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The map label for this gene is rpoA

Identifier: 119773379

GI number: 119773379

Start: 264078

End: 265067

Strand: Direct

Name: rpoA

Synonym: Sama_0238

Alternate gene names: 119773379

Gene position: 264078-265067 (Clockwise)

Preceding gene: 119773378

Following gene: 119773380

Centisome position: 6.13

GC content: 52.32

Gene sequence:

>990_bases
ATGCAGGGTTCTGTTACAGAATTTCTTAAACCGCGTCTCGTGGACATCGAGCAGGTTAGCCCAACTCGTGCCAAAGTTAC
CCTGGAACCGCTTGAGCGTGGTTTCGGTCACACTTTAGGTAACGCGTTGCGTCGCATCCTTCTGTCGTCCATGCCTGGCT
GCGCGGTCACCGAAGTTGAAATCGACGGTGTACTGCACGAATACAGCAGCAAGGAAGGCGTTCAGGAAGACATCCTCGAG
ATCTTGTTGAACCTGAAAGGTTTGGCAGTGATTCTTGAGGGCAAAGACGAGGCTATGCTCACTCTGAGCAAGTCCGGCGC
AGGCCCTGTTACTGCAGCAGACATCACCCATGATGGTGATGTTACTATCGCCAACCCTGATCATGTTATCTGTCACCTGA
CCGGTAATCATGACATCAGCATGCGTATCCGCGTTGAGCGTGGTCGTGGTTATGTGCCGGCTTCTGCCCGTGCACAGAAC
GAAGACGATGATCGCCCAATCGGTCGTCTGCTGGTTGACGCTTCTTTCTCACCGGTTGCCCGTATTGCCTACAATGTAGA
AGCGGCCCGTGTTGAGCAGCGTACTGACCTGGACAAGCTGGTAATCGATATGACCACTAATGGTACAATCGATCCAGAAG
AGGCTATCCGTCGCTCTGCAACTATTCTGGCTGAACAGCTGGATGCGTTTGTAGAACTGCGTGATGTGTCTGTTCCAGAG
CAGAAGGAAGAGAAGCCAGAGTTCGATCCAATCCTGCTGCGTCCTGTCGACGATTTAGAGCTTACTGTACGTTCGGCCAA
CTGCTTGAAGGCCGAAGCGATTCATTACATCGGTGATCTGGTTCAACGCACAGAAGTTGAGCTGCTCAAGACTCCTAACC
TGGGTAAGAAATCTCTTACCGAGATCAAGGACGTATTGGCTTCTCGCGGTCTGTCGTTGGGTATGCGTCTGGAAAACTGG
CCTCCAGCCAGCTTGGCAGACGACCTGTAA

Upstream 100 bases:

>100_bases
CAAGCGTCTGCCAGAGCGTAGCGATTTGTCTGCGGATATTAACGAACAGCTGATCGTCGAGCTTTACTCTAAGTAAGGCT
AACAAACAAGAGAGGACACA

Downstream 100 bases:

>100_bases
TACCGGATTGGTACAGAGATAGTTTATAAGGATTAGGTCATGCGCCATCGTAAGAGTGGTCGTCAACTTAACCGCAACAG
CAGCCACCGTCAGGCTATGT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MQGSVTEFLKPRLVDIEQVSPTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILE
ILLNLKGLAVILEGKDEAMLTLSKSGAGPVTAADITHDGDVTIANPDHVICHLTGNHDISMRIRVERGRGYVPASARAQN
EDDDRPIGRLLVDASFSPVARIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVSVPE
QKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENW
PPASLADDL

Sequences:

>Translated_329_residues
MQGSVTEFLKPRLVDIEQVSPTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILE
ILLNLKGLAVILEGKDEAMLTLSKSGAGPVTAADITHDGDVTIANPDHVICHLTGNHDISMRIRVERGRGYVPASARAQN
EDDDRPIGRLLVDASFSPVARIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVSVPE
QKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENW
PPASLADDL
>Mature_329_residues
MQGSVTEFLKPRLVDIEQVSPTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILE
ILLNLKGLAVILEGKDEAMLTLSKSGAGPVTAADITHDGDVTIANPDHVICHLTGNHDISMRIRVERGRGYVPASARAQN
EDDDRPIGRLLVDASFSPVARIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVSVPE
QKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENW
PPASLADDL

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=329, Percent_Identity=88.1458966565349, Blast_Score=582, Evalue=1e-168,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_SHEAM (A1S243)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926119.1
- ProteinModelPortal:   A1S243
- STRING:   A1S243
- GeneID:   4602494
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0238
- NMPDR:   fig|326297.7.peg.228
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   A1S243
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36105; Mature: 36105

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGSVTEFLKPRLVDIEQVSPTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
CCCCHHHHHCCCCCCHHHCCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVLHEYSSKEGVQEDILEILLNLKGLAVILEGKDEAMLTLSKSGAGPVTAADITHDGD
HHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCEEEEEECCCCC
VTIANPDHVICHLTGNHDISMRIRVERGRGYVPASARAQNEDDDRPIGRLLVDASFSPVA
EEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCHH
RIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVSVPE
HHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLT
HHCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKDVLASRGLSLGMRLENWPPASLADDL
HHHHHHHHCCCEECCEECCCCCCCCCCCC
>Mature Secondary Structure
MQGSVTEFLKPRLVDIEQVSPTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
CCCCHHHHHCCCCCCHHHCCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVLHEYSSKEGVQEDILEILLNLKGLAVILEGKDEAMLTLSKSGAGPVTAADITHDGD
HHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCEEEEEECCCCC
VTIANPDHVICHLTGNHDISMRIRVERGRGYVPASARAQNEDDDRPIGRLLVDASFSPVA
EEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCHH
RIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVSVPE
HHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLT
HHCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKDVLASRGLSLGMRLENWPPASLADDL
HHHHHHHHCCCEECCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA