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The map label for this gene is proS

Identifier: 119717421

GI number: 119717421

Start: 3395195

End: 3396964

Strand: Reverse

Name: proS

Synonym: Noca_3197

Alternate gene names: 119717421

Gene position: 3396964-3395195 (Counterclockwise)

Preceding gene: 119717422

Following gene: 119717420

Centisome position: 68.13

GC content: 71.13

Gene sequence:

>1770_bases
ATGATCATGCGGATGTCGAGCCTGTTCGTGCGGACCCTGCGAGAGGACCCCGCGGACGCCGAGGTGCCGTCGCACCGGCT
GCTCGTCCGCGCCGGCTACATCCGCCGCGCCGCCCCCGGCATCTACACCTGGCTGCCGCTGGGCCTGAGGGTGCTCCGCA
AGATCGAGAACGTCATCCGCGAGGAGATGGACGCGATCGGCGCCCAGGAGATGCTGTTCCCGGCGCTGCTGCCGCGCGAG
CCCTACGAGGCGACCAACCGGTGGACGGAGTACGGCGACGGCATCTTCCGGCTCCAGGACCGCAAGGGCGCCGACTACCT
CCTCGGCCCGACCCACGAGGAGATGTTCACGCTCGTCGTGAAGGACCTCTACTCCTCCTACAAGGACCTGCCGCTCTCGA
TCTACCAGATCCAGACGAAGTACCGCGACGAGGCGCGACCCCGCGCCGGGCTGCTGCGCGGGCGCGAGTTCGTGATGAAG
GACTCCTACTCGTTCGACGTCGACGACGCCGGCCTCGACGTGAGCTACCAGAAGCACCGCGACGCCTACGTGCGGATCTT
CGACCGCCTCGGGTTCGAGTACGTCATCGTCGAGGCGATGTCCGGCGCGATGGGCGGGTCGAAGTCCGAGGAGTTCCTCG
CCAAGGCGAGCGTCGGAGAGGACACCTACGTGCGCTGCACGTTGTGCGACTACGCCGCGAACGTCGAGGCCGTGCACTCG
CCGCCGATCCCGCCCGTGCCGTACGACGACGCGCCCGCCGCCCACGCCGAGCAGACTCCCGACACGCCCACGATCGAGAC
GCTCGTGGCCCACCTCAACGAGCGGTTCCCCCGCGAGGACCGCCCCTGGGCGGCGTCGGACACCCTGAAGAACGTGGTGT
TCTCGGTCCACCACCCCGACGGCGCCACCGAGGCGCTGGCCGTCGGCCTGCCCGGCGACCGCGAGGTCGACGCCAAGCGG
CTCGAGGCCCACCTCGGCGAGGGCGTCGTCTTCGAGCCGTTCGGCGAGGCGGACTTCGCGGCCCGCCCGACCCTGGCCAA
GGGCTACATCGGCCCCGGGGCGCTGGGGGAGAAGAAGCCGGCCGGCGTGCGCTACCTGCTCGACCCACGGGTCGTCGAGG
GCACCCGCTGGGTGACCGGAGCCGACGCCGCCGGCAGCCATGTCATCGACCTGGTGGCCGGCCGCGACTTCTCCGGCGAC
GGCCTGATCGAGGTGGCCGAGGTCCGCGACGGCGACCCCTGCCCGCGCGGCGACGGCGGCACCCTCGAGACCGCCCGCGG
CATCGAGATGGGCCACATCTTCCAGCTCGGCCGCAAGTACGCCGACGCCCTCGACCTCAAGGTCCTCGACGAGCAGGGCA
AGCTCGTCACGGTGACCATGGGCTCCTACGGCATCGGGCCCTCGCGCGCCGTGGCCGCCATCGCCGAGGGCACCCACGAC
GAGCTGGGCCTGGCCTGGCCGCGGGAGGTCGCGCCCGCCGACGTCCACATCGTCGCCACCGGCAAGGACGAGCACGTCTT
CGCGGCGGCCGAGCGGATCGCGCACGAGCTCGACAAGCAGGGTGTCGAGGTGCTCTACGACGACCGGCCCAAGGTCAGCC
CGGGGGTGAAGTTCAAGGACGCCGAGCTGATCGGTGTCCCGACGATCGTCGTGGTCGGCAAGGGCCTCGCCGCGGGCACG
ATCGAGGTCAAGGACCGACGTACCGGCCAGCGGCAGGACGTGCCGGCCGACCACCTGGTCGACCGGGTGATCGCCATCGT
GAGGGCATGA

Upstream 100 bases:

>100_bases
AACCCGGGCGAAGGTTGCAGTCCCGAACAAGTAGCCTTCTCCGCATGATCTCACCCCACGAAGCCGCTCGCTGCGCTCGC
GCCTCCGCGGGGGCCCCGAG

Downstream 100 bases:

>100_bases
ACGCCCCCGGCTCGCTCGACGCGGTCGTCTTCGACTGGGGCGGCACGCTCACGGCCTGGCACGACATCGACTTCCACGCG
GAGTCGCTGGCGCTCGCCCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MIMRMSSLFVRTLREDPADAEVPSHRLLVRAGYIRRAAPGIYTWLPLGLRVLRKIENVIREEMDAIGAQEMLFPALLPRE
PYEATNRWTEYGDGIFRLQDRKGADYLLGPTHEEMFTLVVKDLYSSYKDLPLSIYQIQTKYRDEARPRAGLLRGREFVMK
DSYSFDVDDAGLDVSYQKHRDAYVRIFDRLGFEYVIVEAMSGAMGGSKSEEFLAKASVGEDTYVRCTLCDYAANVEAVHS
PPIPPVPYDDAPAAHAEQTPDTPTIETLVAHLNERFPREDRPWAASDTLKNVVFSVHHPDGATEALAVGLPGDREVDAKR
LEAHLGEGVVFEPFGEADFAARPTLAKGYIGPGALGEKKPAGVRYLLDPRVVEGTRWVTGADAAGSHVIDLVAGRDFSGD
GLIEVAEVRDGDPCPRGDGGTLETARGIEMGHIFQLGRKYADALDLKVLDEQGKLVTVTMGSYGIGPSRAVAAIAEGTHD
ELGLAWPREVAPADVHIVATGKDEHVFAAAERIAHELDKQGVEVLYDDRPKVSPGVKFKDAELIGVPTIVVVGKGLAAGT
IEVKDRRTGQRQDVPADHLVDRVIAIVRA

Sequences:

>Translated_589_residues
MIMRMSSLFVRTLREDPADAEVPSHRLLVRAGYIRRAAPGIYTWLPLGLRVLRKIENVIREEMDAIGAQEMLFPALLPRE
PYEATNRWTEYGDGIFRLQDRKGADYLLGPTHEEMFTLVVKDLYSSYKDLPLSIYQIQTKYRDEARPRAGLLRGREFVMK
DSYSFDVDDAGLDVSYQKHRDAYVRIFDRLGFEYVIVEAMSGAMGGSKSEEFLAKASVGEDTYVRCTLCDYAANVEAVHS
PPIPPVPYDDAPAAHAEQTPDTPTIETLVAHLNERFPREDRPWAASDTLKNVVFSVHHPDGATEALAVGLPGDREVDAKR
LEAHLGEGVVFEPFGEADFAARPTLAKGYIGPGALGEKKPAGVRYLLDPRVVEGTRWVTGADAAGSHVIDLVAGRDFSGD
GLIEVAEVRDGDPCPRGDGGTLETARGIEMGHIFQLGRKYADALDLKVLDEQGKLVTVTMGSYGIGPSRAVAAIAEGTHD
ELGLAWPREVAPADVHIVATGKDEHVFAAAERIAHELDKQGVEVLYDDRPKVSPGVKFKDAELIGVPTIVVVGKGLAAGT
IEVKDRRTGQRQDVPADHLVDRVIAIVRA
>Mature_589_residues
MIMRMSSLFVRTLREDPADAEVPSHRLLVRAGYIRRAAPGIYTWLPLGLRVLRKIENVIREEMDAIGAQEMLFPALLPRE
PYEATNRWTEYGDGIFRLQDRKGADYLLGPTHEEMFTLVVKDLYSSYKDLPLSIYQIQTKYRDEARPRAGLLRGREFVMK
DSYSFDVDDAGLDVSYQKHRDAYVRIFDRLGFEYVIVEAMSGAMGGSKSEEFLAKASVGEDTYVRCTLCDYAANVEAVHS
PPIPPVPYDDAPAAHAEQTPDTPTIETLVAHLNERFPREDRPWAASDTLKNVVFSVHHPDGATEALAVGLPGDREVDAKR
LEAHLGEGVVFEPFGEADFAARPTLAKGYIGPGALGEKKPAGVRYLLDPRVVEGTRWVTGADAAGSHVIDLVAGRDFSGD
GLIEVAEVRDGDPCPRGDGGTLETARGIEMGHIFQLGRKYADALDLKVLDEQGKLVTVTMGSYGIGPSRAVAAIAEGTHD
ELGLAWPREVAPADVHIVATGKDEHVFAAAERIAHELDKQGVEVLYDDRPKVSPGVKFKDAELIGVPTIVVVGKGLAAGT
IEVKDRRTGQRQDVPADHLVDRVIAIVRA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=230, Percent_Identity=43.9130434782609, Blast_Score=202, Evalue=5e-52,
Organism=Escherichia coli, GI1786392, Length=599, Percent_Identity=40.0667779632721, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI115532348, Length=252, Percent_Identity=34.9206349206349, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320931, Length=233, Percent_Identity=41.2017167381974, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24656200, Length=223, Percent_Identity=35.8744394618834, Blast_Score=156, Evalue=4e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_NOCSJ (A1SLL4)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924386.1
- ProteinModelPortal:   A1SLL4
- SMR:   A1SLL4
- STRING:   A1SLL4
- GeneID:   4599060
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3197
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A1SLL4
- ProtClustDB:   PRK09194
- BioCyc:   NSP35761:NOCA_3197-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64584; Mature: 64584

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II; PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMRMSSLFVRTLREDPADAEVPSHRLLVRAGYIRRAAPGIYTWLPLGLRVLRKIENVIR
CEEEHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EEMDAIGAQEMLFPALLPREPYEATNRWTEYGDGIFRLQDRKGADYLLGPTHEEMFTLVV
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHH
KDLYSSYKDLPLSIYQIQTKYRDEARPRAGLLRGREFVMKDSYSFDVDDAGLDVSYQKHR
HHHHHHHCCCCEEEEEEHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHH
DAYVRIFDRLGFEYVIVEAMSGAMGGSKSEEFLAKASVGEDTYVRCTLCDYAANVEAVHS
HHHHHHHHHCCCEEEEEEHHHCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCEECC
PPIPPVPYDDAPAAHAEQTPDTPTIETLVAHLNERFPREDRPWAASDTLKNVVFSVHHPD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCEEEEECCC
GATEALAVGLPGDREVDAKRLEAHLGEGVVFEPFGEADFAARPTLAKGYIGPGALGEKKP
CCCCEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHCCCCCCCCCCCCCC
AGVRYLLDPRVVEGTRWVTGADAAGSHVIDLVAGRDFSGDGLIEVAEVRDGDPCPRGDGG
CCEEEEECCEEECCCEEEECCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCC
TLETARGIEMGHIFQLGRKYADALDLKVLDEQGKLVTVTMGSYGIGPSRAVAAIAEGTHD
CCCHHCCCCHHHHHHHHHHHHHHHCEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCC
ELGLAWPREVAPADVHIVATGKDEHVFAAAERIAHELDKQGVEVLYDDRPKVSPGVKFKD
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEECC
AELIGVPTIVVVGKGLAAGTIEVKDRRTGQRQDVPADHLVDRVIAIVRA
CEEECCCEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIMRMSSLFVRTLREDPADAEVPSHRLLVRAGYIRRAAPGIYTWLPLGLRVLRKIENVIR
CEEEHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EEMDAIGAQEMLFPALLPREPYEATNRWTEYGDGIFRLQDRKGADYLLGPTHEEMFTLVV
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHH
KDLYSSYKDLPLSIYQIQTKYRDEARPRAGLLRGREFVMKDSYSFDVDDAGLDVSYQKHR
HHHHHHHCCCCEEEEEEHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHH
DAYVRIFDRLGFEYVIVEAMSGAMGGSKSEEFLAKASVGEDTYVRCTLCDYAANVEAVHS
HHHHHHHHHCCCEEEEEEHHHCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCEECC
PPIPPVPYDDAPAAHAEQTPDTPTIETLVAHLNERFPREDRPWAASDTLKNVVFSVHHPD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCEEEEECCC
GATEALAVGLPGDREVDAKRLEAHLGEGVVFEPFGEADFAARPTLAKGYIGPGALGEKKP
CCCCEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHCCCCCCCCCCCCCC
AGVRYLLDPRVVEGTRWVTGADAAGSHVIDLVAGRDFSGDGLIEVAEVRDGDPCPRGDGG
CCEEEEECCEEECCCEEEECCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCC
TLETARGIEMGHIFQLGRKYADALDLKVLDEQGKLVTVTMGSYGIGPSRAVAAIAEGTHD
CCCHHCCCCHHHHHHHHHHHHHHHCEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCC
ELGLAWPREVAPADVHIVATGKDEHVFAAAERIAHELDKQGVEVLYDDRPKVSPGVKFKD
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEECC
AELIGVPTIVVVGKGLAAGTIEVKDRRTGQRQDVPADHLVDRVIAIVRA
CEEECCCEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA