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The map label for this gene is speE [H]

Identifier: 119944667

GI number: 119944667

Start: 1135349

End: 1137061

Strand: Direct

Name: speE [H]

Synonym: Ping_0906

Alternate gene names: 119944667

Gene position: 1135349-1137061 (Clockwise)

Preceding gene: 119944666

Following gene: 119944668

Centisome position: 24.9

GC content: 39.75

Gene sequence:

>1713_bases
ATGGTCCAGGCAAAAAAAATAAAACTGCTTATCGATGACAGCCTGCTGATATTAATCATGGCAGTACTGGCTTGCTGTGG
TCTGATTTATGAGTATTTACTTTCTCATTATTCAGCACGTATTCTTGGTAGTGTTGAAACGGTTATTTATGCGATTATTG
GTATTATGATTGTATCAATGGGGTTAGGCGCCTTTGCCGCCAAAAAAGTTTCCAATGCCTTTCAAGGTTTTGTTATTTTA
GAATTAGCCGTTGCTTTAATCGGTTGTTGTGCGACTCTGTTTATCGCCTCGATCATCGGGTTTGGGCAGACATTACCGCA
TCTGTTGGCTGAAACCTATCAAATCCCGATGGACACACGACCAGAGGGAGGAATGATTGCCCAACTCAACTGGTTGAGCA
TACAATTACCTTACTTTTTTGCATTTCTTCTTGGCTTTTTGATTGGCATGGAAATACCGCTGATTGCGCGAATTCGTGAA
ACCGTATACGGGCATCATTTAGCCCATAATGCGGGGACTATTTACGGGGCTGATTATATCGGAGCGGGTTTTGGTGCCGC
GATTTGGGTATTGTTTATGCTGCGTATTGAAATCAGCCAGGCTGCAGCACTCACCGCTTCTCTCAATTTAGTGGCAGGGT
TTGTTTTTCTGCTACGCTTTAAGCACTACTTAAGGCACATTAAAAAACTATTATTAGGGCATGGCTTGTTAGCTATTTTA
GTGCTTTTGGTTTTCCAATATGGCGGCAGTTGGCAACAGAAAATGCAAAACCTTTTGTATCAAGATAAGGTGGTTTACCA
AACCCAAACACCTTATCAAAATTTAGTGTTTACGCAGCGCAATTTAGGTGGAAATCTTGAGCCCATTTTCAACTTTTATA
TTAATGGGCGCTTACAATTCTCCAGCCTGGATGAAAAAATTTACCATGAGTTTTTAGTTCATCCTGCAATGCAGGCGGCG
GCACGGCAAAATAAGATATTGATTATTGGTGGGGGTGACGGTTTGGCATTGCGCGAGGTCTTAAAATGGCAGCCTGAGAA
GGTCACTTTAATTGATTTGGATGCGCAGTTAGTTGAATTGTTTAAATCTCCTCAAGCTGTATTACCCGCTTATTTAGCAA
AGAAAATTAGCGCGTTAACCGAAAACAGCTTTAATGATAAACGTGTCACTGTTATTAATGATGATGCATTTATAACCATT
GATAAGTTATTACAGCGCAAAAAAATGTTTGATGTGATTATTATTGACCTGCCGGATCCAAGCCACCCTGATTTAAATAA
GCTCTACAGTGTTAATTTTTATTACCGGCTCAATAATTTGTTAAATGGGGATGGCGTGATAGCGATCCAGTCTACATCGC
CCTTTCATGCTAAAAATGCTTTTATCTCAATTGCTAAAACTCTAAAAGAAGCTAAATTTCCCTTTGTGGAACAATACCAT
CAAAATGTGCCTTCATTTGGTGAGTGGGGCTGGACCATTTCTACAAAATTGGGTGCCAGTGTCAGTAAACGTTTACAAAA
TGCACCATCATTGTCAGTGCCAACAAGCTGGTTAACTCTGCCTTTATTAAAAAGTGCTTTTGTTTTTAATAAAGGTTTTT
ATGATGATATCAATAGTATTAAAGTAAATTATTTAGGATCTAATCAACTCTACCGATACCATCAGCGAGCATGGGAAAGA
GAGACTGAGTTGCCACCGTCAAATGCCGATTGA

Upstream 100 bases:

>100_bases
ATGGCCTGAGCAACGAACCGACAGCTGACAGCTTTCAGCTAAGCGCTTATTTAATTGGTCATCTTTTGATGGCCTTTTTT
TATCCCCGTTAGAGAAAAAT

Downstream 100 bases:

>100_bases
TGGCATGGATAACAGACACTTATTTAACATGGAGCAGCGAGCATCAAGCGCTCTATTGACCTGAATATTAGCGTTAAATT
AAGTAAGATTAGGAAATAAA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MVQAKKIKLLIDDSLLILIMAVLACCGLIYEYLLSHYSARILGSVETVIYAIIGIMIVSMGLGAFAAKKVSNAFQGFVIL
ELAVALIGCCATLFIASIIGFGQTLPHLLAETYQIPMDTRPEGGMIAQLNWLSIQLPYFFAFLLGFLIGMEIPLIARIRE
TVYGHHLAHNAGTIYGADYIGAGFGAAIWVLFMLRIEISQAAALTASLNLVAGFVFLLRFKHYLRHIKKLLLGHGLLAIL
VLLVFQYGGSWQQKMQNLLYQDKVVYQTQTPYQNLVFTQRNLGGNLEPIFNFYINGRLQFSSLDEKIYHEFLVHPAMQAA
ARQNKILIIGGGDGLALREVLKWQPEKVTLIDLDAQLVELFKSPQAVLPAYLAKKISALTENSFNDKRVTVINDDAFITI
DKLLQRKKMFDVIIIDLPDPSHPDLNKLYSVNFYYRLNNLLNGDGVIAIQSTSPFHAKNAFISIAKTLKEAKFPFVEQYH
QNVPSFGEWGWTISTKLGASVSKRLQNAPSLSVPTSWLTLPLLKSAFVFNKGFYDDINSIKVNYLGSNQLYRYHQRAWER
ETELPPSNAD

Sequences:

>Translated_570_residues
MVQAKKIKLLIDDSLLILIMAVLACCGLIYEYLLSHYSARILGSVETVIYAIIGIMIVSMGLGAFAAKKVSNAFQGFVIL
ELAVALIGCCATLFIASIIGFGQTLPHLLAETYQIPMDTRPEGGMIAQLNWLSIQLPYFFAFLLGFLIGMEIPLIARIRE
TVYGHHLAHNAGTIYGADYIGAGFGAAIWVLFMLRIEISQAAALTASLNLVAGFVFLLRFKHYLRHIKKLLLGHGLLAIL
VLLVFQYGGSWQQKMQNLLYQDKVVYQTQTPYQNLVFTQRNLGGNLEPIFNFYINGRLQFSSLDEKIYHEFLVHPAMQAA
ARQNKILIIGGGDGLALREVLKWQPEKVTLIDLDAQLVELFKSPQAVLPAYLAKKISALTENSFNDKRVTVINDDAFITI
DKLLQRKKMFDVIIIDLPDPSHPDLNKLYSVNFYYRLNNLLNGDGVIAIQSTSPFHAKNAFISIAKTLKEAKFPFVEQYH
QNVPSFGEWGWTISTKLGASVSKRLQNAPSLSVPTSWLTLPLLKSAFVFNKGFYDDINSIKVNYLGSNQLYRYHQRAWER
ETELPPSNAD
>Mature_570_residues
MVQAKKIKLLIDDSLLILIMAVLACCGLIYEYLLSHYSARILGSVETVIYAIIGIMIVSMGLGAFAAKKVSNAFQGFVIL
ELAVALIGCCATLFIASIIGFGQTLPHLLAETYQIPMDTRPEGGMIAQLNWLSIQLPYFFAFLLGFLIGMEIPLIARIRE
TVYGHHLAHNAGTIYGADYIGAGFGAAIWVLFMLRIEISQAAALTASLNLVAGFVFLLRFKHYLRHIKKLLLGHGLLAIL
VLLVFQYGGSWQQKMQNLLYQDKVVYQTQTPYQNLVFTQRNLGGNLEPIFNFYINGRLQFSSLDEKIYHEFLVHPAMQAA
ARQNKILIIGGGDGLALREVLKWQPEKVTLIDLDAQLVELFKSPQAVLPAYLAKKISALTENSFNDKRVTVINDDAFITI
DKLLQRKKMFDVIIIDLPDPSHPDLNKLYSVNFYYRLNNLLNGDGVIAIQSTSPFHAKNAFISIAKTLKEAKFPFVEQYH
QNVPSFGEWGWTISTKLGASVSKRLQNAPSLSVPTSWLTLPLLKSAFVFNKGFYDDINSIKVNYLGSNQLYRYHQRAWER
ETELPPSNAD

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=238, Percent_Identity=24.3697478991597, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786312, Length=241, Percent_Identity=28.2157676348548, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI212645616, Length=292, Percent_Identity=26.7123287671233, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25154106, Length=209, Percent_Identity=30.622009569378, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323175, Length=260, Percent_Identity=28.4615384615385, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325326, Length=241, Percent_Identity=29.045643153527, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24645443, Length=193, Percent_Identity=27.979274611399, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 63895; Mature: 63895

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQAKKIKLLIDDSLLILIMAVLACCGLIYEYLLSHYSARILGSVETVIYAIIGIMIVSM
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGAFAAKKVSNAFQGFVILELAVALIGCCATLFIASIIGFGQTLPHLLAETYQIPMDTR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PEGGMIAQLNWLSIQLPYFFAFLLGFLIGMEIPLIARIRETVYGHHLAHNAGTIYGADYI
CCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEECCHHH
GAGFGAAIWVLFMLRIEISQAAALTASLNLVAGFVFLLRFKHYLRHIKKLLLGHGLLAIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VLLVFQYGGSWQQKMQNLLYQDKVVYQTQTPYQNLVFTQRNLGGNLEPIFNFYINGRLQF
HHHHHHCCCCHHHHHHHHHHHCCEEEEECCCHHHHEEEECCCCCCCCHHEEEEECCEEEE
SSLDEKIYHEFLVHPAMQAAARQNKILIIGGGDGLALREVLKWQPEKVTLIDLDAQLVEL
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEEEEECCHHHHHH
FKSPQAVLPAYLAKKISALTENSFNDKRVTVINDDAFITIDKLLQRKKMFDVIIIDLPDP
HCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHCEEEEEEECCCC
SHPDLNKLYSVNFYYRLNNLLNGDGVIAIQSTSPFHAKNAFISIAKTLKEAKFPFVEQYH
CCCCCHHHEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
QNVPSFGEWGWTISTKLGASVSKRLQNAPSLSVPTSWLTLPLLKSAFVFNKGFYDDINSI
CCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCE
KVNYLGSNQLYRYHQRAWERETELPPSNAD
EEEEECCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVQAKKIKLLIDDSLLILIMAVLACCGLIYEYLLSHYSARILGSVETVIYAIIGIMIVSM
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGAFAAKKVSNAFQGFVILELAVALIGCCATLFIASIIGFGQTLPHLLAETYQIPMDTR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PEGGMIAQLNWLSIQLPYFFAFLLGFLIGMEIPLIARIRETVYGHHLAHNAGTIYGADYI
CCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEECCHHH
GAGFGAAIWVLFMLRIEISQAAALTASLNLVAGFVFLLRFKHYLRHIKKLLLGHGLLAIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VLLVFQYGGSWQQKMQNLLYQDKVVYQTQTPYQNLVFTQRNLGGNLEPIFNFYINGRLQF
HHHHHHCCCCHHHHHHHHHHHCCEEEEECCCHHHHEEEECCCCCCCCHHEEEEECCEEEE
SSLDEKIYHEFLVHPAMQAAARQNKILIIGGGDGLALREVLKWQPEKVTLIDLDAQLVEL
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEEEEECCHHHHHH
FKSPQAVLPAYLAKKISALTENSFNDKRVTVINDDAFITIDKLLQRKKMFDVIIIDLPDP
HCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHCEEEEEEECCCC
SHPDLNKLYSVNFYYRLNNLLNGDGVIAIQSTSPFHAKNAFISIAKTLKEAKFPFVEQYH
CCCCCHHHEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
QNVPSFGEWGWTISTKLGASVSKRLQNAPSLSVPTSWLTLPLLKSAFVFNKGFYDDINSI
CCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCE
KVNYLGSNQLYRYHQRAWERETELPPSNAD
EEEEECCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]