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The map label for this gene is narQ [H]

Identifier: 119773785

GI number: 119773785

Start: 801916

End: 803628

Strand: Reverse

Name: narQ [H]

Synonym: Sama_0647

Alternate gene names: 119773785

Gene position: 803628-801916 (Counterclockwise)

Preceding gene: 119773787

Following gene: 119773784

Centisome position: 18.66

GC content: 52.13

Gene sequence:

>1713_bases
ATGAAACGTGGCAGTCTCACCTCCAAAATTCTCGGACTGATGATGGTGCTTATCCTGCTCTCCAGTGGGCTATCTATCTT
TGCGCTCGCCAACCTGTCGTACAGCCTTGGCGATGCCCGGGCAATCAATGCCTCCGGCTCACTGCGGATGCAAAGCTACC
GGCTGATGTTTTACGCCAATTCAGGCAGTGAAGAGGCCGAAGACAAAATCCGTGAGTTTGAAGCCACCCTCTACTCACCT
GCACTCAAAAACTCCCTTAACTGGAGTACCCCCCAACGCCTGAAAGACCAATATCTGCTGGTTATCGACAAATGGAAGGT
GATGAAATACTTCATCGAAGAGGAAAACTCGCGCCTGTATGCCTCTTCGTTAAAGGACTTTGTCGATACCATCGACCTGT
TCGTGCTGGAAACCGAACATCACGCCGCCTTTAAATTAAAATTGCTGGCAGCCAGCCAGTTACTGGGCCTGGGTATGATG
CTTGCCATTGCTCTGGTGGCGGTGCGCTACACCAAGCGCAAGGTGGTGGAGCCGTTAAACCAATTGATGCTCTCCGCCAA
CACCATTTCCAAAGGCAATTTCGATGTGGACATGCCCCAGACTGAGTATATCGAGCTGCAGGCGCTGGCCGATGCACTGG
ACTCCACCGCCAAGGAGTTGTCTGCGCTCTATGGGGATCTTGAGTCTCAGGTTCAGGAAAAAACCCTCGCACTGACCCGT
GCCAATCAGGAACTCAACTTTCTCTACGACAACCTGGTGATGCTGCACGGCAAAACCCTCGATTACCGCGCACTGAAAGC
AGCGTTGGATCAGCTCAAACAATATGAACAGCTGGACTATTTGCGACTGATTATTCAGGACGAAGGGGACATAGAAGAAG
CCATTGAAGCCGAAGGTGGCTGGCCGGCAGAGATTGAAGCTTCGTGCCGTTTTCCACTCACCTTTGAGCATACGGATTTG
GGCTACCTCGAGATGGTGTCAGACAAGGCGCCCAATCGCCCCCTGTTTGAGAACTTCGCCATCATGCTGGCCCGTTCCAT
TCTTATCCATCAATCAGCCGAGCAGCGTCAGCAACTGGCGTTGATGGAAGAGCGGGGTGTGATTGCCCGCGAGCTGCATG
ATTCGCTGGGGCAGGTGCTCTCGTTCCTCAAAATCCAAATCACCCTGCTCAGAAAAGGCCTTGACGACAGCTGCCGCAAC
CCTGAAGTCGAGCAACAACTCAATGAAATCAATGAAGGCGTGAGCACCGCCTATGTGCAGCTCAGGGAACTGCTTTCAAC
CTTCCGCCTCACCATCAAAGAGCCCAATCTCAAACACGCGCTCGAAGCCATGTTGGAGCAGCTTCGCAGTCAAACGCACA
CCAAGCTCACCCTGGATTACGGTATCCGCGAGCAACTGCTTGGCGCCAACCAGCACATTCACATACTGCAATTAACCCGC
GAAGCCACCTTAAATGCCATAAAACATGCCAACGCCAGTGAAATTGTGATTCAATGCGCTAAGAATAACGAAGGTATGAT
CAATATCAGTGTCAGTGATGATGGTGTTGGTATAGAACACCTCAAAGAGCGGGATCAGCATTTCGGCATTGGCATCATGC
ACGAGCGAGCCAGCAAGCTCGAGGGCCAACTGCGCTTTTCCAGCAATGAAGCCGGTGGTACAACTGTCACACTCACACTT
CCCCCACATCAGGAGTCAAGTCAACATGGGTAA

Upstream 100 bases:

>100_bases
GTTCACACTTTTGCATGAGGGCAGATGTGATAAAGAAAAAGCTAATGTAGAACATAGGCTTTAGTTGCAAACGCCAGCGC
CCTTGCCGACAGGAAATTTC

Downstream 100 bases:

>100_bases
GCCCTATTCAGTTCTGGTGGTAGACGACCACCCTCTGCTGCGCCGCGGTATCTGTCAGCTCATTGCATCTGATAAGGATT
TCACTCTGTTTGGGGAAGCA

Product: nitrate/nitrite sensor protein NarQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MKRGSLTSKILGLMMVLILLSSGLSIFALANLSYSLGDARAINASGSLRMQSYRLMFYANSGSEEAEDKIREFEATLYSP
ALKNSLNWSTPQRLKDQYLLVIDKWKVMKYFIEEENSRLYASSLKDFVDTIDLFVLETEHHAAFKLKLLAASQLLGLGMM
LAIALVAVRYTKRKVVEPLNQLMLSANTISKGNFDVDMPQTEYIELQALADALDSTAKELSALYGDLESQVQEKTLALTR
ANQELNFLYDNLVMLHGKTLDYRALKAALDQLKQYEQLDYLRLIIQDEGDIEEAIEAEGGWPAEIEASCRFPLTFEHTDL
GYLEMVSDKAPNRPLFENFAIMLARSILIHQSAEQRQQLALMEERGVIARELHDSLGQVLSFLKIQITLLRKGLDDSCRN
PEVEQQLNEINEGVSTAYVQLRELLSTFRLTIKEPNLKHALEAMLEQLRSQTHTKLTLDYGIREQLLGANQHIHILQLTR
EATLNAIKHANASEIVIQCAKNNEGMINISVSDDGVGIEHLKERDQHFGIGIMHERASKLEGQLRFSSNEAGGTTVTLTL
PPHQESSQHG

Sequences:

>Translated_570_residues
MKRGSLTSKILGLMMVLILLSSGLSIFALANLSYSLGDARAINASGSLRMQSYRLMFYANSGSEEAEDKIREFEATLYSP
ALKNSLNWSTPQRLKDQYLLVIDKWKVMKYFIEEENSRLYASSLKDFVDTIDLFVLETEHHAAFKLKLLAASQLLGLGMM
LAIALVAVRYTKRKVVEPLNQLMLSANTISKGNFDVDMPQTEYIELQALADALDSTAKELSALYGDLESQVQEKTLALTR
ANQELNFLYDNLVMLHGKTLDYRALKAALDQLKQYEQLDYLRLIIQDEGDIEEAIEAEGGWPAEIEASCRFPLTFEHTDL
GYLEMVSDKAPNRPLFENFAIMLARSILIHQSAEQRQQLALMEERGVIARELHDSLGQVLSFLKIQITLLRKGLDDSCRN
PEVEQQLNEINEGVSTAYVQLRELLSTFRLTIKEPNLKHALEAMLEQLRSQTHTKLTLDYGIREQLLGANQHIHILQLTR
EATLNAIKHANASEIVIQCAKNNEGMINISVSDDGVGIEHLKERDQHFGIGIMHERASKLEGQLRFSSNEAGGTTVTLTL
PPHQESSQHG
>Mature_570_residues
MKRGSLTSKILGLMMVLILLSSGLSIFALANLSYSLGDARAINASGSLRMQSYRLMFYANSGSEEAEDKIREFEATLYSP
ALKNSLNWSTPQRLKDQYLLVIDKWKVMKYFIEEENSRLYASSLKDFVDTIDLFVLETEHHAAFKLKLLAASQLLGLGMM
LAIALVAVRYTKRKVVEPLNQLMLSANTISKGNFDVDMPQTEYIELQALADALDSTAKELSALYGDLESQVQEKTLALTR
ANQELNFLYDNLVMLHGKTLDYRALKAALDQLKQYEQLDYLRLIIQDEGDIEEAIEAEGGWPAEIEASCRFPLTFEHTDL
GYLEMVSDKAPNRPLFENFAIMLARSILIHQSAEQRQQLALMEERGVIARELHDSLGQVLSFLKIQITLLRKGLDDSCRN
PEVEQQLNEINEGVSTAYVQLRELLSTFRLTIKEPNLKHALEAMLEQLRSQTHTKLTLDYGIREQLLGANQHIHILQLTR
EATLNAIKHANASEIVIQCAKNNEGMINISVSDDGVGIEHLKERDQHFGIGIMHERASKLEGQLRFSSNEAGGTTVTLTL
PPHQESSQHG

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=553, Percent_Identity=37.0705244122966, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1787474, Length=583, Percent_Identity=29.5025728987993, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI87082311, Length=195, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64168; Mature: 64168

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRGSLTSKILGLMMVLILLSSGLSIFALANLSYSLGDARAINASGSLRMQSYRLMFYAN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCEEEEEEEEEEEEC
SGSEEAEDKIREFEATLYSPALKNSLNWSTPQRLKDQYLLVIDKWKVMKYFIEEENSRLY
CCCHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHCCCEEEEEEHHHHHHHHHHCCCCEEH
ASSLKDFVDTIDLFVLETEHHAAFKLKLLAASQLLGLGMMLAIALVAVRYTKRKVVEPLN
HHHHHHHHHHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLMLSANTISKGNFDVDMPQTEYIELQALADALDSTAKELSALYGDLESQVQEKTLALTR
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANQELNFLYDNLVMLHGKTLDYRALKAALDQLKQYEQLDYLRLIIQDEGDIEEAIEAEGG
CCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCC
WPAEIEASCRFPLTFEHTDLGYLEMVSDKAPNRPLFENFAIMLARSILIHQSAEQRQQLA
CCCCEECCCCCCEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
LMEERGVIARELHDSLGQVLSFLKIQITLLRKGLDDSCRNPEVEQQLNEINEGVSTAYVQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LRELLSTFRLTIKEPNLKHALEAMLEQLRSQTHTKLTLDYGIREQLLGANQHIHILQLTR
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCEEEEEEHH
EATLNAIKHANASEIVIQCAKNNEGMINISVSDDGVGIEHLKERDQHFGIGIMHERASKL
HHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHC
EGQLRFSSNEAGGTTVTLTLPPHQESSQHG
CCEEEECCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKRGSLTSKILGLMMVLILLSSGLSIFALANLSYSLGDARAINASGSLRMQSYRLMFYAN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCEEEEEEEEEEEEC
SGSEEAEDKIREFEATLYSPALKNSLNWSTPQRLKDQYLLVIDKWKVMKYFIEEENSRLY
CCCHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHCCCEEEEEEHHHHHHHHHHCCCCEEH
ASSLKDFVDTIDLFVLETEHHAAFKLKLLAASQLLGLGMMLAIALVAVRYTKRKVVEPLN
HHHHHHHHHHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLMLSANTISKGNFDVDMPQTEYIELQALADALDSTAKELSALYGDLESQVQEKTLALTR
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANQELNFLYDNLVMLHGKTLDYRALKAALDQLKQYEQLDYLRLIIQDEGDIEEAIEAEGG
CCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCC
WPAEIEASCRFPLTFEHTDLGYLEMVSDKAPNRPLFENFAIMLARSILIHQSAEQRQQLA
CCCCEECCCCCCEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
LMEERGVIARELHDSLGQVLSFLKIQITLLRKGLDDSCRNPEVEQQLNEINEGVSTAYVQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LRELLSTFRLTIKEPNLKHALEAMLEQLRSQTHTKLTLDYGIREQLLGANQHIHILQLTR
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCEEEEEEHH
EATLNAIKHANASEIVIQCAKNNEGMINISVSDDGVGIEHLKERDQHFGIGIMHERASKL
HHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHC
EGQLRFSSNEAGGTTVTLTLPPHQESSQHG
CCEEEECCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]