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The map label for this gene is trmH [H]

Identifier: 119947098

GI number: 119947098

Start: 4327511

End: 4328215

Strand: Reverse

Name: trmH [H]

Synonym: Ping_3496

Alternate gene names: 119947098

Gene position: 4328215-4327511 (Counterclockwise)

Preceding gene: 119947100

Following gene: 119947097

Centisome position: 94.93

GC content: 46.24

Gene sequence:

>705_bases
ATGTCACCCGAACGTCTTGCCCGTATTACGAACATGTTAAATTATCGCCAACCGGATCTGACCGTATTTTTGGAGCAAGT
GCATAAAGCGCATAACTTAGCGGCTATTGTGCGTACTGCGGACGCTGTCGGCGTGAGTGATGTGCATGCCACTTGGGAAA
ATAAAACAACACAGATTTCAGGCAATACGGCAACGGGCAGTCAGAATTGGGTCAATCTGCATTCACATGAAACCCTAGAG
GATGCGGTAAAAGCATTACGTGCTCAGGGCATGCAGATTATTGCGACTAATCTCTCCGATAGTGCGATTGACTTTCGCGA
GGTGGATTATACCAAACCTACAGCCATTTTAATGGGCCAGGAAAAGCATGGCATTAGTCCTGAAGCCCTGGCATTAGCAG
ATCAGGATGTGATTATTCCAATGGTTGGGATGTGCCAGTCATTAAATGTATCGGTTGCCTCTGCGCTTATATTGTATGAA
GCACAACGCCAGCGTGAACTGGCCGGTATGTATAATGGGGAATGCCGTTTACCCGAGGAGGAAGCGCATCGCATTTTATT
TGAAGGCGGGCATCCTATTTTCACTCAATTGTGCAAAGAGAGAAATCTCCCTTATCCTCCTCTGGATGATAAGGGGCAAA
TTGATGCTTCTGATGATTGGTGGCTGGCGGTTCGTTCATCTGTAAAGAGTCTGAAAACTAAATAA

Upstream 100 bases:

>100_bases
GCGTCGTTTATCTAAGATGCGCTGTTTGACTGTCTTTGTGTCCTGCTAATATCATGTATAATAGCCTTCTAAATATAACC
CCTTATTTTTGAGAACGATT

Downstream 100 bases:

>100_bases
GTTCGGGAGAAGTTTCATTGAGTCTATTTCAGCAACGTTTATTAGCTGAAGGTGTACGCACTGTTGAAAGTGATTACCCC
GCTTTATCCGCGCCCATTTT

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSPERLARITNMLNYRQPDLTVFLEQVHKAHNLAAIVRTADAVGVSDVHATWENKTTQISGNTATGSQNWVNLHSHETLE
DAVKALRAQGMQIIATNLSDSAIDFREVDYTKPTAILMGQEKHGISPEALALADQDVIIPMVGMCQSLNVSVASALILYE
AQRQRELAGMYNGECRLPEEEAHRILFEGGHPIFTQLCKERNLPYPPLDDKGQIDASDDWWLAVRSSVKSLKTK

Sequences:

>Translated_234_residues
MSPERLARITNMLNYRQPDLTVFLEQVHKAHNLAAIVRTADAVGVSDVHATWENKTTQISGNTATGSQNWVNLHSHETLE
DAVKALRAQGMQIIATNLSDSAIDFREVDYTKPTAILMGQEKHGISPEALALADQDVIIPMVGMCQSLNVSVASALILYE
AQRQRELAGMYNGECRLPEEEAHRILFEGGHPIFTQLCKERNLPYPPLDDKGQIDASDDWWLAVRSSVKSLKTK
>Mature_233_residues
SPERLARITNMLNYRQPDLTVFLEQVHKAHNLAAIVRTADAVGVSDVHATWENKTTQISGNTATGSQNWVNLHSHETLED
AVKALRAQGMQIIATNLSDSAIDFREVDYTKPTAILMGQEKHGISPEALALADQDVIIPMVGMCQSLNVSVASALILYEA
QRQRELAGMYNGECRLPEEEAHRILFEGGHPIFTQLCKERNLPYPPLDDKGQIDASDDWWLAVRSSVKSLKTK

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=228, Percent_Identity=60.5263157894737, Blast_Score=294, Evalue=3e-81,
Organism=Escherichia coli, GI1790623, Length=174, Percent_Identity=30.4597701149425, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 26004; Mature: 25873

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPERLARITNMLNYRQPDLTVFLEQVHKAHNLAAIVRTADAVGVSDVHATWENKTTQIS
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEC
GNTATGSQNWVNLHSHETLEDAVKALRAQGMQIIATNLSDSAIDFREVDYTKPTAILMGQ
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHCCCCCCEEEEECC
EKHGISPEALALADQDVIIPMVGMCQSLNVSVASALILYEAQRQRELAGMYNGECRLPEE
CCCCCCHHHEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EAHRILFEGGHPIFTQLCKERNLPYPPLDDKGQIDASDDWWLAVRSSVKSLKTK
HHCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPERLARITNMLNYRQPDLTVFLEQVHKAHNLAAIVRTADAVGVSDVHATWENKTTQIS
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEC
GNTATGSQNWVNLHSHETLEDAVKALRAQGMQIIATNLSDSAIDFREVDYTKPTAILMGQ
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHCCCCCCEEEEECC
EKHGISPEALALADQDVIIPMVGMCQSLNVSVASALILYEAQRQRELAGMYNGECRLPEE
CCCCCCHHHEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EAHRILFEGGHPIFTQLCKERNLPYPPLDDKGQIDASDDWWLAVRSSVKSLKTK
HHCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]