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The map label for this gene is potF [H]

Identifier: 119775774

GI number: 119775774

Start: 3165155

End: 3166255

Strand: Reverse

Name: potF [H]

Synonym: Sama_2642

Alternate gene names: 119775774

Gene position: 3166255-3165155 (Counterclockwise)

Preceding gene: 119775775

Following gene: 119775773

Centisome position: 73.53

GC content: 52.95

Gene sequence:

>1101_bases
ATGAAGTTATTACACAAAATGACAGCTCTGGCTTTGGTTACCGCCGGTGTTCTGGGAAGCGCTGCGGTATCGGCTGAAGA
AGTTGTTCGCGTCTATAACTGGTCTGACTATATCGCCGAAGACACCCTGGAAAACTTCCAGAAGGAAACCGGCATCCGCG
TAATCTACGATGTGTTCGACACCAACGAAGTGCTGGAAGCCAAGCTGCTTTCAGGCCGCAGCGGTTACGATCTGGTTGTG
CCATCAGCCCACTTCCTTGCCAAGCAGATTAAAGCCGGCGCCTTTATGAAGCTGGACAAGTCCAAGCTGACAAACGAAAA
GCACCTCAACAAGAATCTGATGACCCAGCTGCAAAAGAGCGATCCGGGTAACGAGCACGCCGTGCCTTACCTGTGGGGCA
CCACCGGCATCGGCTTCAACGTGGCCAAGGTAAAAGAAGTGCTGGGTGAAGATGCACCGGTGGATTCCTGGGACTTGATT
TTCAACCCAGCCAATGCTGAAAAGCTGGCCAAGTGCGGTATTGCCGTGCTGGATTCACCCGATGAAATCCTGCCACCTGC
GCTGAACTATCTGGGCCTGAACCCTAACAGCACTGATCCTGCCGACTATGAAAAAGCCGGTGAAGTACTGCTGAAAATCC
GTCCTTACCTCAAGTATTTCCACTCTTCACGCTACATCTCTGACTTGGCCAATGGCGAAATCTGTGCCGCCATCGGTTGG
TCCGGTGACGTACTGCAGGCTCAGGCCCGTGCCGAAGAGGCCGGTAACGGCCAGCAAGTGGATTATCGCATCCCTAAAGA
AGGTGCACCTTTGTGGTTTGACATGATGGCTATCCCTGCCGATGCTGCCAACGCTGACAATGCGCTGACCTTTATGAATT
ACCTGATGCGCCCAGAGGTCATCGCTTCCATCAGTAACTATGTGGCTTATGCCAACGCCAACGATGGTTCTAACCCTCTG
TTGGACGAGGAAGTTGCCAAAAACCCCGCTGTGTATCCATCAGAAGAAACACTCGCCAAGCTCTACCTGGGTGAAGTGCG
TCCTATGAAGACCCAGCGCGCCATGACCCGGATCTGGACCAAGATCAAATCTGGTCAGTGA

Upstream 100 bases:

>100_bases
TTGTAGCATTGGTCCTAACAATAAAATAAATGCCGGCCAAGCCGGCGACACAATCAGGGTAATTGGCTTTCAATTACTGC
TACCTGCAAAAGGAGATAGC

Downstream 100 bases:

>100_bases
TCCTGTACTTGGGGCAGCGGATGGCTGCCCCTCCTTTGACTCTTAGATTGCACAAGTAAAACGAATTCCAGCAGGCCGGA
GGCTTGTTCTGGCGCAGTTT

Product: polyamine ABC transporter periplasmic polyamine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKLLHKMTALALVTAGVLGSAAVSAEEVVRVYNWSDYIAEDTLENFQKETGIRVIYDVFDTNEVLEAKLLSGRSGYDLVV
PSAHFLAKQIKAGAFMKLDKSKLTNEKHLNKNLMTQLQKSDPGNEHAVPYLWGTTGIGFNVAKVKEVLGEDAPVDSWDLI
FNPANAEKLAKCGIAVLDSPDEILPPALNYLGLNPNSTDPADYEKAGEVLLKIRPYLKYFHSSRYISDLANGEICAAIGW
SGDVLQAQARAEEAGNGQQVDYRIPKEGAPLWFDMMAIPADAANADNALTFMNYLMRPEVIASISNYVAYANANDGSNPL
LDEEVAKNPAVYPSEETLAKLYLGEVRPMKTQRAMTRIWTKIKSGQ

Sequences:

>Translated_366_residues
MKLLHKMTALALVTAGVLGSAAVSAEEVVRVYNWSDYIAEDTLENFQKETGIRVIYDVFDTNEVLEAKLLSGRSGYDLVV
PSAHFLAKQIKAGAFMKLDKSKLTNEKHLNKNLMTQLQKSDPGNEHAVPYLWGTTGIGFNVAKVKEVLGEDAPVDSWDLI
FNPANAEKLAKCGIAVLDSPDEILPPALNYLGLNPNSTDPADYEKAGEVLLKIRPYLKYFHSSRYISDLANGEICAAIGW
SGDVLQAQARAEEAGNGQQVDYRIPKEGAPLWFDMMAIPADAANADNALTFMNYLMRPEVIASISNYVAYANANDGSNPL
LDEEVAKNPAVYPSEETLAKLYLGEVRPMKTQRAMTRIWTKIKSGQ
>Mature_366_residues
MKLLHKMTALALVTAGVLGSAAVSAEEVVRVYNWSDYIAEDTLENFQKETGIRVIYDVFDTNEVLEAKLLSGRSGYDLVV
PSAHFLAKQIKAGAFMKLDKSKLTNEKHLNKNLMTQLQKSDPGNEHAVPYLWGTTGIGFNVAKVKEVLGEDAPVDSWDLI
FNPANAEKLAKCGIAVLDSPDEILPPALNYLGLNPNSTDPADYEKAGEVLLKIRPYLKYFHSSRYISDLANGEICAAIGW
SGDVLQAQARAEEAGNGQQVDYRIPKEGAPLWFDMMAIPADAANADNALTFMNYLMRPEVIASISNYVAYANANDGSNPL
LDEEVAKNPAVYPSEETLAKLYLGEVRPMKTQRAMTRIWTKIKSGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=359, Percent_Identity=56.2674094707521, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1787367, Length=339, Percent_Identity=34.2182890855457, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787711, Length=314, Percent_Identity=24.8407643312102, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40221; Mature: 40221

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLHKMTALALVTAGVLGSAAVSAEEVVRVYNWSDYIAEDTLENFQKETGIRVIYDVFD
CHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
TNEVLEAKLLSGRSGYDLVVPSAHFLAKQIKAGAFMKLDKSKLTNEKHLNKNLMTQLQKS
CHHHHHHHHHCCCCCCEEECCCHHHHHHHHCCCCCEEECHHHCCCHHHHCHHHHHHHHHC
DPGNEHAVPYLWGTTGIGFNVAKVKEVLGEDAPVDSWDLIFNPANAEKLAKCGIAVLDSP
CCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEEEECCC
DEILPPALNYLGLNPNSTDPADYEKAGEVLLKIRPYLKYFHSSRYISDLANGEICAAIGW
HHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
SGDVLQAQARAEEAGNGQQVDYRIPKEGAPLWFDMMAIPADAANADNALTFMNYLMRPEV
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEHEECCCCCCCCCHHHHHHHHHHCHHH
IASISNYVAYANANDGSNPLLDEEVAKNPAVYPSEETLAKLYLGEVRPMKTQRAMTRIWT
HHHHHHHEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
KIKSGQ
HHCCCC
>Mature Secondary Structure
MKLLHKMTALALVTAGVLGSAAVSAEEVVRVYNWSDYIAEDTLENFQKETGIRVIYDVFD
CHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
TNEVLEAKLLSGRSGYDLVVPSAHFLAKQIKAGAFMKLDKSKLTNEKHLNKNLMTQLQKS
CHHHHHHHHHCCCCCCEEECCCHHHHHHHHCCCCCEEECHHHCCCHHHHCHHHHHHHHHC
DPGNEHAVPYLWGTTGIGFNVAKVKEVLGEDAPVDSWDLIFNPANAEKLAKCGIAVLDSP
CCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEEEECCC
DEILPPALNYLGLNPNSTDPADYEKAGEVLLKIRPYLKYFHSSRYISDLANGEICAAIGW
HHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
SGDVLQAQARAEEAGNGQQVDYRIPKEGAPLWFDMMAIPADAANADNALTFMNYLMRPEV
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEHEECCCCCCCCCHHHHHHHHHHCHHH
IASISNYVAYANANDGSNPLLDEEVAKNPAVYPSEETLAKLYLGEVRPMKTQRAMTRIWT
HHHHHHHEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
KIKSGQ
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]