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The map label for this gene is miaE [H]

Identifier: 119946700

GI number: 119946700

Start: 3782988

End: 3783749

Strand: Reverse

Name: miaE [H]

Synonym: Ping_3082

Alternate gene names: 119946700

Gene position: 3783749-3782988 (Counterclockwise)

Preceding gene: 119946703

Following gene: 119946699

Centisome position: 82.98

GC content: 35.7

Gene sequence:

>762_bases
ATGTATGAAGAATTATTAGCGCCTATTAACTCCTTTTTAAAATGTAGTACGCCTGATTCTTGGGTTGATCAAGCTAAAAA
AGCAGAAAATTTACCCATTCTTCTTCGGGATCACTTGATATGTGAATTAAAAGCTTCTCAAAGTGCCCTGTTTTTACTTA
AGCGTTATGCCCTAAGAGAAGAGGGGATAGAGCAACTAGGGGAACTTGTAAAACCTTATGAACAATTTGCTTATAAGCGT
ATCGGTAATCTGGAAACCCTTAAAGGTAAGAATGCTATATCAAAGTCGATTAAGGCGCGTTCAGACTGTCCTTACGGTCA
GGATATGGTCGATAAAATGGTTTTATTGATAAGAGAAGAATTACATCACTTTTATCAGGTATTAGAAATTATTGAAGACA
ATAATATTGCTTATGAACCAATACCTGGATCACGTTATGCAAAAGGGTTGTTAAAAAATGTTCGCACTTATGAACCGGAA
GCGCTTATTGATAAATTGATAATCGGTGCTTTTATAGAAGCAAGATCCTGTGAAAGGTTTGCTAAAATCGCCCCACATTT
AGATCCTAAAATAGGAAAGTTTTATTTATCTCTACTCCGTTCAGAAGCACGACATTATCAAGATTATTTAGCATTAGCAG
AGCATATTTCAGGGCAAGACATAACCGAGAGAGTTACTTTTTTTGCTGTTGTTGAAGCTGAGCTCATTCAATCACCGGAT
TCTGACTTTAAATTCCATTCAGGAAAGCCAAACTTTTTTTAA

Upstream 100 bases:

>100_bases
CTAATCTGAATGGCCTTTATATCGATAGCATCAATCAATACGTCAGACTAGATTTCCGTGTTAAAATATTACAAATCATC
AATAAACTGAATTAAAAATA

Downstream 100 bases:

>100_bases
GGCTCTATTCCATTTTATTGTTGCCCCCATGTATCTCTTTTAGCTGGGTTTTGCTTGAATCTTATTCGTAGGTCGAAGAT
CACTATACCAATGTTATTTA

Product: tRNA-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MYEELLAPINSFLKCSTPDSWVDQAKKAENLPILLRDHLICELKASQSALFLLKRYALREEGIEQLGELVKPYEQFAYKR
IGNLETLKGKNAISKSIKARSDCPYGQDMVDKMVLLIREELHHFYQVLEIIEDNNIAYEPIPGSRYAKGLLKNVRTYEPE
ALIDKLIIGAFIEARSCERFAKIAPHLDPKIGKFYLSLLRSEARHYQDYLALAEHISGQDITERVTFFAVVEAELIQSPD
SDFKFHSGKPNFF

Sequences:

>Translated_253_residues
MYEELLAPINSFLKCSTPDSWVDQAKKAENLPILLRDHLICELKASQSALFLLKRYALREEGIEQLGELVKPYEQFAYKR
IGNLETLKGKNAISKSIKARSDCPYGQDMVDKMVLLIREELHHFYQVLEIIEDNNIAYEPIPGSRYAKGLLKNVRTYEPE
ALIDKLIIGAFIEARSCERFAKIAPHLDPKIGKFYLSLLRSEARHYQDYLALAEHISGQDITERVTFFAVVEAELIQSPD
SDFKFHSGKPNFF
>Mature_253_residues
MYEELLAPINSFLKCSTPDSWVDQAKKAENLPILLRDHLICELKASQSALFLLKRYALREEGIEQLGELVKPYEQFAYKR
IGNLETLKGKNAISKSIKARSDCPYGQDMVDKMVLLIREELHHFYQVLEIIEDNNIAYEPIPGSRYAKGLLKNVRTYEPE
ALIDKLIIGAFIEARSCERFAKIAPHLDPKIGKFYLSLLRSEARHYQDYLALAEHISGQDITERVTFFAVVEAELIQSPD
SDFKFHSGKPNFF

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 29058; Mature: 29058

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEELLAPINSFLKCSTPDSWVDQAKKAENLPILLRDHLICELKASQSALFLLKRYALRE
CHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
EGIEQLGELVKPYEQFAYKRIGNLETLKGKNAISKSIKARSDCPYGQDMVDKMVLLIREE
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LHHFYQVLEIIEDNNIAYEPIPGSRYAKGLLKNVRTYEPEALIDKLIIGAFIEARSCERF
HHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AKIAPHLDPKIGKFYLSLLRSEARHYQDYLALAEHISGQDITERVTFFAVVEAELIQSPD
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
SDFKFHSGKPNFF
CCCCCCCCCCCCC
>Mature Secondary Structure
MYEELLAPINSFLKCSTPDSWVDQAKKAENLPILLRDHLICELKASQSALFLLKRYALRE
CHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
EGIEQLGELVKPYEQFAYKRIGNLETLKGKNAISKSIKARSDCPYGQDMVDKMVLLIREE
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LHHFYQVLEIIEDNNIAYEPIPGSRYAKGLLKNVRTYEPEALIDKLIIGAFIEARSCERF
HHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AKIAPHLDPKIGKFYLSLLRSEARHYQDYLALAEHISGQDITERVTFFAVVEAELIQSPD
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
SDFKFHSGKPNFF
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]