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The map label for this gene is rimO [H]

Identifier: 119773625

GI number: 119773625

Start: 595731

End: 597212

Strand: Reverse

Name: rimO [H]

Synonym: Sama_0485

Alternate gene names: 119773625

Gene position: 597212-595731 (Counterclockwise)

Preceding gene: 119773627

Following gene: 119773623

Centisome position: 13.87

GC content: 56.82

Gene sequence:

>1482_bases
GTGCATCGGGCCCAGCGCAGTAAATCAAACCCGTCACGCAATATCGGAACGCGCACTTTGAACAAGACAGTCGAAACTTT
TGATCCCAAGCAGACTACCACGCTCGAGACCCCAGCCAAGACACTGGCAGCAGAAGCCAAGGCCAGCGACGGCACCATTC
AGGGCAACCGTATCGGTTTTGTCAGCCTGGGCTGCCCCAAGAATCTGGTGGACTCAGAGCGCATTCTGACCCAACTGCGT
ATCGACGGTTACGAGGTAACCAACAGCTACGACAATGCCGATCTGGTGATTGTAAACACCTGTGGCTTTATCGATGCCGC
CGTGGAAGAGTCGCTGGACGCCGTGCGTGAAGCCCTGGAAGAAAACGGCAAAGTAATAGTGACCGGCTGTTTGGGCGCCA
AAGAAAACCAAATTCGCGAAGTGCACCCCGATGTGCTGGAAATTACCGGTCCCCACAGCTATGAGGCCGTGCTGAATCAC
GTGCACAAGTATGTGCCAAAGCCAGAGCACAACCCCTTCACCTCGCTGATCCCTCAGACAGGTGTAAAGCTGACGCCCAA
GCACTACGCCTACCTGAAGATTTCTGAAGGCTGTGACAACCGCTGCACCTTCTGCATCATCCCTGCCCTGCGTGGCGATC
TGGACAGCCGTGGCGTAGGCAGTGTGCTCGATGAAGCCAAGCGTCTGGTAGAAAGCGGCGTGCAGGAAATTCTGGTGGTG
AGCCAGGACACGTCTGCCTACGGCAAAGACAAAGATGGCCGCACCGACTTCTGGAACGGCATGCCGGTAAAACAAGACAT
CACCAGCCTGGCGCGCCAGCTGGGCAAGATGGGCGCCTGGGTGCGTCTGCACTACGTATACCCATACCCATGGGTTGACG
ACTTGATCCCACTGATGGCCGAAGGACTCATTCTGCCTTACCTCGACCTGCCGCTGCAGCACGCCAGCCCACGCGTTCTG
AAGATGATGAAGCGCCCTGGCCGGGTTGACCGCCAGCTGGATGCCATCAAAAAATGGCGTGAGATCTGCCCGGATCTGGT
GATCCGCTCTACCTTTATCGTGGGCTTCCCCGGCGAGACTGAAGAAGACTTCGAAATGCTGCTGGACTTCCTGCGTGAAG
CACGTCTGGATCGCGTTGGCTGTTTCAAGTATTCCGAGGTGGATGGCGCCGTGGCCAACACCCTGGCTGAGCTAATCAGC
GAAGAAGTGAAAGAAGACAGATACGAGCGCTTTATGGAAGTGCAGGCCGAGATCAGCGCCGAGCGTCTGGCCCGTCTGGT
TGGTCGTGAGCTGGACATTCTGATTGATGACGTTGACGAAGAAGGCGCCATCGGCCGCTCTTACGCCGATGCCCCTGAAA
TCGATGGCATGGTGTTTATCAATGGCGAAACCGAGCTGACTCCCGGTGATATGGTGCGTGCCCGCATCGTTGCCTCAGAT
GAGCACGATCTCTGGGCCGAGTTGGTTGCCCTGGAAGACTAA

Upstream 100 bases:

>100_bases
CGTTATTTTGCTGAACAAATTTTCACCACACACCCTGTACCTTTCGGGCACTTGCGGTATAATCGCGCGCACTTTTTTAC
TGCCCACAGCCGAGGCCACT

Downstream 100 bases:

>100_bases
GATTCAGCACATTAAAAAACGGGCTTTATGCCCGTTTTTTGTTTTTACCGTGCCAAACGCCCTTAAATCAAAAGGGGCAT
CGCCCTTAAGCTGATGCCCC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MHRAQRSKSNPSRNIGTRTLNKTVETFDPKQTTTLETPAKTLAAEAKASDGTIQGNRIGFVSLGCPKNLVDSERILTQLR
IDGYEVTNSYDNADLVIVNTCGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLNH
VHKYVPKPEHNPFTSLIPQTGVKLTPKHYAYLKISEGCDNRCTFCIIPALRGDLDSRGVGSVLDEAKRLVESGVQEILVV
SQDTSAYGKDKDGRTDFWNGMPVKQDITSLARQLGKMGAWVRLHYVYPYPWVDDLIPLMAEGLILPYLDLPLQHASPRVL
KMMKRPGRVDRQLDAIKKWREICPDLVIRSTFIVGFPGETEEDFEMLLDFLREARLDRVGCFKYSEVDGAVANTLAELIS
EEVKEDRYERFMEVQAEISAERLARLVGRELDILIDDVDEEGAIGRSYADAPEIDGMVFINGETELTPGDMVRARIVASD
EHDLWAELVALED

Sequences:

>Translated_493_residues
MHRAQRSKSNPSRNIGTRTLNKTVETFDPKQTTTLETPAKTLAAEAKASDGTIQGNRIGFVSLGCPKNLVDSERILTQLR
IDGYEVTNSYDNADLVIVNTCGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLNH
VHKYVPKPEHNPFTSLIPQTGVKLTPKHYAYLKISEGCDNRCTFCIIPALRGDLDSRGVGSVLDEAKRLVESGVQEILVV
SQDTSAYGKDKDGRTDFWNGMPVKQDITSLARQLGKMGAWVRLHYVYPYPWVDDLIPLMAEGLILPYLDLPLQHASPRVL
KMMKRPGRVDRQLDAIKKWREICPDLVIRSTFIVGFPGETEEDFEMLLDFLREARLDRVGCFKYSEVDGAVANTLAELIS
EEVKEDRYERFMEVQAEISAERLARLVGRELDILIDDVDEEGAIGRSYADAPEIDGMVFINGETELTPGDMVRARIVASD
EHDLWAELVALED
>Mature_493_residues
MHRAQRSKSNPSRNIGTRTLNKTVETFDPKQTTTLETPAKTLAAEAKASDGTIQGNRIGFVSLGCPKNLVDSERILTQLR
IDGYEVTNSYDNADLVIVNTCGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLNH
VHKYVPKPEHNPFTSLIPQTGVKLTPKHYAYLKISEGCDNRCTFCIIPALRGDLDSRGVGSVLDEAKRLVESGVQEILVV
SQDTSAYGKDKDGRTDFWNGMPVKQDITSLARQLGKMGAWVRLHYVYPYPWVDDLIPLMAEGLILPYLDLPLQHASPRVL
KMMKRPGRVDRQLDAIKKWREICPDLVIRSTFIVGFPGETEEDFEMLLDFLREARLDRVGCFKYSEVDGAVANTLAELIS
EEVKEDRYERFMEVQAEISAERLARLVGRELDILIDDVDEEGAIGRSYADAPEIDGMVFINGETELTPGDMVRARIVASD
EHDLWAELVALED

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=475, Percent_Identity=26.1052631578947, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=25, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI28872782, Length=485, Percent_Identity=24.7422680412371, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1787057, Length=433, Percent_Identity=73.6720554272517, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=397, Percent_Identity=29.2191435768262, Blast_Score=138, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17553146, Length=486, Percent_Identity=25.7201646090535, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71996771, Length=223, Percent_Identity=29.5964125560538, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=24.6315789473684, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=25.3275109170306, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 55087; Mature: 55087

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRAQRSKSNPSRNIGTRTLNKTVETFDPKQTTTLETPAKTLAAEAKASDGTIQGNRIGF
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEECCEEEE
VSLGCPKNLVDSERILTQLRIDGYEVTNSYDNADLVIVNTCGFIDAAVEESLDAVREALE
EEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
ENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLNHVHKYVPKPEHNPFTSLIPQT
CCCCEEEEECCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCC
GVKLTPKHYAYLKISEGCDNRCTFCIIPALRGDLDSRGVGSVLDEAKRLVESGVQEILVV
CCEECCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEE
SQDTSAYGKDKDGRTDFWNGMPVKQDITSLARQLGKMGAWVRLHYVYPYPWVDDLIPLMA
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHH
EGLILPYLDLPLQHASPRVLKMMKRPGRVDRQLDAIKKWREICPDLVIRSTFIVGFPGET
CCCCCCHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
EEDFEMLLDFLREARLDRVGCFKYSEVDGAVANTLAELISEEVKEDRYERFMEVQAEISA
HHHHHHHHHHHHHHHHHHHCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERLARLVGRELDILIDDVDEEGAIGRSYADAPEIDGMVFINGETELTPGDMVRARIVASD
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHEEEEEECC
EHDLWAELVALED
CHHHHHHHHHCCC
>Mature Secondary Structure
MHRAQRSKSNPSRNIGTRTLNKTVETFDPKQTTTLETPAKTLAAEAKASDGTIQGNRIGF
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEECCEEEE
VSLGCPKNLVDSERILTQLRIDGYEVTNSYDNADLVIVNTCGFIDAAVEESLDAVREALE
EEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
ENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLNHVHKYVPKPEHNPFTSLIPQT
CCCCEEEEECCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCC
GVKLTPKHYAYLKISEGCDNRCTFCIIPALRGDLDSRGVGSVLDEAKRLVESGVQEILVV
CCEECCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEE
SQDTSAYGKDKDGRTDFWNGMPVKQDITSLARQLGKMGAWVRLHYVYPYPWVDDLIPLMA
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHH
EGLILPYLDLPLQHASPRVLKMMKRPGRVDRQLDAIKKWREICPDLVIRSTFIVGFPGET
CCCCCCHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
EEDFEMLLDFLREARLDRVGCFKYSEVDGAVANTLAELISEEVKEDRYERFMEVQAEISA
HHHHHHHHHHHHHHHHHHHCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERLARLVGRELDILIDDVDEEGAIGRSYADAPEIDGMVFINGETELTPGDMVRARIVASD
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHEEEEEECC
EHDLWAELVALED
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]