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The map label for this gene is yaaJ [C]

Identifier: 119773425

GI number: 119773425

Start: 331946

End: 333379

Strand: Direct

Name: yaaJ [C]

Synonym: Sama_0284

Alternate gene names: 119773425

Gene position: 331946-333379 (Clockwise)

Preceding gene: 119773423

Following gene: 119773426

Centisome position: 7.71

GC content: 59.07

Gene sequence:

>1434_bases
ATGACCGCCGACACTTCCTTTGATTTTGCCGCTGCCGCCGCCCGCTTTGCCGCCTTTGCCTGGGGGCCACACATGCTGGT
TCTGCTGGTGGGTGGCGGGCTGTTTTTCCTTATTTACTCTGGGCTTGCGCCATTTCGTTATTTAAAGCATGCCATCGACA
TAGTGCGAGGCAAGTACCCCGAAGCCGATGCCGCAGGCAATATCAGTCATGCCGGGGCGCTGTCCAGCGCGATGGCGGGC
ACTGTGGGCATGGGCAATATTGGTGGGGTAGCGCTGGCGATAGTCGCCGGTGGCCCCGGCGCTATTTTTTGGATGTGGGT
CAGCGCGCTGGTTGGCATGGCCACCAAGTTTTTTACCTGCTCACTGGCGATTATGTATCGCGGCACCGATGAAACAGGGC
GGGTGCGCGGTGGCCCCATGTACATCATTACCCACGGGCTTGGACAAAAATGGAAGCCGCTGGCGGTGTTCTTTTGTCTG
GTGGGGCTGGTGGGTAATTTCCCGCTGTTCAACACCAATCAGCTGGTGCAAATCCTCAAGGAATACCTGTTTTTATCCCC
CGGCGACACGGGCGTTGCCGTGGGCAGTGGCGAGCATTTTGCCCTGGAGCTGGGCCTGGGGCTCGCCATCATGAGTCTGG
TGGCGCTGGTGATCCTGGGCGGGATTAAGCGGATAGCGGCGGTGGCCATCAGAGTGGTGCCTACCATGATTCTGCTGTAC
GTGGGCTGCGCGCTTTATGTGATTTTAAGCCACCTTGGCGATGTGCCCATGCACCTGTGGCGCATAGTGGAAGATGCCTT
TTCGGTGCAGTCGGTGGCTGGGGGCATTTTGGGCACCATGCTCACCGGGGTGAAGCGGGCCGCTTTTTCCAACGAGGCGG
GCATAGGCACTGAGGTGATGGTACACGGCAACGCCAAAACCTCTGAGCCGGTGAAAGAAGGTCTGGTGGCCATGCTCGGC
CCCGCCATCGACACCCTCTTGGTGTGCACGGCCACGGCGCTGATTATTCTTATCTCCGGCGTGTGGGAGCAGGGCGGTGC
CAATGGCATCAGCCTGTCCAGTGCGGCCTTTGCCGCCACCATGCCGGGCATTGGCCCCTATCTTTTACTGGTGTGTGTTA
GCTTTTTTGCCATAACCACCATCTTCACCCAGGCCTTTTATGGCAGCCAATGCTTTGCGTTTTTGTTTGGCGCAAGGCGC
GAGCGCTGGTACTTGTACCTGTATTTGCTGGCGATTTTATTTGCCGCCACCAACAGCCTCACCGACATAGTCAACATCAT
CGACGGCGCCTACGGCCTGATGGCCATCCCCACCATGACAGCAGCCTTACTGCTGGCGCCTCGGGTGCGCGCAGCGGCAA
AGGATTACTTTCGCAGGTTGCGCTCCCAAGGGGGCGAGTTGGTTGGCCGTGATGACCCTGTGGAGCGGCGCTGA

Upstream 100 bases:

>100_bases
TCCGTATTCCGTATTCCGTATTCCCCATTCCTCAATCCCTTTCTATCCACGGCGTAGGCTGATAAGTTAGCGGGATGCCA
GTTAACATAATGTCAACAAG

Downstream 100 bases:

>100_bases
TAATAGTCAGCACAACTTTTGCCACAGCCGCTTTGGGCGTACAATCGGCGGCCCTGATGCCGTAATTAACAGGAAGTGAT
ATGTCCAACTGCTCGCCGCT

Product: sodium/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTADTSFDFAAAAARFAAFAWGPHMLVLLVGGGLFFLIYSGLAPFRYLKHAIDIVRGKYPEADAAGNISHAGALSSAMAG
TVGMGNIGGVALAIVAGGPGAIFWMWVSALVGMATKFFTCSLAIMYRGTDETGRVRGGPMYIITHGLGQKWKPLAVFFCL
VGLVGNFPLFNTNQLVQILKEYLFLSPGDTGVAVGSGEHFALELGLGLAIMSLVALVILGGIKRIAAVAIRVVPTMILLY
VGCALYVILSHLGDVPMHLWRIVEDAFSVQSVAGGILGTMLTGVKRAAFSNEAGIGTEVMVHGNAKTSEPVKEGLVAMLG
PAIDTLLVCTATALIILISGVWEQGGANGISLSSAAFAATMPGIGPYLLLVCVSFFAITTIFTQAFYGSQCFAFLFGARR
ERWYLYLYLLAILFAATNSLTDIVNIIDGAYGLMAIPTMTAALLLAPRVRAAAKDYFRRLRSQGGELVGRDDPVERR

Sequences:

>Translated_477_residues
MTADTSFDFAAAAARFAAFAWGPHMLVLLVGGGLFFLIYSGLAPFRYLKHAIDIVRGKYPEADAAGNISHAGALSSAMAG
TVGMGNIGGVALAIVAGGPGAIFWMWVSALVGMATKFFTCSLAIMYRGTDETGRVRGGPMYIITHGLGQKWKPLAVFFCL
VGLVGNFPLFNTNQLVQILKEYLFLSPGDTGVAVGSGEHFALELGLGLAIMSLVALVILGGIKRIAAVAIRVVPTMILLY
VGCALYVILSHLGDVPMHLWRIVEDAFSVQSVAGGILGTMLTGVKRAAFSNEAGIGTEVMVHGNAKTSEPVKEGLVAMLG
PAIDTLLVCTATALIILISGVWEQGGANGISLSSAAFAATMPGIGPYLLLVCVSFFAITTIFTQAFYGSQCFAFLFGARR
ERWYLYLYLLAILFAATNSLTDIVNIIDGAYGLMAIPTMTAALLLAPRVRAAAKDYFRRLRSQGGELVGRDDPVERR
>Mature_476_residues
TADTSFDFAAAAARFAAFAWGPHMLVLLVGGGLFFLIYSGLAPFRYLKHAIDIVRGKYPEADAAGNISHAGALSSAMAGT
VGMGNIGGVALAIVAGGPGAIFWMWVSALVGMATKFFTCSLAIMYRGTDETGRVRGGPMYIITHGLGQKWKPLAVFFCLV
GLVGNFPLFNTNQLVQILKEYLFLSPGDTGVAVGSGEHFALELGLGLAIMSLVALVILGGIKRIAAVAIRVVPTMILLYV
GCALYVILSHLGDVPMHLWRIVEDAFSVQSVAGGILGTMLTGVKRAAFSNEAGIGTEVMVHGNAKTSEPVKEGLVAMLGP
AIDTLLVCTATALIILISGVWEQGGANGISLSSAAFAATMPGIGPYLLLVCVSFFAITTIFTQAFYGSQCFAFLFGARRE
RWYLYLYLLAILFAATNSLTDIVNIIDGAYGLMAIPTMTAALLLAPRVRAAAKDYFRRLRSQGGELVGRDDPVERR

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=455, Percent_Identity=33.4065934065934, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 50472; Mature: 50341

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADTSFDFAAAAARFAAFAWGPHMLVLLVGGGLFFLIYSGLAPFRYLKHAIDIVRGKYP
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
EADAAGNISHAGALSSAMAGTVGMGNIGGVALAIVAGGPGAIFWMWVSALVGMATKFFTC
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
SLAIMYRGTDETGRVRGGPMYIITHGLGQKWKPLAVFFCLVGLVGNFPLFNTNQLVQILK
EEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EYLFLSPGDTGVAVGSGEHFALELGLGLAIMSLVALVILGGIKRIAAVAIRVVPTMILLY
HHHEECCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGCALYVILSHLGDVPMHLWRIVEDAFSVQSVAGGILGTMLTGVKRAAFSNEAGIGTEVM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VHGNAKTSEPVKEGLVAMLGPAIDTLLVCTATALIILISGVWEQGGANGISLSSAAFAAT
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MPGIGPYLLLVCVSFFAITTIFTQAFYGSQCFAFLFGARRERWYLYLYLLAILFAATNSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TDIVNIIDGAYGLMAIPTMTAALLLAPRVRAAAKDYFRRLRSQGGELVGRDDPVERR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TADTSFDFAAAAARFAAFAWGPHMLVLLVGGGLFFLIYSGLAPFRYLKHAIDIVRGKYP
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
EADAAGNISHAGALSSAMAGTVGMGNIGGVALAIVAGGPGAIFWMWVSALVGMATKFFTC
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
SLAIMYRGTDETGRVRGGPMYIITHGLGQKWKPLAVFFCLVGLVGNFPLFNTNQLVQILK
EEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EYLFLSPGDTGVAVGSGEHFALELGLGLAIMSLVALVILGGIKRIAAVAIRVVPTMILLY
HHHEECCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGCALYVILSHLGDVPMHLWRIVEDAFSVQSVAGGILGTMLTGVKRAAFSNEAGIGTEVM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VHGNAKTSEPVKEGLVAMLGPAIDTLLVCTATALIILISGVWEQGGANGISLSSAAFAAT
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MPGIGPYLLLVCVSFFAITTIFTQAFYGSQCFAFLFGARRERWYLYLYLLAILFAATNSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TDIVNIIDGAYGLMAIPTMTAALLLAPRVRAAAKDYFRRLRSQGGELVGRDDPVERR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]