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The map label for this gene is hisH [H]

Identifier: 119717266

GI number: 119717266

Start: 3239663

End: 3240301

Strand: Reverse

Name: hisH [H]

Synonym: Noca_3042

Alternate gene names: 119717266

Gene position: 3240301-3239663 (Counterclockwise)

Preceding gene: 119717267

Following gene: 119717265

Centisome position: 64.99

GC content: 73.71

Gene sequence:

>639_bases
GTGACCCGTCCGAACGTGGTGGTCCTCGACTACGGCTCGGGGAACCTCCGCTCCGCCGTGCGCGCGGTCGAACGCGCCGG
CGCCGAGGTCACGCTGACCGCCGACCGCGCGCTGGCCCAGGAGGCCGACGGCCTGCTGGTTCCGGGCGTGGGCGCCTTCG
CCGCCTGCATGGCCGGCCTGCGCGCGGTGCGCGGCCACGAGGTGATCGGCCGGCGACTCGCCGGCGGCCGCCCCGTCCTC
GGCATCTGCGTGGGCATGCAGATCCTCTTCGAGCGCGGGGTCGAGCACGGGGTCGAGACCGAGGGCTGCGGCGAGTGGCC
CGGCGTGGTGGAGCGGCTGCAGGCGCCCGTCGTACCCCACATGGGTTGGAACACCGTGGACGTGGCCGCTGCATCGACCC
TGTTCGAGGGTGTCGAGGACGAGCGGTTCTACTTCGTGCACTCCTACGGGGTGCGGGACTGGACGCTGGAGACCAACGAC
CGCACCCGGGCGCCGCTGGTGCACTGGACCTCGCACGGCGGGGACCGGTTCGTGGCCGCGGTCGAGAACGGGCCGCTCAG
CGCCACCCAGTTCCACCCCGAGAAGTCCGGCGACGCCGGCGCGGCGCTGCTGCGCAACTGGGTCGGGTCCCTGTCATGA

Upstream 100 bases:

>100_bases
ACATCGTGGAGACCCAGTTCAAGGCGGTCGCCCGGGCCTTCCGCGACGCCGTCGCGCTCGACCCGCGCGAGACCGGGATC
CCCAGCACCAAGGGCGCTTT

Downstream 100 bases:

>100_bases
GGGTGGAGGTGACGGTGCCGTGAGCAAGGAGCGGGCCCGGCGCCGGGCCGAGCGCGAGCGGGAGGCCGCGGCGGTCGCGG
CGGCCCGGGCGGCGGAGGCC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTRPNVVVLDYGSGNLRSAVRAVERAGAEVTLTADRALAQEADGLLVPGVGAFAACMAGLRAVRGHEVIGRRLAGGRPVL
GICVGMQILFERGVEHGVETEGCGEWPGVVERLQAPVVPHMGWNTVDVAAASTLFEGVEDERFYFVHSYGVRDWTLETND
RTRAPLVHWTSHGGDRFVAAVENGPLSATQFHPEKSGDAGAALLRNWVGSLS

Sequences:

>Translated_212_residues
MTRPNVVVLDYGSGNLRSAVRAVERAGAEVTLTADRALAQEADGLLVPGVGAFAACMAGLRAVRGHEVIGRRLAGGRPVL
GICVGMQILFERGVEHGVETEGCGEWPGVVERLQAPVVPHMGWNTVDVAAASTLFEGVEDERFYFVHSYGVRDWTLETND
RTRAPLVHWTSHGGDRFVAAVENGPLSATQFHPEKSGDAGAALLRNWVGSLS
>Mature_211_residues
TRPNVVVLDYGSGNLRSAVRAVERAGAEVTLTADRALAQEADGLLVPGVGAFAACMAGLRAVRGHEVIGRRLAGGRPVLG
ICVGMQILFERGVEHGVETEGCGEWPGVVERLQAPVVPHMGWNTVDVAAASTLFEGVEDERFYFVHSYGVRDWTLETNDR
TRAPLVHWTSHGGDRFVAAVENGPLSATQFHPEKSGDAGAALLRNWVGSLS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=210, Percent_Identity=40.4761904761905, Blast_Score=124, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=217, Percent_Identity=33.6405529953917, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22642; Mature: 22511

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00059 ADH_ZINC ; PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPNVVVLDYGSGNLRSAVRAVERAGAEVTLTADRALAQEADGLLVPGVGAFAACMAGL
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCCCEEECCHHHHHHHHHHH
RAVRGHEVIGRRLAGGRPVLGICVGMQILFERGVEHGVETEGCGEWPGVVERLQAPVVPH
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
MGWNTVDVAAASTLFEGVEDERFYFVHSYGVRDWTLETNDRTRAPLVHWTSHGGDRFVAA
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEECCCCCEEEEE
VENGPLSATQFHPEKSGDAGAALLRNWVGSLS
ECCCCCCCEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRPNVVVLDYGSGNLRSAVRAVERAGAEVTLTADRALAQEADGLLVPGVGAFAACMAGL
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCCCEEECCHHHHHHHHHHH
RAVRGHEVIGRRLAGGRPVLGICVGMQILFERGVEHGVETEGCGEWPGVVERLQAPVVPH
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
MGWNTVDVAAASTLFEGVEDERFYFVHSYGVRDWTLETNDRTRAPLVHWTSHGGDRFVAA
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEECCCCCEEEEE
VENGPLSATQFHPEKSGDAGAALLRNWVGSLS
ECCCCCCCEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA