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The map label for this gene is trmJ [H]

Identifier: 119774427

GI number: 119774427

Start: 1577595

End: 1578317

Strand: Direct

Name: trmJ [H]

Synonym: Sama_1290

Alternate gene names: 119774427

Gene position: 1577595-1578317 (Clockwise)

Preceding gene: 119774420

Following gene: 119774428

Centisome position: 36.64

GC content: 52.56

Gene sequence:

>723_bases
ATGCTCAGTAATATCCGCGTCGTCTTGGTAGGCACTTCCCATCCCGGTAACATAGGCTCCGTCGCTCGGGCTATGAAAAC
CATGGGTTTATCAAATCTTTATCTGGCAGAACCTCGCTGTGAGCCCGATGGTCAATCGGTAGCCTTGGCCGCTGGAGCCT
GCGATATTTTGAAAAATCTCACCCGGGTTGAGTCACTCCACGAAGCCATCAAGGACTGTGCCCTGGTCATTGGCACCTCG
GCACGCAGCCGTACGCTGGACTGGCCTCTGCTTGATCCCCGTCAGGCGGGTGGCAAACTGGCAGCTGAAGCCGTCAGTGG
CCCTGTGGCCATTGTGTTTGGCCGAGAGAATCATGGCCTCTATAACGAAGAACTTCAGGCCTGCAATTATCACGTCTTTA
TTCCAGCCAACCCCGAATACATGTCGCTTAATCTGGCGCAAGCGGTACAAATCATCTGTTATGAGACACGTATTGCCCAT
TTGGCGCTGGAAAATCAGGCGGCCGAAGTGACCGAATACCCCTTGATGGAAGAGAACGAGCGATTCTTTGAGCATCTCGA
AAGAACCCTGACCGGAACCGGGTTTATTATTAAGAATCATCCGGGGCAGGTGATGACCAAGTTAAGACGGCTATTTACCC
GCGCCCGGATAGAAAACCATGAGATGAACATTCTGCGTGGCATCCTGACTTCCATCGAAAAGGCCGTCGGCAGCAAGAAG
TAA

Upstream 100 bases:

>100_bases
CTGCCGGGTATATCAATTACAGATTTTCATATAAGGATTTAACCGCCGCTGTGGTAGACTGCACCGCTCAGGATTTTTAT
CAATTAAATTAAGTCGTTTC

Downstream 100 bases:

>100_bases
CCGAGCCCCGGGCTCAATGACAAGGAAACGACATGGGTGTGATATCCAGGCTGAAAGAAGACATTGCCTCCATTTACCAC
CGGGATCCGGCGGCGCGCAG

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MLSNIRVVLVGTSHPGNIGSVARAMKTMGLSNLYLAEPRCEPDGQSVALAAGACDILKNLTRVESLHEAIKDCALVIGTS
ARSRTLDWPLLDPRQAGGKLAAEAVSGPVAIVFGRENHGLYNEELQACNYHVFIPANPEYMSLNLAQAVQIICYETRIAH
LALENQAAEVTEYPLMEENERFFEHLERTLTGTGFIIKNHPGQVMTKLRRLFTRARIENHEMNILRGILTSIEKAVGSKK

Sequences:

>Translated_240_residues
MLSNIRVVLVGTSHPGNIGSVARAMKTMGLSNLYLAEPRCEPDGQSVALAAGACDILKNLTRVESLHEAIKDCALVIGTS
ARSRTLDWPLLDPRQAGGKLAAEAVSGPVAIVFGRENHGLYNEELQACNYHVFIPANPEYMSLNLAQAVQIICYETRIAH
LALENQAAEVTEYPLMEENERFFEHLERTLTGTGFIIKNHPGQVMTKLRRLFTRARIENHEMNILRGILTSIEKAVGSKK
>Mature_240_residues
MLSNIRVVLVGTSHPGNIGSVARAMKTMGLSNLYLAEPRCEPDGQSVALAAGACDILKNLTRVESLHEAIKDCALVIGTS
ARSRTLDWPLLDPRQAGGKLAAEAVSGPVAIVFGRENHGLYNEELQACNYHVFIPANPEYMSLNLAQAVQIICYETRIAH
LALENQAAEVTEYPLMEENERFFEHLERTLTGTGFIIKNHPGQVMTKLRRLFTRARIENHEMNILRGILTSIEKAVGSKK

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=238, Percent_Identity=64.2857142857143, Blast_Score=310, Evalue=8e-86,
Organism=Escherichia coli, GI1790865, Length=160, Percent_Identity=35, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26462; Mature: 26462

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNIRVVLVGTSHPGNIGSVARAMKTMGLSNLYLAEPRCEPDGQSVALAAGACDILKNL
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHH
TRVESLHEAIKDCALVIGTSARSRTLDWPLLDPRQAGGKLAAEAVSGPVAIVFGRENHGL
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHCCCHHHHHHCCCCEEEEEECCCCCC
YNEELQACNYHVFIPANPEYMSLNLAQAVQIICYETRIAHLALENQAAEVTEYPLMEENE
CHHHHHHCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHH
RFFEHLERTLTGTGFIIKNHPGQVMTKLRRLFTRARIENHEMNILRGILTSIEKAVGSKK
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLSNIRVVLVGTSHPGNIGSVARAMKTMGLSNLYLAEPRCEPDGQSVALAAGACDILKNL
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHH
TRVESLHEAIKDCALVIGTSARSRTLDWPLLDPRQAGGKLAAEAVSGPVAIVFGRENHGL
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHCCCHHHHHHCCCCEEEEEECCCCCC
YNEELQACNYHVFIPANPEYMSLNLAQAVQIICYETRIAHLALENQAAEVTEYPLMEENE
CHHHHHHCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHH
RFFEHLERTLTGTGFIIKNHPGQVMTKLRRLFTRARIENHEMNILRGILTSIEKAVGSKK
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA