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The map label for this gene is murE [H]

Identifier: 119944898

GI number: 119944898

Start: 1429469

End: 1430998

Strand: Direct

Name: murE [H]

Synonym: Ping_1142

Alternate gene names: 119944898

Gene position: 1429469-1430998 (Clockwise)

Preceding gene: 119944897

Following gene: 119944899

Centisome position: 31.35

GC content: 41.31

Gene sequence:

>1530_bases
ATGCATAACGACTCGCAAATAATGTTATTAGACGATTTGATTAAACCTTGGTTAAGTGACTATGATCCTTCGATTAGGGT
CTCCGGTCTAACCTTAGACAGTCGTCAAATTTGCCGTGGTTACCTGTTTATCGCACTTCAGGGAGCACAACATGACGGGC
GTTTATTTATTGAAAAAAGCCTCAAAATGGGCGCTTGTGCTGTTTTATCGGAAACTAAAAACCGATCCGAGCATGGTCAG
GTAACTATAAAATCAGGTGTGCCAGTGTTCGCAGTTTACAAACCTGCGGCTATTTTAAGTGCACTAGCCTATCGTTTCTT
TTTTAGGAAAATCAAGTTACATAAAGTGATTGCTGTGACGGGGACCAATGGAAAAACCACGATAGCAAGTTTGTTAGCTG
ACAGTTTTATGCTCTTAGGTAAACAGAGCGCGCAAATGGGCACCATTGGAAACGGTTTATTCGGGCAATTAAAAAGCAGC
TTAAATACAACCTTAGACGCTATATCGGTATTTTCTGAGTTGCATTGTTATCAGCAGCAGAGGGCTGAATATACGATAAT
GGAAGTCTCCTCTCATGGACTGGACCAGGGGCGTGTTTCCGCCGTACCTTTTTTTAGCGCAATATTTACTAATTTAACCC
GCGATCACCTTGATTATCATGGCTCGATGGAGAATTATCATTTAGCTAAAAAAAGCCTATTTGAAAAGTATCCTCTCAAG
CAACGTATCATCAATGCAGATGACCCAATCGGCGCACAGTGGTTAACTGATTTTCCGGATGCTATCAGTTATGGTTTTAA
TGCCAAAGCCTTAACTGATAAACAATCTTATCTACGGGTAACAGATGTTGATTATCATGCTCAAGGTGTCACTTTTGCAT
TTCACTCAAGTTGGGGGAAGGGAGTCGTATCAGCCCCCTTTTATGGTGATTTCAATGTGTCAAATTTGGCTGCTGTATTA
ACGCAATTATTGGTAGAAAATGTCAGCATAGAACATGTACAAACAATCTTTCCTAAATTAAGTACCGTGCCTGGGCGGAT
GGAACAGTATTACTTTGCGGAAAAAAACATCACCTTTGTGGTTGATTACGCACATACACCCGATGCATTAGAGAAGGTAT
TACTGGCACTGCAGAAACATAAATCTTCAGGTCGTCTGATTTGTGTTTTCGGCTGTGGTGGGGACCGCGATACGGGTAAA
CGTCCACAAATGGCTAACATTGCGGAATCTTATGCTCAAAAAGTGGTGTTGGTTGATGATAATCCACGCACAGAAAATGC
TCAGAGTATCATTGAAGATATATTACAAGGTTTTGCCAGACCCGATAGAGTTGAGGTGATCCATGATCGCCGCCAAGCAG
TGAATTTTTTATTAAATTCAGCACAATCCGATGATATTGTGCTGCTGGCTGGGAAGGGACATGAAGATTACCAAATCTTT
GGCACACAACGCGTGCATTATAGCGATCGAGAATGTTTAGCTTCATTACAAAAATCAGCCGATCTGGTCGATTATTCTGC
ACATAAATAA

Upstream 100 bases:

>100_bases
CGCCGGTTTTTTCAGCTGTGATGAAAAGTGCACTTTTATTAAGTCACGTTCGTCCTGATGATGAGAATGATAGTTATACT
TATTTAGAGTCAAATTAATC

Downstream 100 bases:

>100_bases
GCACAACCTATCTGTAAGAAATAATGGTAAAGTATGATAAAAAGTGACTGTCACGCCAGAGCGAGCAGGCATAATAATGA
CCTTTACCACAATAAAAAAA

Product: UDP-N-acetylmuramyl-tripeptide synthetases

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MHNDSQIMLLDDLIKPWLSDYDPSIRVSGLTLDSRQICRGYLFIALQGAQHDGRLFIEKSLKMGACAVLSETKNRSEHGQ
VTIKSGVPVFAVYKPAAILSALAYRFFFRKIKLHKVIAVTGTNGKTTIASLLADSFMLLGKQSAQMGTIGNGLFGQLKSS
LNTTLDAISVFSELHCYQQQRAEYTIMEVSSHGLDQGRVSAVPFFSAIFTNLTRDHLDYHGSMENYHLAKKSLFEKYPLK
QRIINADDPIGAQWLTDFPDAISYGFNAKALTDKQSYLRVTDVDYHAQGVTFAFHSSWGKGVVSAPFYGDFNVSNLAAVL
TQLLVENVSIEHVQTIFPKLSTVPGRMEQYYFAEKNITFVVDYAHTPDALEKVLLALQKHKSSGRLICVFGCGGDRDTGK
RPQMANIAESYAQKVVLVDDNPRTENAQSIIEDILQGFARPDRVEVIHDRRQAVNFLLNSAQSDDIVLLAGKGHEDYQIF
GTQRVHYSDRECLASLQKSADLVDYSAHK

Sequences:

>Translated_509_residues
MHNDSQIMLLDDLIKPWLSDYDPSIRVSGLTLDSRQICRGYLFIALQGAQHDGRLFIEKSLKMGACAVLSETKNRSEHGQ
VTIKSGVPVFAVYKPAAILSALAYRFFFRKIKLHKVIAVTGTNGKTTIASLLADSFMLLGKQSAQMGTIGNGLFGQLKSS
LNTTLDAISVFSELHCYQQQRAEYTIMEVSSHGLDQGRVSAVPFFSAIFTNLTRDHLDYHGSMENYHLAKKSLFEKYPLK
QRIINADDPIGAQWLTDFPDAISYGFNAKALTDKQSYLRVTDVDYHAQGVTFAFHSSWGKGVVSAPFYGDFNVSNLAAVL
TQLLVENVSIEHVQTIFPKLSTVPGRMEQYYFAEKNITFVVDYAHTPDALEKVLLALQKHKSSGRLICVFGCGGDRDTGK
RPQMANIAESYAQKVVLVDDNPRTENAQSIIEDILQGFARPDRVEVIHDRRQAVNFLLNSAQSDDIVLLAGKGHEDYQIF
GTQRVHYSDRECLASLQKSADLVDYSAHK
>Mature_509_residues
MHNDSQIMLLDDLIKPWLSDYDPSIRVSGLTLDSRQICRGYLFIALQGAQHDGRLFIEKSLKMGACAVLSETKNRSEHGQ
VTIKSGVPVFAVYKPAAILSALAYRFFFRKIKLHKVIAVTGTNGKTTIASLLADSFMLLGKQSAQMGTIGNGLFGQLKSS
LNTTLDAISVFSELHCYQQQRAEYTIMEVSSHGLDQGRVSAVPFFSAIFTNLTRDHLDYHGSMENYHLAKKSLFEKYPLK
QRIINADDPIGAQWLTDFPDAISYGFNAKALTDKQSYLRVTDVDYHAQGVTFAFHSSWGKGVVSAPFYGDFNVSNLAAVL
TQLLVENVSIEHVQTIFPKLSTVPGRMEQYYFAEKNITFVVDYAHTPDALEKVLLALQKHKSSGRLICVFGCGGDRDTGK
RPQMANIAESYAQKVVLVDDNPRTENAQSIIEDILQGFARPDRVEVIHDRRQAVNFLLNSAQSDDIVLLAGKGHEDYQIF
GTQRVHYSDRECLASLQKSADLVDYSAHK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=488, Percent_Identity=46.3114754098361, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1786274, Length=362, Percent_Identity=22.0994475138122, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 56724; Mature: 56724

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNDSQIMLLDDLIKPWLSDYDPSIRVSGLTLDSRQICRGYLFIALQGAQHDGRLFIEKS
CCCCCEEEEHHHHHHHHHHCCCCCEEEEEEEECHHHHHCEEEEEEEECCCCCCEEEEEEC
LKMGACAVLSETKNRSEHGQVTIKSGVPVFAVYKPAAILSALAYRFFFRKIKLHKVIAVT
CCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHEEEEEEEEE
GTNGKTTIASLLADSFMLLGKQSAQMGTIGNGLFGQLKSSLNTTLDAISVFSELHCYQQQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEYTIMEVSSHGLDQGRVSAVPFFSAIFTNLTRDHLDYHGSMENYHLAKKSLFEKYPLK
CCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
QRIINADDPIGAQWLTDFPDAISYGFNAKALTDKQSYLRVTDVDYHAQGVTFAFHSSWGK
HHHCCCCCCCCCHHHHCCCHHHHCCCCCCEECCCCCEEEEECCCCCCCCEEEEEECCCCC
GVVSAPFYGDFNVSNLAAVLTQLLVENVSIEHVQTIFPKLSTVPGRMEQYYFAEKNITFV
CCEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHEEECCCEEEE
VDYAHTPDALEKVLLALQKHKSSGRLICVFGCGGDRDTGKRPQMANIAESYAQKVVLVDD
EEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCEEEEECC
NPRTENAQSIIEDILQGFARPDRVEVIHDRRQAVNFLLNSAQSDDIVLLAGKGHEDYQIF
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEE
GTQRVHYSDRECLASLQKSADLVDYSAHK
EEEEECCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MHNDSQIMLLDDLIKPWLSDYDPSIRVSGLTLDSRQICRGYLFIALQGAQHDGRLFIEKS
CCCCCEEEEHHHHHHHHHHCCCCCEEEEEEEECHHHHHCEEEEEEEECCCCCCEEEEEEC
LKMGACAVLSETKNRSEHGQVTIKSGVPVFAVYKPAAILSALAYRFFFRKIKLHKVIAVT
CCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHEEEEEEEEE
GTNGKTTIASLLADSFMLLGKQSAQMGTIGNGLFGQLKSSLNTTLDAISVFSELHCYQQQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEYTIMEVSSHGLDQGRVSAVPFFSAIFTNLTRDHLDYHGSMENYHLAKKSLFEKYPLK
CCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
QRIINADDPIGAQWLTDFPDAISYGFNAKALTDKQSYLRVTDVDYHAQGVTFAFHSSWGK
HHHCCCCCCCCCHHHHCCCHHHHCCCCCCEECCCCCEEEEECCCCCCCCEEEEEECCCCC
GVVSAPFYGDFNVSNLAAVLTQLLVENVSIEHVQTIFPKLSTVPGRMEQYYFAEKNITFV
CCEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHEEECCCEEEE
VDYAHTPDALEKVLLALQKHKSSGRLICVFGCGGDRDTGKRPQMANIAESYAQKVVLVDD
EEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCEEEEECC
NPRTENAQSIIEDILQGFARPDRVEVIHDRRQAVNFLLNSAQSDDIVLLAGKGHEDYQIF
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEE
GTQRVHYSDRECLASLQKSADLVDYSAHK
EEEEECCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]