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The map label for this gene is ptsI [H]

Identifier: 119900071

GI number: 119900071

Start: 4143071

End: 4144801

Strand: Direct

Name: ptsI [H]

Synonym: azo3782

Alternate gene names: 119900071

Gene position: 4143071-4144801 (Clockwise)

Preceding gene: 119900070

Following gene: 119900072

Centisome position: 94.68

GC content: 68.11

Gene sequence:

>1731_bases
ATGCCGTTTACGCTGCACGGCCTGCCGGTGTCGCAGGGCATCGCGATCGGACATGTGCATCTGGTCTCGCATGCGCTGCT
CGAGGTCAACCACTACCACGTCGCGCAGCGCCATCTCGCCGATGAAACCGCACGCCTGGACGACGCGGTCGCGACCGTCC
AGGGCGAGCTGATCGGACTCAAGGCGGCCGCCACCGGCGGCCAGGCGCACAGCGAGGTCGGCGCCTTCGTCGACCTGCAG
CTGATGATGCTGGCCGACCCGATGCTGATCGACGCCGCGCGCGCGCTGATCGAATCGCGCGGCTGCAACGCCGAGTGGGC
GCTGGTGCAGCAGATGGAACTGGTCGTGGCACAGTTCCGCCAGATCGAAGACCCCTATCTGCGCGAACGTCAGGCCGACG
TCGTGCAGGTGGTCGAACGCCTCGTCAAGGTACTGCTCGGCCATCCGGGTCACTTGCCGCCTCGCCGGCGCGACGGACTG
GGCACCATCGTCGTCGCGCACGACCTGTCGCCCGCCGACACCATTGGCTTTCGCGACCACAACATCGCCGGCTTCGTCAC
CGACGTCGGCGGCCCCACCAGCCACACCGCGATCGTCGCGCGCAGTCTCGCCATCCCCGCGGTGGTGGGCCTGCACCACA
TCCGCGACCTGGTCGAAGACGACGAACTGCTGATCGTCGACGGAACGCGCGGGGTGATCATCGTCGCGCCCGACGACAGC
ATCATCGAGGAATACCGGCTGCGGCGCGCCGAGCTGGAACTCGAACGCTCCAAGCTCAAGCGCCTGCGCGACACCCCGGC
CACCACGCTGGATGGCGAGACCATCCACCTGCTCGCGAACATCGAAGGCCCGAAGGACGTCGCCCAGGTCAAGGCCTCGA
ACGCCGACGGCGTGGGGCTGTACCGCACCGAATTCCTCTTCATCGGGCGCGACACGCTGCCCGACGAAGACGAGCAGTAC
GAGGCCTACCGCACCGTGCTGAAGGCGGTTCCCGGCAAGCCGGTGACCATCCGCACCTTCGATGTCGGCGCGGACAAGGC
GCTCAACGGCGCCCACTCCCGCTTCGAACCCAATCCGGCGCTCGGCCTGCGCGCGGTGCGCTACTCGCTTGCCGAGCCCA
AGATGTTCCTCACCCAGCTGCGCGCCCTGCTGCGCGCCTCGGTGCACGGCCGGCTGCAGATCATGGTGCCGATGCTCGCC
CATGCGCACGAGATCGACCAGACACTGACGCTGATCGACAAGGCCAAGGCCGAGCTGCGCGCCGAGCGCATCAAGTTCGA
CGAGCAGGTTCCGATCGGCGGCATGATCGAAGTGCCGGCGGCGGCACTCGCACTCGGCATGTTCATCCGCCGGCTGTCCT
TCCTGTCGATCGGCACCAACGACCTCATCCAGTACACGCTCGCCATCGACCGCTCGGACGAGGCCGTCGTCCATCTCTAC
GACCCGCTCCATCCGGCGGTGCTCAAGCTGATCGGCGCCACCATCCAGGCCGGCGCCCGCTACGGCCTGCCGGTGTCGGT
GTGCGGCGAGATGGCGGGCGACCCCGCCTACACCTTGCTGCTGCTGGGCATGGGGCTGCGCAACTTCTCGATGCACCCCG
GTCACATCCTCGAGATCAAGCAACAGGTGCTGCGGGCCGACCTGGGGGAGCTGGCGCCGCGCGTGCAGCGCATCCTGAAG
ATGGACGAGCAAGCGCGCGTGCGTGAAGCGGTGGAGCGCCTGGCCGGCTAG

Upstream 100 bases:

>100_bases
CGCCACGGTCACCGTGGAAACCGCCGGCTCCGACGCCGACGCGGCCTTGGAGGCGCTGCTGGCGCTGATCGCCGACAAGT
TCGGCGAAGGGGAATGAGGC

Downstream 100 bases:

>100_bases
CACGGCGTTGCATCGGCATCGCCGCGCCACTAGAATGGCGTCCTTCCTGCGCGGCGGTTCCGCCGCGGCCCCGAGCGGGC
GTCGTATAACGGCTATTACC

Product: putative phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MPFTLHGLPVSQGIAIGHVHLVSHALLEVNHYHVAQRHLADETARLDDAVATVQGELIGLKAAATGGQAHSEVGAFVDLQ
LMMLADPMLIDAARALIESRGCNAEWALVQQMELVVAQFRQIEDPYLRERQADVVQVVERLVKVLLGHPGHLPPRRRDGL
GTIVVAHDLSPADTIGFRDHNIAGFVTDVGGPTSHTAIVARSLAIPAVVGLHHIRDLVEDDELLIVDGTRGVIIVAPDDS
IIEEYRLRRAELELERSKLKRLRDTPATTLDGETIHLLANIEGPKDVAQVKASNADGVGLYRTEFLFIGRDTLPDEDEQY
EAYRTVLKAVPGKPVTIRTFDVGADKALNGAHSRFEPNPALGLRAVRYSLAEPKMFLTQLRALLRASVHGRLQIMVPMLA
HAHEIDQTLTLIDKAKAELRAERIKFDEQVPIGGMIEVPAAALALGMFIRRLSFLSIGTNDLIQYTLAIDRSDEAVVHLY
DPLHPAVLKLIGATIQAGARYGLPVSVCGEMAGDPAYTLLLLGMGLRNFSMHPGHILEIKQQVLRADLGELAPRVQRILK
MDEQARVREAVERLAG

Sequences:

>Translated_576_residues
MPFTLHGLPVSQGIAIGHVHLVSHALLEVNHYHVAQRHLADETARLDDAVATVQGELIGLKAAATGGQAHSEVGAFVDLQ
LMMLADPMLIDAARALIESRGCNAEWALVQQMELVVAQFRQIEDPYLRERQADVVQVVERLVKVLLGHPGHLPPRRRDGL
GTIVVAHDLSPADTIGFRDHNIAGFVTDVGGPTSHTAIVARSLAIPAVVGLHHIRDLVEDDELLIVDGTRGVIIVAPDDS
IIEEYRLRRAELELERSKLKRLRDTPATTLDGETIHLLANIEGPKDVAQVKASNADGVGLYRTEFLFIGRDTLPDEDEQY
EAYRTVLKAVPGKPVTIRTFDVGADKALNGAHSRFEPNPALGLRAVRYSLAEPKMFLTQLRALLRASVHGRLQIMVPMLA
HAHEIDQTLTLIDKAKAELRAERIKFDEQVPIGGMIEVPAAALALGMFIRRLSFLSIGTNDLIQYTLAIDRSDEAVVHLY
DPLHPAVLKLIGATIQAGARYGLPVSVCGEMAGDPAYTLLLLGMGLRNFSMHPGHILEIKQQVLRADLGELAPRVQRILK
MDEQARVREAVERLAG
>Mature_575_residues
PFTLHGLPVSQGIAIGHVHLVSHALLEVNHYHVAQRHLADETARLDDAVATVQGELIGLKAAATGGQAHSEVGAFVDLQL
MMLADPMLIDAARALIESRGCNAEWALVQQMELVVAQFRQIEDPYLRERQADVVQVVERLVKVLLGHPGHLPPRRRDGLG
TIVVAHDLSPADTIGFRDHNIAGFVTDVGGPTSHTAIVARSLAIPAVVGLHHIRDLVEDDELLIVDGTRGVIIVAPDDSI
IEEYRLRRAELELERSKLKRLRDTPATTLDGETIHLLANIEGPKDVAQVKASNADGVGLYRTEFLFIGRDTLPDEDEQYE
AYRTVLKAVPGKPVTIRTFDVGADKALNGAHSRFEPNPALGLRAVRYSLAEPKMFLTQLRALLRASVHGRLQIMVPMLAH
AHEIDQTLTLIDKAKAELRAERIKFDEQVPIGGMIEVPAAALALGMFIRRLSFLSIGTNDLIQYTLAIDRSDEAVVHLYD
PLHPAVLKLIGATIQAGARYGLPVSVCGEMAGDPAYTLLLLGMGLRNFSMHPGHILEIKQQVLRADLGELAPRVQRILKM
DEQARVREAVERLAG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=539, Percent_Identity=39.3320964749536, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1789193, Length=573, Percent_Identity=34.9040139616056, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1788726, Length=551, Percent_Identity=32.3049001814882, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI48994992, Length=417, Percent_Identity=34.2925659472422, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1787994, Length=439, Percent_Identity=26.1958997722096, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI226510935, Length=187, Percent_Identity=26.7379679144385, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63077; Mature: 62946

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFTLHGLPVSQGIAIGHVHLVSHALLEVNHYHVAQRHLADETARLDDAVATVQGELIGL
CCEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
KAAATGGQAHSEVGAFVDLQLMMLADPMLIDAARALIESRGCNAEWALVQQMELVVAQFR
EEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QIEDPYLRERQADVVQVVERLVKVLLGHPGHLPPRRRDGLGTIVVAHDLSPADTIGFRDH
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
NIAGFVTDVGGPTSHTAIVARSLAIPAVVGLHHIRDLVEDDELLIVDGTRGVIIVAPDDS
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCHH
IIEEYRLRRAELELERSKLKRLRDTPATTLDGETIHLLANIEGPKDVAQVKASNADGVGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCEE
YRTEFLFIGRDTLPDEDEQYEAYRTVLKAVPGKPVTIRTFDVGADKALNGAHSRFEPNPA
EEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCCCCCHH
LGLRAVRYSLAEPKMFLTQLRALLRASVHGRLQIMVPMLAHAHEIDQTLTLIDKAKAELR
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AERIKFDEQVPIGGMIEVPAAALALGMFIRRLSFLSIGTNDLIQYTLAIDRSDEAVVHLY
HHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCEEEEEE
DPLHPAVLKLIGATIQAGARYGLPVSVCGEMAGDPAYTLLLLGMGLRNFSMHPGHILEIK
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
QQVLRADLGELAPRVQRILKMDEQARVREAVERLAG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFTLHGLPVSQGIAIGHVHLVSHALLEVNHYHVAQRHLADETARLDDAVATVQGELIGL
CEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
KAAATGGQAHSEVGAFVDLQLMMLADPMLIDAARALIESRGCNAEWALVQQMELVVAQFR
EEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QIEDPYLRERQADVVQVVERLVKVLLGHPGHLPPRRRDGLGTIVVAHDLSPADTIGFRDH
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
NIAGFVTDVGGPTSHTAIVARSLAIPAVVGLHHIRDLVEDDELLIVDGTRGVIIVAPDDS
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCHH
IIEEYRLRRAELELERSKLKRLRDTPATTLDGETIHLLANIEGPKDVAQVKASNADGVGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCEE
YRTEFLFIGRDTLPDEDEQYEAYRTVLKAVPGKPVTIRTFDVGADKALNGAHSRFEPNPA
EEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCCCCCHH
LGLRAVRYSLAEPKMFLTQLRALLRASVHGRLQIMVPMLAHAHEIDQTLTLIDKAKAELR
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AERIKFDEQVPIGGMIEVPAAALALGMFIRRLSFLSIGTNDLIQYTLAIDRSDEAVVHLY
HHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCEEEEEE
DPLHPAVLKLIGATIQAGARYGLPVSVCGEMAGDPAYTLLLLGMGLRNFSMHPGHILEIK
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
QQVLRADLGELAPRVQRILKMDEQARVREAVERLAG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]