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The map label for this gene is mcbR [H]

Identifier: 119386385

GI number: 119386385

Start: 825257

End: 825901

Strand: Direct

Name: mcbR [H]

Synonym: Pden_3677

Alternate gene names: 119386385

Gene position: 825257-825901 (Clockwise)

Preceding gene: 119386384

Following gene: 119386390

Centisome position: 47.7

GC content: 71.01

Gene sequence:

>645_bases
ATGACTGTTACGCGTTCCTCGGAAAGTTCGGCCCATGAGCGGCTTTATCGCAACCTGCGCCAGCGCATCATGCTGGGCGA
ACTGCCGCCGGGGCTGCCCCTGACCCTGCGCGGCATCGCCGCCGAGCATCGCGTCTCGATGACCCCCGCGCGCGAGGCGG
TGCGCCGTCTGGTGGCCGAAGGGGCGCTGTCGCTCTCCTCCTCGGGGCGGATCGCCACGCCGGAACTGTCGGTCGAGCGG
ATCGAGGAACTGGCCGCCCTGCGCGGGCTCATCGAGCCGGAACTGGCCGCTCGCGCCTTGCCGCGCGCCCACCAGGCGCT
GATCGAGCGCATGGCCGTCATCAACGCCGCCATCGGCGAGGCGGCCGAGCGTCACGACGCCGTGGGCTACATCCGCACCA
ATCTGGAGTTCCACCGCCTGCTTTACCTGCGTGCCCAGGCCCCGGCGATGCTGGCCATCGTCGAGACGGTCTGGCTGCAG
CTTGGCCCGACCATGCGGGCGCTGTACGGGCGGATGAAGCGCAACGAACCGCCGCGCCATCACCGCATGATCCTGGCCTC
GCTGCGGGCCGGGGATGAGCCGGGGCTGCGGCTGGCGGTCCGCGCCGATGTGACGCATGGGCTGCGCCATCTCGGCAGCG
CCTGA

Upstream 100 bases:

>100_bases
CGACTGGCTGCGCCAGCATGGGCATGAACTGCTGGCCTGGCGCTTCCGCTCCTGACCGGGGCGGCTTGACGGCGGCCGGA
TTTGTGATCACAGATGGCGC

Downstream 100 bases:

>100_bases
GGCCCCCGCGCCGACAGCCGGTCTTTCCGTTTTGCAAATACCCGAAAACCCGGCGATGCGGCGGGGCGCCGCCCGTGCCG
GCTCAGACCTCTTCGATGCG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTVTRSSESSAHERLYRNLRQRIMLGELPPGLPLTLRGIAAEHRVSMTPAREAVRRLVAEGALSLSSSGRIATPELSVER
IEELAALRGLIEPELAARALPRAHQALIERMAVINAAIGEAAERHDAVGYIRTNLEFHRLLYLRAQAPAMLAIVETVWLQ
LGPTMRALYGRMKRNEPPRHHRMILASLRAGDEPGLRLAVRADVTHGLRHLGSA

Sequences:

>Translated_214_residues
MTVTRSSESSAHERLYRNLRQRIMLGELPPGLPLTLRGIAAEHRVSMTPAREAVRRLVAEGALSLSSSGRIATPELSVER
IEELAALRGLIEPELAARALPRAHQALIERMAVINAAIGEAAERHDAVGYIRTNLEFHRLLYLRAQAPAMLAIVETVWLQ
LGPTMRALYGRMKRNEPPRHHRMILASLRAGDEPGLRLAVRADVTHGLRHLGSA
>Mature_213_residues
TVTRSSESSAHERLYRNLRQRIMLGELPPGLPLTLRGIAAEHRVSMTPAREAVRRLVAEGALSLSSSGRIATPELSVERI
EELAALRGLIEPELAARALPRAHQALIERMAVINAAIGEAAERHDAVGYIRTNLEFHRLLYLRAQAPAMLAIVETVWLQL
GPTMRALYGRMKRNEPPRHHRMILASLRAGDEPGLRLAVRADVTHGLRHLGSA

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=187, Percent_Identity=29.9465240641711, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 23679; Mature: 23548

Theoretical pI: Translated: 11.45; Mature: 11.45

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTRSSESSAHERLYRNLRQRIMLGELPPGLPLTLRGIAAEHRVSMTPAREAVRRLVAE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHH
GALSLSSSGRIATPELSVERIEELAALRGLIEPELAARALPRAHQALIERMAVINAAIGE
HHHCCCCCCCEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AAERHDAVGYIRTNLEFHRLLYLRAQAPAMLAIVETVWLQLGPTMRALYGRMKRNEPPRH
HHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHH
HRMILASLRAGDEPGLRLAVRADVTHGLRHLGSA
HHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCC
>Mature Secondary Structure 
TVTRSSESSAHERLYRNLRQRIMLGELPPGLPLTLRGIAAEHRVSMTPAREAVRRLVAE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHH
GALSLSSSGRIATPELSVERIEELAALRGLIEPELAARALPRAHQALIERMAVINAAIGE
HHHCCCCCCCEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AAERHDAVGYIRTNLEFHRLLYLRAQAPAMLAIVETVWLQLGPTMRALYGRMKRNEPPRH
HHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHH
HRMILASLRAGDEPGLRLAVRADVTHGLRHLGSA
HHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]