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The map label for this gene is rpfG [H]

Identifier: 119776083

GI number: 119776083

Start: 3508091

End: 3509113

Strand: Reverse

Name: rpfG [H]

Synonym: Sama_2951

Alternate gene names: 119776083

Gene position: 3509113-3508091 (Counterclockwise)

Preceding gene: 119776084

Following gene: 119776080

Centisome position: 81.49

GC content: 56.99

Gene sequence:

>1023_bases
ATGGAAAAGGCCACAGTGCTGGTGGTGGATGATACCCCTGAGAACATCGATATTCTGGTGGGGATCTTGGGGCAGGATTA
TAAGGTCAAGGTTGCCATCGATGGCCCTCGGGCGCTGGCGCTGGCGCTGAAGTCGCCACCGGATCTGATTTTACTGGATG
TGATGATGCCGGGGATGAGTGGCTATGAGGTATGTCGCCGTCTCAAGCAGGAGCCCATGACGGCCCATATCCCGGTGATC
TTCGTTACCGCCCTTGCCGAGGTCGCCGACGAAACCCAGGGTTTTGAGTTGGGGGCCGTGGACTATATTACCAAGCCCGT
GAGCGCGCCCATTGTGAAGGTGAGGGTGAAAACCCACCTGGCACTTTACGACCAGAAAAGACTGCTCGAAGAGCAGGTGC
GGGAACGGACCAAAGAGCTCGAACAGACCCGTTTTGAGATTATCCGCCGTCTCGGCCGCGCCGCCGAATACAAGGACAAC
GAAACCGGGTTGCACGTGGTTCGCATGAGCCATTACGCAAGATTGCTGGCCCGACAGGCGGGGTTGCCTGAACCCTTCTG
TGAGCTCCTGTACAATGCCGCGCCCATGCATGATATCGGCAAAATAGGCACGCCCGATGCGGTGCTGAAAAAGCCCGCCA
AACTGGATGCCGAAGAGTGGGAAATCATGCAGCAGCACGCCAGCATAGGGGCCGAAATCATTGGCGAACATCCGGATCCA
CTGCTGCAGATGTCCCGCCGCATTGCTCTGACTCACCATGAAAAATGGGACGGTTCAGGCTATCCCAATGGTCTCAGCGG
CACTGAAATCCCCATCGAGGGGCGTATTGTGGCCATCGCCGATGTGTTCGATGCCCTGACCTCCATCAGGCCATACAAGA
AGGCATGGAGTATTGAAGACACCATGGCCTTGCTGGAGTCGGAAGCCGGCAAGCAGTTCGACCCCGAGCTGGTTGATCAC
TTCAAGCAGATATTGCCCGAGGTGGTTGCCGTGCGGGATAAGCACATGGAAACCGAAGGCTAG

Upstream 100 bases:

>100_bases
GGCGAATCCGCCTGACTATACTCAATAACAAATCGTTGTGGGCGCTGATTGCCCGGCTAACAAACTGCCCGCAGCAGGAG
CGGCAACAAGGGAGGTTGCG

Downstream 100 bases:

>100_bases
TCTGGCACTTGGCAGTGTTGCTTTTACCTGTCCCCGGATAGGAATAAAAAAGAGGCGCATCTGGCGCCTCTTTTTGTTAA
TCGGCGACAGGACTTACGCT

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MEKATVLVVDDTPENIDILVGILGQDYKVKVAIDGPRALALALKSPPDLILLDVMMPGMSGYEVCRRLKQEPMTAHIPVI
FVTALAEVADETQGFELGAVDYITKPVSAPIVKVRVKTHLALYDQKRLLEEQVRERTKELEQTRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLARQAGLPEPFCELLYNAAPMHDIGKIGTPDAVLKKPAKLDAEEWEIMQQHASIGAEIIGEHPDP
LLQMSRRIALTHHEKWDGSGYPNGLSGTEIPIEGRIVAIADVFDALTSIRPYKKAWSIEDTMALLESEAGKQFDPELVDH
FKQILPEVVAVRDKHMETEG

Sequences:

>Translated_340_residues
MEKATVLVVDDTPENIDILVGILGQDYKVKVAIDGPRALALALKSPPDLILLDVMMPGMSGYEVCRRLKQEPMTAHIPVI
FVTALAEVADETQGFELGAVDYITKPVSAPIVKVRVKTHLALYDQKRLLEEQVRERTKELEQTRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLARQAGLPEPFCELLYNAAPMHDIGKIGTPDAVLKKPAKLDAEEWEIMQQHASIGAEIIGEHPDP
LLQMSRRIALTHHEKWDGSGYPNGLSGTEIPIEGRIVAIADVFDALTSIRPYKKAWSIEDTMALLESEAGKQFDPELVDH
FKQILPEVVAVRDKHMETEG
>Mature_340_residues
MEKATVLVVDDTPENIDILVGILGQDYKVKVAIDGPRALALALKSPPDLILLDVMMPGMSGYEVCRRLKQEPMTAHIPVI
FVTALAEVADETQGFELGAVDYITKPVSAPIVKVRVKTHLALYDQKRLLEEQVRERTKELEQTRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLARQAGLPEPFCELLYNAAPMHDIGKIGTPDAVLKKPAKLDAEEWEIMQQHASIGAEIIGEHPDP
LLQMSRRIALTHHEKWDGSGYPNGLSGTEIPIEGRIVAIADVFDALTSIRPYKKAWSIEDTMALLESEAGKQFDPELVDH
FKQILPEVVAVRDKHMETEG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=142, Percent_Identity=36.6197183098592, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=40.495867768595, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=40.7079646017699, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693157, Length=122, Percent_Identity=37.7049180327869, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082012, Length=94, Percent_Identity=39.3617021276596, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=38.0952380952381, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=31.8965517241379, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 38005; Mature: 38005

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKATVLVVDDTPENIDILVGILGQDYKVKVAIDGPRALALALKSPPDLILLDVMMPGMS
CCCEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCCCEEEEEECCCCCC
GYEVCRRLKQEPMTAHIPVIFVTALAEVADETQGFELGAVDYITKPVSAPIVKVRVKTHL
HHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEEEHHHHH
ALYDQKRLLEEQVRERTKELEQTRFEIIRRLGRAAEYKDNETGLHVVRMSHYARLLARQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHC
GLPEPFCELLYNAAPMHDIGKIGTPDAVLKKPAKLDAEEWEIMQQHASIGAEIIGEHPDP
CCCHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCH
LLQMSRRIALTHHEKWDGSGYPNGLSGTEIPIEGRIVAIADVFDALTSIRPYKKAWSIED
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHCCHHH
TMALLESEAGKQFDPELVDHFKQILPEVVAVRDKHMETEG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKATVLVVDDTPENIDILVGILGQDYKVKVAIDGPRALALALKSPPDLILLDVMMPGMS
CCCEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCCCEEEEEECCCCCC
GYEVCRRLKQEPMTAHIPVIFVTALAEVADETQGFELGAVDYITKPVSAPIVKVRVKTHL
HHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEEEHHHHH
ALYDQKRLLEEQVRERTKELEQTRFEIIRRLGRAAEYKDNETGLHVVRMSHYARLLARQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHC
GLPEPFCELLYNAAPMHDIGKIGTPDAVLKKPAKLDAEEWEIMQQHASIGAEIIGEHPDP
CCCHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCH
LLQMSRRIALTHHEKWDGSGYPNGLSGTEIPIEGRIVAIADVFDALTSIRPYKKAWSIED
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHCCHHH
TMALLESEAGKQFDPELVDHFKQILPEVVAVRDKHMETEG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]