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The map label for this gene is fliM [H]

Identifier: 119714993

GI number: 119714993

Start: 794077

End: 795084

Strand: Reverse

Name: fliM [H]

Synonym: Noca_0746

Alternate gene names: 119714993

Gene position: 795084-794077 (Counterclockwise)

Preceding gene: 119714994

Following gene: 119714992

Centisome position: 15.95

GC content: 72.82

Gene sequence:

>1008_bases
GTGCAGACATCCCTCAGGAACGCGCCGGCCGGGCCGATGAGAGTCATCGTGGCCTCCTCCGACGCTTCCGTCACGGACCC
CGGCGCCGCCCCCGCGGCGGACCGGCGGGCCCGGCGCGCCTCCGCGACCCCGCAGCCGTACGACTTCCGGCGTCCGATCC
AGCTCTCCCGCGAGCACTCGCGGATGCTGCAGCTGGCCTTCGACGACTTCTGCCGGCAGGCGACGACCGTCTTCACCTCG
TCGCTGCGGACCGTGTGCGGGGTCAGCCTGGCCTCGATCGAGCAGCGCACGTACGCCGAGTACATCGACTCGCTGGACGC
GTCGACGTACCTGACCTCGTTCTCCGCGGAGCCGCTCTTCGACCGGGGCGTCTTCGAGATCCCCCTGCTCGCGGTGATGA
CCTGCGTGGACCACATGCTCGGCGGCCCCGGCCGCCTCGAGCAGCCGGAGCGGCCGCTGACCGAGATCGAGGCCGGCGTC
GCGAGCGGCATGATCGAGCGCCTGCTCGGCGAGCTGCGCTACTCGATGGCCTCGATCGTCGCCTTCGAGCCGGTCGTCAA
GGGGGTGGAGTACAGCCCCCAGTTCGTCCAGGTCGCCGGCGCCTCCGACGTGATGGTGGCCGCCAGCCTGGAGCTGCGGA
TCAACGACCGTACGTTCCGGATGACGATGTGCATGCCGTTCTCGAGCCTGCTCCCCCACCTGCTCCGCGCCGCGGCACCC
GCACCCGTCTCCGAGCGCGAGCGCGCCCAGCGCGCCCAGGCGGCCGCCGAGCTCGCCGACCAGTTCCAGCGGGTGCCCGT
CGAGGTCGCCGTGAGTCTGCGCCCGACCACGCTCAGCCCGATGGAGCTCGCCGCGCTGCGCCCCGGCGACGCGCTCCGGC
TCCGGCACCCCGCCGCGGCCCCCCTCGAGGTCGTCGTCGACGGCACCTGTTTCGCGCACGCCACCCCCGGGACCAGCGGA
TTCCGGCTCGCCGCCCTGATCGTGGGCACCCCGAAGGAGAACGCATGA

Upstream 100 bases:

>100_bases
AGCCCGACGACCGGGGCCGGCAGTGCCCCCAGGACCAGGTCGCTCGCGGAGAGCGCGAGCTGACCCACCACCAGGTGGGA
GATCGTCGCCGGGACTTCTG

Downstream 100 bases:

>100_bases
CCACCGTGCAGACCACCGTCGACCTCGAGCTGGCGGTCAGCGCCGCGACCGCCGCCATCGCAGTGCTGCCGGCCGCAGAG
CCCCTCACCGCCGGTGCGCC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MQTSLRNAPAGPMRVIVASSDASVTDPGAAPAADRRARRASATPQPYDFRRPIQLSREHSRMLQLAFDDFCRQATTVFTS
SLRTVCGVSLASIEQRTYAEYIDSLDASTYLTSFSAEPLFDRGVFEIPLLAVMTCVDHMLGGPGRLEQPERPLTEIEAGV
ASGMIERLLGELRYSMASIVAFEPVVKGVEYSPQFVQVAGASDVMVAASLELRINDRTFRMTMCMPFSSLLPHLLRAAAP
APVSERERAQRAQAAAELADQFQRVPVEVAVSLRPTTLSPMELAALRPGDALRLRHPAAAPLEVVVDGTCFAHATPGTSG
FRLAALIVGTPKENA

Sequences:

>Translated_335_residues
MQTSLRNAPAGPMRVIVASSDASVTDPGAAPAADRRARRASATPQPYDFRRPIQLSREHSRMLQLAFDDFCRQATTVFTS
SLRTVCGVSLASIEQRTYAEYIDSLDASTYLTSFSAEPLFDRGVFEIPLLAVMTCVDHMLGGPGRLEQPERPLTEIEAGV
ASGMIERLLGELRYSMASIVAFEPVVKGVEYSPQFVQVAGASDVMVAASLELRINDRTFRMTMCMPFSSLLPHLLRAAAP
APVSERERAQRAQAAAELADQFQRVPVEVAVSLRPTTLSPMELAALRPGDALRLRHPAAAPLEVVVDGTCFAHATPGTSG
FRLAALIVGTPKENA
>Mature_335_residues
MQTSLRNAPAGPMRVIVASSDASVTDPGAAPAADRRARRASATPQPYDFRRPIQLSREHSRMLQLAFDDFCRQATTVFTS
SLRTVCGVSLASIEQRTYAEYIDSLDASTYLTSFSAEPLFDRGVFEIPLLAVMTCVDHMLGGPGRLEQPERPLTEIEAGV
ASGMIERLLGELRYSMASIVAFEPVVKGVEYSPQFVQVAGASDVMVAASLELRINDRTFRMTMCMPFSSLLPHLLRAAAP
APVSERERAQRAQAAAELADQFQRVPVEVAVSLRPTTLSPMELAALRPGDALRLRHPAAAPLEVVVDGTCFAHATPGTSG
FRLAALIVGTPKENA

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=306, Percent_Identity=23.8562091503268, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36200; Mature: 36200

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSLRNAPAGPMRVIVASSDASVTDPGAAPAADRRARRASATPQPYDFRRPIQLSREHS
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RMLQLAFDDFCRQATTVFTSSLRTVCGVSLASIEQRTYAEYIDSLDASTYLTSFSAEPLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
DRGVFEIPLLAVMTCVDHMLGGPGRLEQPERPLTEIEAGVASGMIERLLGELRYSMASIV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFEPVVKGVEYSPQFVQVAGASDVMVAASLELRINDRTFRMTMCMPFSSLLPHLLRAAAP
HHHHHHHCCCCCCCEEEECCCCCEEEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHCCC
APVSERERAQRAQAAAELADQFQRVPVEVAVSLRPTTLSPMELAALRPGDALRLRHPAAA
CCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHCCCCCCEEECCCCCC
PLEVVVDGTCFAHATPGTSGFRLAALIVGTPKENA
CEEEEECCCEEEECCCCCCCEEEEEEEEECCCCCC
>Mature Secondary Structure
MQTSLRNAPAGPMRVIVASSDASVTDPGAAPAADRRARRASATPQPYDFRRPIQLSREHS
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RMLQLAFDDFCRQATTVFTSSLRTVCGVSLASIEQRTYAEYIDSLDASTYLTSFSAEPLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
DRGVFEIPLLAVMTCVDHMLGGPGRLEQPERPLTEIEAGVASGMIERLLGELRYSMASIV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFEPVVKGVEYSPQFVQVAGASDVMVAASLELRINDRTFRMTMCMPFSSLLPHLLRAAAP
HHHHHHHCCCCCCCEEEECCCCCEEEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHCCC
APVSERERAQRAQAAAELADQFQRVPVEVAVSLRPTTLSPMELAALRPGDALRLRHPAAA
CCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHCCCCCCEEECCCCCC
PLEVVVDGTCFAHATPGTSGFRLAALIVGTPKENA
CEEEEECCCEEEECCCCCCCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]