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The map label for this gene is lutR [H]

Identifier: 119866404

GI number: 119866404

Start: 383430

End: 384095

Strand: Reverse

Name: lutR [H]

Synonym: Mkms_0349

Alternate gene names: 119866404

Gene position: 384095-383430 (Counterclockwise)

Preceding gene: 119866406

Following gene: 119866402

Centisome position: 6.69

GC content: 70.57

Gene sequence:

>666_bases
GTGCCGCTCGCCACTACCCGCCGCGCAGGCTTGGTGGACCAGGTGATCGCTCAACTGCGCGCGTCGGTGTCCGCCGGCGA
GTGGCCGGTCGGCACCCGGATCCCGACGGAGCCCGCACTGGTCGCCGCTCTCGGCGTCGGCCGCAACACGGTCCGGGAAG
CGGTGCGCGCGCTGGCCCACAGCGGAATCCTGGAGGTCCGACAGGGCGACGGCACATATGTGCGGGCCACGAGCGAGGTC
TCCGGGGCAGTGCGCCGACTGTGCGGTTCGGAGTTGCGCGAGGTGCTGGAGGTGCGGCGTTGCCTCGAGGTCGAAGGTGC
CCGGTTGGCCGCGACCGCGCGCACCGCCGAAGACCTTGCCGAGATTCGCGCGACGCTCGCCCGCCGGGATGCCCTCGACG
CCCGTGACGAGTTCGTGCGGGCCGACGCGGAATTCCACTTCGCGGTGGTGAGGGCGTCGCACAACGCGGTGCTGACCGAG
TTGTACCGCGGGCTCACCGAAGTGGTGACCGCGAGTGTGGCGACCACCGTGGAAACCCACGCATTCGACCGGATCCCGCA
CGACGGGCTGGTCGAAGCGATCGCCAGAGGCGATGTCGACCGAGCCGGCCACGAGGCAGGCGGTTTCCTCGAGGAGCTCA
TCGAGGAACTACCGGATCGACGCTGA

Upstream 100 bases:

>100_bases
GCCGGCTTCGGACGCCTGACCGGCTGTGGCGTCGACGCGCGTGTGGCCCTTCACCGCGGTATCGTCCCATACATCCCATG
ATTGGACGAATGGAGTTTCG

Downstream 100 bases:

>100_bases
GGCGGACCGGCGCGTGCAACACGCTGTGCTGCATGGCTATTCGGGCTCGTCGTCTTGACCGTCGTCGTTGTCGGTGAGGT
AGCGGTGGGGGTGGAAGTGG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MPLATTRRAGLVDQVIAQLRASVSAGEWPVGTRIPTEPALVAALGVGRNTVREAVRALAHSGILEVRQGDGTYVRATSEV
SGAVRRLCGSELREVLEVRRCLEVEGARLAATARTAEDLAEIRATLARRDALDARDEFVRADAEFHFAVVRASHNAVLTE
LYRGLTEVVTASVATTVETHAFDRIPHDGLVEAIARGDVDRAGHEAGGFLEELIEELPDRR

Sequences:

>Translated_221_residues
MPLATTRRAGLVDQVIAQLRASVSAGEWPVGTRIPTEPALVAALGVGRNTVREAVRALAHSGILEVRQGDGTYVRATSEV
SGAVRRLCGSELREVLEVRRCLEVEGARLAATARTAEDLAEIRATLARRDALDARDEFVRADAEFHFAVVRASHNAVLTE
LYRGLTEVVTASVATTVETHAFDRIPHDGLVEAIARGDVDRAGHEAGGFLEELIEELPDRR
>Mature_220_residues
PLATTRRAGLVDQVIAQLRASVSAGEWPVGTRIPTEPALVAALGVGRNTVREAVRALAHSGILEVRQGDGTYVRATSEVS
GAVRRLCGSELREVLEVRRCLEVEGARLAATARTAEDLAEIRATLARRDALDARDEFVRADAEFHFAVVRASHNAVLTEL
YRGLTEVVTASVATTVETHAFDRIPHDGLVEAIARGDVDRAGHEAGGFLEELIEELPDRR

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=208, Percent_Identity=29.8076923076923, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790780, Length=157, Percent_Identity=33.1210191082803, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994961, Length=218, Percent_Identity=29.3577981651376, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1789353, Length=165, Percent_Identity=32.1212121212121, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786303, Length=235, Percent_Identity=28.936170212766, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789621, Length=215, Percent_Identity=26.046511627907, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI48994955, Length=205, Percent_Identity=27.3170731707317, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 23878; Mature: 23747

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLATTRRAGLVDQVIAQLRASVSAGEWPVGTRIPTEPALVAALGVGRNTVREAVRALAH
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
SGILEVRQGDGTYVRATSEVSGAVRRLCGSELREVLEVRRCLEVEGARLAATARTAEDLA
CCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EIRATLARRDALDARDEFVRADAEFHFAVVRASHNAVLTELYRGLTEVVTASVATTVETH
HHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDRIPHDGLVEAIARGDVDRAGHEAGGFLEELIEELPDRR
HHHCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLATTRRAGLVDQVIAQLRASVSAGEWPVGTRIPTEPALVAALGVGRNTVREAVRALAH
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
SGILEVRQGDGTYVRATSEVSGAVRRLCGSELREVLEVRRCLEVEGARLAATARTAEDLA
CCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EIRATLARRDALDARDEFVRADAEFHFAVVRASHNAVLTELYRGLTEVVTASVATTVETH
HHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDRIPHDGLVEAIARGDVDRAGHEAGGFLEELIEELPDRR
HHHCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]