Click here to switch to the map view.

The map label for this gene is mscL

Identifier: 119900111

GI number: 119900111

Start: 4182309

End: 4182734

Strand: Direct

Name: mscL

Synonym: azo3822

Alternate gene names: 119900111

Gene position: 4182309-4182734 (Clockwise)

Preceding gene: 119900106

Following gene: 119900114

Centisome position: 95.57

GC content: 63.15

Gene sequence:

>426_bases
ATGAGCTTCATCCAGGAATTCAAGGAATTCGCCATGCGCGGCAACGTCATCGACCTTGCCGTGGGCGTCATCATCGGTGG
CGCCTTCGGCAAGATCGTCGATTCGCTGGTGAAGGACGTGGTGATGCCGATCGTGGGCCGCCTGGTCGGCGGGGTGGACT
TCCGCCATCTCTACGTCAACCTCGGCAGTCAGCAGTACGAAACCCTGGAAGCCGCCGAAAAGGCCGGCGCGCCGCTGGTG
AAGTACGGCGCCTTCATCAACACCACGATCGACTTCCTGATCATCGCGCTGGCGATCTTCGTCGCGATCAAGGCGATCAA
CAAACTCAAGCGCAGCGAACCGCCCGCGCCGGCGCCCGAACCCGCGCCGGAACCGGAGGACATCAAGCTGCTGCGCGAGA
TCCGCGACGCGCTGAAGCAGCGCTGA

Upstream 100 bases:

>100_bases
GCAGCGCCGCGGCCATCCCGATACCATGCGGTCAGCACACAGGCCTAGGCAAACGTCCTGCCGCAAACCCGCCCATCCCC
GCAAAACCAAGGAGAAACCG

Downstream 100 bases:

>100_bases
CGCGCCGTGGCGGCCGCGCCTGCCTCAGGCGTGGTCGCCCACGTAGGGATTGCTGTCGCGCTCGTCGCCGAAGGTGGAGG
CCGGGCCGTGACCCGGGATG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MSFIQEFKEFAMRGNVIDLAVGVIIGGAFGKIVDSLVKDVVMPIVGRLVGGVDFRHLYVNLGSQQYETLEAAEKAGAPLV
KYGAFINTTIDFLIIALAIFVAIKAINKLKRSEPPAPAPEPAPEPEDIKLLREIRDALKQR

Sequences:

>Translated_141_residues
MSFIQEFKEFAMRGNVIDLAVGVIIGGAFGKIVDSLVKDVVMPIVGRLVGGVDFRHLYVNLGSQQYETLEAAEKAGAPLV
KYGAFINTTIDFLIIALAIFVAIKAINKLKRSEPPAPAPEPAPEPEDIKLLREIRDALKQR
>Mature_140_residues
SFIQEFKEFAMRGNVIDLAVGVIIGGAFGKIVDSLVKDVVMPIVGRLVGGVDFRHLYVNLGSQQYETLEAAEKAGAPLVK
YGAFINTTIDFLIIALAIFVAIKAINKLKRSEPPAPAPEPAPEPEDIKLLREIRDALKQR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=141, Percent_Identity=55.3191489361702, Blast_Score=141, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_AZOSB (A1KC82)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_935324.1
- ProteinModelPortal:   A1KC82
- SMR:   A1KC82
- STRING:   A1KC82
- GeneID:   4609347
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3822
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   A1KC82
- ProtClustDB:   PRK13952
- BioCyc:   ASP62928:AZO3822-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15404; Mature: 15273

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1dcfe834)-; HASH(0x1f9cee0c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIQEFKEFAMRGNVIDLAVGVIIGGAFGKIVDSLVKDVVMPIVGRLVGGVDFRHLYVN
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LGSQQYETLEAAEKAGAPLVKYGAFINTTIDFLIIALAIFVAIKAINKLKRSEPPAPAPE
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAPEPEDIKLLREIRDALKQR
CCCCCHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SFIQEFKEFAMRGNVIDLAVGVIIGGAFGKIVDSLVKDVVMPIVGRLVGGVDFRHLYVN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LGSQQYETLEAAEKAGAPLVKYGAFINTTIDFLIIALAIFVAIKAINKLKRSEPPAPAPE
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAPEPEDIKLLREIRDALKQR
CCCCCHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA