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The map label for this gene is rpoS [H]

Identifier: 119944449

GI number: 119944449

Start: 864787

End: 865755

Strand: Direct

Name: rpoS [H]

Synonym: Ping_0677

Alternate gene names: 119944449

Gene position: 864787-865755 (Clockwise)

Preceding gene: 119944448

Following gene: 119944451

Centisome position: 18.97

GC content: 38.7

Gene sequence:

>969_bases
ATGATTAAGGATTTAAAATTAGAGAGCAAAAAAAGTCTCCCTTTTGATCTGGATGAAAATGCAGATACAGATACAGATAC
AATTGATGATGAAGATTTTAGTGCCGCTAAGATAGCTGTTGCTAATGAAGTAAAAAGCATGGATGCAACTCAACTTTATT
TAGGTGAAATTGGTTTCTCGCCACTATTAACAGCAGAAGAAGAAGTTTATTACGCCCGGCGTTTACTGCGCGGTGATCAA
GTATCACGTAAACGCATGATAGAAAGTAATTTACGCCTGGTAGTGAAAATTTCCCGTCGTTATAATAATCGTGGATTATC
ATTATTAGATTTAGTTGAAGAGGGTAACCTAGGGCTTATTCGGGCTGTTGAAAAATTTGATCCGGAAAAAGGCTTTCGCT
TTTCAACCTATGCAACCTGGTGGATACGTCAAACGATTGAACGCGCGATAATGAATCAAACCCGTACTATTCGTTTGCCT
ATCCACATAGTAAAAAAATTAAATGTGTATTTACGAACTGCCCGTGAATTAACACACACGCTTACTCACGAACCAACGGC
TGAAGAGATCGCCGAAGTTTTGGGAGTCTCCGCGAAAGAAGTCTCTAAGATTTTAAAACTTAATGAGCGTATTAGTTCCG
TTGATATCCCCATAGGAAATAATTCTGATAAAGAATTATTGGATATTATTCCAGATCGAAAAGAGGCTTCACCGGAAAAT
GAACTGCAAGAGGCTGATATGAAACTGCGTATTGTTAAGTGGTTAGAAGACCTTAATCCTAAACAGCGTGAAGTATTAGC
AAGACGATTTGGTTTGTTAGGTTATGAAGCTGCAACACTTGAAAATGTGGGGAAAGAGATCGGTCTGACACGCGAACGTG
TGCGGCAGATACAAGTTGAAGCGCTAAAATATTTACGTGATGTACTCTCTGAGCAGGGTTTATCAATCGAATCATTGTTT
CAAGTTTAG

Upstream 100 bases:

>100_bases
GAATGGAATAGCTTTTTTATGGGCAGATATCTATAAATCTTAACATAAAATAACAGGTTTTTTGTAATAGCAGGTTAAGC
TTTTTAAAGAGGACGTGGTT

Downstream 100 bases:

>100_bases
CTATCAAAAGCGCATTAAAAAAAGCATCTTTTGATGCAATGCCGATAGGTTAAGGACTTTTTAAGAAAACTGGAACGATT
TTACAGAGAGATAATAATCC

Product: RNA polymerase sigma-38 factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MIKDLKLESKKSLPFDLDENADTDTDTIDDEDFSAAKIAVANEVKSMDATQLYLGEIGFSPLLTAEEEVYYARRLLRGDQ
VSRKRMIESNLRLVVKISRRYNNRGLSLLDLVEEGNLGLIRAVEKFDPEKGFRFSTYATWWIRQTIERAIMNQTRTIRLP
IHIVKKLNVYLRTARELTHTLTHEPTAEEIAEVLGVSAKEVSKILKLNERISSVDIPIGNNSDKELLDIIPDRKEASPEN
ELQEADMKLRIVKWLEDLNPKQREVLARRFGLLGYEAATLENVGKEIGLTRERVRQIQVEALKYLRDVLSEQGLSIESLF
QV

Sequences:

>Translated_322_residues
MIKDLKLESKKSLPFDLDENADTDTDTIDDEDFSAAKIAVANEVKSMDATQLYLGEIGFSPLLTAEEEVYYARRLLRGDQ
VSRKRMIESNLRLVVKISRRYNNRGLSLLDLVEEGNLGLIRAVEKFDPEKGFRFSTYATWWIRQTIERAIMNQTRTIRLP
IHIVKKLNVYLRTARELTHTLTHEPTAEEIAEVLGVSAKEVSKILKLNERISSVDIPIGNNSDKELLDIIPDRKEASPEN
ELQEADMKLRIVKWLEDLNPKQREVLARRFGLLGYEAATLENVGKEIGLTRERVRQIQVEALKYLRDVLSEQGLSIESLF
QV
>Mature_322_residues
MIKDLKLESKKSLPFDLDENADTDTDTIDDEDFSAAKIAVANEVKSMDATQLYLGEIGFSPLLTAEEEVYYARRLLRGDQ
VSRKRMIESNLRLVVKISRRYNNRGLSLLDLVEEGNLGLIRAVEKFDPEKGFRFSTYATWWIRQTIERAIMNQTRTIRLP
IHIVKKLNVYLRTARELTHTLTHEPTAEEIAEVLGVSAKEVSKILKLNERISSVDIPIGNNSDKELLDIIPDRKEASPEN
ELQEADMKLRIVKWLEDLNPKQREVLARRFGLLGYEAATLENVGKEIGLTRERVRQIQVEALKYLRDVLSEQGLSIESLF
QV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=303, Percent_Identity=72.2772277227723, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1789448, Length=247, Percent_Identity=41.2955465587045, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1789871, Length=275, Percent_Identity=28.7272727272727, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36953; Mature: 36953

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDLKLESKKSLPFDLDENADTDTDTIDDEDFSAAKIAVANEVKSMDATQLYLGEIGFS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCC
PLLTAEEEVYYARRLLRGDQVSRKRMIESNLRLVVKISRRYNNRGLSLLDLVEEGNLGLI
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEEHHHCCCCCHHHHHHHCCCCHHH
RAVEKFDPEKGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKKLNVYLRTARELTHT
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LTHEPTAEEIAEVLGVSAKEVSKILKLNERISSVDIPIGNNSDKELLDIIPDRKEASPEN
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCHH
ELQEADMKLRIVKWLEDLNPKQREVLARRFGLLGYEAATLENVGKEIGLTRERVRQIQVE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHH
ALKYLRDVLSEQGLSIESLFQV
HHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MIKDLKLESKKSLPFDLDENADTDTDTIDDEDFSAAKIAVANEVKSMDATQLYLGEIGFS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCC
PLLTAEEEVYYARRLLRGDQVSRKRMIESNLRLVVKISRRYNNRGLSLLDLVEEGNLGLI
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEEHHHCCCCCHHHHHHHCCCCHHH
RAVEKFDPEKGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKKLNVYLRTARELTHT
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LTHEPTAEEIAEVLGVSAKEVSKILKLNERISSVDIPIGNNSDKELLDIIPDRKEASPEN
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCHH
ELQEADMKLRIVKWLEDLNPKQREVLARRFGLLGYEAATLENVGKEIGLTRERVRQIQVE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHH
ALKYLRDVLSEQGLSIESLFQV
HHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]