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The map label for this gene is grpE

Identifier: 119944677

GI number: 119944677

Start: 1146899

End: 1147519

Strand: Direct

Name: grpE

Synonym: Ping_0916

Alternate gene names: 119944677

Gene position: 1146899-1147519 (Clockwise)

Preceding gene: 119944668

Following gene: 119944678

Centisome position: 25.15

GC content: 41.87

Gene sequence:

>621_bases
ATGACGGAAGACAAAAAAACTATTCCAGAAGAACAAGTTGAAGCTGAGCAAGTTGTAGTAGAAGAAGTTGAAGCCGAGCT
AGTTGAAAATGATGAATCTGCACAACCACAAGAAGAGGCTGAAGCCTCTAACGAAGACTTAAATATGATTGAAGTACTGA
ATAAAAAATTAGCACTTGCAGAGCAACAGATTGTCGATCAGCAGGCTGATGTTGCCCGTGCCCAAGCTGATGTGGTAAAT
GCACGTCGGATTGCCGCTCAAGATGTTCAGAAAGCACACAAATTTGCACTGGTTAAATTTGCTGATGGTTTATTACCCGT
GATCGACAGTTTAGAAATGGCGATTAGCCACGCCGACAAAGAAGATGAAACGCTAAAACCTATGATTGAAGGCGTTGAAT
TAACACTAAAATCTATGCTTGATACAGTCGACAAGTTTGGCTTAAAAGTGATTGATCCAAAAGATGAAGCTTTTGACCCT
GAAAAACACCAAGCGATGAGCATGCGCGCAGTCCCTGATGTAGCGCCTAATCAAGTTATTGCTGTTATGCAAAAAGGGTA
TGAATTAAATGGCCGTGTTATTCGTCCGGCAATGGTCATGGTGTCTAAGGCTGAGGACTAA

Upstream 100 bases:

>100_bases
ATAATTTATTCATATCCTCACAACAGGCTTGAAACTATAATTGTAATCCCCATAATAGAGTATATAAAAATTTAAATCTT
TAATTGATTAGGATAAAAGC

Downstream 100 bases:

>100_bases
TTATACTTTTTTGGCTGGTCAATGAAAAGTGCTGCAAAGCGCTTTTTCACGGGTAATAAAAAATAATAAAAATACATTTT
TTTTGATGCTAGCCCTTGAA

Product: co-chaperone GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTEDKKTIPEEQVEAEQVVVEEVEAELVENDESAQPQEEAEASNEDLNMIEVLNKKLALAEQQIVDQQADVARAQADVVN
ARRIAAQDVQKAHKFALVKFADGLLPVIDSLEMAISHADKEDETLKPMIEGVELTLKSMLDTVDKFGLKVIDPKDEAFDP
EKHQAMSMRAVPDVAPNQVIAVMQKGYELNGRVIRPAMVMVSKAED

Sequences:

>Translated_206_residues
MTEDKKTIPEEQVEAEQVVVEEVEAELVENDESAQPQEEAEASNEDLNMIEVLNKKLALAEQQIVDQQADVARAQADVVN
ARRIAAQDVQKAHKFALVKFADGLLPVIDSLEMAISHADKEDETLKPMIEGVELTLKSMLDTVDKFGLKVIDPKDEAFDP
EKHQAMSMRAVPDVAPNQVIAVMQKGYELNGRVIRPAMVMVSKAED
>Mature_205_residues
TEDKKTIPEEQVEAEQVVVEEVEAELVENDESAQPQEEAEASNEDLNMIEVLNKKLALAEQQIVDQQADVARAQADVVNA
RRIAAQDVQKAHKFALVKFADGLLPVIDSLEMAISHADKEDETLKPMIEGVELTLKSMLDTVDKFGLKVIDPKDEAFDPE
KHQAMSMRAVPDVAPNQVIAVMQKGYELNGRVIRPAMVMVSKAED

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=148, Percent_Identity=33.1081081081081, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1788967, Length=207, Percent_Identity=44.4444444444444, Blast_Score=150, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17552458, Length=138, Percent_Identity=34.7826086956522, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=177, Percent_Identity=35.0282485875706, Blast_Score=99, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653432, Length=177, Percent_Identity=34.4632768361582, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PSYIN (A1STE3)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942357.1
- ProteinModelPortal:   A1STE3
- SMR:   A1STE3
- STRING:   A1STE3
- GeneID:   4623571
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0916
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- PhylomeDB:   A1STE3
- BioCyc:   PING357804:PING_0916-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22833; Mature: 22702

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDKKTIPEEQVEAEQVVVEEVEAELVENDESAQPQEEAEASNEDLNMIEVLNKKLALA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
EQQIVDQQADVARAQADVVNARRIAAQDVQKAHKFALVKFADGLLPVIDSLEMAISHADK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
EDETLKPMIEGVELTLKSMLDTVDKFGLKVIDPKDEAFDPEKHQAMSMRAVPDVAPNQVI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHH
AVMQKGYELNGRVIRPAMVMVSKAED
HHHHCCCCCCCEEEHHHHHHHHCCCC
>Mature Secondary Structure 
TEDKKTIPEEQVEAEQVVVEEVEAELVENDESAQPQEEAEASNEDLNMIEVLNKKLALA
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
EQQIVDQQADVARAQADVVNARRIAAQDVQKAHKFALVKFADGLLPVIDSLEMAISHADK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
EDETLKPMIEGVELTLKSMLDTVDKFGLKVIDPKDEAFDPEKHQAMSMRAVPDVAPNQVI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHH
AVMQKGYELNGRVIRPAMVMVSKAED
HHHHCCCCCCCEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA