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The map label for this gene is cca [H]

Identifier: 119773971

GI number: 119773971

Start: 1016610

End: 1017893

Strand: Reverse

Name: cca [H]

Synonym: Sama_0834

Alternate gene names: 119773971

Gene position: 1017893-1016610 (Counterclockwise)

Preceding gene: 119773974

Following gene: 119773970

Centisome position: 23.64

GC content: 51.87

Gene sequence:

>1284_bases
ATGGATAACACATTGAAAACTTATCTGGTAGGCGGCGCAGTTCGGGATTCACTCCTCGGTCTTGACGTCAAAGACAGAGA
TTATATGGTGGTTGGCGCAACCGTAGAGGAAATGCTGGCGGCCGGGTACCGCCAGGTTGGTAAAGACTTTCCGGTATTTT
TGCACCCCAAAACCCAGGAAGAATATGCCCTTGCCCGCACCGAGCGAAAGAGCGGCACAGGTTACGGCGGCTTTGTCTGT
CATGCGAGCCCCGATGTCACCCTCGAAGAAGACCTCAAGCGCCGGGATCTGACCATCAACGCAATCGCCATGGCTGAGGA
TGGCAGTCTGCATGACCCCTGGGGCGGCGTAGCCGACCTTGAAGCAAGGTTGTTACGGCATGTCTGCGAGGCTTTTGTGG
AAGACCCCCTCAGGGTGCTTCGCGTTGCCCGCTTTGCCGCCCGTTTCGCGCCTCAGGGATTTACCGTCGCCCCTGACACC
CTGGCACTAATGCGCCAGCTGACTGAGAGTGGTGAGCTTTTGTACCTGACCGCAGAGCGTGTCTGGCAGGAGCTGGAGAA
GGTATTGGGCTGTGACAGTCCCCAGGTCTTTTTTGACGTGCTAAACCAATGTGGTGCGCTTGAGGTGCTGTTTCCGGAAA
TCCATGCTTTATTCGGTGTGCCTCAGCCTGAACAATGGCATCCTGAAATTGATACCGGGGTGCACACCATGATGGTGCTG
GAGCAGGCAGCCAAACTCACACCCAATAAGCAAGTCAGGTTTGCTGCCCTGGTACATGATCTTGGCAAGGGCGTCACCCC
AAAGGCACTTTGGCCCAAACACCATGGCCACGGTCAAAAGGGATTACCCCTCATCAAAGCACTCTGTGACAGATTTAGAG
TGCCCTCGGACTATAAAGAGCTGGCGCTTTGGGTGAGCGACGAACATCAGAATATTCACAATGCGTTTGAGCTCAGGGCC
GACACTATTCTAAGACTGTTTGATAAATTGGATCTCTGGCGCAAGCCTGAACGATTGCCTCTGGTATTGGATGCCTGCGA
GGCCGACATTAAAGGGCGAACCGGATTCGAAAATAACCCCTATCCTCAGAAAAAATATTTATTGGACTGTTATAAAGCGA
CCCAAAAAATTGACGTTCGGGAAATAATTGACGCGGGTTTTTCCGGGGGTGAAATCAAACATGAATTGGCGAAACGTCGG
CTTTCGGAAATAGGATTGATAAAAAACTCTGTCCAAAATCAATCAAAAGCTTCATCGGTGAAAAAATCTGACCTAGAAGC
GTGA

Upstream 100 bases:

>100_bases
ACCCATCAGCTTATTACCACCAAAAACCAAGTCTGAGTATCGCCCACAGGCTGTCACTGTTATTCCGGTGCTCGTGTTAA
GCTAACTGTATGACAGTTGG

Downstream 100 bases:

>100_bases
AATTTCTGCTTAAAACTTGTGGATTCTCTACTTGAAACTTGCAATTCACTGAATTTTAGATATGGTTATCAAGCGAATTG
ATGGATATTCGTAGCAAATT

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MDNTLKTYLVGGAVRDSLLGLDVKDRDYMVVGATVEEMLAAGYRQVGKDFPVFLHPKTQEEYALARTERKSGTGYGGFVC
HASPDVTLEEDLKRRDLTINAIAMAEDGSLHDPWGGVADLEARLLRHVCEAFVEDPLRVLRVARFAARFAPQGFTVAPDT
LALMRQLTESGELLYLTAERVWQELEKVLGCDSPQVFFDVLNQCGALEVLFPEIHALFGVPQPEQWHPEIDTGVHTMMVL
EQAAKLTPNKQVRFAALVHDLGKGVTPKALWPKHHGHGQKGLPLIKALCDRFRVPSDYKELALWVSDEHQNIHNAFELRA
DTILRLFDKLDLWRKPERLPLVLDACEADIKGRTGFENNPYPQKKYLLDCYKATQKIDVREIIDAGFSGGEIKHELAKRR
LSEIGLIKNSVQNQSKASSVKKSDLEA

Sequences:

>Translated_427_residues
MDNTLKTYLVGGAVRDSLLGLDVKDRDYMVVGATVEEMLAAGYRQVGKDFPVFLHPKTQEEYALARTERKSGTGYGGFVC
HASPDVTLEEDLKRRDLTINAIAMAEDGSLHDPWGGVADLEARLLRHVCEAFVEDPLRVLRVARFAARFAPQGFTVAPDT
LALMRQLTESGELLYLTAERVWQELEKVLGCDSPQVFFDVLNQCGALEVLFPEIHALFGVPQPEQWHPEIDTGVHTMMVL
EQAAKLTPNKQVRFAALVHDLGKGVTPKALWPKHHGHGQKGLPLIKALCDRFRVPSDYKELALWVSDEHQNIHNAFELRA
DTILRLFDKLDLWRKPERLPLVLDACEADIKGRTGFENNPYPQKKYLLDCYKATQKIDVREIIDAGFSGGEIKHELAKRR
LSEIGLIKNSVQNQSKASSVKKSDLEA
>Mature_427_residues
MDNTLKTYLVGGAVRDSLLGLDVKDRDYMVVGATVEEMLAAGYRQVGKDFPVFLHPKTQEEYALARTERKSGTGYGGFVC
HASPDVTLEEDLKRRDLTINAIAMAEDGSLHDPWGGVADLEARLLRHVCEAFVEDPLRVLRVARFAARFAPQGFTVAPDT
LALMRQLTESGELLYLTAERVWQELEKVLGCDSPQVFFDVLNQCGALEVLFPEIHALFGVPQPEQWHPEIDTGVHTMMVL
EQAAKLTPNKQVRFAALVHDLGKGVTPKALWPKHHGHGQKGLPLIKALCDRFRVPSDYKELALWVSDEHQNIHNAFELRA
DTILRLFDKLDLWRKPERLPLVLDACEADIKGRTGFENNPYPQKKYLLDCYKATQKIDVREIIDAGFSGGEIKHELAKRR
LSEIGLIKNSVQNQSKASSVKKSDLEA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=191, Percent_Identity=31.9371727748691, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1789436, Length=404, Percent_Identity=59.9009900990099, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87081691, Length=229, Percent_Identity=29.6943231441048, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281360112, Length=346, Percent_Identity=24.5664739884393, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357337, Length=346, Percent_Identity=24.5664739884393, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47915; Mature: 47915

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNTLKTYLVGGAVRDSLLGLDVKDRDYMVVGATVEEMLAAGYRQVGKDFPVFLHPKTQE
CCCCHHHHHCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCH
EYALARTERKSGTGYGGFVCHASPDVTLEEDLKRRDLTINAIAMAEDGSLHDPWGGVADL
HHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHH
EARLLRHVCEAFVEDPLRVLRVARFAARFAPQGFTVAPDTLALMRQLTESGELLYLTAER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCEEEEEHHH
VWQELEKVLGCDSPQVFFDVLNQCGALEVLFPEIHALFGVPQPEQWHPEIDTGVHTMMVL
HHHHHHHHHCCCCHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
EQAAKLTPNKQVRFAALVHDLGKGVTPKALWPKHHGHGQKGLPLIKALCDRFRVPSDYKE
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHH
LALWVSDEHQNIHNAFELRADTILRLFDKLDLWRKPERLPLVLDACEADIKGRTGFENNP
HEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCCCCCCCCCCCCC
YPQKKYLLDCYKATQKIDVREIIDAGFSGGEIKHELAKRRLSEIGLIKNSVQNQSKASSV
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSDLEA
HHHCCCC
>Mature Secondary Structure
MDNTLKTYLVGGAVRDSLLGLDVKDRDYMVVGATVEEMLAAGYRQVGKDFPVFLHPKTQE
CCCCHHHHHCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCH
EYALARTERKSGTGYGGFVCHASPDVTLEEDLKRRDLTINAIAMAEDGSLHDPWGGVADL
HHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHH
EARLLRHVCEAFVEDPLRVLRVARFAARFAPQGFTVAPDTLALMRQLTESGELLYLTAER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCEEEEEHHH
VWQELEKVLGCDSPQVFFDVLNQCGALEVLFPEIHALFGVPQPEQWHPEIDTGVHTMMVL
HHHHHHHHHCCCCHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
EQAAKLTPNKQVRFAALVHDLGKGVTPKALWPKHHGHGQKGLPLIKALCDRFRVPSDYKE
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHH
LALWVSDEHQNIHNAFELRADTILRLFDKLDLWRKPERLPLVLDACEADIKGRTGFENNP
HEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCCCCCCCCCCCCC
YPQKKYLLDCYKATQKIDVREIIDAGFSGGEIKHELAKRRLSEIGLIKNSVQNQSKASSV
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSDLEA
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA