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The map label for this gene is ltaA [H]

Identifier: 119717318

GI number: 119717318

Start: 3296240

End: 3297235

Strand: Reverse

Name: ltaA [H]

Synonym: Noca_3094

Alternate gene names: 119717318

Gene position: 3297235-3296240 (Counterclockwise)

Preceding gene: 119717319

Following gene: 119717309

Centisome position: 66.13

GC content: 74.8

Gene sequence:

>996_bases
GTGATCGACCTGCGCAGCGACACCCTGACCAGGCCGACCGACGCGATGCGCCAGGCGATGGCGCGCGCCGAGGTCGGTGA
CGACGTGTACGGCGAGGACCCGACGGTCGCGCGCCTCGAGGAGCGGGTCGCCGGCCTGCTCGGCCACGAGGCCGCGCTGT
TCACCCCGACCGGGTCGATGGCCAACGTGCTGGCCGTGCGCTCGCTGGTCGGGCCGGGCCAGGAGGTGCTCTGCGAGGCC
AGCGCGCACATCGCCCGCGCCGAGCTCGGCGCGCACGGTGCCTACACGGGCCTGACCATGCGCACCTGGATCCACCCGCG
CGGCCAGGTCGACCTGGCGGCGATCCGCACGATCTTCGCCCCCGACATGGGCCCGTTCTTCGTGCCGACCGCGGCCATCT
CGGTCGAGAACACCCACAACTTCGCCGGGGGAGCGGTGCTGCCGCTCGACGACCTGCGCGACCTGCGCGAGTACGCCACC
GCAGCCGGCGTCGGCGTGCACGTCGACGGCGCCCGGATCTGGAACGCGCACGTGGCGACCGGCACGCCGCTCGCGGAGTA
CGGCGCGATCGCCGACGTGCTGTCCGTGTGCCTCTCCAAGGGCCTCGGCGCCCCGGTCGGCTCCCTGATGGTGGGCAGCA
GCGACGCGATCGCCGAGGCTCGGGTGTGGCGCAAGCGGATGGGCGGCGGGATGCGCCAGGTGGGCATCCTGGCCGCGGCC
GGGCTGCACGCGCTCGACCACCACCTCGAGCGGCTGGCCGACGACCACGAGCACGCCCGGCTCCTGGCCGAGGCCTGCGG
CATCGACCCGGCGGAGGTCGACACCAACATCGTGGTCGTCCACCGCGCCGACGCCGCCGACTTCGTCGCCGCCGCCCGGG
ACGAAGGGGTGCTCGTCTCCGCGGTCGGCCCCACCGCCGTACGCCTGGTCACGCACCTCGACGTGACCCGCGAGGACGCC
GAGAAGGCCGCGGCGGTGCTGGCCCGCCTGGCGTGA

Upstream 100 bases:

>100_bases
GCACCCGCTTCGCCCGCCCGGGCACCTGGTCGGTGCGGGCGCGCACCACACAGGGGTCCGCCACGTCGGGCTGGTCGCCC
GCCGTGGTAGTCAAGACCTC

Downstream 100 bases:

>100_bases
CGCGTGCCTAGGACGCACCTACCAGCGACACGTCGCTGATCGCGGTGTAGTCGCGGGCCGCCCGGCCCTGGCCCGGCGCC
GAGACGGCGACCAGCCGGAG

Product: L-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIDLRSDTLTRPTDAMRQAMARAEVGDDVYGEDPTVARLEERVAGLLGHEAALFTPTGSMANVLAVRSLVGPGQEVLCEA
SAHIARAELGAHGAYTGLTMRTWIHPRGQVDLAAIRTIFAPDMGPFFVPTAAISVENTHNFAGGAVLPLDDLRDLREYAT
AAGVGVHVDGARIWNAHVATGTPLAEYGAIADVLSVCLSKGLGAPVGSLMVGSSDAIAEARVWRKRMGGGMRQVGILAAA
GLHALDHHLERLADDHEHARLLAEACGIDPAEVDTNIVVVHRADAADFVAAARDEGVLVSAVGPTAVRLVTHLDVTREDA
EKAAAVLARLA

Sequences:

>Translated_331_residues
MIDLRSDTLTRPTDAMRQAMARAEVGDDVYGEDPTVARLEERVAGLLGHEAALFTPTGSMANVLAVRSLVGPGQEVLCEA
SAHIARAELGAHGAYTGLTMRTWIHPRGQVDLAAIRTIFAPDMGPFFVPTAAISVENTHNFAGGAVLPLDDLRDLREYAT
AAGVGVHVDGARIWNAHVATGTPLAEYGAIADVLSVCLSKGLGAPVGSLMVGSSDAIAEARVWRKRMGGGMRQVGILAAA
GLHALDHHLERLADDHEHARLLAEACGIDPAEVDTNIVVVHRADAADFVAAARDEGVLVSAVGPTAVRLVTHLDVTREDA
EKAAAVLARLA
>Mature_331_residues
MIDLRSDTLTRPTDAMRQAMARAEVGDDVYGEDPTVARLEERVAGLLGHEAALFTPTGSMANVLAVRSLVGPGQEVLCEA
SAHIARAELGAHGAYTGLTMRTWIHPRGQVDLAAIRTIFAPDMGPFFVPTAAISVENTHNFAGGAVLPLDDLRDLREYAT
AAGVGVHVDGARIWNAHVATGTPLAEYGAIADVLSVCLSKGLGAPVGSLMVGSSDAIAEARVWRKRMGGGMRQVGILAAA
GLHALDHHLERLADDHEHARLLAEACGIDPAEVDTNIVVVHRADAADFVAAARDEGVLVSAVGPTAVRLVTHLDVTREDA
EKAAAVLARLA

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=337, Percent_Identity=44.5103857566766, Blast_Score=237, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI193206522, Length=348, Percent_Identity=39.9425287356322, Blast_Score=210, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI193206520, Length=348, Percent_Identity=39.9425287356322, Blast_Score=210, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI193206524, Length=348, Percent_Identity=39.9425287356322, Blast_Score=210, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6320789, Length=335, Percent_Identity=38.8059701492537, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI21355439, Length=328, Percent_Identity=41.4634146341463, Blast_Score=196, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 34677; Mature: 34677

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLRSDTLTRPTDAMRQAMARAEVGDDVYGEDPTVARLEERVAGLLGHEAALFTPTGSM
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHH
ANVLAVRSLVGPGQEVLCEASAHIARAELGAHGAYTGLTMRTWIHPRGQVDLAAIRTIFA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHC
PDMGPFFVPTAAISVENTHNFAGGAVLPLDDLRDLREYATAAGVGVHVDGARIWNAHVAT
CCCCCCCCCEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHCCCEEEECCCEEEEEEECC
GTPLAEYGAIADVLSVCLSKGLGAPVGSLMVGSSDAIAEARVWRKRMGGGMRQVGILAAA
CCCHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHH
GLHALDHHLERLADDHEHARLLAEACGIDPAEVDTNIVVVHRADAADFVAAARDEGVLVS
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCEEEEEECCHHHHHHHHCCCCEEEE
AVGPTAVRLVTHLDVTREDAEKAAAVLARLA
ECCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDLRSDTLTRPTDAMRQAMARAEVGDDVYGEDPTVARLEERVAGLLGHEAALFTPTGSM
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHH
ANVLAVRSLVGPGQEVLCEASAHIARAELGAHGAYTGLTMRTWIHPRGQVDLAAIRTIFA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHC
PDMGPFFVPTAAISVENTHNFAGGAVLPLDDLRDLREYATAAGVGVHVDGARIWNAHVAT
CCCCCCCCCEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHCCCEEEECCCEEEEEEECC
GTPLAEYGAIADVLSVCLSKGLGAPVGSLMVGSSDAIAEARVWRKRMGGGMRQVGILAAA
CCCHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHH
GLHALDHHLERLADDHEHARLLAEACGIDPAEVDTNIVVVHRADAADFVAAARDEGVLVS
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCEEEEEECCHHHHHHHHCCCCEEEE
AVGPTAVRLVTHLDVTREDAEKAAAVLARLA
ECCHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]