCACTC
AAAACCCCCGTCGCTCAAAAAGCGATGGGTTGGAAATAA

Upstream 100 bases:

>100_bases
ATGTAAAAAGGTTGATACGCTTCCCCAACATTTGCAGATTCAGCCTTTTAGTACATGGTTCTACTTTTTAGGATCATGCC
ATAGCTTGAATGAGATCCCC

Downstream 100 bases:

>100_bases
CACAGCAATTTACTGGTATCGCAACGCGAAAAGGATGTACCCATGTTAAGTTCACAAGCCTATGACATTGCCATTGTGGG
TGGCGGCATGGTGGGACTCG

Product: 2-polyprenyl-6-methoxyphenol 4-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MTVTNHMDTSVQNVDIAIVGGAMAGAILALALARLSHTTAPLSVALVEAHLPDKQHPGFDARSIAIAHGSVFELSRLGLW
QKLAHLGTAIENIHVSDRGHFGMTELNAAQFHLDALGQVVELERVGQVLFNELHKSEVKLYCPAKLDQVEAAVEGHTLVL
DTGERIHAKLVVAADGLQSKVRSSFNLPITQVAFEQTAIIANIQTADLPTNRPQKHWAYERFTETGPLALLPMGDSQGQP
RLSLVWALDNARAKQMQTVPKAEFLAALQQAFGYRAGTFIDVSARHAYPLNLSYMPRPIHHRCVFIGNAAQTLHPIAGQG
FNLGLRDVVSLLEVVKSALVQGEDIGSAAVTHAYLHAREDDRNTTIRNIEFLVRGFSNQYWPLALGRNLGLRLLSWFPPL
KTPVAQKAMGWK

Sequences:

>Translated_412_residues
MTVTNHMDTSVQNVDIAIVGGAMAGAILALALARLSHTTAPLSVALVEAHLPDKQHPGFDARSIAIAHGSVFELSRLGLW
QKLAHLGTAIENIHVSDRGHFGMTELNAAQFHLDALGQVVELERVGQVLFNELHKSEVKLYCPAKLDQVEAAVEGHTLVL
DTGERIHAKLVVAADGLQSKVRSSFNLPITQVAFEQTAIIANIQTADLPTNRPQKHWAYERFTETGPLALLPMGDSQGQP
RLSLVWALDNARAKQMQTVPKAEFLAALQQAFGYRAGTFIDVSARHAYPLNLSYMPRPIHHRCVFIGNAAQTLHPIAGQG
FNLGLRDVVSLLEVVKSALVQGEDIGSAAVTHAYLHAREDDRNTTIRNIEFLVRGFSNQYWPLALGRNLGLRLLSWFPPL
KTPVAQKAMGWK
>Mature_411_residues
TVTNHMDTSVQNVDIAIVGGAMAGAILALALARLSHTTAPLSVALVEAHLPDKQHPGFDARSIAIAHGSVFELSRLGLWQ
KLAHLGTAIENIHVSDRGHFGMTELNAAQFHLDALGQVVELERVGQVLFNELHKSEVKLYCPAKLDQVEAAVEGHTLVLD
TGERIHAKLVVAADGLQSKVRSSFNLPITQVAFEQTAIIANIQTADLPTNRPQKHWAYERFTETGPLALLPMGDSQGQPR
LSLVWALDNARAKQMQTVPKAEFLAALQQAFGYRAGTFIDVSARHAYPLNLSYMPRPIHHRCVFIGNAAQTLHPIAGQGF
NLGLRDVVSLLEVVKSALVQGEDIGSAAVTHAYLHAREDDRNTTIRNIEFLVRGFSNQYWPLALGRNLGLRLLSWFPPLK
TPVAQKAMGWK

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=399, Percent_Identity=25.8145363408521, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI32967307, Length=339, Percent_Identity=26.8436578171091, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1789274, Length=400, Percent_Identity=45.5, Blast_Score=330, Evalue=7e-92,
Organism=Escherichia coli, GI1789273, Length=405, Percent_Identity=32.5925925925926, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1786883, Length=381, Percent_Identity=31.758530183727, Blast_Score=154, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI25146631, Length=434, Percent_Identity=26.7281105990783, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321694, Length=454, Percent_Identity=22.4669603524229, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920760, Length=456, Percent_Identity=26.5350877192982, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 45091; Mature: 44960

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTNHMDTSVQNVDIAIVGGAMAGAILALALARLSHTTAPLSVALVEAHLPDKQHPGFD
CCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
ARSIAIAHGSVFELSRLGLWQKLAHLGTAIENIHVSDRGHFGMTELNAAQFHLDALGQVV
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
ELERVGQVLFNELHKSEVKLYCPAKLDQVEAAVEGHTLVLDTGERIHAKLVVAADGLQSK
HHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCEEEEECCCCEEEEEEEEECCHHHH
VRSSFNLPITQVAFEQTAIIANIQTADLPTNRPQKHWAYERFTETGPLALLPMGDSQGQP
HHHCCCCCHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
RLSLVWALDNARAKQMQTVPKAEFLAALQQAFGYRAGTFIDVSARHAYPLNLSYMPRPIH
CEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCCCCCC
HRCVFIGNAAQTLHPIAGQGFNLGLRDVVSLLEVVKSALVQGEDIGSAAVTHAYLHARED
CEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCC
DRNTTIRNIEFLVRGFSNQYWPLALGRNLGLRLLSWFPPLKTPVAQKAMGWK
CCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TVTNHMDTSVQNVDIAIVGGAMAGAILALALARLSHTTAPLSVALVEAHLPDKQHPGFD
CCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
ARSIAIAHGSVFELSRLGLWQKLAHLGTAIENIHVSDRGHFGMTELNAAQFHLDALGQVV
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
ELERVGQVLFNELHKSEVKLYCPAKLDQVEAAVEGHTLVLDTGERIHAKLVVAADGLQSK
HHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCEEEEECCCCEEEEEEEEECCHHHH
VRSSFNLPITQVAFEQTAIIANIQTADLPTNRPQKHWAYERFTETGPLALLPMGDSQGQP
HHHCCCCCHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
RLSLVWALDNARAKQMQTVPKAEFLAALQQAFGYRAGTFIDVSARHAYPLNLSYMPRPIH
CEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCCCCCC
HRCVFIGNAAQTLHPIAGQGFNLGLRDVVSLLEVVKSALVQGEDIGSAAVTHAYLHARED
CEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCC
DRNTTIRNIEFLVRGFSNQYWPLALGRNLGLRLLSWFPPLKTPVAQKAMGWK
CCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]