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The map label for this gene is iscS

Identifier: 119774430

GI number: 119774430

Start: 1579903

End: 1581117

Strand: Direct

Name: iscS

Synonym: Sama_1293

Alternate gene names: 119774430

Gene position: 1579903-1581117 (Clockwise)

Preceding gene: 119774429

Following gene: 119774431

Centisome position: 36.69

GC content: 53.42

Gene sequence:

>1215_bases
ATGAAGCTTCCTATCTATTTCGATTACGCCGCAACGACCCCGGTTGACCCGCGCGTTGCTCAAAAAATGATGCAGTACAT
GACGATGGACGGCATCTTTGGTAACCCGGCTTCCCGTTCCCATCGTTACGGTTGGCAGGCGGAAGAAGCGGTAGATGTCG
CCCGTTCGCAGGTGGCAGATCTGATTAATGCCGATCACCGCGAAATCGTGTTTACGTCCGGCGCCACCGAGTCAAACAAC
CTGGCTATTAAAGGCGTTGCACACTTCTATCAGAAGAAAGGCAAACACATCATCACGAGCAAAACCGAGCACAAAGCCGT
GCTGGATACGTGCCGCCAGCTGGAACGTGAAGGTTTTGAAGTGACTTACCTCGAGCCTGGTGTGAACGGCATCATTCCCA
TGGAGCGCCTTGAGGCAGCCATGCGTGACGACACCATTCTGCTGAGCCTGATGCACGTGAATAACGAAATCGGCGTGGTA
CATGATATCGATGCCATTGGTGAGCTGTGCCGCAGCAAGGGCATTATTTTCCACGTGGATGCTGCCCAGAGCGCCGGTAA
ACTGCCGATTGACGTGCAAATTACCAAGGTTGACCTGATGTCGATTTCCGGTCACAAGATGTATGGCCCTAAAGGTATTG
GTGCGCTGTATGTGCGCCGTAAGCCACGCATCCGTCTGGAAAGCCAGATGCACGGTGGCGGACACGAGCGCGGCATGCGC
AGCGGCACTCTGGCTACGCATCAGATAGTAGGCCTCGGTGAAGCGGCTGCCATTGCCAAAGCAGATATGGAAAGCGATAA
CGCCCGTATCCACGCTCTGCGTGACCGCCTGTGGAACGGTATCAAAGATATCGAAGAGACCTATGTTAACGGTGACTTCG
AGCAGCGCTACTGCGGTAACCTGAACGTCAGCTTTAACTTTGTTGAAGGTGAGTCCTTGATGATGGCCCTGAAAGATTTA
GCCGTATCATCGGGCTCTGCCTGTACGTCTGCCAGTCTTGAGCCAAGCTATGTGCTGCGCGCTTTGGGACTGAGCGATGA
AATGGCCCACAGCTCAATCCGTTTTTCCATCGGTCGCTTTACCACCGAGGAAGAAGTGGATTACGCCATCGGCATTATCC
AGGGCTCTATCGGCAAGCTCAGAGAAATGTCCCCACTTTGGGAGATGTTCAAAGATGGTGTCGATTTGAGCAAGGTTGAG
TGGGTACACCACTGA

Upstream 100 bases:

>100_bases
AAGGGCACAGGGCATCGGCTTAAGTCCTTGTGAATCCATAATAATTAAGCATGTACGTTGTAAGTAGCCTCGGTTGAGAC
TGCGAAGTACGGAGTGTGTG

Downstream 100 bases:

>100_bases
TTGGTGGGTTGAGAGATTTGGAGCAGTATCATGGCATACAGTGAAAAAGTGATAGATCATTACGAGAATCCCCGTAACGT
GGGTTCTTTTGACAAGAACG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYFDYAATTPVDPRVAQKMMQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVADLINADHREIVFTSGATESNN
LAIKGVAHFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPGVNGIIPMERLEAAMRDDTILLSLMHVNNEIGVV
HDIDAIGELCRSKGIIFHVDAAQSAGKLPIDVQITKVDLMSISGHKMYGPKGIGALYVRRKPRIRLESQMHGGGHERGMR
SGTLATHQIVGLGEAAAIAKADMESDNARIHALRDRLWNGIKDIEETYVNGDFEQRYCGNLNVSFNFVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLSDEMAHSSIRFSIGRFTTEEEVDYAIGIIQGSIGKLREMSPLWEMFKDGVDLSKVE
WVHH

Sequences:

>Translated_404_residues
MKLPIYFDYAATTPVDPRVAQKMMQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVADLINADHREIVFTSGATESNN
LAIKGVAHFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPGVNGIIPMERLEAAMRDDTILLSLMHVNNEIGVV
HDIDAIGELCRSKGIIFHVDAAQSAGKLPIDVQITKVDLMSISGHKMYGPKGIGALYVRRKPRIRLESQMHGGGHERGMR
SGTLATHQIVGLGEAAAIAKADMESDNARIHALRDRLWNGIKDIEETYVNGDFEQRYCGNLNVSFNFVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLSDEMAHSSIRFSIGRFTTEEEVDYAIGIIQGSIGKLREMSPLWEMFKDGVDLSKVE
WVHH
>Mature_404_residues
MKLPIYFDYAATTPVDPRVAQKMMQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVADLINADHREIVFTSGATESNN
LAIKGVAHFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPGVNGIIPMERLEAAMRDDTILLSLMHVNNEIGVV
HDIDAIGELCRSKGIIFHVDAAQSAGKLPIDVQITKVDLMSISGHKMYGPKGIGALYVRRKPRIRLESQMHGGGHERGMR
SGTLATHQIVGLGEAAAIAKADMESDNARIHALRDRLWNGIKDIEETYVNGDFEQRYCGNLNVSFNFVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLSDEMAHSSIRFSIGRFTTEEEVDYAIGIIQGSIGKLREMSPLWEMFKDGVDLSKVE
WVHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=59.2039800995025, Blast_Score=506, Evalue=1e-143,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=32.7751196172249, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=78.4653465346535, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=181, Percent_Identity=33.7016574585635, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789175, Length=232, Percent_Identity=30.6034482758621, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=59.8014888337469, Blast_Score=496, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=32.0987654320988, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=57.1072319201995, Blast_Score=485, Evalue=1e-138,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=57.7114427860697, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_SHEAM (A1S544)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927170.1
- ProteinModelPortal:   A1S544
- SMR:   A1S544
- STRING:   A1S544
- GeneID:   4603545
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1293
- NMPDR:   fig|326297.7.peg.1220
- eggNOG:   COG1104
- HOGENOM:   HBG635316
- OMA:   SYVLRSM
- PhylomeDB:   A1S544
- ProtClustDB:   PRK14012
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 44801; Mature: 44801

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYFDYAATTPVDPRVAQKMMQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVAD
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADHREIVFTSGATESNNLAIKGVAHFYQKKGKHIITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLEPGVNGIIPMERLEAAMRDDTILLSLMHVNNEIGVVHDIDAIGELCRSKGIIFHVD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCEEECHHHHHHHHHHCCCEEEEE
AAQSAGKLPIDVQITKVDLMSISGHKMYGPKGIGALYVRRKPRIRLESQMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEEEHHHCCCCCHHCCCC
SGTLATHQIVGLGEAAAIAKADMESDNARIHALRDRLWNGIKDIEETYVNGDFEQRYCGN
CCCCHHHHEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
LNVSFNFVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLSDEMAHSSIRFSIGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEEEECCC
TTEEEVDYAIGIIQGSIGKLREMSPLWEMFKDGVDLSKVEWVHH
CCHHHHCEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure
MKLPIYFDYAATTPVDPRVAQKMMQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVAD
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADHREIVFTSGATESNNLAIKGVAHFYQKKGKHIITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLEPGVNGIIPMERLEAAMRDDTILLSLMHVNNEIGVVHDIDAIGELCRSKGIIFHVD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCEEECHHHHHHHHHHCCCEEEEE
AAQSAGKLPIDVQITKVDLMSISGHKMYGPKGIGALYVRRKPRIRLESQMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEEEHHHCCCCCHHCCCC
SGTLATHQIVGLGEAAAIAKADMESDNARIHALRDRLWNGIKDIEETYVNGDFEQRYCGN
CCCCHHHHEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
LNVSFNFVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLSDEMAHSSIRFSIGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEEEECCC
TTEEEVDYAIGIIQGSIGKLREMSPLWEMFKDGVDLSKVEWVHH
CCHHHHCEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA