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The map label for this gene is oprF [H]

Identifier: 119774051

GI number: 119774051

Start: 1104264

End: 1105370

Strand: Reverse

Name: oprF [H]

Synonym: Sama_0914

Alternate gene names: 119774051

Gene position: 1105370-1104264 (Counterclockwise)

Preceding gene: 119774052

Following gene: 119774050

Centisome position: 25.67

GC content: 53.21

Gene sequence:

>1107_bases
ATGATGAAAAACTCATTGAAAATTGCACTGCTCGCGTCGGCCCTGCCTTTCGGCGTCATGGCGTCTGATGAAGGCATGAG
CCCCTGGTATGTGGGTGGCGGTGTTGGTATCAACAACTATGAGCCAGCCTGTGACCAAAAAACCATGCGTTCCTGTGGTG
AAGACAGTCCTTATGCTTGGGACGTATTCGCTGGCTACCTGTTTAATGACTACTTTGGTGTCGAGCTGGGTTACCGTGAC
CTGGGCCGCGCCGAGTGGACTGACTACGCCAACAACCTGAATGACGTAGGCGCCAAGGGCACCACCCTGGGCTTGGTCGC
GTTCTGGCCATTTGCGAGCAAGTGGAGCCTGTCGGCTGAAGCCGGTGCCATGAACTACCTGGTGTACAACAACAAACGCT
GGGGCTCTAACTACTACAGCGACAGCGGTATTGCGCCATTCGTAGGTGCTGGTATCGGTTATAACTTCACCGATAACCTG
AAGCTGTCTGCCAAATATCGTCGTTACGAAAATCTCGACGAAGAGAAGTGGAACACGCTGGAAATGGAAAGCAACTATTG
GGGTCTGGAGCTGAGCTATCGCTTCGGCAGCAAGGCAGCTCCTGTCGTTGCCGCCCCCGTGGTTGTTGCACCTGTTGATT
CAGATAACGACGGTGTAAATGATGATCTGGACAAGTGCCCAGCCACACCTGCCGACCACAAGGTTGATGCATACGGCTGT
ACAGTTTACAAAGACGATGTGACCACCCACAACCTGGGCTCACTGCGCTTTGCCAACAACTCGTCTGTTATCAATCCATC
TTCCTACGAGTCTGTTGAGAAGTTGGCAAACTACATGAACCAACACCCAGGTTCAACCGTTGAAATCGGTGGCCACGCTT
CCAACGTCGGTAACCCAGACTACAACATGACCCTGTCTGAGAAGCGCGCCAATGCCGCAGCCAAGCTGCTGGTTGAGAAG
TATGGCATCAGTGCCAACCGTGTGACCGCCAAGGGTTATGGTATCACCCGTCCGGTAATGGATGGCAACACAGCTGAGGC
TCATGCGGCTAACCGCCGTGTTGAAGCGGTTGTGACTGGTTCTGAAAAGGTACCAGTACTCAAGTAA

Upstream 100 bases:

>100_bases
TGCTAAAACACAAAGGGAACACCACACGAAGTGCAAGCTATGCTCCAACATCCCAAAGAGCATTATGGTGGACATCATCA
AACAATGGACTTGGAGAATT

Downstream 100 bases:

>100_bases
TCTGTGATTTGTCACTGATAAAGCCACTGCCTGCAGTGGCTTTTTTCTTTGTATAATCCAATTTAAAGACCTTGCCTGAC
TCATTTCGAAATTTTAAGTC

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MMKNSLKIALLASALPFGVMASDEGMSPWYVGGGVGINNYEPACDQKTMRSCGEDSPYAWDVFAGYLFNDYFGVELGYRD
LGRAEWTDYANNLNDVGAKGTTLGLVAFWPFASKWSLSAEAGAMNYLVYNNKRWGSNYYSDSGIAPFVGAGIGYNFTDNL
KLSAKYRRYENLDEEKWNTLEMESNYWGLELSYRFGSKAAPVVAAPVVVAPVDSDNDGVNDDLDKCPATPADHKVDAYGC
TVYKDDVTTHNLGSLRFANNSSVINPSSYESVEKLANYMNQHPGSTVEIGGHASNVGNPDYNMTLSEKRANAAAKLLVEK
YGISANRVTAKGYGITRPVMDGNTAEAHAANRRVEAVVTGSEKVPVLK

Sequences:

>Translated_368_residues
MMKNSLKIALLASALPFGVMASDEGMSPWYVGGGVGINNYEPACDQKTMRSCGEDSPYAWDVFAGYLFNDYFGVELGYRD
LGRAEWTDYANNLNDVGAKGTTLGLVAFWPFASKWSLSAEAGAMNYLVYNNKRWGSNYYSDSGIAPFVGAGIGYNFTDNL
KLSAKYRRYENLDEEKWNTLEMESNYWGLELSYRFGSKAAPVVAAPVVVAPVDSDNDGVNDDLDKCPATPADHKVDAYGC
TVYKDDVTTHNLGSLRFANNSSVINPSSYESVEKLANYMNQHPGSTVEIGGHASNVGNPDYNMTLSEKRANAAAKLLVEK
YGISANRVTAKGYGITRPVMDGNTAEAHAANRRVEAVVTGSEKVPVLK
>Mature_368_residues
MMKNSLKIALLASALPFGVMASDEGMSPWYVGGGVGINNYEPACDQKTMRSCGEDSPYAWDVFAGYLFNDYFGVELGYRD
LGRAEWTDYANNLNDVGAKGTTLGLVAFWPFASKWSLSAEAGAMNYLVYNNKRWGSNYYSDSGIAPFVGAGIGYNFTDNL
KLSAKYRRYENLDEEKWNTLEMESNYWGLELSYRFGSKAAPVVAAPVVVAPVDSDNDGVNDDLDKCPATPADHKVDAYGC
TVYKDDVTTHNLGSLRFANNSSVINPSSYESVEKLANYMNQHPGSTVEIGGHASNVGNPDYNMTLSEKRANAAAKLLVEK
YGISANRVTAKGYGITRPVMDGNTAEAHAANRRVEAVVTGSEKVPVLK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787191, Length=380, Percent_Identity=24.7368421052632, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI48994946, Length=128, Percent_Identity=28.125, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786962, Length=78, Percent_Identity=37.1794871794872, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 40073; Mature: 40073

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKNSLKIALLASALPFGVMASDEGMSPWYVGGGVGINNYEPACDQKTMRSCGEDSPYAW
CCCCCEEEEEEECCCCCCEEECCCCCCCEEECCCCCCCCCCCCCCHHHHHHCCCCCCEEH
DVFAGYLFNDYFGVELGYRDLGRAEWTDYANNLNDVGAKGTTLGLVAFWPFASKWSLSAE
HHHHHHHHHCEEEEEECHHHCCCCHHHHHHCCHHHCCCCCCEEEEEEECCCCCCCCCCCC
AGAMNYLVYNNKRWGSNYYSDSGIAPFVGAGIGYNFTDNLKLSAKYRRYENLDEEKWNTL
CCEEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEHHHHHCCCCCHHCCEE
EMESNYWGLELSYRFGSKAAPVVAAPVVVAPVDSDNDGVNDDLDKCPATPADHKVDAYGC
EECCCEEEEEEEECCCCCCCCEEECCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCEEEE
TVYKDDVTTHNLGSLRFANNSSVINPSSYESVEKLANYMNQHPGSTVEIGGHASNVGNPD
EEEECCCCCCCCCEEEECCCCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
YNMTLSEKRANAAAKLLVEKYGISANRVTAKGYGITRPVMDGNTAEAHAANRRVEAVVTG
CEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCEEEEEEEC
SEKVPVLK
CCCCCCCC
>Mature Secondary Structure
MMKNSLKIALLASALPFGVMASDEGMSPWYVGGGVGINNYEPACDQKTMRSCGEDSPYAW
CCCCCEEEEEEECCCCCCEEECCCCCCCEEECCCCCCCCCCCCCCHHHHHHCCCCCCEEH
DVFAGYLFNDYFGVELGYRDLGRAEWTDYANNLNDVGAKGTTLGLVAFWPFASKWSLSAE
HHHHHHHHHCEEEEEECHHHCCCCHHHHHHCCHHHCCCCCCEEEEEEECCCCCCCCCCCC
AGAMNYLVYNNKRWGSNYYSDSGIAPFVGAGIGYNFTDNLKLSAKYRRYENLDEEKWNTL
CCEEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEHHHHHCCCCCHHCCEE
EMESNYWGLELSYRFGSKAAPVVAAPVVVAPVDSDNDGVNDDLDKCPATPADHKVDAYGC
EECCCEEEEEEEECCCCCCCCEEECCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCEEEE
TVYKDDVTTHNLGSLRFANNSSVINPSSYESVEKLANYMNQHPGSTVEIGGHASNVGNPD
EEEECCCCCCCCCEEEECCCCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
YNMTLSEKRANAAAKLLVEKYGISANRVTAKGYGITRPVMDGNTAEAHAANRRVEAVVTG
CEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCEEEEEEEC
SEKVPVLK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]