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The map label for this gene is truB

Identifier: 119944581

GI number: 119944581

Start: 1023594

End: 1024526

Strand: Direct

Name: truB

Synonym: Ping_0819

Alternate gene names: 119944581

Gene position: 1023594-1024526 (Clockwise)

Preceding gene: 119944580

Following gene: 119944582

Centisome position: 22.45

GC content: 43.73

Gene sequence:

>933_bases
ATGGCAAGACGGGGAAAGGGGCGTTCAATAAACGGTATCATTTTACTGGATAAACCCACAGACATCAGCTCAAACCATGC
ATTACAACGTGTTAAGCGTATTTTCTTTGCTAATAAAGCGGGGCATACGGGTGCGTTAGATCCACTTGCTACGGGAATGC
TGCCGGTTTGTTTAGGGGAAGCAACCAAATTTTCTCAGTTTTTGCTCGATTCCGATAAACGTTATATTGTCACTGCAACC
TTAGGTGTGCGCACAACCACCAGTGATTCACAGGGTGATGTGGTTAGCGAGCGTCCGGTTGAGGTTACTCAAACGCAGCT
TGATCAGGCACTGGACACTTTCCGCGGTGATATTATGCAGGTACCGTCGATGTTTTCTGCCTTAAAACATAAAGGCCAGC
CCCTTTATAAATATGCCCGTGAAGGTATTACTATTGAGCGTGAAGCACGACCTATTACTGTCTATGAAATTAACCAATTA
CGCTTTGAAGGTAATGAAGTAGAGTTAGATATTCACGTTAGTAAAGGGACTTATATCCGCACTATCGTTGATGATCTTGG
TGAGTTATTAGGCTGTGGCGCGCATGTGTCTGTGTTACGCCGTACGCAGGTTGCCGATTATCCATACGATAAAATGGTCA
CTATTGAACAATTAGAAGCATTGATTGAGCAAGCAAAAGAATCTGATGTCAGCCCCTATGATTTATTAGATCCGCTGTTA
CTTGATATGGATACTGCAGTAAAAAAATACCCGGAAGTTAATATCTCAGATGAGATGGGCGAGTATGTCTTGCATGGTCA
ACCGGTACAAGTTTTTGGTGCGCCTGATAATACCGTCCTTAGGATCACCGTTGGTGAGGCTCATACTTTTATTGGTGTTG
GTAAAATGAACGAGGCAGGTTTAATTGCCCCAACCCGTTTGGCGAACTTGTAA

Upstream 100 bases:

>100_bases
TAACCGAAGGTTTACGTATTTCAGAATTAGTGAGCGGAGCAGTAAAAGCTGATAAGACTAAATTAGCTGCGTCAGGCAGA
ACGGAAGAGGACGAGGACTA

Downstream 100 bases:

>100_bases
GTAAAAGAAAGCTGCGAACTTCGTAGCTGTCAGAGTGAGATAACAATCATGACAAAACTAGACTTCTCAGCAATCAATAA
ATCAGCAGCTAACTCATTTA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MARRGKGRSINGIILLDKPTDISSNHALQRVKRIFFANKAGHTGALDPLATGMLPVCLGEATKFSQFLLDSDKRYIVTAT
LGVRTTTSDSQGDVVSERPVEVTQTQLDQALDTFRGDIMQVPSMFSALKHKGQPLYKYAREGITIEREARPITVYEINQL
RFEGNEVELDIHVSKGTYIRTIVDDLGELLGCGAHVSVLRRTQVADYPYDKMVTIEQLEALIEQAKESDVSPYDLLDPLL
LDMDTAVKKYPEVNISDEMGEYVLHGQPVQVFGAPDNTVLRITVGEAHTFIGVGKMNEAGLIAPTRLANL

Sequences:

>Translated_310_residues
MARRGKGRSINGIILLDKPTDISSNHALQRVKRIFFANKAGHTGALDPLATGMLPVCLGEATKFSQFLLDSDKRYIVTAT
LGVRTTTSDSQGDVVSERPVEVTQTQLDQALDTFRGDIMQVPSMFSALKHKGQPLYKYAREGITIEREARPITVYEINQL
RFEGNEVELDIHVSKGTYIRTIVDDLGELLGCGAHVSVLRRTQVADYPYDKMVTIEQLEALIEQAKESDVSPYDLLDPLL
LDMDTAVKKYPEVNISDEMGEYVLHGQPVQVFGAPDNTVLRITVGEAHTFIGVGKMNEAGLIAPTRLANL
>Mature_309_residues
ARRGKGRSINGIILLDKPTDISSNHALQRVKRIFFANKAGHTGALDPLATGMLPVCLGEATKFSQFLLDSDKRYIVTATL
GVRTTTSDSQGDVVSERPVEVTQTQLDQALDTFRGDIMQVPSMFSALKHKGQPLYKYAREGITIEREARPITVYEINQLR
FEGNEVELDIHVSKGTYIRTIVDDLGELLGCGAHVSVLRRTQVADYPYDKMVTIEQLEALIEQAKESDVSPYDLLDPLLL
DMDTAVKKYPEVNISDEMGEYVLHGQPVQVFGAPDNTVLRITVGEAHTFIGVGKMNEAGLIAPTRLANL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=286, Percent_Identity=33.5664335664336, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4503337, Length=176, Percent_Identity=30.1136363636364, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI215599015, Length=176, Percent_Identity=30.1136363636364, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI2367200, Length=308, Percent_Identity=61.3636363636364, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17553978, Length=195, Percent_Identity=30.2564102564103, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=304, Percent_Identity=27.6315789473684, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=189, Percent_Identity=31.7460317460317, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364189, Length=232, Percent_Identity=28.0172413793103, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364187, Length=232, Percent_Identity=28.0172413793103, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364185, Length=232, Percent_Identity=28.0172413793103, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364183, Length=232, Percent_Identity=28.0172413793103, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62471759, Length=232, Percent_Identity=28.0172413793103, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17975520, Length=232, Percent_Identity=28.0172413793103, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PSYIN (A1ST47)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942261.1
- ProteinModelPortal:   A1ST47
- SMR:   A1ST47
- STRING:   A1ST47
- GeneID:   4625460
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0819
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   A1ST47
- BioCyc:   PING357804:PING_0819-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34246; Mature: 34114

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRGKGRSINGIILLDKPTDISSNHALQRVKRIFFANKAGHTGALDPLATGMLPVCLGE
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYIVTATLGVRTTTSDSQGDVVSERPVEVTQTQLDQALDTFRGDIMQ
HHHHHHHHHCCCCCEEEEEEECEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VPSMFSALKHKGQPLYKYAREGITIEREARPITVYEINQLRFEGNEVELDIHVSKGTYIR
HHHHHHHHHHCCCHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIVDDLGELLGCGAHVSVLRRTQVADYPYDKMVTIEQLEALIEQAKESDVSPYDLLDPLL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHH
LDMDTAVKKYPEVNISDEMGEYVLHGQPVQVFGAPDNTVLRITVGEAHTFIGVGKMNEAG
HHHHHHHHHCCCCCCCHHHCCCEECCCCEEEEECCCCEEEEEEECCCEEEEEECCCCCCC
LIAPTRLANL
CCCCHHHCCC
>Mature Secondary Structure 
ARRGKGRSINGIILLDKPTDISSNHALQRVKRIFFANKAGHTGALDPLATGMLPVCLGE
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYIVTATLGVRTTTSDSQGDVVSERPVEVTQTQLDQALDTFRGDIMQ
HHHHHHHHHCCCCCEEEEEEECEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VPSMFSALKHKGQPLYKYAREGITIEREARPITVYEINQLRFEGNEVELDIHVSKGTYIR
HHHHHHHHHHCCCHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIVDDLGELLGCGAHVSVLRRTQVADYPYDKMVTIEQLEALIEQAKESDVSPYDLLDPLL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHH
LDMDTAVKKYPEVNISDEMGEYVLHGQPVQVFGAPDNTVLRITVGEAHTFIGVGKMNEAG
HHHHHHHHHCCCCCCCHHHCCCEECCCCEEEEECCCCEEEEEEECCCEEEEEECCCCCCC
LIAPTRLANL
CCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA