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The map label for this gene is cls [H]

Identifier: 119946922

GI number: 119946922

Start: 4091798

End: 4093276

Strand: Reverse

Name: cls [H]

Synonym: Ping_3316

Alternate gene names: 119946922

Gene position: 4093276-4091798 (Counterclockwise)

Preceding gene: 119946923

Following gene: 119946920

Centisome position: 89.77

GC content: 40.64

Gene sequence:

>1479_bases
ATGGACACTTCTTTATTTACAACGAACTGGTCAGATAACTCCTTGTATGTTGGACTGACCGCTTTTATATATATAACGCT
GGTGATACTGACCTCATTAAGTGTTCTTATTAAACGCAAACCCATTGGCGTTTCTCTCGCATGGTTGCTGCTTATTTACG
CTTTACCTATGGTGGGCATGATAAGTTATTATATTTTTGGCCAGCTCAATTTAGGTAAAAAGAGAAAAAAGCGCCGCCTT
GAGATGGCGGGGTTATTTAAAACCTGGCTCGCAGATGAAGTGACAGAACATCGCTTATCTGATCAACAAATTTCCAGCGC
TGTGACGTCGATTAAACATTTTGTTGAATTTGGTAGCCAAATGCCGATGATGGCTGGCAATAAACTCACCTTATTATCCT
CTACTGACGTTATTTTAACGGAGCTGGCGGATTGTATTGAGCAAGCCCGGCACAGCTGTTATTTAATGTTTTATATCTGC
TCGGGGGGAGGGTTGGCAAATAATATTTTAGATGCATTAATGCGCGCCGCGCGCAGGGGGGTGAGCTGTAAGTTATTATT
TGATTCTGTTGGCAGCCATGATTTCTTTAACTCTACAGAATCGCAGAGGCTTAGAGGGGCAGGTGTTGAAGTCTGTGAAG
CCTTACCTGTGGGTACTTTTAGATTACTCTTTCAGCGTCAAGACTTACGTTTACACCGCAAATTAGTTTGTATTGATGAT
CATGTCGCTTATACCGGCAGTATGAACTTAGTTGATCCCGCTTTCTTTAAAAAAGAGAAAAATATCGGTCAATGGGTTGA
TATGATGGTGCGCTGCGAGGGGCCTATCGTGCAGTTGATGAAAAGTTTGTTTGTCTGGGATTGGTATTTGGAAACTAACG
TTAAATTACCTTTTCAAAAACTTTATGCCGATAGTTTTGCCATCGCCGGGGATCAAAATGTGCAGCTTATTCCATCGGGG
CCTGAGTTTGGTGAAGCGAGTATCTATCAAGTTTTATTAATCGCTATTTATGAGGCTAAATATAGTTTGGTGCTCACCAC
TCCCTATTTTGTTCCCGATGAGAGTTTGCATGAAGCATTAAAAGTGGCCGCGCTGCGTGGTGTTGATGTCAAAGTGATTT
TGCCGGCTAAAAATGATTCCTTTATGGTTGATTACGCATCACGGGCCTTTTTTGAGGAGTTGTTGGTTGCGGGGGTGAAA
ATATATAAATTCCATGGCGGTTTACTGCACAGTAAAAGTATTGTTATTGATGAAAAAATTGCTTTATTAGGAACGGTTAA
TCTAGACCGCCGTAGTTTTTGGTTAAATTTTGAAATGACGATGTTGGTTGATAATGATTTTTTTGCCGACGAATTATTGC
AGCTGCAAATGCGTTATTTATTGTTATCAGATCCTTTAATACTGGAAGATTGGCAGAAGCGAAGTTATCACAAGCGCCTA
TTAGAAAGTGTTATGTACCTGTTTAGTCCGCTTTTATAG

Upstream 100 bases:

>100_bases
CACAAATAATCATAGTATTTTAAATCGACTCTCTCGCAATAAAAGTTGTCGTACGCTGTCATTTAAGCTAACGTTATTTT
TTCCATTAAGAACTTGCCAT

Downstream 100 bases:

>100_bases
CTCGAAGGCTTGAAAGCCGTCAGCCGTCAGTCGAAGCTCGAAGACTCGCAGCCGACAACCGACAATCGACAACCGACAAC
CGACAGCTTACCGCCGATAG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MDTSLFTTNWSDNSLYVGLTAFIYITLVILTSLSVLIKRKPIGVSLAWLLLIYALPMVGMISYYIFGQLNLGKKRKKRRL
EMAGLFKTWLADEVTEHRLSDQQISSAVTSIKHFVEFGSQMPMMAGNKLTLLSSTDVILTELADCIEQARHSCYLMFYIC
SGGGLANNILDALMRAARRGVSCKLLFDSVGSHDFFNSTESQRLRGAGVEVCEALPVGTFRLLFQRQDLRLHRKLVCIDD
HVAYTGSMNLVDPAFFKKEKNIGQWVDMMVRCEGPIVQLMKSLFVWDWYLETNVKLPFQKLYADSFAIAGDQNVQLIPSG
PEFGEASIYQVLLIAIYEAKYSLVLTTPYFVPDESLHEALKVAALRGVDVKVILPAKNDSFMVDYASRAFFEELLVAGVK
IYKFHGGLLHSKSIVIDEKIALLGTVNLDRRSFWLNFEMTMLVDNDFFADELLQLQMRYLLLSDPLILEDWQKRSYHKRL
LESVMYLFSPLL

Sequences:

>Translated_492_residues
MDTSLFTTNWSDNSLYVGLTAFIYITLVILTSLSVLIKRKPIGVSLAWLLLIYALPMVGMISYYIFGQLNLGKKRKKRRL
EMAGLFKTWLADEVTEHRLSDQQISSAVTSIKHFVEFGSQMPMMAGNKLTLLSSTDVILTELADCIEQARHSCYLMFYIC
SGGGLANNILDALMRAARRGVSCKLLFDSVGSHDFFNSTESQRLRGAGVEVCEALPVGTFRLLFQRQDLRLHRKLVCIDD
HVAYTGSMNLVDPAFFKKEKNIGQWVDMMVRCEGPIVQLMKSLFVWDWYLETNVKLPFQKLYADSFAIAGDQNVQLIPSG
PEFGEASIYQVLLIAIYEAKYSLVLTTPYFVPDESLHEALKVAALRGVDVKVILPAKNDSFMVDYASRAFFEELLVAGVK
IYKFHGGLLHSKSIVIDEKIALLGTVNLDRRSFWLNFEMTMLVDNDFFADELLQLQMRYLLLSDPLILEDWQKRSYHKRL
LESVMYLFSPLL
>Mature_492_residues
MDTSLFTTNWSDNSLYVGLTAFIYITLVILTSLSVLIKRKPIGVSLAWLLLIYALPMVGMISYYIFGQLNLGKKRKKRRL
EMAGLFKTWLADEVTEHRLSDQQISSAVTSIKHFVEFGSQMPMMAGNKLTLLSSTDVILTELADCIEQARHSCYLMFYIC
SGGGLANNILDALMRAARRGVSCKLLFDSVGSHDFFNSTESQRLRGAGVEVCEALPVGTFRLLFQRQDLRLHRKLVCIDD
HVAYTGSMNLVDPAFFKKEKNIGQWVDMMVRCEGPIVQLMKSLFVWDWYLETNVKLPFQKLYADSFAIAGDQNVQLIPSG
PEFGEASIYQVLLIAIYEAKYSLVLTTPYFVPDESLHEALKVAALRGVDVKVILPAKNDSFMVDYASRAFFEELLVAGVK
IYKFHGGLLHSKSIVIDEKIALLGTVNLDRRSFWLNFEMTMLVDNDFFADELLQLQMRYLLLSDPLILEDWQKRSYHKRL
LESVMYLFSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=471, Percent_Identity=44.1613588110403, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1787007, Length=330, Percent_Identity=26.969696969697, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55964; Mature: 55964

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSLFTTNWSDNSLYVGLTAFIYITLVILTSLSVLIKRKPIGVSLAWLLLIYALPMVGM
CCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ISYYIFGQLNLGKKRKKRRLEMAGLFKTWLADEVTEHRLSDQQISSAVTSIKHFVEFGSQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
MPMMAGNKLTLLSSTDVILTELADCIEQARHSCYLMFYICSGGGLANNILDALMRAARRG
CCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCC
VSCKLLFDSVGSHDFFNSTESQRLRGAGVEVCEALPVGTFRLLFQRQDLRLHRKLVCIDD
CEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEEC
HVAYTGSMNLVDPAFFKKEKNIGQWVDMMVRCEGPIVQLMKSLFVWDWYLETNVKLPFQK
CEEEECCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCCCCCHHH
LYADSFAIAGDQNVQLIPSGPEFGEASIYQVLLIAIYEAKYSLVLTTPYFVPDESLHEAL
HHHCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHH
KVAALRGVDVKVILPAKNDSFMVDYASRAFFEELLVAGVKIYKFHGGLLHSKSIVIDEKI
HHHHHCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEEECCCEEECCEEEEECCE
ALLGTVNLDRRSFWLNFEMTMLVDNDFFADELLQLQMRYLLLSDPLILEDWQKRSYHKRL
EEEEEEECCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LESVMYLFSPLL
HHHHHHHHHCCC
>Mature Secondary Structure
MDTSLFTTNWSDNSLYVGLTAFIYITLVILTSLSVLIKRKPIGVSLAWLLLIYALPMVGM
CCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ISYYIFGQLNLGKKRKKRRLEMAGLFKTWLADEVTEHRLSDQQISSAVTSIKHFVEFGSQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
MPMMAGNKLTLLSSTDVILTELADCIEQARHSCYLMFYICSGGGLANNILDALMRAARRG
CCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCC
VSCKLLFDSVGSHDFFNSTESQRLRGAGVEVCEALPVGTFRLLFQRQDLRLHRKLVCIDD
CEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEEC
HVAYTGSMNLVDPAFFKKEKNIGQWVDMMVRCEGPIVQLMKSLFVWDWYLETNVKLPFQK
CEEEECCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCCCCCHHH
LYADSFAIAGDQNVQLIPSGPEFGEASIYQVLLIAIYEAKYSLVLTTPYFVPDESLHEAL
HHHCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHH
KVAALRGVDVKVILPAKNDSFMVDYASRAFFEELLVAGVKIYKFHGGLLHSKSIVIDEKI
HHHHHCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEEECCCEEECCEEEEECCE
ALLGTVNLDRRSFWLNFEMTMLVDNDFFADELLQLQMRYLLLSDPLILEDWQKRSYHKRL
EEEEEEECCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LESVMYLFSPLL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA