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The map label for this gene is bioH [H]

Identifier: 119946806

GI number: 119946806

Start: 3953975

End: 3954754

Strand: Reverse

Name: bioH [H]

Synonym: Ping_3200

Alternate gene names: 119946806

Gene position: 3954754-3953975 (Counterclockwise)

Preceding gene: 119946810

Following gene: 119946803

Centisome position: 86.73

GC content: 39.23

Gene sequence:

>780_bases
GTGATTATAGAACAAAGAGTATATTGCCGTGTGCTGGGTGAAGGGCCCAATTTGTTGTTATTACATGGCTGGGGGGTGAA
CTCTGCCGTTTGGGATCCGGTTCTTTATGGATTATCTGCTCATTTTCGAGTGCATTTAATTGATCTCCCTGGATTTGGCC
GCAGTGAAGGATTAGCTGAATATTCTCTTGATACAATAGTCGAGCGTATTATGGAAAGTGTGCCTGAGCAGTCAATCTGG
TGTGGTTGGTCTCTGGGTGGTTTAATTGCAAGTCATGCCAGTTACTTATACCCCAATAAAATTGCTAAATTAATTCAAGT
TTGTACTTCGCCCAAATTTGTTGGCGACCTACTCTGGCCGGGTGTTGAAACTATTGTGTTTGAAAACTTCAAATTGGGGC
TGCTTAAAAACTCAGATAAAACATTGGCTCGCTTTATCAGCATTCAAGCAATGGGCTGTGAAAGTACACGTAAAGACACG
CTCATATTAAAAAAACTTTTGAAGGATACAAAGCAAGCCTCTTTGATGGCTTTAAGTGAAGGCTTAAATCTTCTCAGTGA
GAGTGATTTACGAAGGGAATTTGCTCAGATTAGTCTCCCTTGTTTATCCATTTTTGGTCAGTTTGACAGTTTAGTCCCTA
TTGAAACAAGTGTTGCTATGCAGCAATTACTGCCAGACTCCCTGAAAAAAGTATTTATGCACTCATCGCATGCGCCATTT
ATTAGCGAACCTGAGGAATTTATTGATAATATTATTAGTTTCTCATTAATTAAAAATTAA

Upstream 100 bases:

>100_bases
CGGGCAGGTAAGCATTGTGCGATTAATTTTTTGCATTTTTGTTTAATAAAGGCAGTAACTGTTTGCACCAGCGTTTATCC
AATTAAAAGAGAGAGAGAAT

Downstream 100 bases:

>100_bases
TTTTTAACTTTATGGATCTTTGATTAATTTATGAAAAGGGGGAGTCAGTTTTTGATTAACAGAAGGTAAAAAGAGGTTGT
TATCTTTAAAGGATGAAAAT

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MIIEQRVYCRVLGEGPNLLLLHGWGVNSAVWDPVLYGLSAHFRVHLIDLPGFGRSEGLAEYSLDTIVERIMESVPEQSIW
CGWSLGGLIASHASYLYPNKIAKLIQVCTSPKFVGDLLWPGVETIVFENFKLGLLKNSDKTLARFISIQAMGCESTRKDT
LILKKLLKDTKQASLMALSEGLNLLSESDLRREFAQISLPCLSIFGQFDSLVPIETSVAMQQLLPDSLKKVFMHSSHAPF
ISEPEEFIDNIISFSLIKN

Sequences:

>Translated_259_residues
MIIEQRVYCRVLGEGPNLLLLHGWGVNSAVWDPVLYGLSAHFRVHLIDLPGFGRSEGLAEYSLDTIVERIMESVPEQSIW
CGWSLGGLIASHASYLYPNKIAKLIQVCTSPKFVGDLLWPGVETIVFENFKLGLLKNSDKTLARFISIQAMGCESTRKDT
LILKKLLKDTKQASLMALSEGLNLLSESDLRREFAQISLPCLSIFGQFDSLVPIETSVAMQQLLPDSLKKVFMHSSHAPF
ISEPEEFIDNIISFSLIKN
>Mature_259_residues
MIIEQRVYCRVLGEGPNLLLLHGWGVNSAVWDPVLYGLSAHFRVHLIDLPGFGRSEGLAEYSLDTIVERIMESVPEQSIW
CGWSLGGLIASHASYLYPNKIAKLIQVCTSPKFVGDLLWPGVETIVFENFKLGLLKNSDKTLARFISIQAMGCESTRKDT
LILKKLLKDTKQASLMALSEGLNLLSESDLRREFAQISLPCLSIFGQFDSLVPIETSVAMQQLLPDSLKKVFMHSSHAPF
ISEPEEFIDNIISFSLIKN

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=249, Percent_Identity=37.7510040160643, Blast_Score=198, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28914; Mature: 28914

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEQRVYCRVLGEGPNLLLLHGWGVNSAVWDPVLYGLSAHFRVHLIDLPGFGRSEGLAE
CCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHH
YSLDTIVERIMESVPEQSIWCGWSLGGLIASHASYLYPNKIAKLIQVCTSPKFVGDLLWP
HHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCC
GVETIVFENFKLGLLKNSDKTLARFISIQAMGCESTRKDTLILKKLLKDTKQASLMALSE
CHHHHHHCCCEEEEEECCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLNLLSESDLRREFAQISLPCLSIFGQFDSLVPIETSVAMQQLLPDSLKKVFMHSSHAPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
ISEPEEFIDNIISFSLIKN
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIIEQRVYCRVLGEGPNLLLLHGWGVNSAVWDPVLYGLSAHFRVHLIDLPGFGRSEGLAE
CCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHH
YSLDTIVERIMESVPEQSIWCGWSLGGLIASHASYLYPNKIAKLIQVCTSPKFVGDLLWP
HHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCC
GVETIVFENFKLGLLKNSDKTLARFISIQAMGCESTRKDTLILKKLLKDTKQASLMALSE
CHHHHHHCCCEEEEEECCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLNLLSESDLRREFAQISLPCLSIFGQFDSLVPIETSVAMQQLLPDSLKKVFMHSSHAPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
ISEPEEFIDNIISFSLIKN
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA