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The map label for this gene is proW [H]

Identifier: 119944387

GI number: 119944387

Start: 796626

End: 797696

Strand: Reverse

Name: proW [H]

Synonym: Ping_0615

Alternate gene names: 119944387

Gene position: 797696-796626 (Counterclockwise)

Preceding gene: 119944388

Following gene: 119944386

Centisome position: 17.49

GC content: 48.09

Gene sequence:

>1071_bases
ATGGCAACAGAAACAACAACTCAAGCCGCTGACCCTTGGGCCAGTGCAGCAGCGCCGGCCGATCCATGGACGACGGCCTC
CTCCGCAACATCAACCGCGAATGATTGGTTGAACGTTGCCACCGAAGCCGAGCCTTTTGATTGGTTGCATCCCTTCCAGG
AGGCTATTATGCCTTTTGATCAGTGGGTTGAGACCGGGCTTAACTGGTTAGTGGTTCACGGGCGTCCGGTATTTCAGGCA
ATCCGTGTCCCGATTGATTTGATTCTCACTTCATTTCAAAGTGCATTGGTATCAACGCCCGCTCCGATTATGTTACTTAT
TTTAGCCTTGATTGCCTGGCAACTAGCCGGACCCCGAATGGGAATTACCTCTTTATTCTCTTTGATTATCATTGGACTGG
TCGGCGCGTGGTCACAAGCGATGGTGACACTCGCACTGGTGATGACCTCAGTATTTTTCTGTTTTTTGATGGGCTTACCC
TTAGGGATCTGGTTGGCTCGAAGTCAATCAGCCGCGAAAATAATTCGACCTATGCTTGATGCGATGCAAACCACACCCGC
TTTTGTCTATTTAGTGCCTATTGTTATGCTCTTTGGTATCGGTAATGTTCCGGGTGTTGTGGTGACTATTATTTTCGCCT
TACCACCCATTGTCCGTCTGACTATTCTAGGTATTCAGCAAGTACCGGAAGAGCTGATCGAAGCAGGGCACGCTTTTGGT
GCCAGCCCGAAACAGATGCTTTATCGGATTCAACTCCCCTTAGCAATGCCCACTATTATGGCCGGAGTGAACCAAACTCT
CATGTTGTCGCTGTCCATGGTGGTGATAGCCTCTATGATTGCCGTCGGTGGTCTGGGACAAATGGTGTTACGCGGGATTG
GACGACTGGATATGGGTCTGGCGGCTGTCGGTGGTCTTGGGATTGTTATTCTTGCTATCTTGCTTGATCGTATTACGCAA
ACCATAGGTAAAAACAAACGCGATCAAAAAACGCGCTGGTATCAAACAGGCCCCGTTTCATTGATTTATTTACTGTTAAA
AAAACTTAACCACAATAAAACTGTAAACTAG

Upstream 100 bases:

>100_bases
CTGACGTGCGTTATGCCGTACCCGTTGTTGCTGAAGATGGCACCTACCACGGGGTGATCACTAAATCACGCTTATTAAAA
ATTTTAGATAGGGAATAATC

Downstream 100 bases:

>100_bases
GAGAATAAAATGAAGTATATACGAAAGACAATACTTACAACGGCAATCGCTGTTTCAGCAACCCTGTCATCCGCTGTCTG
GGCTGAAAACCTGCCAGGCG

Product: glycine betaine ABC transporter, binding protein inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MATETTTQAADPWASAAAPADPWTTASSATSTANDWLNVATEAEPFDWLHPFQEAIMPFDQWVETGLNWLVVHGRPVFQA
IRVPIDLILTSFQSALVSTPAPIMLLILALIAWQLAGPRMGITSLFSLIIIGLVGAWSQAMVTLALVMTSVFFCFLMGLP
LGIWLARSQSAAKIIRPMLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGIQQVPEELIEAGHAFG
ASPKQMLYRIQLPLAMPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGLGIVILAILLDRITQ
TIGKNKRDQKTRWYQTGPVSLIYLLLKKLNHNKTVN

Sequences:

>Translated_356_residues
MATETTTQAADPWASAAAPADPWTTASSATSTANDWLNVATEAEPFDWLHPFQEAIMPFDQWVETGLNWLVVHGRPVFQA
IRVPIDLILTSFQSALVSTPAPIMLLILALIAWQLAGPRMGITSLFSLIIIGLVGAWSQAMVTLALVMTSVFFCFLMGLP
LGIWLARSQSAAKIIRPMLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGIQQVPEELIEAGHAFG
ASPKQMLYRIQLPLAMPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGLGIVILAILLDRITQ
TIGKNKRDQKTRWYQTGPVSLIYLLLKKLNHNKTVN
>Mature_355_residues
ATETTTQAADPWASAAAPADPWTTASSATSTANDWLNVATEAEPFDWLHPFQEAIMPFDQWVETGLNWLVVHGRPVFQAI
RVPIDLILTSFQSALVSTPAPIMLLILALIAWQLAGPRMGITSLFSLIIIGLVGAWSQAMVTLALVMTSVFFCFLMGLPL
GIWLARSQSAAKIIRPMLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGIQQVPEELIEAGHAFGA
SPKQMLYRIQLPLAMPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGLGIVILAILLDRITQT
IGKNKRDQKTRWYQTGPVSLIYLLLKKLNHNKTVN

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=325, Percent_Identity=68.3076923076923, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1788451, Length=205, Percent_Identity=28.780487804878, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1786564, Length=154, Percent_Identity=30.5194805194805, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788449, Length=181, Percent_Identity=26.5193370165746, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 38490; Mature: 38358

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATETTTQAADPWASAAAPADPWTTASSATSTANDWLNVATEAEPFDWLHPFQEAIMPFD
CCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCHH
QWVETGLNWLVVHGRPVFQAIRVPIDLILTSFQSALVSTPAPIMLLILALIAWQLAGPRM
HHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
GITSLFSLIIIGLVGAWSQAMVTLALVMTSVFFCFLMGLPLGIWLARSQSAAKIIRPMLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGIQQVPEELIEAGHAFG
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASPKQMLYRIQLPLAMPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGL
CCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHH
AAVGGLGIVILAILLDRITQTIGKNKRDQKTRWYQTGPVSLIYLLLKKLNHNKTVN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ATETTTQAADPWASAAAPADPWTTASSATSTANDWLNVATEAEPFDWLHPFQEAIMPFD
CCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCHH
QWVETGLNWLVVHGRPVFQAIRVPIDLILTSFQSALVSTPAPIMLLILALIAWQLAGPRM
HHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
GITSLFSLIIIGLVGAWSQAMVTLALVMTSVFFCFLMGLPLGIWLARSQSAAKIIRPMLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGIQQVPEELIEAGHAFG
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASPKQMLYRIQLPLAMPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGL
CCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHH
AAVGGLGIVILAILLDRITQTIGKNKRDQKTRWYQTGPVSLIYLLLKKLNHNKTVN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]