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The map label for this gene is mdeA [H]

Identifier: 119944528

GI number: 119944528

Start: 951460

End: 952689

Strand: Direct

Name: mdeA [H]

Synonym: Ping_0757

Alternate gene names: 119944528

Gene position: 951460-952689 (Clockwise)

Preceding gene: 119944527

Following gene: 124021675

Centisome position: 20.87

GC content: 43.41

Gene sequence:

>1230_bases
ATGTCAGAACAAGGATTTACAACAAAAATAGTGCATTCAGACCATCTTCTGGAGTTAGAATATGGTGCAATACATGCTCC
CATCCATAATTCTGTCCCCTACGGTTTCCAAGACGTGCAGGGTTTAATTGATGTTTTCCAAGGTACAAAGCCAGGTCATG
CTTATGCGCGCTCATCCACACCCACTATTGATGCATTGCAGCATAAAATAAAACAGATGGACAATGGCATCGCGACGCTG
GTTTTTTCATGCGGCATGTCCGCAATAGTCACCACGTTTTTAACCCTGTTAAAAACCGGTGATCACCTTATCTGCAGCCA
TTATCTTTTTGGCAATACCAGAAGCATGATCGGCACACTTCAAGGTTACGGTATTGAAGTTACTTTTGTAGATTCAACTG
ATATTGAACAAGTAAAAGCCGCACAGCAACCCAATACCCGGATGCTTTTTGTCGAAACCATCGCTAATCCCGCCACTCAA
ATTAGCGCCTTAGATGAAATTGGTGATTTTTGTGAAGCGCAGGGTTTAGTCTATATTGTTGATAATACATTGACCTCCAG
TTACCTATTTGATGCAATAAAAGTCAAAGCCAGCCTGATTATCACATCTCTTTCTAAATACGTTGCCGGACATGGTAATG
CGTTGGGTGGCTCAGTCACCGATACGGGATTATTTGATTGGCAAACTTACCCAAATATCTTCGAAGCTTACCGCACGGGC
GACAGTAAAATGTGGGGCATAGGCCAAATAAAGAAAAAAGGCTTAAGGGATATGGGAGCTTGTATCAGTTCACAAACCGC
GCACCTTATTTCAGTCGGCAGCGAAACAATGGAACTGCGTATGCAGCGCCAGTGTAAAAACGCAGGTATATTAGCTGAAT
TCTTAGCGCAGCACCCGAAAGTTGCCAAAGTCTATTATCCGGGACTAAAAGATCACCCTCAACACCAAAGAGCGAAACGT
TTATTTAAACACTTTGGCGCCATGTTAAGTTTTGATCTTATTGAAGGCGAAGACTGTTGTGAGTTATTAAATAAACTAAA
ATTGGTGATCAGCGCAACCCACCTCGGTGATAACCGTACCCTCGCATTACCCGTTGCACCAACCATCTACCATGAGATGG
GACTTGAGACCCGCCAGAAAATGGGCATCAGTGAGACTATGGTACGTTGTTCAATCGGTATCGAAAATACCGCCGACTTA
ATAGCTGATTTCGAGCAGGCATTGGCTTAA

Upstream 100 bases:

>100_bases
AAAAAAGACCAAAATGCTATATAGGTGGATTTTAACTTAGTTGCCTATCTCTGTATAATACCCCCATCGCTGAATTTAAT
TAGATTACCAAGGAATTTTG

Downstream 100 bases:

>100_bases
GCGTTATCTGAAATTGCTGCCTGATTTAAAAAGCACAACTTAAGGTGGGCCGGGTTAGCTAATACGCCCGTCATTTCCGA
GTTGTTCTGTCGGGAAGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSEQGFTTKIVHSDHLLELEYGAIHAPIHNSVPYGFQDVQGLIDVFQGTKPGHAYARSSTPTIDALQHKIKQMDNGIATL
VFSCGMSAIVTTFLTLLKTGDHLICSHYLFGNTRSMIGTLQGYGIEVTFVDSTDIEQVKAAQQPNTRMLFVETIANPATQ
ISALDEIGDFCEAQGLVYIVDNTLTSSYLFDAIKVKASLIITSLSKYVAGHGNALGGSVTDTGLFDWQTYPNIFEAYRTG
DSKMWGIGQIKKKGLRDMGACISSQTAHLISVGSETMELRMQRQCKNAGILAEFLAQHPKVAKVYYPGLKDHPQHQRAKR
LFKHFGAMLSFDLIEGEDCCELLNKLKLVISATHLGDNRTLALPVAPTIYHEMGLETRQKMGISETMVRCSIGIENTADL
IADFEQALA

Sequences:

>Translated_409_residues
MSEQGFTTKIVHSDHLLELEYGAIHAPIHNSVPYGFQDVQGLIDVFQGTKPGHAYARSSTPTIDALQHKIKQMDNGIATL
VFSCGMSAIVTTFLTLLKTGDHLICSHYLFGNTRSMIGTLQGYGIEVTFVDSTDIEQVKAAQQPNTRMLFVETIANPATQ
ISALDEIGDFCEAQGLVYIVDNTLTSSYLFDAIKVKASLIITSLSKYVAGHGNALGGSVTDTGLFDWQTYPNIFEAYRTG
DSKMWGIGQIKKKGLRDMGACISSQTAHLISVGSETMELRMQRQCKNAGILAEFLAQHPKVAKVYYPGLKDHPQHQRAKR
LFKHFGAMLSFDLIEGEDCCELLNKLKLVISATHLGDNRTLALPVAPTIYHEMGLETRQKMGISETMVRCSIGIENTADL
IADFEQALA
>Mature_408_residues
SEQGFTTKIVHSDHLLELEYGAIHAPIHNSVPYGFQDVQGLIDVFQGTKPGHAYARSSTPTIDALQHKIKQMDNGIATLV
FSCGMSAIVTTFLTLLKTGDHLICSHYLFGNTRSMIGTLQGYGIEVTFVDSTDIEQVKAAQQPNTRMLFVETIANPATQI
SALDEIGDFCEAQGLVYIVDNTLTSSYLFDAIKVKASLIITSLSKYVAGHGNALGGSVTDTGLFDWQTYPNIFEAYRTGD
SKMWGIGQIKKKGLRDMGACISSQTAHLISVGSETMELRMQRQCKNAGILAEFLAQHPKVAKVYYPGLKDHPQHQRAKRL
FKHFGAMLSFDLIEGEDCCELLNKLKLVISATHLGDNRTLALPVAPTIYHEMGLETRQKMGISETMVRCSIGIENTADLI
ADFEQALA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=364, Percent_Identity=29.9450549450549, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI299473759, Length=363, Percent_Identity=26.4462809917355, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI25453487, Length=363, Percent_Identity=26.9972451790634, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1790375, Length=391, Percent_Identity=29.923273657289, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789383, Length=416, Percent_Identity=23.0769230769231, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71980708, Length=399, Percent_Identity=30.8270676691729, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=368, Percent_Identity=30.9782608695652, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17560056, Length=369, Percent_Identity=30.3523035230352, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6323333, Length=408, Percent_Identity=29.4117647058824, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319307, Length=414, Percent_Identity=26.3285024154589, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=288, Percent_Identity=23.2638888888889, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321254, Length=409, Percent_Identity=25.4278728606357, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322970, Length=310, Percent_Identity=24.1935483870968, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922554, Length=410, Percent_Identity=29.7560975609756, Blast_Score=171, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 44915; Mature: 44784

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQGFTTKIVHSDHLLELEYGAIHAPIHNSVPYGFQDVQGLIDVFQGTKPGHAYARSST
CCCCCCEEEEEECCCEEEEECCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PTIDALQHKIKQMDNGIATLVFSCGMSAIVTTFLTLLKTGDHLICSHYLFGNTRSMIGTL
CHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHH
QGYGIEVTFVDSTDIEQVKAAQQPNTRMLFVETIANPATQISALDEIGDFCEAQGLVYIV
CCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHCCEEEEE
DNTLTSSYLFDAIKVKASLIITSLSKYVAGHGNALGGSVTDTGLFDWQTYPNIFEAYRTG
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
DSKMWGIGQIKKKGLRDMGACISSQTAHLISVGSETMELRMQRQCKNAGILAEFLAQHPK
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCC
VAKVYYPGLKDHPQHQRAKRLFKHFGAMLSFDLIEGEDCCELLNKLKLVISATHLGDNRT
EEEEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHEEEECCCCCE
LALPVAPTIYHEMGLETRQKMGISETMVRCSIGIENTADLIADFEQALA
EEEECCHHHHHHHCCHHHHHCCHHHHHHHEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SEQGFTTKIVHSDHLLELEYGAIHAPIHNSVPYGFQDVQGLIDVFQGTKPGHAYARSST
CCCCCEEEEEECCCEEEEECCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PTIDALQHKIKQMDNGIATLVFSCGMSAIVTTFLTLLKTGDHLICSHYLFGNTRSMIGTL
CHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHH
QGYGIEVTFVDSTDIEQVKAAQQPNTRMLFVETIANPATQISALDEIGDFCEAQGLVYIV
CCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHCCEEEEE
DNTLTSSYLFDAIKVKASLIITSLSKYVAGHGNALGGSVTDTGLFDWQTYPNIFEAYRTG
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
DSKMWGIGQIKKKGLRDMGACISSQTAHLISVGSETMELRMQRQCKNAGILAEFLAQHPK
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCC
VAKVYYPGLKDHPQHQRAKRLFKHFGAMLSFDLIEGEDCCELLNKLKLVISATHLGDNRT
EEEEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHEEEECCCCCE
LALPVAPTIYHEMGLETRQKMGISETMVRCSIGIENTADLIADFEQALA
EEEECCHHHHHHHCCHHHHHCCHHHHHHHEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]