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The map label for this gene is bphE [H]

Identifier: 119855013

GI number: 119855013

Start: 132662

End: 133171

Strand: Direct

Name: bphE [H]

Synonym: Mkms_5621

Alternate gene names: 119855013

Gene position: 132662-133171 (Clockwise)

Preceding gene: 119855012

Following gene: 119855014

Centisome position: 43.91

GC content: 64.51

Gene sequence:

>510_bases
ATGAACGCGGTTGCGGTCGATCGAGATACCCGTGAGGCCGTCGAGGCGTTCATGTTCCGGGAGGCGGAGCTACTCGATGG
GGGGCAGTTCCGGGAATGGTTGGGTCTGCTCGACCCCGACATCCGGTATGTGGTTCCGGTGCGCACCACCCGTGAGGATT
CCGCGGGTTGGGTGGGCGCGATCGCGCACTGGAACGACGACTACACGGGTTTGGAGATGCGGGTCCTCCGGGGCGAGACG
GATTTCTCGTGGGCCGAGTCACCTCGGTCGCGGACCCGGCACTTCGTGTCCAACATCCGCACGGTTGCCGGACCGGAGGC
TGATGAGTTGACCGTGCGCTCAAATCTCTTGTTCTTCCGCAGCCGCGGAGATAGCGGCCGGTGGGAGTTGCTCTCGGCCG
AACGCGTCGACGTGTTGCGCCGGACCGACGATTCGCTGCGGCTCGCTCGGCGCGAGGTATTGCTCGACCACTCGACGTTG
CCTATCGACAACCTGTCGGTAGTCCTGTAG

Upstream 100 bases:

>100_bases
GGCCCGGTGAGATGTGAATTACATTCTCTACAGCGGAGGATGCATCTGTTCAGCAGAATATCGAGTCAGTGTTGATGGTC
TGGTCGAGGAGTTCGGTGTG

Downstream 100 bases:

>100_bases
GGCCAGCTCCGTTCACCACCTCCGCGTGGATATCGCCGATTCCAGAAAGGGTCCAACCATATGACCACCGAAACAACCGA
GACGGACTTGATCGAGATGT

Product: aromatic-ring-hydroxylating dioxygenase, beta subunit

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MNAVAVDRDTREAVEAFMFREAELLDGGQFREWLGLLDPDIRYVVPVRTTREDSAGWVGAIAHWNDDYTGLEMRVLRGET
DFSWAESPRSRTRHFVSNIRTVAGPEADELTVRSNLLFFRSRGDSGRWELLSAERVDVLRRTDDSLRLARREVLLDHSTL
PIDNLSVVL

Sequences:

>Translated_169_residues
MNAVAVDRDTREAVEAFMFREAELLDGGQFREWLGLLDPDIRYVVPVRTTREDSAGWVGAIAHWNDDYTGLEMRVLRGET
DFSWAESPRSRTRHFVSNIRTVAGPEADELTVRSNLLFFRSRGDSGRWELLSAERVDVLRRTDDSLRLARREVLLDHSTL
PIDNLSVVL
>Mature_169_residues
MNAVAVDRDTREAVEAFMFREAELLDGGQFREWLGLLDPDIRYVVPVRTTREDSAGWVGAIAHWNDDYTGLEMRVLRGET
DFSWAESPRSRTRHFVSNIRTVAGPEADELTVRSNLLFFRSRGDSGRWELLSAERVDVLRRTDDSLRLARREVLLDHSTL
PIDNLSVVL

Specific function: The beta subunit may be responsible for the substrate specificity of the enzyme [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788889, Length=161, Percent_Identity=36.6459627329193, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 19389; Mature: 19389

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVAVDRDTREAVEAFMFREAELLDGGQFREWLGLLDPDIRYVVPVRTTREDSAGWVGA
CCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEE
IAHWNDDYTGLEMRVLRGETDFSWAESPRSRTRHFVSNIRTVAGPEADELTVRSNLLFFR
EEECCCCCCCEEEEEEECCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEE
SRGDSGRWELLSAERVDVLRRTDDSLRLARREVLLDHSTLPIDNLSVVL
ECCCCCCEEEECHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEC
>Mature Secondary Structure
MNAVAVDRDTREAVEAFMFREAELLDGGQFREWLGLLDPDIRYVVPVRTTREDSAGWVGA
CCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEE
IAHWNDDYTGLEMRVLRGETDFSWAESPRSRTRHFVSNIRTVAGPEADELTVRSNLLFFR
EEECCCCCCCEEEEEEECCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEE
SRGDSGRWELLSAERVDVLRRTDDSLRLARREVLLDHSTLPIDNLSVVL
ECCCCCCEEEECHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8890734 [H]