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The map label for this gene is hcaE [H]

Identifier: 119855233

GI number: 119855233

Start: 54861

End: 56264

Strand: Reverse

Name: hcaE [H]

Synonym: Mkms_5847

Alternate gene names: 119855233

Gene position: 56264-54861 (Counterclockwise)

Preceding gene: 119855234

Following gene: 119855232

Centisome position: 25.96

GC content: 58.69

Gene sequence:

>1404_bases
ATGAGTACTTCAGGCGGTTCGGACACGACCGCTCGTAGTCTCGTCGATGTCGATCGCGGGGAGATCAGTCGCGAAATCTT
CACCGATGCTTCGATTTTCAACCGTGAGTTGGAGTATTTGTTTCCGCGGAGCTGGTTGTTTGTGGGTCATGCCTCGCAGG
TGTCTGCGCCGGGTCAGTTCTTCTCGTCGAGGATGGGCTCGGATCCGGTGTTGTTGACCCGTGACGCTCAGGGTGGTGTG
AATGTCTTGCTCAATTCGTGCCGACATCGGGGTATGGCGGTGTGTCGCTATGACGAGGGCCGTACGCTGCAGTTCACCTG
TCCCTATCATGGCTGGTCGTACTCGATGGACGGTTCGCTGGTGTCCACTCCAGGGGATTTGCACGGTGTGCCGCAGCAGG
GCATGGCCTACGGCAACGGCCTTGACAAAGCGGCCTGGGGACTTGTCAGGGCTGCCAAGGTGCACAATTACAAGGGCCTG
GTATTCGCGTGCTGGGATCCATCAGCCCCGGAGTTCGATGAATACGTTGGGGACTTTCATCATTGGCTGGATAACCTGTC
TGATGCTTTTGATGGTACGGAAGGTGGTACCGAGGTGTTCCGTGGGGTGCAGAAGTGGCGCATCAAATCAAATTGGAAAT
TCGTCTCAGAGAATTTCTTGGGCGATACCTATCACGGGGCGACGACCCATGCCTCGGTTGAACAGGTGGGCATCGGGCCG
GGTGGCAGAAATTCACGACGTCACGGTGAACGACAGGATCAGGGTGGTTTTTCGAAGGGCCGTGTGAAGACGTCGTTTCG
GATGGGCCATGGCGCGTCGGACAATCTGGCGTATGAGATTCCCTATCCTGAGTTCGCCGAAGAACCGGCCTTGAGTGAGT
ACTTCTCCCAGGCGTGGGCGGTCCGCAAGGAGCGACTGCAGGCGCAGGGCAGACTGCTCGGTGGTCGTGGCCCGGCGACG
ATGTTCCCCAATATGTCGTTTTCGGCCGGTTTTCCGCGGACGATCCTGGTGTCACATCCGATCAGCCCGACCGAAACCGA
GGTGTGGCGCTGGTATCTCGTCGACAAGAACGCACCCGATGATGTGCGTGACTGGTTGCGCCGCTATTACATGCGCTACT
CGGGTCCTGGAGGGATGACGGAGCAAGACGATATGGAGAACTGGAATTACGCGACGCAGGCCAGCCAGGGCGTGATAGCC
CGGCGCTACCCCTACAACTATCAGCAGGGTCTCGGCAAGGAAACTCCCAGTGAGCTCGACCAGGCGGTGCATTCTCACCA
GCCCATCGCTGGCGAGGTGAATGCACGCGCCTTTTACCGGCGATGGGCCGAGTTCACCGACAACCTCTCGTGGCCCCTAC
TCATCGAACTCGCCAAATCCGACGAGAGAGCCGCACGGTCGTGA

Upstream 100 bases:

>100_bases
TGCCCGGCAGACGCGGTGACGGTTTGTGGTGATTTGAGTTACGGCTGTCTCGGGGTGATTCGGGCATTACAGCAAGGGAA
AGGTACCGATGACGTTAAAA

Downstream 100 bases:

>100_bases
GTGCAACGGGCGACGAAATTTCCACGAGGCAGGACGCGGTAGAAAAGTTGCTGCTACAGGCCGAAATCGCCGACTTTCTT
TATCGGGAAGCCGACCTGCT

Product: ring hydroxylating dioxygenase, alpha subunit

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSTSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSAPGQFFSSRMGSDPVLLTRDAQGGV
NVLLNSCRHRGMAVCRYDEGRTLQFTCPYHGWSYSMDGSLVSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHNYKGL
VFACWDPSAPEFDEYVGDFHHWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQVGIGP
GGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFSQAWAVRKERLQAQGRLLGGRGPAT
MFPNMSFSAGFPRTILVSHPISPTETEVWRWYLVDKNAPDDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIA
RRYPYNYQQGLGKETPSELDQAVHSHQPIAGEVNARAFYRRWAEFTDNLSWPLLIELAKSDERAARS

Sequences:

>Translated_467_residues
MSTSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSAPGQFFSSRMGSDPVLLTRDAQGGV
NVLLNSCRHRGMAVCRYDEGRTLQFTCPYHGWSYSMDGSLVSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHNYKGL
VFACWDPSAPEFDEYVGDFHHWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQVGIGP
GGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFSQAWAVRKERLQAQGRLLGGRGPAT
MFPNMSFSAGFPRTILVSHPISPTETEVWRWYLVDKNAPDDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIA
RRYPYNYQQGLGKETPSELDQAVHSHQPIAGEVNARAFYRRWAEFTDNLSWPLLIELAKSDERAARS
>Mature_466_residues
STSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSAPGQFFSSRMGSDPVLLTRDAQGGVN
VLLNSCRHRGMAVCRYDEGRTLQFTCPYHGWSYSMDGSLVSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHNYKGLV
FACWDPSAPEFDEYVGDFHHWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQVGIGPG
GRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFSQAWAVRKERLQAQGRLLGGRGPATM
FPNMSFSAGFPRTILVSHPISPTETEVWRWYLVDKNAPDDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIAR
RYPYNYQQGLGKETPSELDQAVHSHQPIAGEVNARAFYRRWAEFTDNLSWPLLIELAKSDERAARS

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=452, Percent_Identity=37.6106194690265, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1788103, Length=259, Percent_Identity=25.8687258687259, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 52458; Mature: 52326

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSAPGQF
CCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
FSSRMGSDPVLLTRDAQGGVNVLLNSCRHRGMAVCRYDEGRTLQFTCPYHGWSYSMDGSL
HHHHCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCEECCCCE
VSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHNYKGLVFACWDPSAPEFDEYVGDFH
ECCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
HWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQVGIGP
HHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCEEEHHCCCCCCCCCCCCCCCHHHCCCCC
GGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFSQAWA
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCHHCCCCHHHHHHHHHHH
VRKERLQAQGRLLGGRGPATMFPNMSFSAGFPRTILVSHPISPTETEVWRWYLVDKNAPD
HHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCH
DVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIARRYPYNYQQGLGKETPSELD
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHCCCCCCHHHHH
QAVHSHQPIAGEVNARAFYRRWAEFTDNLSWPLLIELAKSDERAARS
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCC
>Mature Secondary Structure 
STSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSAPGQF
CCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
FSSRMGSDPVLLTRDAQGGVNVLLNSCRHRGMAVCRYDEGRTLQFTCPYHGWSYSMDGSL
HHHHCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCEECCCCE
VSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHNYKGLVFACWDPSAPEFDEYVGDFH
ECCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
HWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQVGIGP
HHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCEEEHHCCCCCCCCCCCCCCCHHHCCCCC
GGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFSQAWA
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCHHCCCCHHHHHHHHHHH
VRKERLQAQGRLLGGRGPATMFPNMSFSAGFPRTILVSHPISPTETEVWRWYLVDKNAPD
HHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCH
DVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIARRYPYNYQQGLGKETPSELD
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHCCCCCCHHHHH
QAVHSHQPIAGEVNARAFYRRWAEFTDNLSWPLLIELAKSDERAARS
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]