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The map label for this gene is bglIIM [H]

Identifier: 119386545

GI number: 119386545

Start: 972636

End: 973571

Strand: Direct

Name: bglIIM [H]

Synonym: Pden_3838

Alternate gene names: 119386545

Gene position: 972636-973571 (Clockwise)

Preceding gene: 119386544

Following gene: 119386546

Centisome position: 56.22

GC content: 53.21

Gene sequence:

>936_bases
ATGCCGCACCTTTCCTGTGGCCTCAAGAATCACCGGAACAAAGACATGTCCCCGAAACAAGCCTTGGCTCTTCTCCGCTC
TGACCACGACGATGGTGCCACGGTTCATCGCTTGGAGCGAGACGCGAAAGCCGAGATCGCTTGCGAGCACAATCTCAAGT
TCATGCGTCGTTTGCCTGACGAATCCATGAGCCTGATCGTGACGTCACCGCCCTACAACATTGGAAAGTCTTATGAAAAG
CGCACATCGCAAGAAAAATATGTTGAGGATCAGGCGGCGTGCATTGCCGAAGCCGTACGTCTGCTCGCGCCAAACGGGTC
CATCTGCTGGCAGGTTGGCAACCACGTTGACTCAGGCGAGATATTCCCGCTAGACATCCTTCTCTACCCGCTCTTTAAGC
ACCACGGCCTGCAACTCCGCAATCGAATTGTTTGGACGTTCGGTCATGGGCTGCACTGCCAGAAGCGCTTTTCGGGTAGG
CATGAAACGATTCTATGGTTCACCAAATCTGAGGACTACACGTTCAACCTAGATCCTGTCCGCGTCCCCTCGAAGTATCC
GAACAAAAAGCACTTCAAAGGTCCGAACAAGGGCGAGCTTTCCAGCAATCCTCTTGGAAAGAACCCTTCCGATGTTTGGG
ATATTCCCAATGTCAAATCCAACCACGTTGAAAAGACCGATCATCCTTGCCAGTTCCCCATCGGACTGGTGGAGAGATTG
GTGTTGGCTTTGACAAACGAAGGCGAGAGCGTCCTCGATCCTTATCTTGGCGTTGGTTCGTCCGCGATTGCAGCGTTGAA
GCATGGAAGACATGCCTACGGCTGCGATCTCGATAAAGCCTATGTCGATATTGCATGGGATCGTATTCACCAGCTGCGCG
CAGGTACGCTCCGCACCCGCCCGATGAACAAACCCGTCTATGACCCCAATGCTTAG

Upstream 100 bases:

>100_bases
GTACGAGAGACACCTCTCGGGTGTCGCGTATCTGCAAGATGCGGGGTTGTCTTTATCGTCTGACACCAGATATCGTGTGA
GATACCGCCTGTTTGTCGTC

Downstream 100 bases:

>100_bases
TAGTGTCCATGAAGCTCGCATATACCTACGAAGATCACCACAGCGCGGGTGCCGAATGGGATCGGCGTGACCTGAAAGAA
TGGCTCACGGACGTTTTTGA

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.BglII; N(4)- cytosine-specific methyltransferase BglII [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MPHLSCGLKNHRNKDMSPKQALALLRSDHDDGATVHRLERDAKAEIACEHNLKFMRRLPDESMSLIVTSPPYNIGKSYEK
RTSQEKYVEDQAACIAEAVRLLAPNGSICWQVGNHVDSGEIFPLDILLYPLFKHHGLQLRNRIVWTFGHGLHCQKRFSGR
HETILWFTKSEDYTFNLDPVRVPSKYPNKKHFKGPNKGELSSNPLGKNPSDVWDIPNVKSNHVEKTDHPCQFPIGLVERL
VLALTNEGESVLDPYLGVGSSAIAALKHGRHAYGCDLDKAYVDIAWDRIHQLRAGTLRTRPMNKPVYDPNA

Sequences:

>Translated_311_residues
MPHLSCGLKNHRNKDMSPKQALALLRSDHDDGATVHRLERDAKAEIACEHNLKFMRRLPDESMSLIVTSPPYNIGKSYEK
RTSQEKYVEDQAACIAEAVRLLAPNGSICWQVGNHVDSGEIFPLDILLYPLFKHHGLQLRNRIVWTFGHGLHCQKRFSGR
HETILWFTKSEDYTFNLDPVRVPSKYPNKKHFKGPNKGELSSNPLGKNPSDVWDIPNVKSNHVEKTDHPCQFPIGLVERL
VLALTNEGESVLDPYLGVGSSAIAALKHGRHAYGCDLDKAYVDIAWDRIHQLRAGTLRTRPMNKPVYDPNA
>Mature_310_residues
PHLSCGLKNHRNKDMSPKQALALLRSDHDDGATVHRLERDAKAEIACEHNLKFMRRLPDESMSLIVTSPPYNIGKSYEKR
TSQEKYVEDQAACIAEAVRLLAPNGSICWQVGNHVDSGEIFPLDILLYPLFKHHGLQLRNRIVWTFGHGLHCQKRFSGRH
ETILWFTKSEDYTFNLDPVRVPSKYPNKKHFKGPNKGELSSNPLGKNPSDVWDIPNVKSNHVEKTDHPCQFPIGLVERLV
LALTNEGESVLDPYLGVGSSAIAALKHGRHAYGCDLDKAYVDIAWDRIHQLRAGTLRTRPMNKPVYDPNA

Specific function: This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=241, Percent_Identity=29.8755186721992, Blast_Score=114, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 35180; Mature: 35049

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHLSCGLKNHRNKDMSPKQALALLRSDHDDGATVHRLERDAKAEIACEHNLKFMRRLPD
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCHHEEEHHHHHHHHHHCCC
ESMSLIVTSPPYNIGKSYEKRTSQEKYVEDQAACIAEAVRLLAPNGSICWQVGNHVDSGE
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
IFPLDILLYPLFKHHGLQLRNRIVWTFGHGLHCQKRFSGRHETILWFTKSEDYTFNLDPV
EEEHHHHHHHHHHHCCCEECCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCEEECCCCE
RVPSKYPNKKHFKGPNKGELSSNPLGKNPSDVWDIPNVKSNHVEKTDHPCQFPIGLVERL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VLALTNEGESVLDPYLGVGSSAIAALKHGRHAYGCDLDKAYVDIAWDRIHQLRAGTLRTR
HHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PMNKPVYDPNA
CCCCCCCCCCC
>Mature Secondary Structure 
PHLSCGLKNHRNKDMSPKQALALLRSDHDDGATVHRLERDAKAEIACEHNLKFMRRLPD
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCHHEEEHHHHHHHHHHCCC
ESMSLIVTSPPYNIGKSYEKRTSQEKYVEDQAACIAEAVRLLAPNGSICWQVGNHVDSGE
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
IFPLDILLYPLFKHHGLQLRNRIVWTFGHGLHCQKRFSGRHETILWFTKSEDYTFNLDPV
EEEHHHHHHHHHHHCCCEECCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCEEECCCCE
RVPSKYPNKKHFKGPNKGELSSNPLGKNPSDVWDIPNVKSNHVEKTDHPCQFPIGLVERL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VLALTNEGESVLDPYLGVGSSAIAALKHGRHAYGCDLDKAYVDIAWDRIHQLRAGTLRTR
HHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PMNKPVYDPNA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9073062 [H]