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The map label for this gene is tolB [H]

Identifier: 119944498

GI number: 119944498

Start: 919905

End: 921218

Strand: Direct

Name: tolB [H]

Synonym: Ping_0727

Alternate gene names: 119944498

Gene position: 919905-921218 (Clockwise)

Preceding gene: 119944497

Following gene: 119944499

Centisome position: 20.18

GC content: 41.55

Gene sequence:

>1314_bases
ATGCTGTTACGTTTATTTTTATCAATTTCAATGATTATATTGTCGTCTCAGGCTTCTGCCCGCTTAGAAATTTTAATTAC
CGAAGGGGTTAATACTGCTAGGCCGATCGCTGTTATTCCATTTAAGTGGTTAGGTGATGGCGAAAAACCGGGAAATTTTT
CAGAGATTATCGCTGCAGATTTACAACGTAGTGGTCAATTTAGTCCCCTTCCTATTGAGCAAATGCCTAATACTCCGTCA
AAGAGTCTCGGCCTTGATTATGCCCTGTGGCATAAAATGGGGATTGAAGCTTTATTGATGGGGGAAGTCTCACCTTCACC
GGAAGCCGGGAAATATATTGTTACCTATGAATTGGTAGACGTTGTGGCGGGAAATGCAAATATTGCTGACTACAGTCCTA
TTTTAGAAAGTCGCCGTGGCACCGTAAATAAAACTCAATTACGTCGCTTTGCACATCGTATTAGTGATGTTGCCTATGAA
AAATTAACGGGTGAAAAAGGCGTATTTTTAACCCGGATTGCCTATATTGCAGTGGATCGCAAAAGTGACTATCCCTACCA
ATTACGTATTTCTGATTATGACGGTTATAACGAAAAATTAATACTGCGTTCAAAACAGCCTATTATGTCACCAACATGGT
CTCCGGATGGAAGAAAATTGGCCTATGTTAGCTTTGAGCACCGCCGTTCAGAAGTCTATATACAAGATTTATATAGCCAA
AAACGTGAGTTAATGACCTCTTTTCCAAAAATAAATGGCTCACCCAGTTGGTCCCCAGACGGTAAATCTTTGGCAATAGT
ATTATCAAAAGACGGGCAGCCCGAGATCTACACCTTAGAAATACAAAGCAAAGCGTTACGTAGAATCACTAATAATCGCG
CTATAGATACCGAACCTAATTGGTCGCCAGACGGCAATAGTTTAACTTTTACTTCTACTCGCGGTGGAGGGCCTCAAATT
TATCAAGTTGATCTTCAATCGAAAGTGACAAAACGGCTTACCTGGGAAGGTGATCTTAATTTAGGTGGTGCAATTACCCC
CGATGGTGCTAATCTAGTGCTCGTTAGCCGCCTTGATGGTAATTATAAAATAGCCGCACAAGATCTGGCCTCTGGCGAAC
TAAGAACCTTGACCAAAACACATTTAGACGAGTCACCAAGCGTTGCGCCGAATGGCAGTATGATAATTTACAGTACTGTT
TATAAAGGTAGTCAAGGTCTGGCGTTAGTCTCTATGGACGGTCGTTTTAAAGCGAATATTCCTGCACAAAGTGGCGAGGT
TAAATCGCCTGCGTGGTCTCCATTTTTAAAGTAA

Upstream 100 bases:

>100_bases
AAGCAAACACATTACCTATACCTAAAGATCCGGATATTTCAGCAAAATTCCGTGATTTTGATCTTAATTTAAAACCTGAA
TTTTAAGGAAACCAAGCCAT

Downstream 100 bases:

>100_bases
GTTAATTAACAACTAAAAAGGGAAAAATATGAAACTAAGTATGATTTCAAAAAGTTTATTAATTGCACTGCCACTGTTAG
CTTTGAGTGCTTGTAGTTCA

Product: TolB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MLLRLFLSISMIILSSQASARLEILITEGVNTARPIAVIPFKWLGDGEKPGNFSEIIAADLQRSGQFSPLPIEQMPNTPS
KSLGLDYALWHKMGIEALLMGEVSPSPEAGKYIVTYELVDVVAGNANIADYSPILESRRGTVNKTQLRRFAHRISDVAYE
KLTGEKGVFLTRIAYIAVDRKSDYPYQLRISDYDGYNEKLILRSKQPIMSPTWSPDGRKLAYVSFEHRRSEVYIQDLYSQ
KRELMTSFPKINGSPSWSPDGKSLAIVLSKDGQPEIYTLEIQSKALRRITNNRAIDTEPNWSPDGNSLTFTSTRGGGPQI
YQVDLQSKVTKRLTWEGDLNLGGAITPDGANLVLVSRLDGNYKIAAQDLASGELRTLTKTHLDESPSVAPNGSMIIYSTV
YKGSQGLALVSMDGRFKANIPAQSGEVKSPAWSPFLK

Sequences:

>Translated_437_residues
MLLRLFLSISMIILSSQASARLEILITEGVNTARPIAVIPFKWLGDGEKPGNFSEIIAADLQRSGQFSPLPIEQMPNTPS
KSLGLDYALWHKMGIEALLMGEVSPSPEAGKYIVTYELVDVVAGNANIADYSPILESRRGTVNKTQLRRFAHRISDVAYE
KLTGEKGVFLTRIAYIAVDRKSDYPYQLRISDYDGYNEKLILRSKQPIMSPTWSPDGRKLAYVSFEHRRSEVYIQDLYSQ
KRELMTSFPKINGSPSWSPDGKSLAIVLSKDGQPEIYTLEIQSKALRRITNNRAIDTEPNWSPDGNSLTFTSTRGGGPQI
YQVDLQSKVTKRLTWEGDLNLGGAITPDGANLVLVSRLDGNYKIAAQDLASGELRTLTKTHLDESPSVAPNGSMIIYSTV
YKGSQGLALVSMDGRFKANIPAQSGEVKSPAWSPFLK
>Mature_437_residues
MLLRLFLSISMIILSSQASARLEILITEGVNTARPIAVIPFKWLGDGEKPGNFSEIIAADLQRSGQFSPLPIEQMPNTPS
KSLGLDYALWHKMGIEALLMGEVSPSPEAGKYIVTYELVDVVAGNANIADYSPILESRRGTVNKTQLRRFAHRISDVAYE
KLTGEKGVFLTRIAYIAVDRKSDYPYQLRISDYDGYNEKLILRSKQPIMSPTWSPDGRKLAYVSFEHRRSEVYIQDLYSQ
KRELMTSFPKINGSPSWSPDGKSLAIVLSKDGQPEIYTLEIQSKALRRITNNRAIDTEPNWSPDGNSLTFTSTRGGGPQI
YQVDLQSKVTKRLTWEGDLNLGGAITPDGANLVLVSRLDGNYKIAAQDLASGELRTLTKTHLDESPSVAPNGSMIIYSTV
YKGSQGLALVSMDGRFKANIPAQSGEVKSPAWSPFLK

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=426, Percent_Identity=45.3051643192488, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 48340; Mature: 48340

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRLFLSISMIILSSQASARLEILITEGVNTARPIAVIPFKWLGDGEKPGNFSEIIAAD
CCEEHHHHHHHHEEECCCCCEEEEEEECCCCCCCCEEEEEEHCCCCCCCCCCHHHHHHHH
LQRSGQFSPLPIEQMPNTPSKSLGLDYALWHKMGIEALLMGEVSPSPEAGKYIVTYELVD
HHHCCCCCCCCHHHCCCCCCHHCCCCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEEE
VVAGNANIADYSPILESRRGTVNKTQLRRFAHRISDVAYEKLTGEKGVFLTRIAYIAVDR
HHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEC
KSDYPYQLRISDYDGYNEKLILRSKQPIMSPTWSPDGRKLAYVSFEHRRSEVYIQDLYSQ
CCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHH
KRELMTSFPKINGSPSWSPDGKSLAIVLSKDGQPEIYTLEIQSKALRRITNNRAIDTEPN
HHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHCCCCCCCCCC
WSPDGNSLTFTSTRGGGPQIYQVDLQSKVTKRLTWEGDLNLGGAITPDGANLVLVSRLDG
CCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHEEECCCCCCCCEECCCCCCEEEEEECCC
NYKIAAQDLASGELRTLTKTHLDESPSVAPNGSMIIYSTVYKGSQGLALVSMDGRFKANI
CEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCEEEECC
PAQSGEVKSPAWSPFLK
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLLRLFLSISMIILSSQASARLEILITEGVNTARPIAVIPFKWLGDGEKPGNFSEIIAAD
CCEEHHHHHHHHEEECCCCCEEEEEEECCCCCCCCEEEEEEHCCCCCCCCCCHHHHHHHH
LQRSGQFSPLPIEQMPNTPSKSLGLDYALWHKMGIEALLMGEVSPSPEAGKYIVTYELVD
HHHCCCCCCCCHHHCCCCCCHHCCCCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEEE
VVAGNANIADYSPILESRRGTVNKTQLRRFAHRISDVAYEKLTGEKGVFLTRIAYIAVDR
HHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEC
KSDYPYQLRISDYDGYNEKLILRSKQPIMSPTWSPDGRKLAYVSFEHRRSEVYIQDLYSQ
CCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHH
KRELMTSFPKINGSPSWSPDGKSLAIVLSKDGQPEIYTLEIQSKALRRITNNRAIDTEPN
HHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHCCCCCCCCCC
WSPDGNSLTFTSTRGGGPQIYQVDLQSKVTKRLTWEGDLNLGGAITPDGANLVLVSRLDG
CCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHEEECCCCCCCCEECCCCCCEEEEEECCC
NYKIAAQDLASGELRTLTKTHLDESPSVAPNGSMIIYSTVYKGSQGLALVSMDGRFKANI
CEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCEEEECC
PAQSGEVKSPAWSPFLK
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA