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The map label for this gene is mii [H]

Identifier: 119945561

GI number: 119945561

Start: 2268098

End: 2269291

Strand: Reverse

Name: mii [H]

Synonym: Ping_1866

Alternate gene names: 119945561

Gene position: 2269291-2268098 (Counterclockwise)

Preceding gene: 119945562

Following gene: 119945560

Centisome position: 49.77

GC content: 47.4

Gene sequence:

>1194_bases
ATGACGGATAAAACAGCTGACTATCTGCCGCAACTCAGGATCCCCGCTACTTATATGCGCGGGGGAACCAGTAAAGGCGT
TTTTTTTAATTGTCAGGATTTACCCCAGGCGGCGCAAGTTGCCGGGCAAGCACGTGATGCTTTACTGCTGCGTGTTATCG
GCAGTCCAGATCCCTACGGTAAACAAACCGATGGGATGGGCGGCGCGACCTCCAGTACCAGTAAAACGGTTATTCTTTCC
AGCAGTGAAAAAAATGATCACGATGTGAATTATCTCTTTGGTCAGGTTGCCATTGATAAAGCCTTTATAGACTGGAGTGG
TAATTGTGGCAATTTATCAGCAGCTGTTGGCGCATTTGCTATCAACGCAGGTTTAGTCGATAGCAGCCGCATTCCTGCTA
ACGGGATTGCCGTGGTACGTATCTGGCAGGAAAATATCAGTAAAACCATTATCGCCCATATTCCTATTTGTGATGGCGAG
GTACAGGAAATGGGTGACTTTGAGCTTGATGGTGTCACTTTCCCTGCCGCTGAAATTAAAGTCGAATTTATCGACCCTTC
TGATGCTGAAAATAGAATGTTTCCCACGGGCAATATTGTTGATCAGTTGGACGTTCCAGGTATTGGTAATTTTAAAGTAA
CAATGATTAATGCAGGTATTGCCACTGTTTTTTTGAATGCAAAGGAACTGGGTTATCAGGGTACTGAGCTGCAGGAAGCG
ATTAATTCGAATCCTAAGGCGCTTGAGATGTTTGAAACCATACGTGCTTTTGCGGCCCTAAAAATGGGACTCATTGAGCA
TATTGATCAAGCAGTCACGCGGCAGCATACGCCTAAAATTGCTTTTGTTAGCAAGGCGCAGGATTATATTGCCTCTAGCG
GTAAATCTATTAATAAAGAGCGAATAGATTTGCATGTGCGCGCTTTGTCTATGGGGAAACTTCACCATGCAATGATGGGT
ACGGCGGCAGTCGCTATCGGTACAGCCGCGGCGATACCAGGCACCTTAGTCAATTTGGCAGCAGGGGGTGGCGAGCGCCA
GTCGGTTACCTTCGGTCATCCTTCGGGTGTCTTAAAGGTCGGTGCACAAGCCAGCCTGCAAGATGGCCAATGGTCGGTCG
ATAAAGCTGTTATGAGCCGCAGCGCACGTATCTTAATGGAAGGCTGGGTACGTATTCCGGCAACCACTCTATAG

Upstream 100 bases:

>100_bases
GATTAGATACCGAAGAAGAGGTATCAATTTACGAAGCGGGCGGTATTCTGCAGCGCTTTGCGCAAGATTTTATCGAGTCA
TCAAAGGAAGAGGTTTAAAC

Downstream 100 bases:

>100_bases
ACTAATTTTACAGGCATTAATAAAAGGAAATATGATGATTTCTGCATATCAAAATGAATTTAATCGCGCTAAGAATGATC
CTCTGACTTTTTGGCAGGAA

Product: putative AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTDKTADYLPQLRIPATYMRGGTSKGVFFNCQDLPQAAQVAGQARDALLLRVIGSPDPYGKQTDGMGGATSSTSKTVILS
SSEKNDHDVNYLFGQVAIDKAFIDWSGNCGNLSAAVGAFAINAGLVDSSRIPANGIAVVRIWQENISKTIIAHIPICDGE
VQEMGDFELDGVTFPAAEIKVEFIDPSDAENRMFPTGNIVDQLDVPGIGNFKVTMINAGIATVFLNAKELGYQGTELQEA
INSNPKALEMFETIRAFAALKMGLIEHIDQAVTRQHTPKIAFVSKAQDYIASSGKSINKERIDLHVRALSMGKLHHAMMG
TAAVAIGTAAAIPGTLVNLAAGGGERQSVTFGHPSGVLKVGAQASLQDGQWSVDKAVMSRSARILMEGWVRIPATTL

Sequences:

>Translated_397_residues
MTDKTADYLPQLRIPATYMRGGTSKGVFFNCQDLPQAAQVAGQARDALLLRVIGSPDPYGKQTDGMGGATSSTSKTVILS
SSEKNDHDVNYLFGQVAIDKAFIDWSGNCGNLSAAVGAFAINAGLVDSSRIPANGIAVVRIWQENISKTIIAHIPICDGE
VQEMGDFELDGVTFPAAEIKVEFIDPSDAENRMFPTGNIVDQLDVPGIGNFKVTMINAGIATVFLNAKELGYQGTELQEA
INSNPKALEMFETIRAFAALKMGLIEHIDQAVTRQHTPKIAFVSKAQDYIASSGKSINKERIDLHVRALSMGKLHHAMMG
TAAVAIGTAAAIPGTLVNLAAGGGERQSVTFGHPSGVLKVGAQASLQDGQWSVDKAVMSRSARILMEGWVRIPATTL
>Mature_396_residues
TDKTADYLPQLRIPATYMRGGTSKGVFFNCQDLPQAAQVAGQARDALLLRVIGSPDPYGKQTDGMGGATSSTSKTVILSS
SEKNDHDVNYLFGQVAIDKAFIDWSGNCGNLSAAVGAFAINAGLVDSSRIPANGIAVVRIWQENISKTIIAHIPICDGEV
QEMGDFELDGVTFPAAEIKVEFIDPSDAENRMFPTGNIVDQLDVPGIGNFKVTMINAGIATVFLNAKELGYQGTELQEAI
NSNPKALEMFETIRAFAALKMGLIEHIDQAVTRQHTPKIAFVSKAQDYIASSGKSINKERIDLHVRALSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGGERQSVTFGHPSGVLKVGAQASLQDGQWSVDKAVMSRSARILMEGWVRIPATTL

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=382, Percent_Identity=31.413612565445, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 42232; Mature: 42100

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKTADYLPQLRIPATYMRGGTSKGVFFNCQDLPQAAQVAGQARDALLLRVIGSPDPYG
CCCCCHHHCCCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCEEEEEEECCCCCCC
KQTDGMGGATSSTSKTVILSSSEKNDHDVNYLFGQVAIDKAFIDWSGNCGNLSAAVGAFA
CCCCCCCCCCCCCCEEEEEECCCCCCCCHHEEEEEEEEEEEEEECCCCCCCHHHHHHHHE
INAGLVDSSRIPANGIAVVRIWQENISKTIIAHIPICDGEVQEMGDFELDGVTFPAAEIK
EECCCCCCCCCCCCCEEEEEEEHHCCCCEEEEEECCCCCHHHHCCCEEECCEEECCEEEE
VEFIDPSDAENRMFPTGNIVDQLDVPGIGNFKVTMINAGIATVFLNAKELGYQGTELQEA
EEEECCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEEECCEEEEEEEHHHHCCCCHHHHHH
INSNPKALEMFETIRAFAALKMGLIEHIDQAVTRQHTPKIAFVSKAQDYIASSGKSINKE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCH
RIDLHVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERQSVTFGHPSGVLKV
HEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEECCCCCEEEE
GAQASLQDGQWSVDKAVMSRSARILMEGWVRIPATTL
CCCCCCCCCCCHHHHHHHHHHHHEEECCCEECCCCCC
>Mature Secondary Structure 
TDKTADYLPQLRIPATYMRGGTSKGVFFNCQDLPQAAQVAGQARDALLLRVIGSPDPYG
CCCCHHHCCCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCEEEEEEECCCCCCC
KQTDGMGGATSSTSKTVILSSSEKNDHDVNYLFGQVAIDKAFIDWSGNCGNLSAAVGAFA
CCCCCCCCCCCCCCEEEEEECCCCCCCCHHEEEEEEEEEEEEEECCCCCCCHHHHHHHHE
INAGLVDSSRIPANGIAVVRIWQENISKTIIAHIPICDGEVQEMGDFELDGVTFPAAEIK
EECCCCCCCCCCCCCEEEEEEEHHCCCCEEEEEECCCCCHHHHCCCEEECCEEECCEEEE
VEFIDPSDAENRMFPTGNIVDQLDVPGIGNFKVTMINAGIATVFLNAKELGYQGTELQEA
EEEECCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEEECCEEEEEEEHHHHCCCCHHHHHH
INSNPKALEMFETIRAFAALKMGLIEHIDQAVTRQHTPKIAFVSKAQDYIASSGKSINKE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCH
RIDLHVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERQSVTFGHPSGVLKV
HEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEECCCCCEEEE
GAQASLQDGQWSVDKAVMSRSARILMEGWVRIPATTL
CCCCCCCCCCCHHHHHHHHHHHHEEECCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA