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The map label for this gene is xylX [H]

Identifier: 119855022

GI number: 119855022

Start: 142545

End: 143726

Strand: Direct

Name: xylX [H]

Synonym: Mkms_5630

Alternate gene names: 119855022

Gene position: 142545-143726 (Clockwise)

Preceding gene: 119855021

Following gene: 119855023

Centisome position: 47.19

GC content: 63.62

Gene sequence:

>1182_bases
GTGAAGCTTGGACCGACGATGGAGTTCGACCACATACAGCCGGCGATCCGGAAACGGTTCACCTCTGCAGCGGACATCCC
CAAGGAGGTGTTCAGCGACCCCGACGTCTACCGGGAAGAGCTCACCCGCATCTTCTATGGCCCCTACTGGCATCCGATCG
CGCACCGGGCCGAGCTGGCCGAGCGCAACGCCTTCCGGACGAGATGGCTGGCCGACGTGCCGCTGCTGATGGTGCGGGAC
GGCGATGACCGCATCCGCGTGTTCGTCAACTCCTGCGCCCATCGGGGAACGCTACTGGAACAGCGCCGGTGCGGGGTGGC
GGAGCGATTCGAGTGTCCGTATCACCGGTGGATCTTCAACAATGACGGCCGTTTCGCCGGCGCGCCCCGCCGCATGCAGT
TTCGCCCGGACTTTCGCGAGGAGGACTACGGCCTCCGGGAGCTGCACGTAGTCGAGGCGTGGGGTTTGATCTTCGTCAGC
ATGGCTGCGCAGCCGCCGCCGTTCGACGATTATCTCGGCGATAGCGCGGATCCGTTGCGCGACTGCATGGTCGATGACGG
GGACTTGACGTTGCTGGGCTACCAGACGGTGGTGTTTCAGAGTAATTGGAAAACCTACATCGACAACGATCCCTATCACG
CGCCGCTGCTGCACAGCGCGTTCAAACTGCTCAACTGGCAGGGCGGCAGCGGAAACGTGCTGGTCAGCGAGCCCTATGGG
CACATGTCGATTCTGTACGATGCGCAACCCTACGTGGACAACGGTTTCCTGGCTGACCCGAGTGTGGTCACGCGGATGGG
GGATGACAGCCGAGCCCGCGTGATTGCGTTACGGCCGGTTACCGGGATCGTGCGTCACGTCGACACGATCAACGTCCGGT
ACGCCCGCCCGCTGGGGGTTGATCGTACCGAGGTGCGATACACGTTCTTCGGCCATGCCAGTGACTCCGAGGACTTCGCA
CGCCACCGAGTCCGCCAGTCGTCAAACCTGCTGGGGCCGAGCGGCTTCATCAGTATCGAGGACGCCGCCGTCTACAACCG
CGTGCAGGCGACCGCGCGTGACGGCGGCTATCAGCGCTTTGTCGCCGGCGTCGGCCGACCATTGTCGGAGTCGTCGCAGA
ACGACGAGGTCGCCAATACCGGCTGGTGGGCGCACTACCAGGAGGTGATGGAGTTTTGTTGA

Upstream 100 bases:

>100_bases
GGCTTTACGTGTCGGAAGACGAGGGCCACACCTGGAAGTGCCTGGCCGACGGCCTGGGCGCGATCACCCACATCGCGACG
CCGAAGAAGGGAACAGCGCA

Downstream 100 bases:

>100_bases
CATCGAGCGTCGAAGATCGGCGGGCACGTGCCGCGTACCTGGTGGACGAACTGCTGGGAGCCTACGCCCGCGCAGTCGAC
GATCAAGACTTCACCGCGTG

Product: Rieske (2Fe-2S) domain-containing protein

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKLGPTMEFDHIQPAIRKRFTSAADIPKEVFSDPDVYREELTRIFYGPYWHPIAHRAELAERNAFRTRWLADVPLLMVRD
GDDRIRVFVNSCAHRGTLLEQRRCGVAERFECPYHRWIFNNDGRFAGAPRRMQFRPDFREEDYGLRELHVVEAWGLIFVS
MAAQPPPFDDYLGDSADPLRDCMVDDGDLTLLGYQTVVFQSNWKTYIDNDPYHAPLLHSAFKLLNWQGGSGNVLVSEPYG
HMSILYDAQPYVDNGFLADPSVVTRMGDDSRARVIALRPVTGIVRHVDTINVRYARPLGVDRTEVRYTFFGHASDSEDFA
RHRVRQSSNLLGPSGFISIEDAAVYNRVQATARDGGYQRFVAGVGRPLSESSQNDEVANTGWWAHYQEVMEFC

Sequences:

>Translated_393_residues
MKLGPTMEFDHIQPAIRKRFTSAADIPKEVFSDPDVYREELTRIFYGPYWHPIAHRAELAERNAFRTRWLADVPLLMVRD
GDDRIRVFVNSCAHRGTLLEQRRCGVAERFECPYHRWIFNNDGRFAGAPRRMQFRPDFREEDYGLRELHVVEAWGLIFVS
MAAQPPPFDDYLGDSADPLRDCMVDDGDLTLLGYQTVVFQSNWKTYIDNDPYHAPLLHSAFKLLNWQGGSGNVLVSEPYG
HMSILYDAQPYVDNGFLADPSVVTRMGDDSRARVIALRPVTGIVRHVDTINVRYARPLGVDRTEVRYTFFGHASDSEDFA
RHRVRQSSNLLGPSGFISIEDAAVYNRVQATARDGGYQRFVAGVGRPLSESSQNDEVANTGWWAHYQEVMEFC
>Mature_393_residues
MKLGPTMEFDHIQPAIRKRFTSAADIPKEVFSDPDVYREELTRIFYGPYWHPIAHRAELAERNAFRTRWLADVPLLMVRD
GDDRIRVFVNSCAHRGTLLEQRRCGVAERFECPYHRWIFNNDGRFAGAPRRMQFRPDFREEDYGLRELHVVEAWGLIFVS
MAAQPPPFDDYLGDSADPLRDCMVDDGDLTLLGYQTVVFQSNWKTYIDNDPYHAPLLHSAFKLLNWQGGSGNVLVSEPYG
HMSILYDAQPYVDNGFLADPSVVTRMGDDSRARVIALRPVTGIVRHVDTINVRYARPLGVDRTEVRYTFFGHASDSEDFA
RHRVRQSSNLLGPSGFISIEDAAVYNRVQATARDGGYQRFVAGVGRPLSESSQNDEVANTGWWAHYQEVMEFC

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=367, Percent_Identity=26.7029972752044, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788103, Length=360, Percent_Identity=25.8333333333333, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 44993; Mature: 44993

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGPTMEFDHIQPAIRKRFTSAADIPKEVFSDPDVYREELTRIFYGPYWHPIAHRAELA
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
ERNAFRTRWLADVPLLMVRDGDDRIRVFVNSCAHRGTLLEQRRCGVAERFECPYHRWIFN
HHHHHHHHHHCCCCEEEEECCCCCEEHEEHHHHHCCHHHHHHHCCCHHHCCCCCEEEEEC
NDGRFAGAPRRMQFRPDFREEDYGLRELHVVEAWGLIFVSMAAQPPPFDDYLGDSADPLR
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHH
DCMVDDGDLTLLGYQTVVFQSNWKTYIDNDPYHAPLLHSAFKLLNWQGGSGNVLVSEPYG
HHHCCCCCEEEEEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCC
HMSILYDAQPYVDNGFLADPSVVTRMGDDSRARVIALRPVTGIVRHVDTINVRYARPLGV
CEEEEECCCCCCCCCCCCCCHHHHCCCCCCCEEEEEEECHHHHHHHHHEEEEEEECCCCC
DRTEVRYTFFGHASDSEDFARHRVRQSSNLLGPSGFISIEDAAVYNRVQATARDGGYQRF
CCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHH
VAGVGRPLSESSQNDEVANTGWWAHYQEVMEFC
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MKLGPTMEFDHIQPAIRKRFTSAADIPKEVFSDPDVYREELTRIFYGPYWHPIAHRAELA
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
ERNAFRTRWLADVPLLMVRDGDDRIRVFVNSCAHRGTLLEQRRCGVAERFECPYHRWIFN
HHHHHHHHHHCCCCEEEEECCCCCEEHEEHHHHHCCHHHHHHHCCCHHHCCCCCEEEEEC
NDGRFAGAPRRMQFRPDFREEDYGLRELHVVEAWGLIFVSMAAQPPPFDDYLGDSADPLR
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHH
DCMVDDGDLTLLGYQTVVFQSNWKTYIDNDPYHAPLLHSAFKLLNWQGGSGNVLVSEPYG
HHHCCCCCEEEEEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCC
HMSILYDAQPYVDNGFLADPSVVTRMGDDSRARVIALRPVTGIVRHVDTINVRYARPLGV
CEEEEECCCCCCCCCCCCCCHHHHCCCCCCCEEEEEEECHHHHHHHHHEEEEEEECCCCC
DRTEVRYTFFGHASDSEDFARHRVRQSSNLLGPSGFISIEDAAVYNRVQATARDGGYQRF
CCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHH
VAGVGRPLSESSQNDEVANTGWWAHYQEVMEFC
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]