Click here to switch to the map view.

The map label for this gene is mii [H]

Identifier: 119776429

GI number: 119776429

Start: 3919355

End: 3920545

Strand: Direct

Name: mii [H]

Synonym: Sama_3297

Alternate gene names: 119776429

Gene position: 3919355-3920545 (Clockwise)

Preceding gene: 119776428

Following gene: 119776430

Centisome position: 91.02

GC content: 61.38

Gene sequence:

>1191_bases
ATGTTTAAACCACAGATCCGCATTCCTGCCACCTATATGCGTGGCGGAACCTCCAAAGGGGTGTTTTTCCGCCTCGACGA
TTTGCCTGAGGCGGCCCGGGTGCCGGGTGCAGAGCGTGATGCGCTGCTGCTGCGGGTGATTGGCAGCCCCGACCCCTATG
GCAAGCACACCGACGGCATGGGCGGCGCCACGTCCAGCACCAGCAAGTGCGTGATCATGAATAAAAGCAGCGTGCCCGGT
CACGATGTGGATTACCTCTACGGTCAGGTGTCCATCGACACCGCCTTCGTCGACTGGAGCGGTAACTGCGGCAACCTGTC
CACCGCCGCCGGCGCCTTTGCGATTCACGCGGGTTTGGTGGATGCTTCCCGGGTGCCTGAAAACGGTATCTGCGAAGTGC
GGATCTGGCAGGCCAATATCGGTAAAACCATTATTGCCCATGTGCCCGTGACCAACGGCGAAGTGCAGGAAACCGGTGAT
TTTGAACTGGATGGGGTGACCTTTCCGGCCGCCGAAATCGTGCTGGAGTTTGTCGATCCTGCGGATGATGGAGAGGATGG
TGGCGCCATGTTCCCCACCGGCAATCTGGTGGACGAGCTGGAAGTACCCTCCGAGGTGCACCCGAGCGGTAAAATCCGCG
CGACCCTGATAAACGCCGGTATCCCCACGATTTTCGTCAACGCCGAAGACCTGGGTTATCGCGGCGATGAGCTGCAGGAG
GCCATCAATGGCAATCCGGTGGCCCTTGCCATGTTTGAGCAGCTGCGTGCCCACGGCGCCATCAGGATGGGGCTTATCAA
GACCCTGGATGAAGCCAAAACCCGCCAGCACACCCCCAAGGTGGCCTTTGTGACCAACCCCATGACCCATGTGGTGTCGA
GCGGCAAGACAGTGGCTGAGGATGAAGTCGACCTGCTGGTCCGGGCGCTGTCCATGGGCAAGCTGCACCACGCCATGATG
GGCACAGCTGCGGTGGCCATAGGCACGGCGGCCGCCATTCCCGGCACATTGGTGAATCTTGCCGCCGGTGGCGGCGAGCG
TCAGGCGGTGCGTTTCGGCCATCCGTCCGGCACGTTACGCGTAGGCGCCGAGGCCAAACAGGTGGACGGCCAGTGGACTG
TCACCAAGGCCATCATGAGCCGCTCGGCGCGCATCCTGATGGAAGGCACTGTAAGGGTGCCTCAAGCTTAA

Upstream 100 bases:

>100_bases
CGAAAAAATGCGATTTTTCCTTGCCCGCAGGCACTGACATGTCTGGCGGCAGTTCCCTCTGCCGCTGAGGTTCCGCCCCA
ATCGTTTTTTGGAGATTAAA

Downstream 100 bases:

>100_bases
CCCACCTCTATGCCATATCCTTTTTGAGGGCTCCGCTTATTCCCTTCCGGCGGGGCCTTTTTTCTTTTTATCTTTTTGTG
TTACATGCAAATTGACACAC

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MFKPQIRIPATYMRGGTSKGVFFRLDDLPEAARVPGAERDALLLRVIGSPDPYGKHTDGMGGATSSTSKCVIMNKSSVPG
HDVDYLYGQVSIDTAFVDWSGNCGNLSTAAGAFAIHAGLVDASRVPENGICEVRIWQANIGKTIIAHVPVTNGEVQETGD
FELDGVTFPAAEIVLEFVDPADDGEDGGAMFPTGNLVDELEVPSEVHPSGKIRATLINAGIPTIFVNAEDLGYRGDELQE
AINGNPVALAMFEQLRAHGAIRMGLIKTLDEAKTRQHTPKVAFVTNPMTHVVSSGKTVAEDEVDLLVRALSMGKLHHAMM
GTAAVAIGTAAAIPGTLVNLAAGGGERQAVRFGHPSGTLRVGAEAKQVDGQWTVTKAIMSRSARILMEGTVRVPQA

Sequences:

>Translated_396_residues
MFKPQIRIPATYMRGGTSKGVFFRLDDLPEAARVPGAERDALLLRVIGSPDPYGKHTDGMGGATSSTSKCVIMNKSSVPG
HDVDYLYGQVSIDTAFVDWSGNCGNLSTAAGAFAIHAGLVDASRVPENGICEVRIWQANIGKTIIAHVPVTNGEVQETGD
FELDGVTFPAAEIVLEFVDPADDGEDGGAMFPTGNLVDELEVPSEVHPSGKIRATLINAGIPTIFVNAEDLGYRGDELQE
AINGNPVALAMFEQLRAHGAIRMGLIKTLDEAKTRQHTPKVAFVTNPMTHVVSSGKTVAEDEVDLLVRALSMGKLHHAMM
GTAAVAIGTAAAIPGTLVNLAAGGGERQAVRFGHPSGTLRVGAEAKQVDGQWTVTKAIMSRSARILMEGTVRVPQA
>Mature_396_residues
MFKPQIRIPATYMRGGTSKGVFFRLDDLPEAARVPGAERDALLLRVIGSPDPYGKHTDGMGGATSSTSKCVIMNKSSVPG
HDVDYLYGQVSIDTAFVDWSGNCGNLSTAAGAFAIHAGLVDASRVPENGICEVRIWQANIGKTIIAHVPVTNGEVQETGD
FELDGVTFPAAEIVLEFVDPADDGEDGGAMFPTGNLVDELEVPSEVHPSGKIRATLINAGIPTIFVNAEDLGYRGDELQE
AINGNPVALAMFEQLRAHGAIRMGLIKTLDEAKTRQHTPKVAFVTNPMTHVVSSGKTVAEDEVDLLVRALSMGKLHHAMM
GTAAVAIGTAAAIPGTLVNLAAGGGERQAVRFGHPSGTLRVGAEAKQVDGQWTVTKAIMSRSARILMEGTVRVPQA

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=388, Percent_Identity=31.4432989690722, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41787; Mature: 41787

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKPQIRIPATYMRGGTSKGVFFRLDDLPEAARVPGAERDALLLRVIGSPDPYGKHTDGM
CCCCCCCCCHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCC
GGATSSTSKCVIMNKSSVPGHDVDYLYGQVSIDTAFVDWSGNCGNLSTAAGAFAIHAGLV
CCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCHHHHHHEEEECCC
DASRVPENGICEVRIWQANIGKTIIAHVPVTNGEVQETGDFELDGVTFPAAEIVLEFVDP
CCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHHHHHHHCCC
ADDGEDGGAMFPTGNLVDELEVPSEVHPSGKIRATLINAGIPTIFVNAEDLGYRGDELQE
CCCCCCCCEECCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCEEEEECHHCCCCCHHHHH
AINGNPVALAMFEQLRAHGAIRMGLIKTLDEAKTRQHTPKVAFVTNPMTHVVSSGKTVAE
HHCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCEECH
DEVDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERQAVRFGHPSGTLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEE
VGAEAKQVDGQWTVTKAIMSRSARILMEGTVRVPQA
ECCCHHHCCCCHHHHHHHHHCCCEEEEECCEECCCC
>Mature Secondary Structure
MFKPQIRIPATYMRGGTSKGVFFRLDDLPEAARVPGAERDALLLRVIGSPDPYGKHTDGM
CCCCCCCCCHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCC
GGATSSTSKCVIMNKSSVPGHDVDYLYGQVSIDTAFVDWSGNCGNLSTAAGAFAIHAGLV
CCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCHHHHHHEEEECCC
DASRVPENGICEVRIWQANIGKTIIAHVPVTNGEVQETGDFELDGVTFPAAEIVLEFVDP
CCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHHHHHHHCCC
ADDGEDGGAMFPTGNLVDELEVPSEVHPSGKIRATLINAGIPTIFVNAEDLGYRGDELQE
CCCCCCCCEECCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCEEEEECHHCCCCCHHHHH
AINGNPVALAMFEQLRAHGAIRMGLIKTLDEAKTRQHTPKVAFVTNPMTHVVSSGKTVAE
HHCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCEECH
DEVDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERQAVRFGHPSGTLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEE
VGAEAKQVDGQWTVTKAIMSRSARILMEGTVRVPQA
ECCCHHHCCCCHHHHHHHHHCCCEEEEECCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA