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The map label for this gene is lipA

Identifier: 119716526

GI number: 119716526

Start: 2449078

End: 2450028

Strand: Reverse

Name: lipA

Synonym: Noca_2299

Alternate gene names: 119716526

Gene position: 2450028-2449078 (Counterclockwise)

Preceding gene: 119716527

Following gene: 119716525

Centisome position: 49.14

GC content: 69.72

Gene sequence:

>951_bases
GTGACGCAAGCTCCCCCCTCCCCACAAGCAGAGGGCCGCAAGCTGCTCCGGCTCGAGGCACGCAACTCGGAGACCCCGAT
CGAGCGCAAGCCCGAGTGGATCAAGACCCGAGCGAAGATGGGTCCGGAGTACCAGCACCTCCAGAACCTGGTGAAGTCCG
AGGGACTGCACACCGTGTGCCAGGAGGCCGGCTGCCCCAACATCTACGAGTGCTGGGAGGACCGCGAGGCGACCTTCCTG
ATCGGCGGCGAGCAGTGCACCCGGCGCTGCGACTTCTGCCAGATCGACACCGGCCGGCCGAAGCCGCTGGACCGCGACGA
GCCGCGGCGGGTCGCGGAGTCGGTGCGCACGATGGGCCTGAGGTACGCCACCATCACCGGCGTCACGCGCGACGACCTGA
TCGACGAGGGCGCCTGGCTGTACGCCGAGACCGTGCGCCAGATCCACGAGCTGAACCCGGGCATCGGCGTGGAGAACCTG
ATCCCCGACTTCAGCGGCAAGCCGGACCTGCTCGCCCAGGTCTTCGAGTCGCGGCCCGAGGTGCTCGCCCACAACCTCGA
GACCGTGCCGCGGATCTTCAAGCGGATCCGTCCGGCGTTCCGCTACGACCGCTCGCTCGACGTGCTGACCCAGGCTCGCG
ACTTCGGCCTGGTCACGAAGTCCAACCTGATCCTCGGCCTCGGCGAGACCCGCGAGGAGGTCTCGCAGGCCCTGCGCGAC
CTGCACGCCGCGGGCTGCGAGCTGCTCACCATCACCCAGTACCTCCGCCCCTCGCCGCGGCACCACCCGGTCGAGCGCTG
GGTCAAGCCGGAGGAGTTCGTGGAGCTCAAGGACGAGGCCGACGAGGTCGGGTTCACCGGGGTGATGTCCGGGCCGCTCG
TCCGTTCGTCCTACCGCGCCGGTCGGCTGTACCGTCAGGCTGTCGAGGCCCGCGAGGGCGTCACGGCCTGA

Upstream 100 bases:

>100_bases
CGACTACGAGCCGCGCCCCGACCCGGCCAAGCAGCCGCGGATCGAGCTGCTCACTCCCTGAGGCGGGACCGCCTCCACCC
GCCGCGTACAGTTGGCCAAC

Downstream 100 bases:

>100_bases
TCCCGACCGAACGACTCAGTGAGGCAGCACCAGAAGATGGCGAAGGACCCCAAGCCCGTCGACCCGGACAAGATGAGCCG
GCGCAAGCAGTTCGTCGAGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTQAPPSPQAEGRKLLRLEARNSETPIERKPEWIKTRAKMGPEYQHLQNLVKSEGLHTVCQEAGCPNIYECWEDREATFL
IGGEQCTRRCDFCQIDTGRPKPLDRDEPRRVAESVRTMGLRYATITGVTRDDLIDEGAWLYAETVRQIHELNPGIGVENL
IPDFSGKPDLLAQVFESRPEVLAHNLETVPRIFKRIRPAFRYDRSLDVLTQARDFGLVTKSNLILGLGETREEVSQALRD
LHAAGCELLTITQYLRPSPRHHPVERWVKPEEFVELKDEADEVGFTGVMSGPLVRSSYRAGRLYRQAVEAREGVTA

Sequences:

>Translated_316_residues
MTQAPPSPQAEGRKLLRLEARNSETPIERKPEWIKTRAKMGPEYQHLQNLVKSEGLHTVCQEAGCPNIYECWEDREATFL
IGGEQCTRRCDFCQIDTGRPKPLDRDEPRRVAESVRTMGLRYATITGVTRDDLIDEGAWLYAETVRQIHELNPGIGVENL
IPDFSGKPDLLAQVFESRPEVLAHNLETVPRIFKRIRPAFRYDRSLDVLTQARDFGLVTKSNLILGLGETREEVSQALRD
LHAAGCELLTITQYLRPSPRHHPVERWVKPEEFVELKDEADEVGFTGVMSGPLVRSSYRAGRLYRQAVEAREGVTA
>Mature_315_residues
TQAPPSPQAEGRKLLRLEARNSETPIERKPEWIKTRAKMGPEYQHLQNLVKSEGLHTVCQEAGCPNIYECWEDREATFLI
GGEQCTRRCDFCQIDTGRPKPLDRDEPRRVAESVRTMGLRYATITGVTRDDLIDEGAWLYAETVRQIHELNPGIGVENLI
PDFSGKPDLLAQVFESRPEVLAHNLETVPRIFKRIRPAFRYDRSLDVLTQARDFGLVTKSNLILGLGETREEVSQALRDL
HAAGCELLTITQYLRPSPRHHPVERWVKPEEFVELKDEADEVGFTGVMSGPLVRSSYRAGRLYRQAVEAREGVTA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=292, Percent_Identity=42.1232876712329, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=42.2764227642276, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1786846, Length=297, Percent_Identity=43.4343434343434, Blast_Score=251, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI32564533, Length=292, Percent_Identity=39.7260273972603, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324770, Length=299, Percent_Identity=42.1404682274247, Blast_Score=225, Evalue=7e-60,
Organism=Drosophila melanogaster, GI221513272, Length=289, Percent_Identity=42.2145328719723, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_NOCSJ (A1SJ19)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_923491.1
- ProteinModelPortal:   A1SJ19
- STRING:   A1SJ19
- GeneID:   4595847
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_2299
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   NSP35761:NOCA_2299-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35917; Mature: 35786

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAPPSPQAEGRKLLRLEARNSETPIERKPEWIKTRAKMGPEYQHLQNLVKSEGLHTVC
CCCCCCCCCHHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
QEAGCPNIYECWEDREATFLIGGEQCTRRCDFCQIDTGRPKPLDRDEPRRVAESVRTMGL
HHCCCCCHHHHHCCCCCEEEECCHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
RYATITGVTRDDLIDEGAWLYAETVRQIHELNPGIGVENLIPDFSGKPDLLAQVFESRPE
HHHHEECCHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHCCHH
VLAHNLETVPRIFKRIRPAFRYDRSLDVLTQARDFGLVTKSNLILGLGETREEVSQALRD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCEEEECCCCHHHHHHHHHH
LHAAGCELLTITQYLRPSPRHHPVERWVKPEEFVELKDEADEVGFTGVMSGPLVRSSYRA
HHHCCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHH
GRLYRQAVEAREGVTA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQAPPSPQAEGRKLLRLEARNSETPIERKPEWIKTRAKMGPEYQHLQNLVKSEGLHTVC
CCCCCCCCHHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
QEAGCPNIYECWEDREATFLIGGEQCTRRCDFCQIDTGRPKPLDRDEPRRVAESVRTMGL
HHCCCCCHHHHHCCCCCEEEECCHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
RYATITGVTRDDLIDEGAWLYAETVRQIHELNPGIGVENLIPDFSGKPDLLAQVFESRPE
HHHHEECCHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHCCHH
VLAHNLETVPRIFKRIRPAFRYDRSLDVLTQARDFGLVTKSNLILGLGETREEVSQALRD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCEEEECCCCHHHHHHHHHH
LHAAGCELLTITQYLRPSPRHHPVERWVKPEEFVELKDEADEVGFTGVMSGPLVRSSYRA
HHHCCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHH
GRLYRQAVEAREGVTA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA