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The map label for this gene is braB [H]

Identifier: 119775872

GI number: 119775872

Start: 3273768

End: 3275093

Strand: Reverse

Name: braB [H]

Synonym: Sama_2740

Alternate gene names: 119775872

Gene position: 3275093-3273768 (Counterclockwise)

Preceding gene: 119775879

Following gene: 119775871

Centisome position: 76.06

GC content: 54.37

Gene sequence:

>1326_bases
GTGCAAAACAAGCAGATGAGTTTCTCAGACACCTTAAGTCTGGGCTTTATGACTTTTGCGTTCTTTTTGGGGGCCGGTAA
CCTGATATTCCCGCCATTCGCGGGCATGCTTGCCGGTGAGAACATGCCATTGGCCATGATGGGCTTTTTGGTGACTGCCG
TGGGCATGCCGTTGGTGGGTTTGATTGCTGTGGCCAAAACCCAGGGCAGGGTGATGGCGTATCTGCCTGCTGTGGCTGCT
ACGGCGTTGGCTGTGGCCATTTACATCATCATTGGCCCTGCCTTTGCGGCGCCCCGCACCGGTCTGGTGGCCTTTGAAAT
TGGCGCCAAACCCTTCCTCGGTGATACCAGTGCTGTATTGGCGCTTGGCAGTATCAGCCTCAATGTAGCTCAGCTTATCT
TTACCCTTGGGTTCTTTGCGGTGACCATGCTGCTGGCCCTCTTCCCCGGTAAATTGATGGACAGTGTGGGTAAAGTACTG
ACCCCTGTGCTCATGGTACTGCTGTTGTCGCTGGCCATTTCAGTAATCATGCTGCCATCGGGTGACACTTCAGCACCCGT
GGGGGATTATGCGTCCAGCCCGCTTATCAAGGGGGTGATTGAAGGCTACAACACCATGGATACTCTGGCGTCGCTGATGT
TCGGTATGCTGATTATCGACCTTTTACGCAAGAAAGGGGTTGATGCCCCTGCGGACCAAACCCGTGCACTGGTGCGTGCG
GCCTTTATCGCCGCATCCGGTTTGGCCTTTGTGTATATTTCGCTGTTTTTCCTCGGAGCCACTGCAGGGGATATCGCTCA
GGGTGCCGAAAATGGTGGTCAAATTCTGACCCGTTATGTCGACCGTGAGTTTGGGACACTGGGTGCGCTGCTGTTGTCTA
CCGTGGTGTCTCTGGCATGTTTGACCACGGCTGTGGGTTTGGTGAGTGCCTGTTCTGATTATTTCAATGAGCTGTTTCCA
CGTATTAAATACCGTCAGTTCGTTATCCTGTTTTCCATCATCTGCGCCGTAGTGGCCAACGTCGGGCTGACTCAGCTTAT
CAGCGTAAGTATTCCTGTGCTGGTGAGTGTTTATCCCGTGGTGATAGCCTTGGTGGCCGTGACCTTTATGACCCAGTACC
TGCCCAACCCCAAAGTCAGCCATCGACTGGTGTTGGCGGTGGCGCTGCTTTTTGGTGCCATCGATGGCCTCAAGGCTGCC
GGGGTGAATATGGAGTCTCTGAGCTTTATGCCGCTTTATGCCGAAGGCATGGCCTGGTTGTTGCCAACCGCAGTGACCAT
GGTAATCTGCCTCTTTATCGGTAAGCGCGCACCGGCTGCGGCCTGA

Upstream 100 bases:

>100_bases
TGGCGTTACACGGGGCTTTCTCTGTCCGTCTTCGGTAGCCAAGTTGCAGATAATCACTTAAGTTTGTTCGAAAAACACTA
TAAATAAGATGGGGCTTCTT

Downstream 100 bases:

>100_bases
TAGCAATATGCAAGTAAAAGCCGGGTTCGCCCGGCTTTTTTGTTTTTCCGTTGGAGTCTTTTTTGTCCGCCAAAGCCAAT
ACCTCCCGTAAGGGTGGCAA

Product: branched-chain amino acid transport system II carrier protein BrnQ

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MQNKQMSFSDTLSLGFMTFAFFLGAGNLIFPPFAGMLAGENMPLAMMGFLVTAVGMPLVGLIAVAKTQGRVMAYLPAVAA
TALAVAIYIIIGPAFAAPRTGLVAFEIGAKPFLGDTSAVLALGSISLNVAQLIFTLGFFAVTMLLALFPGKLMDSVGKVL
TPVLMVLLLSLAISVIMLPSGDTSAPVGDYASSPLIKGVIEGYNTMDTLASLMFGMLIIDLLRKKGVDAPADQTRALVRA
AFIAASGLAFVYISLFFLGATAGDIAQGAENGGQILTRYVDREFGTLGALLLSTVVSLACLTTAVGLVSACSDYFNELFP
RIKYRQFVILFSIICAVVANVGLTQLISVSIPVLVSVYPVVIALVAVTFMTQYLPNPKVSHRLVLAVALLFGAIDGLKAA
GVNMESLSFMPLYAEGMAWLLPTAVTMVICLFIGKRAPAAA

Sequences:

>Translated_441_residues
MQNKQMSFSDTLSLGFMTFAFFLGAGNLIFPPFAGMLAGENMPLAMMGFLVTAVGMPLVGLIAVAKTQGRVMAYLPAVAA
TALAVAIYIIIGPAFAAPRTGLVAFEIGAKPFLGDTSAVLALGSISLNVAQLIFTLGFFAVTMLLALFPGKLMDSVGKVL
TPVLMVLLLSLAISVIMLPSGDTSAPVGDYASSPLIKGVIEGYNTMDTLASLMFGMLIIDLLRKKGVDAPADQTRALVRA
AFIAASGLAFVYISLFFLGATAGDIAQGAENGGQILTRYVDREFGTLGALLLSTVVSLACLTTAVGLVSACSDYFNELFP
RIKYRQFVILFSIICAVVANVGLTQLISVSIPVLVSVYPVVIALVAVTFMTQYLPNPKVSHRLVLAVALLFGAIDGLKAA
GVNMESLSFMPLYAEGMAWLLPTAVTMVICLFIGKRAPAAA
>Mature_441_residues
MQNKQMSFSDTLSLGFMTFAFFLGAGNLIFPPFAGMLAGENMPLAMMGFLVTAVGMPLVGLIAVAKTQGRVMAYLPAVAA
TALAVAIYIIIGPAFAAPRTGLVAFEIGAKPFLGDTSAVLALGSISLNVAQLIFTLGFFAVTMLLALFPGKLMDSVGKVL
TPVLMVLLLSLAISVIMLPSGDTSAPVGDYASSPLIKGVIEGYNTMDTLASLMFGMLIIDLLRKKGVDAPADQTRALVRA
AFIAASGLAFVYISLFFLGATAGDIAQGAENGGQILTRYVDREFGTLGALLLSTVVSLACLTTAVGLVSACSDYFNELFP
RIKYRQFVILFSIICAVVANVGLTQLISVSIPVLVSVYPVVIALVAVTFMTQYLPNPKVSHRLVLAVALLFGAIDGLKAA
GVNMESLSFMPLYAEGMAWLLPTAVTMVICLFIGKRAPAAA

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=444, Percent_Identity=39.4144144144144, Blast_Score=308, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46185; Mature: 46185

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKQMSFSDTLSLGFMTFAFFLGAGNLIFPPFAGMLAGENMPLAMMGFLVTAVGMPLVG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LIAVAKTQGRVMAYLPAVAATALAVAIYIIIGPAFAAPRTGLVAFEIGAKPFLGDTSAVL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEECCCCCCCCHHHHH
ALGSISLNVAQLIFTLGFFAVTMLLALFPGKLMDSVGKVLTPVLMVLLLSLAISVIMLPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GDTSAPVGDYASSPLIKGVIEGYNTMDTLASLMFGMLIIDLLRKKGVDAPADQTRALVRA
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AFIAASGLAFVYISLFFLGATAGDIAQGAENGGQILTRYVDREFGTLGALLLSTVVSLAC
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTAVGLVSACSDYFNELFPRIKYRQFVILFSIICAVVANVGLTQLISVSIPVLVSVYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VIALVAVTFMTQYLPNPKVSHRLVLAVALLFGAIDGLKAAGVNMESLSFMPLYAEGMAWL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHCCCCHHHCCCCHHHHHHHHHH
LPTAVTMVICLFIGKRAPAAA
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQNKQMSFSDTLSLGFMTFAFFLGAGNLIFPPFAGMLAGENMPLAMMGFLVTAVGMPLVG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LIAVAKTQGRVMAYLPAVAATALAVAIYIIIGPAFAAPRTGLVAFEIGAKPFLGDTSAVL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEECCCCCCCCHHHHH
ALGSISLNVAQLIFTLGFFAVTMLLALFPGKLMDSVGKVLTPVLMVLLLSLAISVIMLPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GDTSAPVGDYASSPLIKGVIEGYNTMDTLASLMFGMLIIDLLRKKGVDAPADQTRALVRA
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AFIAASGLAFVYISLFFLGATAGDIAQGAENGGQILTRYVDREFGTLGALLLSTVVSLAC
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTAVGLVSACSDYFNELFPRIKYRQFVILFSIICAVVANVGLTQLISVSIPVLVSVYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VIALVAVTFMTQYLPNPKVSHRLVLAVALLFGAIDGLKAAGVNMESLSFMPLYAEGMAWL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHCCCCHHHCCCCHHHHHHHHHH
LPTAVTMVICLFIGKRAPAAA
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]