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The map label for this gene is bioB

Identifier: 119774575

GI number: 119774575

Start: 1748465

End: 1749529

Strand: Direct

Name: bioB

Synonym: Sama_1438

Alternate gene names: 119774575

Gene position: 1748465-1749529 (Clockwise)

Preceding gene: 119774573

Following gene: 119774576

Centisome position: 40.6

GC content: 55.87

Gene sequence:

>1065_bases
ATGTCATCCTTTGCCGTTCGTCACGACTGGAGCCGTCAGGAAGTCGAGGCGCTGTTTGCACTGCCAATGAACGATTTGCT
GTTTCGTGCCCACAGCATACACCGCGAAGTGTTCGATCCCAATGAGGTACAGATAAGCCGTTTGCTATCGATTAAAACCG
GTGCTTGCCCCGAGGACTGTAAATACTGCCCTCAAAGCGCCCGTTACGATACCGGTCTTGAAAAAGAGCGCCTGCTGGAA
ATTGAGAAGGTGCTGACCGAAGCCCGCGCCGCCAAAGACGCCGGTGCCACCCGTTTTTGTATGGGGGCCGCCTGGCGTAA
TCCCCATGAGCGTGATATGCCGTATCTGACTGATATGGTCAAAGAAGTAAAGTCCATGGGGCTGGAAACCTGCATGACAC
TGGGCATGCTTTCGGCCCATCAGGCCAATCAGCTTGCCGAAGCCGGTCTGGATTATTACAACCACAACCTGGATACGTCT
CCGGAGTTTTACGGCGATATCATCACCACCCGCACCTATCAGGACAGGCTCGATACCCTTTCCAATGTGCGTGCCGCCGG
TATGAAGGTGTGTTCTGGTGGTATCGTCGGCATGGGCGAGCAGGCCACCGACCGTGCCGGTTTGCTGCAGCAATTGGCTA
ACCTAGAACAACATCCGGACTCAGTGCCCATCAATATGTTGGTGAAGGTGACCGGCACGCCACTGGACAGCGTGGATGAT
TTGGATCCGCTGGAATTTGTGCGCACCATCGCCGTTGCCCGTATTTTGATGCCGCTCTCAAGGGTACGTTTGTCTGCCGG
TCGCGAGAACATGAGTGACGAGCTGCAGGCCATGTGTTTCTTTGCCGGTGCCAACTCGATTTTTTACGGCTGCAAGTTGC
TGACCACACCAAATCCTGAAGAAAACGATGATATGAGCCTGTTCCGCCGCTTGGGCCTCAAACCCGAGCAAGGCAAGGCG
GCCATCGTCGAGGAAGATGCCGCCGTGGTGGCGCGGGCAGCCAGGGAGCAACAGGCTGAGGGCGAAGCCAAGACCAAACC
GCTTTTCTACGACGCGGCCAACTGA

Upstream 100 bases:

>100_bases
CAAAATTTAATCATCAGTAAACCCTGCCAACTGGTTGGCGTTGACAGTGTAAGGGCCAGGGTTATCCTAGCCAACAGAAA
ACATTAATTGGGGTAATACC

Downstream 100 bases:

>100_bases
GCCGGATTTACCAGTGATGAATCGGTTGCTGCATCGCCTGGCTGAGGCCAGAGTGAAAGCCGAATCGGCGGGGCTTTGGC
GTCAACGTCAGCGCCACAGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSSFAVRHDWSRQEVEALFALPMNDLLFRAHSIHREVFDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLE
IEKVLTEARAAKDAGATRFCMGAAWRNPHERDMPYLTDMVKEVKSMGLETCMTLGMLSAHQANQLAEAGLDYYNHNLDTS
PEFYGDIITTRTYQDRLDTLSNVRAAGMKVCSGGIVGMGEQATDRAGLLQQLANLEQHPDSVPINMLVKVTGTPLDSVDD
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEENDDMSLFRRLGLKPEQGKA
AIVEEDAAVVARAAREQQAEGEAKTKPLFYDAAN

Sequences:

>Translated_354_residues
MSSFAVRHDWSRQEVEALFALPMNDLLFRAHSIHREVFDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLE
IEKVLTEARAAKDAGATRFCMGAAWRNPHERDMPYLTDMVKEVKSMGLETCMTLGMLSAHQANQLAEAGLDYYNHNLDTS
PEFYGDIITTRTYQDRLDTLSNVRAAGMKVCSGGIVGMGEQATDRAGLLQQLANLEQHPDSVPINMLVKVTGTPLDSVDD
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEENDDMSLFRRLGLKPEQGKA
AIVEEDAAVVARAAREQQAEGEAKTKPLFYDAAN
>Mature_353_residues
SSFAVRHDWSRQEVEALFALPMNDLLFRAHSIHREVFDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLEI
EKVLTEARAAKDAGATRFCMGAAWRNPHERDMPYLTDMVKEVKSMGLETCMTLGMLSAHQANQLAEAGLDYYNHNLDTSP
EFYGDIITTRTYQDRLDTLSNVRAAGMKVCSGGIVGMGEQATDRAGLLQQLANLEQHPDSVPINMLVKVTGTPLDSVDDL
DPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEENDDMSLFRRLGLKPEQGKAA
IVEEDAAVVARAAREQQAEGEAKTKPLFYDAAN

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=353, Percent_Identity=66.8555240793201, Blast_Score=486, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=46.8553459119497, Blast_Score=313, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_SHEAM (A1S5I9)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927315.1
- ProteinModelPortal:   A1S5I9
- SMR:   A1S5I9
- STRING:   A1S5I9
- GeneID:   4603690
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1438
- NMPDR:   fig|326297.7.peg.1356
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   A1S5I9
- ProtClustDB:   CLSK2304484
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39317; Mature: 39186

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFAVRHDWSRQEVEALFALPMNDLLFRAHSIHREVFDPNEVQISRLLSIKTGACPEDC
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLEIEKVLTEARAAKDAGATRFCMGAAWRNPHERDMPYLTDMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
KEVKSMGLETCMTLGMLSAHQANQLAEAGLDYYNHNLDTSPEFYGDIITTRTYQDRLDTL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHH
SNVRAAGMKVCSGGIVGMGEQATDRAGLLQQLANLEQHPDSVPINMLVKVTGTPLDSVDD
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ENDDMSLFRRLGLKPEQGKAAIVEEDAAVVARAAREQQAEGEAKTKPLFYDAAN
CCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
>Mature Secondary Structure 
SSFAVRHDWSRQEVEALFALPMNDLLFRAHSIHREVFDPNEVQISRLLSIKTGACPEDC
CCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLEIEKVLTEARAAKDAGATRFCMGAAWRNPHERDMPYLTDMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
KEVKSMGLETCMTLGMLSAHQANQLAEAGLDYYNHNLDTSPEFYGDIITTRTYQDRLDTL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHH
SNVRAAGMKVCSGGIVGMGEQATDRAGLLQQLANLEQHPDSVPINMLVKVTGTPLDSVDD
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ENDDMSLFRRLGLKPEQGKAAIVEEDAAVVARAAREQQAEGEAKTKPLFYDAAN
CCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA