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The map label for this gene is manB [H]

Identifier: 119944535

GI number: 119944535

Start: 967497

End: 968924

Strand: Direct

Name: manB [H]

Synonym: Ping_0769

Alternate gene names: 119944535

Gene position: 967497-968924 (Clockwise)

Preceding gene: 119944534

Following gene: 119944536

Centisome position: 21.22

GC content: 40.9

Gene sequence:

>1428_bases
ATGAACAAAAAATTAATTAGCGCAACGGTTATCGCAGAAAGTGGTATTGCATTTGGCACTAGTGGTGCACGTGGTTTAGT
GACTCAACTCACCCCTGATGTCTGTGCCGCCTTTGCCGTTGCATTTATTGCGGGTATGACGCGCAACTTTAATTTTAAGC
AAGTTGCGATTGCAATTGACAACCGCCCGAGCAGTTATGCGATGGCAAAAGCTTGTGCCGCTGCTTTGCAACAGTTAGAG
ATTGATGTTGTCTATTATGGTGTTATTCCAACGCCTGCGTTAGCTTACGTGGCTCAAGAATATGTTATTCCAGCCATTAT
GGTTACCGGCAGCCATATTCCTTTTGATCGTAACGGGCTTAAATTTTACCGTCCTGATGGCGAAATCAGTAAAGTTGACG
AGCAAGCTATTCTGACTGAGCAAGTTGAGTTTGCAGCGCTTGCTGAGCTACCTGAATTAGTTATTAATCATCGCGCTGCT
GAAGAATACATTACCCGTTATACCTCTTTATTTACTAGCCCTTGGCTTACTGGCAAACGCATTGGTATTTATGAGCATTC
CAGTGCCGGTCGCGATCTTTACTACCGTATTTTTGAAAAACTGGGTGCGGAAGTGATTGCGCTAGAGCGTAGTGATAAGT
TTGTGCCTATTGATACTGAAGCTGTTTCTGTTGAAGATACATTAAAAGCAATTAACTGGTCTAAAGAATATAATTTAGAT
GTTGTTTTTTCAACCGATGGTGATGGCGACCGCCCATTAGTATCCGATGAAAATGGGAACTGGCTGCGTGGGGATATTCT
CGGTTTATTAAGTGCTGAAGCATTAAATATTGAGGCGTTAGCTGTCCCTGTCAGCTGTAATACAGCGATTGAAAGCTGTA
ATAAATTTAAACATGTAGAGCGAACCAAAATTGGATCTCCTTATGTGATTGCTGAGTTTGCTAACTTAGCGGCTAAATTT
AATGCAGTCGCAGGCTTTGAAGCCAATGGAGGTTTTCTGCTGGGCAGTGATGTGCAATTAAACGGTCAAATATTAAAAGC
ATTGCCGACGCGTGATGCTGTTCTGCCTGCAATTATGTTGCTAGCTGCAGCAGGCGAAGGAGTTATTTCAACATTAGTAA
ATGCATTACCGCAACGTTTTACCTACAGTGATCGCATTCAAAATTTTGCGACTGAAAAAAGTCAGACGATTATTGCACAA
GGCAAAAATGATCCGGCAAATTTATTAATGCAGTTGGGTTTTGATAATGTTGAAATTAAAAATGTTGATGAAACAGATGG
TTTAAGAATAACATTAACAGACGAGCGAATTATTCATCTGCGACCGTCAGGTAATGCCCCGGAACTGCGTTGTTATGCAG
AAGCTGATAGTTACTCAAAAGCGCATGAGTTAGTAAATAACTCTTTAATAAACATACAAAAAATTTAA

Upstream 100 bases:

>100_bases
GGAAAGCTTAACAAGTATTGTCATTTTTCTCCGTGTGCGAAGCCTCTGTGTTCTCGGTGGTGAATTGTTTTTACCAGACC
CTGAATAATTTGGAAATATT

Downstream 100 bases:

>100_bases
ACTCACAAAGAGAAAGAAATGAGGTCTTTACATGTTTAAAGGTGTTGTTGTGGTTTCCTCCTTCTTTGCTCATTCATCTC
TCTTTGTAAATAATGTTTTA

Product: phosphoglucomutase/phosphomannomutase alpha/beta/subunit

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNKKLISATVIAESGIAFGTSGARGLVTQLTPDVCAAFAVAFIAGMTRNFNFKQVAIAIDNRPSSYAMAKACAAALQQLE
IDVVYYGVIPTPALAYVAQEYVIPAIMVTGSHIPFDRNGLKFYRPDGEISKVDEQAILTEQVEFAALAELPELVINHRAA
EEYITRYTSLFTSPWLTGKRIGIYEHSSAGRDLYYRIFEKLGAEVIALERSDKFVPIDTEAVSVEDTLKAINWSKEYNLD
VVFSTDGDGDRPLVSDENGNWLRGDILGLLSAEALNIEALAVPVSCNTAIESCNKFKHVERTKIGSPYVIAEFANLAAKF
NAVAGFEANGGFLLGSDVQLNGQILKALPTRDAVLPAIMLLAAAGEGVISTLVNALPQRFTYSDRIQNFATEKSQTIIAQ
GKNDPANLLMQLGFDNVEIKNVDETDGLRITLTDERIIHLRPSGNAPELRCYAEADSYSKAHELVNNSLINIQKI

Sequences:

>Translated_475_residues
MNKKLISATVIAESGIAFGTSGARGLVTQLTPDVCAAFAVAFIAGMTRNFNFKQVAIAIDNRPSSYAMAKACAAALQQLE
IDVVYYGVIPTPALAYVAQEYVIPAIMVTGSHIPFDRNGLKFYRPDGEISKVDEQAILTEQVEFAALAELPELVINHRAA
EEYITRYTSLFTSPWLTGKRIGIYEHSSAGRDLYYRIFEKLGAEVIALERSDKFVPIDTEAVSVEDTLKAINWSKEYNLD
VVFSTDGDGDRPLVSDENGNWLRGDILGLLSAEALNIEALAVPVSCNTAIESCNKFKHVERTKIGSPYVIAEFANLAAKF
NAVAGFEANGGFLLGSDVQLNGQILKALPTRDAVLPAIMLLAAAGEGVISTLVNALPQRFTYSDRIQNFATEKSQTIIAQ
GKNDPANLLMQLGFDNVEIKNVDETDGLRITLTDERIIHLRPSGNAPELRCYAEADSYSKAHELVNNSLINIQKI
>Mature_475_residues
MNKKLISATVIAESGIAFGTSGARGLVTQLTPDVCAAFAVAFIAGMTRNFNFKQVAIAIDNRPSSYAMAKACAAALQQLE
IDVVYYGVIPTPALAYVAQEYVIPAIMVTGSHIPFDRNGLKFYRPDGEISKVDEQAILTEQVEFAALAELPELVINHRAA
EEYITRYTSLFTSPWLTGKRIGIYEHSSAGRDLYYRIFEKLGAEVIALERSDKFVPIDTEAVSVEDTLKAINWSKEYNLD
VVFSTDGDGDRPLVSDENGNWLRGDILGLLSAEALNIEALAVPVSCNTAIESCNKFKHVERTKIGSPYVIAEFANLAAKF
NAVAGFEANGGFLLGSDVQLNGQILKALPTRDAVLPAIMLLAAAGEGVISTLVNALPQRFTYSDRIQNFATEKSQTIIAQ
GKNDPANLLMQLGFDNVEIKNVDETDGLRITLTDERIIHLRPSGNAPELRCYAEADSYSKAHELVNNSLINIQKI

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group C2 O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1789566, Length=362, Percent_Identity=27.6243093922652, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 51791; Mature: 51791

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLISATVIAESGIAFGTSGARGLVTQLTPDVCAAFAVAFIAGMTRNFNFKQVAIAID
CCCCEEEEEEEECCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
NRPSSYAMAKACAAALQQLEIDVVYYGVIPTPALAYVAQEYVIPAIMVTGSHIPFDRNGL
CCCCHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCC
KFYRPDGEISKVDEQAILTEQVEFAALAELPELVINHRAAEEYITRYTSLFTSPWLTGKR
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IGIYEHSSAGRDLYYRIFEKLGAEVIALERSDKFVPIDTEAVSVEDTLKAINWSKEYNLD
EEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCEECHHHHHHHHCCCCCCCEE
VVFSTDGDGDRPLVSDENGNWLRGDILGLLSAEALNIEALAVPVSCNTAIESCNKFKHVE
EEEECCCCCCCCEEECCCCCEEHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHHHHH
RTKIGSPYVIAEFANLAAKFNAVAGFEANGGFLLGSDVQLNGQILKALPTRDAVLPAIML
HHCCCCCEEHHHHHHHHHHHHHEECEECCCCEEEECCEEECCHHEEECCCCHHHHHHHHH
LAAAGEGVISTLVNALPQRFTYSDRIQNFATEKSQTIIAQGKNDPANLLMQLGFDNVEIK
HHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEEE
NVDETDGLRITLTDERIIHLRPSGNAPELRCYAEADSYSKAHELVNNSLINIQKI
CCCCCCCEEEEEECCEEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MNKKLISATVIAESGIAFGTSGARGLVTQLTPDVCAAFAVAFIAGMTRNFNFKQVAIAID
CCCCEEEEEEEECCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
NRPSSYAMAKACAAALQQLEIDVVYYGVIPTPALAYVAQEYVIPAIMVTGSHIPFDRNGL
CCCCHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCC
KFYRPDGEISKVDEQAILTEQVEFAALAELPELVINHRAAEEYITRYTSLFTSPWLTGKR
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IGIYEHSSAGRDLYYRIFEKLGAEVIALERSDKFVPIDTEAVSVEDTLKAINWSKEYNLD
EEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCEECHHHHHHHHCCCCCCCEE
VVFSTDGDGDRPLVSDENGNWLRGDILGLLSAEALNIEALAVPVSCNTAIESCNKFKHVE
EEEECCCCCCCCEEECCCCCEEHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHHHHH
RTKIGSPYVIAEFANLAAKFNAVAGFEANGGFLLGSDVQLNGQILKALPTRDAVLPAIML
HHCCCCCEEHHHHHHHHHHHHHEECEECCCCEEEECCEEECCHHEEECCCCHHHHHHHHH
LAAAGEGVISTLVNALPQRFTYSDRIQNFATEKSQTIIAQGKNDPANLLMQLGFDNVEIK
HHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEEE
NVDETDGLRITLTDERIIHLRPSGNAPELRCYAEADSYSKAHELVNNSLINIQKI
CCCCCCCEEEEEECCEEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1379320 [H]