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The map label for this gene is int [H]

Identifier: 119774492

GI number: 119774492

Start: 1643758

End: 1644714

Strand: Direct

Name: int [H]

Synonym: Sama_1355

Alternate gene names: 119774492

Gene position: 1643758-1644714 (Clockwise)

Preceding gene: 119774477

Following gene: 119774501

Centisome position: 38.17

GC content: 48.59

Gene sequence:

>957_bases
ATGACACACAGTCCCTTCCTGAATGCTGTCCGCGACAGTATACGTTTGAGAGGCTACAGCTTGCGCACGGAGAAAACTTA
CCTTCATTGGATTAAGTTTTACATTCTTTTCAATCAAAAACGCCATCCTGACACTATGGGCAGCGCGGAAATTACCGCGT
TTTTAACCTATCTTGCTGTAGAGCGCCATGTGGCCGCGAATACCCAAAAAGTGGCCTTAAATGCGTTGATCTATCTGTAT
CAAAAGTTTTTACAGATTGAGCTTGGCGAGTTGCCTTTTTCATTGGCCACCAAGCAGCGTTCTTTGCCCATCGTCGTCAC
ACAGACAGAGGTCGCACATATCCTCGCTAAACTGAGTGGGGTTCACTTACATGTGATTCAAATGCTCTACGGCAGTGGGC
TCAGAGTGACAGAATGTCTCAGGCTTAGGGTGCAGGATGTCGACTTGGAGCGGTTATCTGTGACCGTGCATGACGGTAAA
GGGAATAAGGACCGCCAAACGTTGCTCAGCCGAAGTTGCCTGAATTGGTTACCGGATCAAATTGAACATAGTCTGAATAT
CCAGCAAAAAGACAATCAACGTGGTATTGGACCATCGATGCCTACAGCACTTTCGGTAAAGTACCCGAATGCCTTTCGCG
AACCTGGCTGGATGTATTTGTTTCCGAGTAGTGGATTATGCCCTCATCCTATCACCGGTGAAATTTGCCGCCATCATCTT
CACGACAGTGCGATTCGCAAAGCGTTGAGAATCGCGGTGCGTCATGCTGGGGTGCGCAAAAAGGTGAATTGCCATACTTT
CCGTCATTCCTTCGCTACTCACTTGCTGCAGGCCGGATACGATATCCGTACTGTGCAGGAGTTATTGGGCCATAACGATG
TAAAGACAACCCAGATTTACACCCACGTACTGGGCCAGCATTACGCCGGAACAGTCAGCCCCCTTGATCGGTTGTGA

Upstream 100 bases:

>100_bases
GCTGGGTGATTCGTGCCAATCAATTGAGATAACCGTGCTTGCATTTTGAAAAACACCATTAATACTGTTTTTATATACAG
TTGTTTTTTTGAGGTGTTTG

Downstream 100 bases:

>100_bases
CTATGCCATCCCGACTCACCATCCCCGATTCACTATTCCCAATTCCCGATTCACCATCCCTGATTCACCATTGTGGCTAC
CGGTAAACGCCATGGCTACC

Product: phage integrase family site specific recombinase

Products: NA

Alternate protein names: E2 protein [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTHSPFLNAVRDSIRLRGYSLRTEKTYLHWIKFYILFNQKRHPDTMGSAEITAFLTYLAVERHVAANTQKVALNALIYLY
QKFLQIELGELPFSLATKQRSLPIVVTQTEVAHILAKLSGVHLHVIQMLYGSGLRVTECLRLRVQDVDLERLSVTVHDGK
GNKDRQTLLSRSCLNWLPDQIEHSLNIQQKDNQRGIGPSMPTALSVKYPNAFREPGWMYLFPSSGLCPHPITGEICRHHL
HDSAIRKALRIAVRHAGVRKKVNCHTFRHSFATHLLQAGYDIRTVQELLGHNDVKTTQIYTHVLGQHYAGTVSPLDRL

Sequences:

>Translated_318_residues
MTHSPFLNAVRDSIRLRGYSLRTEKTYLHWIKFYILFNQKRHPDTMGSAEITAFLTYLAVERHVAANTQKVALNALIYLY
QKFLQIELGELPFSLATKQRSLPIVVTQTEVAHILAKLSGVHLHVIQMLYGSGLRVTECLRLRVQDVDLERLSVTVHDGK
GNKDRQTLLSRSCLNWLPDQIEHSLNIQQKDNQRGIGPSMPTALSVKYPNAFREPGWMYLFPSSGLCPHPITGEICRHHL
HDSAIRKALRIAVRHAGVRKKVNCHTFRHSFATHLLQAGYDIRTVQELLGHNDVKTTQIYTHVLGQHYAGTVSPLDRL
>Mature_317_residues
THSPFLNAVRDSIRLRGYSLRTEKTYLHWIKFYILFNQKRHPDTMGSAEITAFLTYLAVERHVAANTQKVALNALIYLYQ
KFLQIELGELPFSLATKQRSLPIVVTQTEVAHILAKLSGVHLHVIQMLYGSGLRVTECLRLRVQDVDLERLSVTVHDGKG
NKDRQTLLSRSCLNWLPDQIEHSLNIQQKDNQRGIGPSMPTALSVKYPNAFREPGWMYLFPSSGLCPHPITGEICRHHLH
DSAIRKALRIAVRHAGVRKKVNCHTFRHSFATHLLQAGYDIRTVQELLGHNDVKTTQIYTHVLGQHYAGTVSPLDRL

Specific function: Putative integrase believed to be involved in the insertion of antibiotic resistance genes into plasmids and transposons [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1789261, Length=180, Percent_Identity=34.4444444444444, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790244, Length=185, Percent_Identity=32.972972972973, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR011946
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 36247; Mature: 36116

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSPFLNAVRDSIRLRGYSLRTEKTYLHWIKFYILFNQKRHPDTMGSAEITAFLTYLAV
CCCCHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ERHVAANTQKVALNALIYLYQKFLQIELGELPFSLATKQRSLPIVVTQTEVAHILAKLSG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEHHHHHHHHHHHCC
VHLHVIQMLYGSGLRVTECLRLRVQDVDLERLSVTVHDGKGNKDRQTLLSRSCLNWLPDQ
HHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHH
IEHSLNIQQKDNQRGIGPSMPTALSVKYPNAFREPGWMYLFPSSGLCPHPITGEICRHHL
HHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHH
HDSAIRKALRIAVRHAGVRKKVNCHTFRHSFATHLLQAGYDIRTVQELLGHNDVKTTQIY
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
THVLGQHYAGTVSPLDRL
HHHHHHHCCCCCCHHHCC
>Mature Secondary Structure 
THSPFLNAVRDSIRLRGYSLRTEKTYLHWIKFYILFNQKRHPDTMGSAEITAFLTYLAV
CCCHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ERHVAANTQKVALNALIYLYQKFLQIELGELPFSLATKQRSLPIVVTQTEVAHILAKLSG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEHHHHHHHHHHHCC
VHLHVIQMLYGSGLRVTECLRLRVQDVDLERLSVTVHDGKGNKDRQTLLSRSCLNWLPDQ
HHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHH
IEHSLNIQQKDNQRGIGPSMPTALSVKYPNAFREPGWMYLFPSSGLCPHPITGEICRHHL
HHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHH
HDSAIRKALRIAVRHAGVRKKVNCHTFRHSFATHLLQAGYDIRTVQELLGHNDVKTTQIY
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
THVLGQHYAGTVSPLDRL
HHHHHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2821509; 2823258; 3054482; 8195081; 2188588 [H]