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The map label for this gene is dosC [H]

Identifier: 119900066

GI number: 119900066

Start: 4138598

End: 4140025

Strand: Direct

Name: dosC [H]

Synonym: azo3777

Alternate gene names: 119900066

Gene position: 4138598-4140025 (Clockwise)

Preceding gene: 119900064

Following gene: 119900067

Centisome position: 94.57

GC content: 67.58

Gene sequence:

>1428_bases
ATGCGCCTCTTTCCCGCACGGTTTTCCGCGATGTCCTCCGCCGGTCATTTCTCGCACGAAACCATCGAATCCGACTGGTT
GAAGCTGTTCGACGCCGTTTCCCCCTCGCTGCGCGAAGCGGTCCGCGACGTCGTGCTCGAACATCGCGCGGCGATGGCGG
ACGCCTTCTACGCCCGCATGATGGCCGACGGCGACGCCCGGCCGTTTCTTTCCCACGAAGCGGTGCACCAGCGCCTGCGG
TTCTCGATGCAGCGCTGGATGGAAACCCTGTTCTCGTGCGCGGACCACGCCGCCTTCGCCGCCGCGATGGCGCTCCAGCG
CCATGTCGGCGAAGTGCATTCGCGCGTCGATCTGCCGGTGAACCTCGTCGCGCGCGGCGCCCGCGTGCTGAAGGGCGAGA
TCGCCGCCCGGCTGCCGGCGAGCGGGCTCGACCGCGCCGCGCTGGTGCAGGCGGTGCTGTACGTGGACAACCTGATCGAC
CTCGCCTTCGAGGTCATGAGTTCGGCCTATGTCGCCTCGCATGAACGCGCCACGCGCATCGACGAGGCCTACCGGGTGTT
CTCCTACGGCCAGAACATGTCGCTGGAACGCGAACGCCAGCGCGCCTCGCTGCTCGACTGGGAGAACCGCTTCCTGCAGG
CGATGATGGCCGGCCAGAGCGGCGACGAACTGCCGTTGATCAGCGATTCCGCATTCGGCCTGTGGCTGCAACACAAGGCG
GTGGCGGTGTTCGAGGGCTCGGCCGAGCTGCCGTCGATACGCGAAGGCATGCGCCGGCTCGACCGCGATCTGATCCCGCT
GTGCCGGCAGCAGATGGAAAGCCTGGACGAGCGCGGCGAGAGCCGGCGGCTGATCAAGGCGGTGCAGTCCGAACTCGGCC
AGCTCAAGTTCCTGATCGGCGCGATGTTCGAACGCTTCGTCGATCTGGAGTCGGGCAAGGACGCGCTCACCCAGCTGCTC
AACCGCCGCTTCCTGCCGGCGATCCTGACGCGCGAGACCGAGATCGCGCGCAACCAGCACAAGTCCTTCGCACTGCTGCT
GGTCGATGTCGACCACTTCAAGCAGATCAACGACGTCCACGGTCACGACGCCGGCGACCGCGTGCTGCAGCAGATCGCCA
GCCTGTTGCTCAACACCGTGCGTTCGGGCGACTTCGTGTTCCGCTACGGCGGCGAGGAGTTCCTGGTGCTGCTGGTCGAG
GTGGACCGCCAGCACGCGGCGCGCGTCGCCGAGAAGATCCGCCAGCGGGTGCAGCAGGAAACCTTCCTGCTGCCCGACAG
CCGGCAGCTTCAGGTCACCGCCAGCGTCGGCGTGGCGCTGCACGACGGCCACCCGGACTACCAGCGCCTGATCAACCAGG
CCGACGACGCGCTCTACCGCGCCAAGACCGGCGGCCGCAACCGCTGCGAGTTCGCCACGGACGCCTGA

Upstream 100 bases:

>100_bases
CACGCATCGCGAATCCTGCAGGACGTTTGTTTCCATTCTATGCGTAGCGGGAGCCGGCCGAGAAGCCGGCGCCCTGTTAC
AAGTCGCGACGCGTGGCACA

Downstream 100 bases:

>100_bases
ACAATGCCCACCCCATCCATCCGCATTGAAGCACTGCGCCTTGACGACCCCGCCCACGCCAACGCCTTCCTGGCGCTGCT
CGATCACTACGCGTGCGACC

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MRLFPARFSAMSSAGHFSHETIESDWLKLFDAVSPSLREAVRDVVLEHRAAMADAFYARMMADGDARPFLSHEAVHQRLR
FSMQRWMETLFSCADHAAFAAAMALQRHVGEVHSRVDLPVNLVARGARVLKGEIAARLPASGLDRAALVQAVLYVDNLID
LAFEVMSSAYVASHERATRIDEAYRVFSYGQNMSLERERQRASLLDWENRFLQAMMAGQSGDELPLISDSAFGLWLQHKA
VAVFEGSAELPSIREGMRRLDRDLIPLCRQQMESLDERGESRRLIKAVQSELGQLKFLIGAMFERFVDLESGKDALTQLL
NRRFLPAILTRETEIARNQHKSFALLLVDVDHFKQINDVHGHDAGDRVLQQIASLLLNTVRSGDFVFRYGGEEFLVLLVE
VDRQHAARVAEKIRQRVQQETFLLPDSRQLQVTASVGVALHDGHPDYQRLINQADDALYRAKTGGRNRCEFATDA

Sequences:

>Translated_475_residues
MRLFPARFSAMSSAGHFSHETIESDWLKLFDAVSPSLREAVRDVVLEHRAAMADAFYARMMADGDARPFLSHEAVHQRLR
FSMQRWMETLFSCADHAAFAAAMALQRHVGEVHSRVDLPVNLVARGARVLKGEIAARLPASGLDRAALVQAVLYVDNLID
LAFEVMSSAYVASHERATRIDEAYRVFSYGQNMSLERERQRASLLDWENRFLQAMMAGQSGDELPLISDSAFGLWLQHKA
VAVFEGSAELPSIREGMRRLDRDLIPLCRQQMESLDERGESRRLIKAVQSELGQLKFLIGAMFERFVDLESGKDALTQLL
NRRFLPAILTRETEIARNQHKSFALLLVDVDHFKQINDVHGHDAGDRVLQQIASLLLNTVRSGDFVFRYGGEEFLVLLVE
VDRQHAARVAEKIRQRVQQETFLLPDSRQLQVTASVGVALHDGHPDYQRLINQADDALYRAKTGGRNRCEFATDA
>Mature_475_residues
MRLFPARFSAMSSAGHFSHETIESDWLKLFDAVSPSLREAVRDVVLEHRAAMADAFYARMMADGDARPFLSHEAVHQRLR
FSMQRWMETLFSCADHAAFAAAMALQRHVGEVHSRVDLPVNLVARGARVLKGEIAARLPASGLDRAALVQAVLYVDNLID
LAFEVMSSAYVASHERATRIDEAYRVFSYGQNMSLERERQRASLLDWENRFLQAMMAGQSGDELPLISDSAFGLWLQHKA
VAVFEGSAELPSIREGMRRLDRDLIPLCRQQMESLDERGESRRLIKAVQSELGQLKFLIGAMFERFVDLESGKDALTQLL
NRRFLPAILTRETEIARNQHKSFALLLVDVDHFKQINDVHGHDAGDRVLQQIASLLLNTVRSGDFVFRYGGEEFLVLLVE
VDRQHAARVAEKIRQRVQQETFLLPDSRQLQVTASVGVALHDGHPDYQRLINQADDALYRAKTGGRNRCEFATDA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=454, Percent_Identity=36.3436123348018, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=44.6540880503145, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=39.5209580838323, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=35.8823529411765, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1787262, Length=158, Percent_Identity=36.0759493670886, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788381, Length=166, Percent_Identity=36.7469879518072, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=36.4705882352941, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787816, Length=253, Percent_Identity=28.0632411067194, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788085, Length=190, Percent_Identity=32.1052631578947, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081974, Length=169, Percent_Identity=30.1775147928994, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=30.1775147928994, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=154, Percent_Identity=32.4675324675325, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788956, Length=150, Percent_Identity=34, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=124, Percent_Identity=33.8709677419355, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 53671; Mature: 53671

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFPARFSAMSSAGHFSHETIESDWLKLFDAVSPSLREAVRDVVLEHRAAMADAFYARM
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MADGDARPFLSHEAVHQRLRFSMQRWMETLFSCADHAAFAAAMALQRHVGEVHSRVDLPV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
NLVARGARVLKGEIAARLPASGLDRAALVQAVLYVDNLIDLAFEVMSSAYVASHERATRI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAYRVFSYGQNMSLERERQRASLLDWENRFLQAMMAGQSGDELPLISDSAFGLWLQHKA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHCCE
VAVFEGSAELPSIREGMRRLDRDLIPLCRQQMESLDERGESRRLIKAVQSELGQLKFLIG
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AMFERFVDLESGKDALTQLLNRRFLPAILTRETEIARNQHKSFALLLVDVDHFKQINDVH
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCC
GHDAGDRVLQQIASLLLNTVRSGDFVFRYGGEEFLVLLVEVDRQHAARVAEKIRQRVQQE
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
TFLLPDSRQLQVTASVGVALHDGHPDYQRLINQADDALYRAKTGGRNRCEFATDA
HCCCCCCCCEEEEEEECEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLFPARFSAMSSAGHFSHETIESDWLKLFDAVSPSLREAVRDVVLEHRAAMADAFYARM
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MADGDARPFLSHEAVHQRLRFSMQRWMETLFSCADHAAFAAAMALQRHVGEVHSRVDLPV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
NLVARGARVLKGEIAARLPASGLDRAALVQAVLYVDNLIDLAFEVMSSAYVASHERATRI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAYRVFSYGQNMSLERERQRASLLDWENRFLQAMMAGQSGDELPLISDSAFGLWLQHKA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHCCE
VAVFEGSAELPSIREGMRRLDRDLIPLCRQQMESLDERGESRRLIKAVQSELGQLKFLIG
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AMFERFVDLESGKDALTQLLNRRFLPAILTRETEIARNQHKSFALLLVDVDHFKQINDVH
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCC
GHDAGDRVLQQIASLLLNTVRSGDFVFRYGGEEFLVLLVEVDRQHAARVAEKIRQRVQQE
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
TFLLPDSRQLQVTASVGVALHDGHPDYQRLINQADDALYRAKTGGRNRCEFATDA
HCCCCCCCCEEEEEEECEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]