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The map label for this gene is mraW

Identifier: 119773487

GI number: 119773487

Start: 414936

End: 415877

Strand: Direct

Name: mraW

Synonym: Sama_0346

Alternate gene names: 119773487

Gene position: 414936-415877 (Clockwise)

Preceding gene: 119773486

Following gene: 119773488

Centisome position: 9.64

GC content: 53.4

Gene sequence:

>942_bases
ATGAGCCAGGATTTTGCCCATTTATCCGTACTGCTTGCTGAGACCGTTGACGGGCTCAATATTCGAGCTGACGGCATTTA
TATTGACGGTACTTTTGGCCGTGGCGGGCATTCCCGTGAGGTATTGTCGCGTCTGGGGGTTGATGGACGCCTGATTGCTA
TCGACAGAGATCCTCAGGCCATCAAGGCGGCAGAGCAGTTTGCAAACGACAGTCGCTTCCAGATAGTACACGGCGGCTTT
GGTCAACTGGCCAACTACGTTGAAGAGCTGGGCCTTGTTGGCAAGATTGATGGGGTCCTGCTGGACTTGGGCGTATCCTC
TCCACAGCTTGACGATGCTGAACGGGGTTTCAGCTTTTTACGCGACGGTCCATTGGATATGCGCATGGATAACTCCCAGG
GTGAAACGGCGGCCCAATGGTTGGCCCGGGCTGAAATTGAAGACATGGCATGGGTGTTTAAAACCTATGGCGAAGAGCGA
AACGCACGCCACATCGCTCGCTGTATCGCAGCCGATAGAGAAAAAACGCCTTTTACCCGGACCAAACAACTGGCAGACCT
GATAGCCAGAGTCGCTAAAAGTAAAGAGCGTAACAAGCACCCTGCAACCCGCGTTTTCCAGGCAATCCGCATTTACATCA
ACAGCGAACTTGAACAGATCGATCAGGCGCTGGAAGGGGCACTCAAGGTGCTCGCTCCCCACGGCCGTCTTTCCATCATC
AGTTTCCACTCTTTGGAAGATCGCATTGTGAAGCGTTTTATCCGTCGACACAGCCAAGGGGAGGAGTTACCCCATGGTTT
GCCGGTAACCGAAGCGCAGCTGAATAAATCACGTACTCTGATGCCCGTGGGAAAAGCCATGAAGCCGTCAGATGTAGAAA
TTGAACAAAATGCCAGGGCGCGCAGCTCGGTACTGAGAGTGGCAGAGCGCCTGCCATTCTGA

Upstream 100 bases:

>100_bases
AGTGCGCAGGATGTTATCCGCAGCGAGGATCTGGCAGCCAATGAGCGTCTTGCAGATTTTTCGCTCTGATGAGTGAACCC
AAGGATATAAGAAGAACGCG

Downstream 100 bases:

>100_bases
GGAGTTACCGATGAGTAAGCCGCTGAGTTTGCCACGTATTGTGTTGATGGACCTGTGGCATCACAAATGGACGTTTTTAC
TCGCGCTCTGTGTGATGTTG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSQDFAHLSVLLAETVDGLNIRADGIYIDGTFGRGGHSREVLSRLGVDGRLIAIDRDPQAIKAAEQFANDSRFQIVHGGF
GQLANYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEER
NARHIARCIAADREKTPFTRTKQLADLIARVAKSKERNKHPATRVFQAIRIYINSELEQIDQALEGALKVLAPHGRLSII
SFHSLEDRIVKRFIRRHSQGEELPHGLPVTEAQLNKSRTLMPVGKAMKPSDVEIEQNARARSSVLRVAERLPF

Sequences:

>Translated_313_residues
MSQDFAHLSVLLAETVDGLNIRADGIYIDGTFGRGGHSREVLSRLGVDGRLIAIDRDPQAIKAAEQFANDSRFQIVHGGF
GQLANYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEER
NARHIARCIAADREKTPFTRTKQLADLIARVAKSKERNKHPATRVFQAIRIYINSELEQIDQALEGALKVLAPHGRLSII
SFHSLEDRIVKRFIRRHSQGEELPHGLPVTEAQLNKSRTLMPVGKAMKPSDVEIEQNARARSSVLRVAERLPF
>Mature_312_residues
SQDFAHLSVLLAETVDGLNIRADGIYIDGTFGRGGHSREVLSRLGVDGRLIAIDRDPQAIKAAEQFANDSRFQIVHGGFG
QLANYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEERN
ARHIARCIAADREKTPFTRTKQLADLIARVAKSKERNKHPATRVFQAIRIYINSELEQIDQALEGALKVLAPHGRLSIIS
FHSLEDRIVKRFIRRHSQGEELPHGLPVTEAQLNKSRTLMPVGKAMKPSDVEIEQNARARSSVLRVAERLPF

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=38.5294117647059, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI165377202, Length=194, Percent_Identity=40.7216494845361, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=63.7820512820513, Blast_Score=402, Evalue=1e-113,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=37.4622356495468, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=37.4622356495468, Blast_Score=177, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_SHEAM (A1S2F1)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926227.1
- ProteinModelPortal:   A1S2F1
- SMR:   A1S2F1
- STRING:   A1S2F1
- GeneID:   4602602
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0346
- NMPDR:   fig|326297.7.peg.328
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   A1S2F1
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34914; Mature: 34783

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 80-80 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDFAHLSVLLAETVDGLNIRADGIYIDGTFGRGGHSREVLSRLGVDGRLIAIDRDPQA
CCCHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCHH
IKAAEQFANDSRFQIVHGGFGQLANYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFL
HHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEERNARHIARCIAADREKTPFTR
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
TKQLADLIARVAKSKERNKHPATRVFQAIRIYINSELEQIDQALEGALKVLAPHGRLSII
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SFHSLEDRIVKRFIRRHSQGEELPHGLPVTEAQLNKSRTLMPVGKAMKPSDVEIEQNARA
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
RSSVLRVAERLPF
HHHHHHHHHHCCC
>Mature Secondary Structure 
SQDFAHLSVLLAETVDGLNIRADGIYIDGTFGRGGHSREVLSRLGVDGRLIAIDRDPQA
CCHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCHH
IKAAEQFANDSRFQIVHGGFGQLANYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFL
HHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEERNARHIARCIAADREKTPFTR
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
TKQLADLIARVAKSKERNKHPATRVFQAIRIYINSELEQIDQALEGALKVLAPHGRLSII
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SFHSLEDRIVKRFIRRHSQGEELPHGLPVTEAQLNKSRTLMPVGKAMKPSDVEIEQNARA
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
RSSVLRVAERLPF
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA