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The map label for this gene is ispH

Identifier: 119946874

GI number: 119946874

Start: 4033300

End: 4034244

Strand: Reverse

Name: ispH

Synonym: Ping_3268

Alternate gene names: 119946874

Gene position: 4034244-4033300 (Counterclockwise)

Preceding gene: 119946875

Following gene: 119946873

Centisome position: 88.48

GC content: 43.17

Gene sequence:

>945_bases
ATGGAAATCATTTTAGCGAATCCGCGTGGTTTTTGTGCGGGTGTCCATCGTGCTATTTCAATTGTTGATCGTGCCTTAGA
TATTTTTACACCCCCCATTTATGTGCGTCATGAAGTTGTTCATAACCAGTATGTGGTTGATGACTTAAGAGCCCGTGGCG
CCGTATTTGTCGAAGAGTTAGATGAAGTACCTGAGAACAGTATTGTTATTTTTAGTGCACATGGTGTCGCACAATCGGTG
CGCGCAGAAGCCAAACGGCGCGATCTTAAAATCTTTGATGCAACTTGTCCGTTAGTAACTAAAGTGCATATGGAAGTGAC
GCGTGCCAGTCGTCGCGGGCAGGAATGTATTTTAATAGGTCATCAAGGCCATCCTGAGGTCGTTGGTACTATGGGGCAAT
ATGATAACCCACAGGGTGGGATTTATTTGATTGAATCGCCTGAAGATATCAAAAAATTAGAGGTTAAAAATCCCAACAGT
CTTTGTTTTAGTTCTCAAACGACTCTGTCGGTTGATGATACCGCAAATATTATCAAGGCATTGCGCGAGAAATTTCCTAA
GATTGATGGGCCACGTAAAGATGATATCTGTTATGCAACACAAAATCGTCAGGATGCCGTTAAAGCGCTTGCTGAAAGAA
GTGATCTTGTTTTAGTGGTTGGCTCGACCAATTCTTCGAATTCCAATCGTTTACAGGAAAAAGCAAGTTGTGTCGGTGTG
AATGCCTATCTTATTGACAGTGAAAAAGATATTAAAACTGAGTGGCTTGAAAATGTAAAAGTGGTTGGCGTTACTGCGGG
GGCCTCTGCCCCGGAAGTGCTGGTGAAAGAGGTGATTGCCAGGCTCAAAAAACTAGGTGGTACTGTGGTGACTGAAAATT
TGGGTGTTGAAGAAAATATTATCTTTGAAGTGCCCGCCGAGCTGCGCATTAAGCAGATCGATTGA

Upstream 100 bases:

>100_bases
CTTGCCGGTCAAACGGTTACCTTTGAAGTGGATGTATTAGAAGTTTTAAACTAAATAGCTTTGAGCTTTGAGCTTCGAGC
TTCTTAAATAAAGGTTATTT

Downstream 100 bases:

>100_bases
TTTTTATAAAATGACTTTTCTTTTTTAGAGTGGAATACATTTGAAAATACAACTGGCGTAGCATTTTTTGCATTTTCTAT
CTGTATATTTACCGTCATTA

Product: hydroxymethylbutenyl pyrophosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MEIILANPRGFCAGVHRAISIVDRALDIFTPPIYVRHEVVHNQYVVDDLRARGAVFVEELDEVPENSIVIFSAHGVAQSV
RAEAKRRDLKIFDATCPLVTKVHMEVTRASRRGQECILIGHQGHPEVVGTMGQYDNPQGGIYLIESPEDIKKLEVKNPNS
LCFSSQTTLSVDDTANIIKALREKFPKIDGPRKDDICYATQNRQDAVKALAERSDLVLVVGSTNSSNSNRLQEKASCVGV
NAYLIDSEKDIKTEWLENVKVVGVTAGASAPEVLVKEVIARLKKLGGTVVTENLGVEENIIFEVPAELRIKQID

Sequences:

>Translated_314_residues
MEIILANPRGFCAGVHRAISIVDRALDIFTPPIYVRHEVVHNQYVVDDLRARGAVFVEELDEVPENSIVIFSAHGVAQSV
RAEAKRRDLKIFDATCPLVTKVHMEVTRASRRGQECILIGHQGHPEVVGTMGQYDNPQGGIYLIESPEDIKKLEVKNPNS
LCFSSQTTLSVDDTANIIKALREKFPKIDGPRKDDICYATQNRQDAVKALAERSDLVLVVGSTNSSNSNRLQEKASCVGV
NAYLIDSEKDIKTEWLENVKVVGVTAGASAPEVLVKEVIARLKKLGGTVVTENLGVEENIIFEVPAELRIKQID
>Mature_314_residues
MEIILANPRGFCAGVHRAISIVDRALDIFTPPIYVRHEVVHNQYVVDDLRARGAVFVEELDEVPENSIVIFSAHGVAQSV
RAEAKRRDLKIFDATCPLVTKVHMEVTRASRRGQECILIGHQGHPEVVGTMGQYDNPQGGIYLIESPEDIKKLEVKNPNS
LCFSSQTTLSVDDTANIIKALREKFPKIDGPRKDDICYATQNRQDAVKALAERSDLVLVVGSTNSSNSNRLQEKASCVGV
NAYLIDSEKDIKTEWLENVKVVGVTAGASAPEVLVKEVIARLKKLGGTVVTENLGVEENIIFEVPAELRIKQID

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=316, Percent_Identity=68.6708860759494, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_PSYIN (A1SZP0)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944554.1
- STRING:   A1SZP0
- GeneID:   4626301
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3268
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   A1SZP0
- BioCyc:   PING357804:PING_3268-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34607; Mature: 34607

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIILANPRGFCAGVHRAISIVDRALDIFTPPIYVRHEVVHNQYVVDDLRARGAVFVEEL
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHCCCHHHHHHH
DEVPENSIVIFSAHGVAQSVRAEAKRRDLKIFDATCPLVTKVHMEVTRASRRGQECILIG
HHCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEC
HQGHPEVVGTMGQYDNPQGGIYLIESPEDIKKLEVKNPNSLCFSSQTTLSVDDTANIIKA
CCCCCHHEECCCCCCCCCCCEEEEECCCCHHHEECCCCCCEEECCCCEEECCHHHHHHHH
LREKFPKIDGPRKDDICYATQNRQDAVKALAERSDLVLVVGSTNSSNSNRLQEKASCVGV
HHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHEEEE
NAYLIDSEKDIKTEWLENVKVVGVTAGASAPEVLVKEVIARLKKLGGTVVTENLGVEENI
EEEEECCCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
IFEVPAELRIKQID
EEECCHHCEEEECC
>Mature Secondary Structure
MEIILANPRGFCAGVHRAISIVDRALDIFTPPIYVRHEVVHNQYVVDDLRARGAVFVEEL
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHCCCHHHHHHH
DEVPENSIVIFSAHGVAQSVRAEAKRRDLKIFDATCPLVTKVHMEVTRASRRGQECILIG
HHCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEC
HQGHPEVVGTMGQYDNPQGGIYLIESPEDIKKLEVKNPNSLCFSSQTTLSVDDTANIIKA
CCCCCHHEECCCCCCCCCCCEEEEECCCCHHHEECCCCCCEEECCCCEEECCHHHHHHHH
LREKFPKIDGPRKDDICYATQNRQDAVKALAERSDLVLVVGSTNSSNSNRLQEKASCVGV
HHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHEEEE
NAYLIDSEKDIKTEWLENVKVVGVTAGASAPEVLVKEVIARLKKLGGTVVTENLGVEENI
EEEEECCCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
IFEVPAELRIKQID
EEECCHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA