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The map label for this gene is glgX [H]

Identifier: 119944654

GI number: 119944654

Start: 1117964

End: 1120045

Strand: Direct

Name: glgX [H]

Synonym: Ping_0893

Alternate gene names: 119944654

Gene position: 1117964-1120045 (Clockwise)

Preceding gene: 119944653

Following gene: 119944656

Centisome position: 24.52

GC content: 41.07

Gene sequence:

>2082_bases
ATGTATGAATTACTGCCAGGTAAAACCCATCCTATCGGTGCCACGGTGGAGGATGGGGGGGTTAATTTTTGCCTCTATTG
TGATGAACGATCAACCTCCGTCGAGCTGCTTCTGTTTGAACAGCATAATGCGCTTGAACCTTACGAGATTATCCGGTTCG
ATCCCCTGATTCATCGTAGCTTTTATTTTTGGCATATTTTTGTAAAGGGTTTACCGGAAAAAACACATTATGCTTACCGT
GTTGGTGGCCCTTTTCAACCTGAAAACGGGCTGGTTTTTGATCCGCAAAAAGTATTAATTGATCCCTACTCAAAAGGTAT
TAATTGTAGTTTATGGCAGCGAAATGATGCCTGCAAAGCGGGTGATAATCTGCACACTTCGATGCGCAGTTGCGTACTCA
AGCTAACGGATTATGACTGGCAGGGTGATAGCCCTCCTAATCATCATCTCAATGAAAGTATTATTTATGAGATGAATGTC
GGCGGATTTACTAAATCACCGACCTCCGGGGTAAAACATCCGGGTACCTATGCAGGCATAATAGAAAAAATACCCTATTT
AACAGCATTAGGTATTACGGCGGTAGAGTTGTTACCCATATTTGAATTTGAAGACTCTGAGGTGCGTTATTTTAATGGCA
ATAAATTAGTTAATTATTGGGGATATAGCACCATTAATTTTTTTGCACCGCATTCGGGGTACTGTGTCAATCCACAGGAA
GGTAACCACCTTGATGAATTTAGAGATCTCGTTAAGGCGCTACACCAGGCTGATATTAGTGTGATTCTGGATGTTGTTTT
TAACCACACCGATGAAAGTGATCATCGAGGGCCAGTTTACTCTTTTAAAGGTTTTGCTAATAATAGCTATTATTTTTTAA
TGGATAAGCATAAAAATTTTTACTGTAATTATTCTGGATGCGGTAATACTTTTAAATGCAACCATCCTATTGGTGAAAAA
TTTATTGTTGATTGTTTAGAATATTGGGTGCGGGATATGCATGTCGATGGTTTTCGTTTTGACGAAGGCACTATCTTAGC
ACGTGGGGATAACGGTAATTTAATTGCCAATCCTCCCGTTGTTTGGGCTATCGAATTATCTGAACAATTAGCCGATACTA
AGGTGATTGCAGAAGCTTGGGATGCGGAGGGAGGATATCTGGTGGGATCTTTCCCCGGACATCGTTGGGCGGAGTGGAAC
GGTCTTTATCGAGATGATCTGCGTGATTTTGTGCGCGGTAAAGCGGGTATGCTGGGCACGTTTGCCCGGCGTATTACCGG
CAGTGCCGATATCTACGAGTCCCAATATGAATTACCCGGTAACAGTATTAATTTTATTAATTCCCATGATGGTTTTACCC
TTAATGATCTTGTGTCCTATAACCATAAACATAATCAGGCGAATGGGGAAAATAATAGTGATGGTATTGATAATAACAGA
AGTTGGAATTGTGGTGTTGAGGGTATAACCGATAATCCACAAATCAATATGCTGCGCAAGCGACAAATCAAAAATTTTGC
CGCTATTTTAATGTTATCCAAAGGTGTCCCGATGTTTGTTGCAGGCGATGAAATTTGCAGAACACAACAGGGAAACAATA
ATGCTTATTGTCAGGATAACGAACTATCCTGGTTCAATTGGCAGGATGTTGATACCAATAAGTCTATGCTGCAGTTCTGG
CAACGGCTGATCGCTTTTCGTAAAGTTCACCCGCGTTTATTTCGCAATAAATATTACAATAGTGAAATAAACAAGAGTGG
ATTATCCGATATATTATGGCATGGATGTGAACTGGGGAAACCTGGTTGGTATGACCCAAGCGGGCTGGCATTAGCGATGA
CACTTGGAGAACGGGCCGACGGGGAAGATATACATATTATGTTTAATATGTATTGGGAAGGACTTGAATTTGAGTTGCCG
GATATTGAAGGAGAGAAGTGGTATCGCGCCATTGATACATTCTTATCCTCTCCTTTAGATATCGCTAAAACCGGGGAGGA
AATAGTGCATAGCGGTTCGCATTATAAAGTGAATGCGCGCAGTGTTGTTGTCTTAATTTCAAAAAAAGATCCGATCTGCT
AA

Upstream 100 bases:

>100_bases
TGATCAGTATCCTAATCCTTGTTGCGCAATAGCGCCATTGATTAATACCCCTTTTTGATTAAAGTTGGTCATAACACTTT
GGTTACATAAAGAGTCCCGA

Downstream 100 bases:

>100_bases
GCCCAATCTTTTTTATTCTGACAGGCGGCAATAAAGGCTGAAAAAAACACACAGGGTGACAGCCTTTTTCACACTCCTTT
AAGACCAACCCTTTAGGTTA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MYELLPGKTHPIGATVEDGGVNFCLYCDERSTSVELLLFEQHNALEPYEIIRFDPLIHRSFYFWHIFVKGLPEKTHYAYR
VGGPFQPENGLVFDPQKVLIDPYSKGINCSLWQRNDACKAGDNLHTSMRSCVLKLTDYDWQGDSPPNHHLNESIIYEMNV
GGFTKSPTSGVKHPGTYAGIIEKIPYLTALGITAVELLPIFEFEDSEVRYFNGNKLVNYWGYSTINFFAPHSGYCVNPQE
GNHLDEFRDLVKALHQADISVILDVVFNHTDESDHRGPVYSFKGFANNSYYFLMDKHKNFYCNYSGCGNTFKCNHPIGEK
FIVDCLEYWVRDMHVDGFRFDEGTILARGDNGNLIANPPVVWAIELSEQLADTKVIAEAWDAEGGYLVGSFPGHRWAEWN
GLYRDDLRDFVRGKAGMLGTFARRITGSADIYESQYELPGNSINFINSHDGFTLNDLVSYNHKHNQANGENNSDGIDNNR
SWNCGVEGITDNPQINMLRKRQIKNFAAILMLSKGVPMFVAGDEICRTQQGNNNAYCQDNELSWFNWQDVDTNKSMLQFW
QRLIAFRKVHPRLFRNKYYNSEINKSGLSDILWHGCELGKPGWYDPSGLALAMTLGERADGEDIHIMFNMYWEGLEFELP
DIEGEKWYRAIDTFLSSPLDIAKTGEEIVHSGSHYKVNARSVVVLISKKDPIC

Sequences:

>Translated_693_residues
MYELLPGKTHPIGATVEDGGVNFCLYCDERSTSVELLLFEQHNALEPYEIIRFDPLIHRSFYFWHIFVKGLPEKTHYAYR
VGGPFQPENGLVFDPQKVLIDPYSKGINCSLWQRNDACKAGDNLHTSMRSCVLKLTDYDWQGDSPPNHHLNESIIYEMNV
GGFTKSPTSGVKHPGTYAGIIEKIPYLTALGITAVELLPIFEFEDSEVRYFNGNKLVNYWGYSTINFFAPHSGYCVNPQE
GNHLDEFRDLVKALHQADISVILDVVFNHTDESDHRGPVYSFKGFANNSYYFLMDKHKNFYCNYSGCGNTFKCNHPIGEK
FIVDCLEYWVRDMHVDGFRFDEGTILARGDNGNLIANPPVVWAIELSEQLADTKVIAEAWDAEGGYLVGSFPGHRWAEWN
GLYRDDLRDFVRGKAGMLGTFARRITGSADIYESQYELPGNSINFINSHDGFTLNDLVSYNHKHNQANGENNSDGIDNNR
SWNCGVEGITDNPQINMLRKRQIKNFAAILMLSKGVPMFVAGDEICRTQQGNNNAYCQDNELSWFNWQDVDTNKSMLQFW
QRLIAFRKVHPRLFRNKYYNSEINKSGLSDILWHGCELGKPGWYDPSGLALAMTLGERADGEDIHIMFNMYWEGLEFELP
DIEGEKWYRAIDTFLSSPLDIAKTGEEIVHSGSHYKVNARSVVVLISKKDPIC
>Mature_693_residues
MYELLPGKTHPIGATVEDGGVNFCLYCDERSTSVELLLFEQHNALEPYEIIRFDPLIHRSFYFWHIFVKGLPEKTHYAYR
VGGPFQPENGLVFDPQKVLIDPYSKGINCSLWQRNDACKAGDNLHTSMRSCVLKLTDYDWQGDSPPNHHLNESIIYEMNV
GGFTKSPTSGVKHPGTYAGIIEKIPYLTALGITAVELLPIFEFEDSEVRYFNGNKLVNYWGYSTINFFAPHSGYCVNPQE
GNHLDEFRDLVKALHQADISVILDVVFNHTDESDHRGPVYSFKGFANNSYYFLMDKHKNFYCNYSGCGNTFKCNHPIGEK
FIVDCLEYWVRDMHVDGFRFDEGTILARGDNGNLIANPPVVWAIELSEQLADTKVIAEAWDAEGGYLVGSFPGHRWAEWN
GLYRDDLRDFVRGKAGMLGTFARRITGSADIYESQYELPGNSINFINSHDGFTLNDLVSYNHKHNQANGENNSDGIDNNR
SWNCGVEGITDNPQINMLRKRQIKNFAAILMLSKGVPMFVAGDEICRTQQGNNNAYCQDNELSWFNWQDVDTNKSMLQFW
QRLIAFRKVHPRLFRNKYYNSEINKSGLSDILWHGCELGKPGWYDPSGLALAMTLGERADGEDIHIMFNMYWEGLEFELP
DIEGEKWYRAIDTFLSSPLDIAKTGEEIVHSGSHYKVNARSVVVLISKKDPIC

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=590, Percent_Identity=40.6779661016949, Blast_Score=400, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78999; Mature: 78999

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYELLPGKTHPIGATVEDGGVNFCLYCDERSTSVELLLFEQHNALEPYEIIRFDPLIHRS
CCCCCCCCCCCCCCEEECCCEEEEEEECCCCCEEEEEEEECCCCCCCHHEEEECHHHHHH
FYFWHIFVKGLPEKTHYAYRVGGPFQPENGLVFDPQKVLIDPYSKGINCSLWQRNDACKA
EEEEEEEEECCCCCCCEEEEECCCCCCCCCEEECCHHEEECCCCCCCEEEEECCCCCCCC
GDNLHTSMRSCVLKLTDYDWQGDSPPNHHLNESIIYEMNVGGFTKSPTSGVKHPGTYAGI
CCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
IEKIPYLTALGITAVELLPIFEFEDSEVRYFNGNKLVNYWGYSTINFFAPHSGYCVNPQE
HHHCCHHHHHHHHHEEEEEEEECCCCCEEEECCCEEEEEECCCEEEEECCCCCEECCCCC
GNHLDEFRDLVKALHQADISVILDVVFNHTDESDHRGPVYSFKGFANNSYYFLMDKHKNF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCE
YCNYSGCGNTFKCNHPIGEKFIVDCLEYWVRDMHVDGFRFDEGTILARGDNGNLIANPPV
EEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCEEECCCE
VWAIELSEQLADTKVIAEAWDAEGGYLVGSFPGHRWAEWNGLYRDDLRDFVRGKAGMLGT
EEEEECHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHH
FARRITGSADIYESQYELPGNSINFINSHDGFTLNDLVSYNHKHNQANGENNSDGIDNNR
HHHHHCCCCHHCCCEECCCCCCEEEEECCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCCC
SWNCGVEGITDNPQINMLRKRQIKNFAAILMLSKGVPMFVAGDEICRTQQGNNNAYCQDN
CCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCEEEECHHHHHCCCCCCCCEEECC
ELSWFNWQDVDTNKSMLQFWQRLIAFRKVHPRLFRNKYYNSEINKSGLSDILWHGCELGK
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
PGWYDPSGLALAMTLGERADGEDIHIMFNMYWEGLEFELPDIEGEKWYRAIDTFLSSPLD
CCCCCCCCCEEEEEECCCCCCCEEEEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCC
IAKTGEEIVHSGSHYKVNARSVVVLISKKDPIC
HHHCCHHHHHCCCCEEEECCEEEEEEECCCCCC
>Mature Secondary Structure
MYELLPGKTHPIGATVEDGGVNFCLYCDERSTSVELLLFEQHNALEPYEIIRFDPLIHRS
CCCCCCCCCCCCCCEEECCCEEEEEEECCCCCEEEEEEEECCCCCCCHHEEEECHHHHHH
FYFWHIFVKGLPEKTHYAYRVGGPFQPENGLVFDPQKVLIDPYSKGINCSLWQRNDACKA
EEEEEEEEECCCCCCCEEEEECCCCCCCCCEEECCHHEEECCCCCCCEEEEECCCCCCCC
GDNLHTSMRSCVLKLTDYDWQGDSPPNHHLNESIIYEMNVGGFTKSPTSGVKHPGTYAGI
CCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
IEKIPYLTALGITAVELLPIFEFEDSEVRYFNGNKLVNYWGYSTINFFAPHSGYCVNPQE
HHHCCHHHHHHHHHEEEEEEEECCCCCEEEECCCEEEEEECCCEEEEECCCCCEECCCCC
GNHLDEFRDLVKALHQADISVILDVVFNHTDESDHRGPVYSFKGFANNSYYFLMDKHKNF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCE
YCNYSGCGNTFKCNHPIGEKFIVDCLEYWVRDMHVDGFRFDEGTILARGDNGNLIANPPV
EEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCEEECCCE
VWAIELSEQLADTKVIAEAWDAEGGYLVGSFPGHRWAEWNGLYRDDLRDFVRGKAGMLGT
EEEEECHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHH
FARRITGSADIYESQYELPGNSINFINSHDGFTLNDLVSYNHKHNQANGENNSDGIDNNR
HHHHHCCCCHHCCCEECCCCCCEEEEECCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCCC
SWNCGVEGITDNPQINMLRKRQIKNFAAILMLSKGVPMFVAGDEICRTQQGNNNAYCQDN
CCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCEEEECHHHHHCCCCCCCCEEECC
ELSWFNWQDVDTNKSMLQFWQRLIAFRKVHPRLFRNKYYNSEINKSGLSDILWHGCELGK
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
PGWYDPSGLALAMTLGERADGEDIHIMFNMYWEGLEFELPDIEGEKWYRAIDTFLSSPLD
CCCCCCCCCEEEEEECCCCCCCEEEEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCC
IAKTGEEIVHSGSHYKVNARSVVVLISKKDPIC
HHHCCHHHHHCCCCEEEECCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]